The intact 741 hp polyhedrin gene of LsNPV was sequenced by Silver Sequencing System, and shares 90.6% and 97.0% nucleotide identity, 97.2% and 97. 6% amino acid identity with PfNPV and MdNPV polh genes respectively....The intact 741 hp polyhedrin gene of LsNPV was sequenced by Silver Sequencing System, and shares 90.6% and 97.0% nucleotide identity, 97.2% and 97. 6% amino acid identity with PfNPV and MdNPV polh genes respectively. The 14 hp conservative sequence with the core element GTAAG,is located in the 5'untranslated region of the gene. The polh gene was predicted to encodes a 246 amino sold residures with molecular weight of 29.0 kd, in which the number of acidic amino acids and alkaline amino acids was roughly equal resulting in almost no charges in polyhedrin protein molecule and hence occlusion body. It gives a valuable implication that ionic bonds as well as hydrophobic bonds and hydrogen bond may Play an important role in the crystallization or polyhedrin, by comparing amino acid variation of twenty-one polyhedrin. The comparison of promoter regions of polyhedrin gene and class Ⅲ gene shown that they are very similar, but also have differences in GC content.This could explain that both categories of gene are highly expressed, and polyhedrin genes are expressed more higher than class Ⅲ gene.展开更多
A 1500 bp DNA fragment of LsNPV genome was sequenced by silver sequencing system.A whole coding region of ORF1 and an incomplete ORF2 were found.ORF1 was 345 bp long and was predicted to encode a protein with 115 amin...A 1500 bp DNA fragment of LsNPV genome was sequenced by silver sequencing system.A whole coding region of ORF1 and an incomplete ORF2 were found.ORF1 was 345 bp long and was predicted to encode a protein with 115 amino acid residues,the 5'regulatory region of ORF1 contained early transcriptional consensus sequence such as AT/ACGTGT,CGTGC and a stem loop structure.On the 3'terminus of ORF1,a poly(A)tail signal was found.Incomplete ORF2 was 354 bp long and predicted to encode 118 amino acid residues,the 5'regulatory region of ORF2 contained two conserved late transcriptional motif ATAAG.Compared with all the genes from AcNPV,incomplete ORF2 showed 56.5%nucleotide sequence homology and 44.9%amino acid sequence homology with AcNPV PDV E66 gene.Two conserved ATAAG motif on 5'regulatory region of ORF2 was similar to those in AcNPV PDV E66 gene.ORF1 showed no high identity with AcNPV and other baculoviruses.展开更多
利用去血清方法建立 L s细胞凋亡的实验体系 ,证明 L s NPV p 35基因在 L s细胞中瞬时表达可以抑制L s细胞由于无血清造成的凋亡 .构建了在哺乳动物中高效表达 L s NPV p 35基因的载体 p Rc/ RSV- L p35 ,并导入Vero细胞瞬时表达 ,发现 ...利用去血清方法建立 L s细胞凋亡的实验体系 ,证明 L s NPV p 35基因在 L s细胞中瞬时表达可以抑制L s细胞由于无血清造成的凋亡 .构建了在哺乳动物中高效表达 L s NPV p 35基因的载体 p Rc/ RSV- L p35 ,并导入Vero细胞瞬时表达 ,发现 L s NPV p35基因产物也可以抑制 Vero细胞由于病毒感染造成的凋亡 .用含 L s NPVp35的质粒与 v Ac Anh(Ac NPV p35基因缺失病毒 ) DNA共转染 Sf- 9细胞 ,可以使 v Ac Anh DNA在 Sf- 9细胞中形成多角体 ,并且传代后多角体不扩增 ,表明 L s NPV p35基因与 Ac NPV p 35基因具有功能互补性 .展开更多
The occulusion derived virus envelope protein gene (odv e25) of Leucania separata nuclear polyhedrosis virus (LsNPV) was identified by the sequencing the pLsx3 fragment, and compared with other three baculovirus...The occulusion derived virus envelope protein gene (odv e25) of Leucania separata nuclear polyhedrosis virus (LsNPV) was identified by the sequencing the pLsx3 fragment, and compared with other three baculoviruses. The hydrophobility analysis showed that three odv e25 proteins share very high similarity.展开更多
文摘The intact 741 hp polyhedrin gene of LsNPV was sequenced by Silver Sequencing System, and shares 90.6% and 97.0% nucleotide identity, 97.2% and 97. 6% amino acid identity with PfNPV and MdNPV polh genes respectively. The 14 hp conservative sequence with the core element GTAAG,is located in the 5'untranslated region of the gene. The polh gene was predicted to encodes a 246 amino sold residures with molecular weight of 29.0 kd, in which the number of acidic amino acids and alkaline amino acids was roughly equal resulting in almost no charges in polyhedrin protein molecule and hence occlusion body. It gives a valuable implication that ionic bonds as well as hydrophobic bonds and hydrogen bond may Play an important role in the crystallization or polyhedrin, by comparing amino acid variation of twenty-one polyhedrin. The comparison of promoter regions of polyhedrin gene and class Ⅲ gene shown that they are very similar, but also have differences in GC content.This could explain that both categories of gene are highly expressed, and polyhedrin genes are expressed more higher than class Ⅲ gene.
文摘A 1500 bp DNA fragment of LsNPV genome was sequenced by silver sequencing system.A whole coding region of ORF1 and an incomplete ORF2 were found.ORF1 was 345 bp long and was predicted to encode a protein with 115 amino acid residues,the 5'regulatory region of ORF1 contained early transcriptional consensus sequence such as AT/ACGTGT,CGTGC and a stem loop structure.On the 3'terminus of ORF1,a poly(A)tail signal was found.Incomplete ORF2 was 354 bp long and predicted to encode 118 amino acid residues,the 5'regulatory region of ORF2 contained two conserved late transcriptional motif ATAAG.Compared with all the genes from AcNPV,incomplete ORF2 showed 56.5%nucleotide sequence homology and 44.9%amino acid sequence homology with AcNPV PDV E66 gene.Two conserved ATAAG motif on 5'regulatory region of ORF2 was similar to those in AcNPV PDV E66 gene.ORF1 showed no high identity with AcNPV and other baculoviruses.
文摘利用去血清方法建立 L s细胞凋亡的实验体系 ,证明 L s NPV p 35基因在 L s细胞中瞬时表达可以抑制L s细胞由于无血清造成的凋亡 .构建了在哺乳动物中高效表达 L s NPV p 35基因的载体 p Rc/ RSV- L p35 ,并导入Vero细胞瞬时表达 ,发现 L s NPV p35基因产物也可以抑制 Vero细胞由于病毒感染造成的凋亡 .用含 L s NPVp35的质粒与 v Ac Anh(Ac NPV p35基因缺失病毒 ) DNA共转染 Sf- 9细胞 ,可以使 v Ac Anh DNA在 Sf- 9细胞中形成多角体 ,并且传代后多角体不扩增 ,表明 L s NPV p35基因与 Ac NPV p 35基因具有功能互补性 .
文摘The occulusion derived virus envelope protein gene (odv e25) of Leucania separata nuclear polyhedrosis virus (LsNPV) was identified by the sequencing the pLsx3 fragment, and compared with other three baculoviruses. The hydrophobility analysis showed that three odv e25 proteins share very high similarity.
