Objective To test the possibility of modification of human degenerative lumbar disc cells by the exogenous growth factor gene, transforming growth factor β_1 (TGF-β_ 1) cDNA, and the expression of the encoded prote...Objective To test the possibility of modification of human degenerative lumbar disc cells by the exogenous growth factor gene, transforming growth factor β_1 (TGF-β_ 1) cDNA, and the expression of the encoded protein. Methods Nucleus pulposus samples were surgically obtained from 8 patients with degenerat ive lumbar disc disease. The cells were cultured and directly infected by two a denoviral constructs, Ad/CMV-EGFP containing the enhanced green fluorecence pro tein (EGFP) gene (marker gene) and Ad/CMV-TGF-β_1 containing the potentially therapeutic TGF-β_1 gene. Transgene expression was analyzed by fluorescence production and immunohistochemical staining (Ad/CMV-TGF-β_1). Results Culture cells transducted by Ad/CMV-EGFP showed specific green fluorescence und er the fluoroscope and expression sustained for at least 4 weeks. When infe cted by Ad/CMV-TGF-β_1, approximally 30% of cultured cells were staind brown (+) with TGF-β_1 staining. Conclusion This study established the strategy of delivering a potentially therapeutic gene , TGF-β_1, by using an adenoviral vector to human degenerative lumbar interve rtebral disc cells.展开更多
12月15日,知名营养美容品牌Lumì在上海老码头举办"Lumi into your life"新闻发布会,宣布正式签约人气影视花旦杨幂为2012年品牌形象代言人,首支由杨幂领衔主演的花漾唯美风Lumì胶原蛋白TVC在现场惊艳首映,揭开令万...12月15日,知名营养美容品牌Lumì在上海老码头举办"Lumi into your life"新闻发布会,宣布正式签约人气影视花旦杨幂为2012年品牌形象代言人,首支由杨幂领衔主演的花漾唯美风Lumì胶原蛋白TVC在现场惊艳首映,揭开令万千女性如花绽放的胶原养美新’肌’密。近年来因出演古装穿越剧而人气飙升、荣升新"四小花旦"之首的杨幂,是继浙江卫视当红女主播朱丹之后。展开更多
目的藏药五味甘露是藏药浴关键组方之一,目前五味甘露挥发油的成分尚不明确,其作用机理也不清楚,因此文章旨在分析其挥发油成分以及探明其作用机理。方法基于《四部医典》等藏医药古籍文献对五味甘露进行发酵;利用超临界CO_(2)提取挥发...目的藏药五味甘露是藏药浴关键组方之一,目前五味甘露挥发油的成分尚不明确,其作用机理也不清楚,因此文章旨在分析其挥发油成分以及探明其作用机理。方法基于《四部医典》等藏医药古籍文献对五味甘露进行发酵;利用超临界CO_(2)提取挥发油;运用气相色谱质谱联用分析其挥发油成分;整合网络药理学预测其挥发性标志成分并分析其作用机理。结果从五味甘露挥发油中共鉴定出木香醇(10.51%)、柏木烯醇(5.51%)、γ-桉叶醇(2.36%)、桃拓酚(2.33%)、β-桉叶醇(2.28%)、榄香烯(1.59%)等176种化学成分;整合网络药理学预测五味甘露挥发性标志成分有3,7-二甲基-6-辛酸、γ-壬酸内酯、莪术双环烯酮、延胡索酮、γ-桉叶醇等,核心靶点有IL6、TNF、AKT1、IFNG、ALB、CCL2等,治疗类风湿性关节炎的生物学通路主要作用为side of membrane、lytic vacuole、lysosome等信号通路。结论本研究阐明了藏药五味甘露挥发油的化学成分,反映了中藏药“成分反映活性,活性指向功效”的思维;整合网络药理学预测藏药五味甘露挥发性标志成分并分析其作用机理,为临床前实验研究建立科学标准的质量评价体系提供参考依据及药理学理论基础。展开更多
文摘Objective To test the possibility of modification of human degenerative lumbar disc cells by the exogenous growth factor gene, transforming growth factor β_1 (TGF-β_ 1) cDNA, and the expression of the encoded protein. Methods Nucleus pulposus samples were surgically obtained from 8 patients with degenerat ive lumbar disc disease. The cells were cultured and directly infected by two a denoviral constructs, Ad/CMV-EGFP containing the enhanced green fluorecence pro tein (EGFP) gene (marker gene) and Ad/CMV-TGF-β_1 containing the potentially therapeutic TGF-β_1 gene. Transgene expression was analyzed by fluorescence production and immunohistochemical staining (Ad/CMV-TGF-β_1). Results Culture cells transducted by Ad/CMV-EGFP showed specific green fluorescence und er the fluoroscope and expression sustained for at least 4 weeks. When infe cted by Ad/CMV-TGF-β_1, approximally 30% of cultured cells were staind brown (+) with TGF-β_1 staining. Conclusion This study established the strategy of delivering a potentially therapeutic gene , TGF-β_1, by using an adenoviral vector to human degenerative lumbar interve rtebral disc cells.
文摘12月15日,知名营养美容品牌Lumì在上海老码头举办"Lumi into your life"新闻发布会,宣布正式签约人气影视花旦杨幂为2012年品牌形象代言人,首支由杨幂领衔主演的花漾唯美风Lumì胶原蛋白TVC在现场惊艳首映,揭开令万千女性如花绽放的胶原养美新’肌’密。近年来因出演古装穿越剧而人气飙升、荣升新"四小花旦"之首的杨幂,是继浙江卫视当红女主播朱丹之后。
文摘目的藏药五味甘露是藏药浴关键组方之一,目前五味甘露挥发油的成分尚不明确,其作用机理也不清楚,因此文章旨在分析其挥发油成分以及探明其作用机理。方法基于《四部医典》等藏医药古籍文献对五味甘露进行发酵;利用超临界CO_(2)提取挥发油;运用气相色谱质谱联用分析其挥发油成分;整合网络药理学预测其挥发性标志成分并分析其作用机理。结果从五味甘露挥发油中共鉴定出木香醇(10.51%)、柏木烯醇(5.51%)、γ-桉叶醇(2.36%)、桃拓酚(2.33%)、β-桉叶醇(2.28%)、榄香烯(1.59%)等176种化学成分;整合网络药理学预测五味甘露挥发性标志成分有3,7-二甲基-6-辛酸、γ-壬酸内酯、莪术双环烯酮、延胡索酮、γ-桉叶醇等,核心靶点有IL6、TNF、AKT1、IFNG、ALB、CCL2等,治疗类风湿性关节炎的生物学通路主要作用为side of membrane、lytic vacuole、lysosome等信号通路。结论本研究阐明了藏药五味甘露挥发油的化学成分,反映了中藏药“成分反映活性,活性指向功效”的思维;整合网络药理学预测藏药五味甘露挥发性标志成分并分析其作用机理,为临床前实验研究建立科学标准的质量评价体系提供参考依据及药理学理论基础。
