目的:探究分枝杆菌脂蛋白LprO对分枝杆菌-巨噬细胞相互作用的影响。方法:使用在线网站分析耻垢分枝杆菌(Mycobacterium smegmatis,M.smeg)LprO蛋白的CD4^(+)T、CD8^(+)T以及细胞毒性T细胞(Cytotoxic T Lymphocytes,CTL)抗原表位数量,评...目的:探究分枝杆菌脂蛋白LprO对分枝杆菌-巨噬细胞相互作用的影响。方法:使用在线网站分析耻垢分枝杆菌(Mycobacterium smegmatis,M.smeg)LprO蛋白的CD4^(+)T、CD8^(+)T以及细胞毒性T细胞(Cytotoxic T Lymphocytes,CTL)抗原表位数量,评估LprO蛋白的免疫原性。构建lprO过表达的重组耻垢分枝杆菌M.smeg::pMV261-lprO,以转入空载质粒pMV261的M.smeg::pMV261菌株作为对照,分析过表达lprO对M.smeg菌株以及细菌-巨噬细胞互作的影响。结果:LprO蛋白中预测的CD4^(+)T、CD8^(+)T以及CD8^(+)CTL细胞表位数与Ag85A蛋白相当,具有较好的研究潜力。经实时荧光定量PCR(Quantitative real-time PCR,qRT-PCR)验证,M.smeg::pMV261-lprO菌株中,lprO表达量显著高于对照菌株,过表达菌株构建成功。lprO过表达不改变M.smeg菌落形态、细菌形态、细菌体外生长能力和巨噬细胞内生长能力。细菌侵染巨噬细胞Raw264.7,流式细胞技术检测显示,M.smeg::pMV261-lprO在细胞侵染前期能显著促进巨噬细胞凋亡。结论:分枝杆菌LprO蛋白可能具备与Ag85A蛋白相当的T细胞表位数,能在激起宿主的免疫反应中发挥较为重要的作用。在M.smeg中过表达LprO后能诱导侵染前期的巨噬细胞凋亡,参与细菌-宿主相互作用。综上,LprO蛋白或许有作为新型疫苗成分或药物靶标的潜力。展开更多
The analysis results made on drinking water from the rural areas of Kaolack,Diourbel and Fatick central region of Senegal showed the excess of fluoride and chloride ions.Depending on the area,the fluoride concentratio...The analysis results made on drinking water from the rural areas of Kaolack,Diourbel and Fatick central region of Senegal showed the excess of fluoride and chloride ions.Depending on the area,the fluoride concentration in the water can reach 3.5 mg/L.The values obtained are completely above of WHO(World Health Organization)limits.The objective of this study is to assess the performances of membrane filtration units powered by renewable energies for fluoride and salinity excess removal in remote village in Senegal.Many membrane filtration units have been installed in rural areas of regions such as Kaolack,Fatick and Diourbel.These membrane filtration units are equipped by the LPRO(Low Pressure Reverse Osmosis)membranes with 9 m^(2)of surface.A rejection rate of fluoride and chloride ions obtained is 99.33%and 95.25%respectively.The conversion rates are ranged from 45%to 65%.These results clearly show that membrane processes can be used in Africa,especially in isolated rural areas,with the combination of renewable energies.Currently,more than twenty membrane filtration units are installed in Senegal and provide drinking water of very good quality for populations living in rural areas.The prospect is to expand it on a larger scale,which is already underway,with the construction of a desalination plant in Dakar.展开更多
文摘The analysis results made on drinking water from the rural areas of Kaolack,Diourbel and Fatick central region of Senegal showed the excess of fluoride and chloride ions.Depending on the area,the fluoride concentration in the water can reach 3.5 mg/L.The values obtained are completely above of WHO(World Health Organization)limits.The objective of this study is to assess the performances of membrane filtration units powered by renewable energies for fluoride and salinity excess removal in remote village in Senegal.Many membrane filtration units have been installed in rural areas of regions such as Kaolack,Fatick and Diourbel.These membrane filtration units are equipped by the LPRO(Low Pressure Reverse Osmosis)membranes with 9 m^(2)of surface.A rejection rate of fluoride and chloride ions obtained is 99.33%and 95.25%respectively.The conversion rates are ranged from 45%to 65%.These results clearly show that membrane processes can be used in Africa,especially in isolated rural areas,with the combination of renewable energies.Currently,more than twenty membrane filtration units are installed in Senegal and provide drinking water of very good quality for populations living in rural areas.The prospect is to expand it on a larger scale,which is already underway,with the construction of a desalination plant in Dakar.
