Virus-induced gene silencing(VIGS)offers a rapid and high throughout technology platform for the analysis of gene function in plants.The barley stripe mosaic virus(BSMV)VIGS system was optimized in studies silencing p...Virus-induced gene silencing(VIGS)offers a rapid and high throughout technology platform for the analysis of gene function in plants.The barley stripe mosaic virus(BSMV)VIGS system was optimized in studies silencing phytoene desaturase expression in wheat,and demonstrated that infection with BSMV construct carrying a 412 bp fragment of TaRAR1 caused conversion of incompatible to compatible interactions to Lr24-mediated resistance in wheat TcLr24 and cultivar 5R615 harboring Lr24 whereas infection with a control construct had no effect on resistance or susceptibility.RT-PCR analysis showed that BSMV-induced gene silencing could be detected at mRNA levels.These studies indicated that TaRAR1 was a required component for Lr24-mediated race-specific resistance and the BSMV-VIGS was a powerful tool for dissecting the genetic pathways of disease resistance in hexaploid wheat.展开更多
This research was aimed to develop AFLP markers co-segregated with gene Lr24 and validate the using for marker assisted selection(MAS).An F2 population developed from the cross between the resistant line TcLr24 and th...This research was aimed to develop AFLP markers co-segregated with gene Lr24 and validate the using for marker assisted selection(MAS).An F2 population developed from the cross between the resistant line TcLr24 and the susceptible line Thatcher was tested for resistance to the Puccinia triticina races BGQQ and SHRT using for genetic analysis and molecular marker.A total of 224 AFLP primer combinations were used to test the resistant and susceptible parents,as well as the resistant bulk and the susceptible bulk.Four AFLP markers,P-AGA/M-CTT289 bp,P-AGC/M-CAC1ss bp,P-AGC/M-CAC162 bp,and P-ACG/M-CGC239 bp,were co-segregated with Lr24.The AFLP fragment from the primer combination P-ACG/M-CGC was cloned,sequenced and converted into a STS marker named as ASTS212.Thatcher backgrounded NILs and 115 varieties were examined by using this STS marker and the marker SCS13026oz developed by Gupta.5R615,5R616,IR13,and 1R17 were identified and validated to contain gene Lr24.The marker is dominant and may be useful in identification the resistance gene Lr24 in wheat and wheat breeding programs.展开更多
基金supported by the National Natural Science Foundation of China(30771391)
文摘Virus-induced gene silencing(VIGS)offers a rapid and high throughout technology platform for the analysis of gene function in plants.The barley stripe mosaic virus(BSMV)VIGS system was optimized in studies silencing phytoene desaturase expression in wheat,and demonstrated that infection with BSMV construct carrying a 412 bp fragment of TaRAR1 caused conversion of incompatible to compatible interactions to Lr24-mediated resistance in wheat TcLr24 and cultivar 5R615 harboring Lr24 whereas infection with a control construct had no effect on resistance or susceptibility.RT-PCR analysis showed that BSMV-induced gene silencing could be detected at mRNA levels.These studies indicated that TaRAR1 was a required component for Lr24-mediated race-specific resistance and the BSMV-VIGS was a powerful tool for dissecting the genetic pathways of disease resistance in hexaploid wheat.
基金the National Natural Science Foundation of China(30771391)the transgenic Major Projects,China(2009ZX08002-012B)the Specialized Research Fund for the Doctoral Program of Higher Education of China(20101302120005)for funding the research project
文摘This research was aimed to develop AFLP markers co-segregated with gene Lr24 and validate the using for marker assisted selection(MAS).An F2 population developed from the cross between the resistant line TcLr24 and the susceptible line Thatcher was tested for resistance to the Puccinia triticina races BGQQ and SHRT using for genetic analysis and molecular marker.A total of 224 AFLP primer combinations were used to test the resistant and susceptible parents,as well as the resistant bulk and the susceptible bulk.Four AFLP markers,P-AGA/M-CTT289 bp,P-AGC/M-CAC1ss bp,P-AGC/M-CAC162 bp,and P-ACG/M-CGC239 bp,were co-segregated with Lr24.The AFLP fragment from the primer combination P-ACG/M-CGC was cloned,sequenced and converted into a STS marker named as ASTS212.Thatcher backgrounded NILs and 115 varieties were examined by using this STS marker and the marker SCS13026oz developed by Gupta.5R615,5R616,IR13,and 1R17 were identified and validated to contain gene Lr24.The marker is dominant and may be useful in identification the resistance gene Lr24 in wheat and wheat breeding programs.
