The by-catch fauna of the shrimp fishery includes a number of marine invertebrates that are discarded because they do not have commercial value.In order to try to add some value to these materials,we analyzed the chem...The by-catch fauna of the shrimp fishery includes a number of marine invertebrates that are discarded because they do not have commercial value.In order to try to add some value to these materials,we analyzed the chemical composition of the starfish Luidia senegalensis collected in the Brazilian coast as a consequence of the trawling fishery method.In order to access their chemical composition,we used a combination of solid phase extraction(SPE)followed by ultra-high performance liquid chromatography coupled to electrospray ionization ion trap tandem mass spectrometry(UPLC-ESI-IT-MSn).Luidia sene-galensis contains asterosaponins,which are sulphated glycosilated steroids,containing five and six sugar moieties,in addition to polyhydroxysteroids.This study helped us to support the presence of important and potentially bioactive compounds in invertebrates associated to the by-catch fauna of the shrimp fishery,using a fast and efficient method.展开更多
Luidia Forbes （Paxillosida： Luidiidae） are common soft bottom sea stars with 49 described species. Because of substan- tial morphological diversity, the taxonomy of the genus is complex and hasn＇t been resolved de...Luidia Forbes （Paxillosida： Luidiidae） are common soft bottom sea stars with 49 described species. Because of substan- tial morphological diversity, the taxonomy of the genus is complex and hasn＇t been resolved definitely. In order to resolve general taxonomic issues, and determine species boundaries and phylogenetic relationships within the genus Luidia, the sequences of tyro- chrome oxidase subunit I （COI） gene from 24 specimens of Luidia, belonging to eight taxa in Chinese waters, were studied. Three sequences of two species in genus Luidia from GenBank were used to analyze the phylogenetie relationships. The molecular phy- logeny exhibited three main clades, each with strong bootstrap support： Clade A including Luidia quinaria from the Sea of Japan; Clade B including seven nominal species （L. quinaria von Martens, L. yesoensis Goto, L. ehangi Liu, Liao and Li, L. orientalis Fisher, L. avicularia Fisher, L. longispina Sladen and L. hardwicki Gray） from Chinese waters; and Clade C including L. maculata Miiller ＆ Troschel from Chinese waters. Our molecular phylogeny results support the morphological Quinaria-Group and Alter- nata-Group assigned by D6derlein. Seven nominal species we sampled do not exhibit genetic distances that are large enough to rec- ognize them as separate species. Cryptic species may exist in ＇Luidia quinaria＇ from the Yellow Sea and the Sea of Japan. Meaning- ful morphological characters need further investigation in Luidia.展开更多
[目的]对砂海星(Luidia quinaria von Martens)提取残渣进行营养成分分析,为砂海星资源的综合开发利用提供理论依据。[方法]水分、灰分、粗脂肪、蛋白质含量的测定参照国家标准中规定的方法进行;还原糖、总糖、多糖的测定采用3,5-二硝...[目的]对砂海星(Luidia quinaria von Martens)提取残渣进行营养成分分析,为砂海星资源的综合开发利用提供理论依据。[方法]水分、灰分、粗脂肪、蛋白质含量的测定参照国家标准中规定的方法进行;还原糖、总糖、多糖的测定采用3,5-二硝基水杨酸比色法;氨基酸的测定采用高效液相色谱法,色氨酸的测定参照国家标准中规定的方法进行;脂肪酸的测定采用气—质联用法;化学元素Ca、Mg、Fe、Pb、Mn、Zn、Cu、Ni、Co、Cr的测定采用原子吸收光谱法,Hg的测定采用原子荧光光谱法。[结果]砂海星提取残渣中水分含量为1.75%,灰分含量为81.40%,粗脂肪含量为0.67%,蛋白质含量为11.99%,还原糖含量为0.20%,总糖含量为2.16%,多糖含量为1.96%,总氨基酸含量为13.69%,脂肪酸中不饱和脂肪酸含量较少,化学元素中Ca、Mg、Fe含量较高。[结论]砂海星提取残渣营养成分含量丰富,具有较高的开发利用价值。展开更多
基金supported by Funda-cao de Amparo a Pesquisa do Estado de Sao Paulo(FAPESP Grant 2012/19419-9 to W.V.and Grant 2011/23159-0 to M.M.P.T.)。
文摘The by-catch fauna of the shrimp fishery includes a number of marine invertebrates that are discarded because they do not have commercial value.In order to try to add some value to these materials,we analyzed the chemical composition of the starfish Luidia senegalensis collected in the Brazilian coast as a consequence of the trawling fishery method.In order to access their chemical composition,we used a combination of solid phase extraction(SPE)followed by ultra-high performance liquid chromatography coupled to electrospray ionization ion trap tandem mass spectrometry(UPLC-ESI-IT-MSn).Luidia sene-galensis contains asterosaponins,which are sulphated glycosilated steroids,containing five and six sugar moieties,in addition to polyhydroxysteroids.This study helped us to support the presence of important and potentially bioactive compounds in invertebrates associated to the by-catch fauna of the shrimp fishery,using a fast and efficient method.
基金supported by the Knowledge Innovation Program of CAS(KSCX2-YW-N-0807)the Ministry of Science and Technology of the People’s Republic of China(2006FY110500)IOCAS funding(2012IO060104)
文摘Luidia Forbes （Paxillosida： Luidiidae） are common soft bottom sea stars with 49 described species. Because of substan- tial morphological diversity, the taxonomy of the genus is complex and hasn＇t been resolved definitely. In order to resolve general taxonomic issues, and determine species boundaries and phylogenetic relationships within the genus Luidia, the sequences of tyro- chrome oxidase subunit I （COI） gene from 24 specimens of Luidia, belonging to eight taxa in Chinese waters, were studied. Three sequences of two species in genus Luidia from GenBank were used to analyze the phylogenetie relationships. The molecular phy- logeny exhibited three main clades, each with strong bootstrap support： Clade A including Luidia quinaria from the Sea of Japan; Clade B including seven nominal species （L. quinaria von Martens, L. yesoensis Goto, L. ehangi Liu, Liao and Li, L. orientalis Fisher, L. avicularia Fisher, L. longispina Sladen and L. hardwicki Gray） from Chinese waters; and Clade C including L. maculata Miiller ＆ Troschel from Chinese waters. Our molecular phylogeny results support the morphological Quinaria-Group and Alter- nata-Group assigned by D6derlein. Seven nominal species we sampled do not exhibit genetic distances that are large enough to rec- ognize them as separate species. Cryptic species may exist in ＇Luidia quinaria＇ from the Yellow Sea and the Sea of Japan. Meaning- ful morphological characters need further investigation in Luidia.
文摘[目的]对砂海星(Luidia quinaria von Martens)提取残渣进行营养成分分析,为砂海星资源的综合开发利用提供理论依据。[方法]水分、灰分、粗脂肪、蛋白质含量的测定参照国家标准中规定的方法进行;还原糖、总糖、多糖的测定采用3,5-二硝基水杨酸比色法;氨基酸的测定采用高效液相色谱法,色氨酸的测定参照国家标准中规定的方法进行;脂肪酸的测定采用气—质联用法;化学元素Ca、Mg、Fe、Pb、Mn、Zn、Cu、Ni、Co、Cr的测定采用原子吸收光谱法,Hg的测定采用原子荧光光谱法。[结果]砂海星提取残渣中水分含量为1.75%,灰分含量为81.40%,粗脂肪含量为0.67%,蛋白质含量为11.99%,还原糖含量为0.20%,总糖含量为2.16%,多糖含量为1.96%,总氨基酸含量为13.69%,脂肪酸中不饱和脂肪酸含量较少,化学元素中Ca、Mg、Fe含量较高。[结论]砂海星提取残渣营养成分含量丰富,具有较高的开发利用价值。
