Study of marine noctilucence in marine is important to fishery, environmental monitoring and military affairs. A luminous bacterial strain D2 was isolated from the marine sediment samples collected near Donghai Island...Study of marine noctilucence in marine is important to fishery, environmental monitoring and military affairs. A luminous bacterial strain D2 was isolated from the marine sediment samples collected near Donghai Island in Zhanjiang, China. The primary cultural and luminescent conditions of luminous bacterium D2 which was identified as Vibrio sp. were determined in liquid culture. The results showed that pH 7.0, 35 ℃, with 2.0 % NaCI, were the best growth conditions, and pH5 - 6, 20℃, OD600 0.08, with 3.0 % NaCI, were the optimal luminescent conditions.展开更多
文摘采用超高效液相色谱-串联质谱(ultra-high-performance liquid chromatography-mass spectrometry,UHPLC-MS/MS)的非靶向代谢组学方法进行代谢物丰度检测和鉴定,结合多元统计方法比较健康对照组、晚期Luminal型乳腺癌(luminal advanced breast cancer,Luminal ABC)哌柏西利耐药组和敏感组患者中代谢谱趋势差异,以变量权重值(variable importance in projection,VIP)>1、P<0.05和差异倍数(fold change,FC)>1.2或<0.833标准筛选差异代谢物,对鉴定到的代谢物通过加权基因共表达网络分析(weighted gene co-expression network a nalysis,WGCNA)来确定与耐药相关的共表达模块,京都基因与基因组百科全书(kyoto encyclopedia of genes and genomes,KEGG)通路分析和代谢物集富集分析(metabolite set enrichment analysis,MSEA)识别与耐药相关的关键代谢通路和生物学功能。结果显示,共鉴定出583种代谢物,依据筛选标准筛选出54种差异代谢物,通过WGCNA分析构建出8个共表达模块。其中青绿色模块(MEturquoise)与哌柏西利耐药表型显著相关(r=0.46,P<0.05),共包含179种关键代谢物,且有22个关键代谢物同为差异代谢物,即精氨酸和苯丙氨酸等16种显著上调,胆碱和辛酸等6种显著下调。模块内整体代谢物与差异代谢物具有相似的代谢功能和生物学作用(P<0.05)。因此,Luminal ABC患者血清代谢水平与健康人群存在显著差异,结合WGCNA识别哌柏西利耐药相关的差异代谢物,以期为Luminal ABC哌柏西利耐药患者的代谢异常调控提供数据支持和研究依据。
文摘Study of marine noctilucence in marine is important to fishery, environmental monitoring and military affairs. A luminous bacterial strain D2 was isolated from the marine sediment samples collected near Donghai Island in Zhanjiang, China. The primary cultural and luminescent conditions of luminous bacterium D2 which was identified as Vibrio sp. were determined in liquid culture. The results showed that pH 7.0, 35 ℃, with 2.0 % NaCI, were the best growth conditions, and pH5 - 6, 20℃, OD600 0.08, with 3.0 % NaCI, were the optimal luminescent conditions.
