The Loxostege sticticalis(Lepidoptera:Pyralidae)is a major migratory pest of agriculture and animal husbandry in Asia and Europe.Utilizing plant volatile organic compounds(pVOCs)as attractants for monitoring and contr...The Loxostege sticticalis(Lepidoptera:Pyralidae)is a major migratory pest of agriculture and animal husbandry in Asia and Europe.Utilizing plant volatile organic compounds(pVOCs)as attractants for monitoring and controlling pests is considered an environmentally friendly and effective method.However,limited knowledge exists regarding applying pVOCs to manage L.sticticalis.Here,volatile compounds released by Chenopodium album,Setaria viridis,and Medicago sativa,the three preferred oviposition plants for L.sticticalis females,were collected using dynamic headspace sampling techniques.A total of 55 distinct compounds were identified through gas chromatography-mass spectrometry(GC-MS),and 16 compounds in the concentration range from 0.001 to 100µgµL–1 elicited consistently enhanced electrophysiological responses in both male and female L.sticticalis.Subsequently,the attraction potential of four bioactive compounds-linalool,cis-anethole,trans-2-hexenal,and 1-octen-3-ol-were further confirmed by indoor behavioral bioassays.The blends of linalool,cis-anethole,trans-2-hexenal,and 1-octen-3-ol mixed at ratios of 5:1:5:10(formulation No.25)and 5:1:1:10(formulation No.21)were highly attractive to L.sticticalis adults.Field-trapping assays indicated that lure No.2 baited with formulation 21 demonstrated superior efficacy in field trapping.These findings suggest that pVOC-based attractants can be effectively employed for monitoring and mass trapping L.sticticalis adults,providing insights into the development of botanical attractants.展开更多
Exodsta civilis Rond. （ Diptera： Tachinidae） is an important parasitoid of Loxostege sticticalis L. （ Lepidoptera： Pyrelidae） and other lepidopterous pests. The biological characteristics of E. civilis were obse...Exodsta civilis Rond. （ Diptera： Tachinidae） is an important parasitoid of Loxostege sticticalis L. （ Lepidoptera： Pyrelidae） and other lepidopterous pests. The biological characteristics of E. civilis were observed in the laboratory with L. sticticalis as host. The adults emerged mainly during the daytime from 08：00 am to 10：00 am and 14：00 pm to 16：00 pm, and males emerged usually earlier than females, with the sex ratio of 1.26：1 （ 2/♂ ）. The average fecundity of female （number of eggs laid by one female） was 159.8 eggs, and the spawning duration lasted 16.6 d on average; the first spawning peak appeared on the fourth day after spawning. The longevity of adults was the longest at constant temperature of （23 ± 1 ） ℃. Supplement of sugary substances could significantly prolong the longevity of adults, especially feeding 20% hydromel could extend the longevity to 44 d. The emergence rate of E. civilis significantly decreased with an increase in storage time of L. sticticalis cocoon, but there was no further significant decrease after 110 d.展开更多
基金supported by the National Key R&D Program of China(2024YFE0113000)the Natural Science Foundation of Ningxia Province,China(2022AAC03241)the China Postdoctoral Science Foundation(2024M753573)。
文摘The Loxostege sticticalis(Lepidoptera:Pyralidae)is a major migratory pest of agriculture and animal husbandry in Asia and Europe.Utilizing plant volatile organic compounds(pVOCs)as attractants for monitoring and controlling pests is considered an environmentally friendly and effective method.However,limited knowledge exists regarding applying pVOCs to manage L.sticticalis.Here,volatile compounds released by Chenopodium album,Setaria viridis,and Medicago sativa,the three preferred oviposition plants for L.sticticalis females,were collected using dynamic headspace sampling techniques.A total of 55 distinct compounds were identified through gas chromatography-mass spectrometry(GC-MS),and 16 compounds in the concentration range from 0.001 to 100µgµL–1 elicited consistently enhanced electrophysiological responses in both male and female L.sticticalis.Subsequently,the attraction potential of four bioactive compounds-linalool,cis-anethole,trans-2-hexenal,and 1-octen-3-ol-were further confirmed by indoor behavioral bioassays.The blends of linalool,cis-anethole,trans-2-hexenal,and 1-octen-3-ol mixed at ratios of 5:1:5:10(formulation No.25)and 5:1:1:10(formulation No.21)were highly attractive to L.sticticalis adults.Field-trapping assays indicated that lure No.2 baited with formulation 21 demonstrated superior efficacy in field trapping.These findings suggest that pVOC-based attractants can be effectively employed for monitoring and mass trapping L.sticticalis adults,providing insights into the development of botanical attractants.
基金Supported by Special Fund for Agro-scientific Research in the Public Interest(201103002)"948"Project of Ministry of Agriculture(2011-G4)Applied Technology R&D Project of Inner Mongolia:"Research and Development of Key Technology for Green Grass Industry"and"Development of Key Pollution-free Control Technology for Ecological Rehabilitation Steppe"
文摘Exodsta civilis Rond. （ Diptera： Tachinidae） is an important parasitoid of Loxostege sticticalis L. （ Lepidoptera： Pyrelidae） and other lepidopterous pests. The biological characteristics of E. civilis were observed in the laboratory with L. sticticalis as host. The adults emerged mainly during the daytime from 08：00 am to 10：00 am and 14：00 pm to 16：00 pm, and males emerged usually earlier than females, with the sex ratio of 1.26：1 （ 2/♂ ）. The average fecundity of female （number of eggs laid by one female） was 159.8 eggs, and the spawning duration lasted 16.6 d on average; the first spawning peak appeared on the fourth day after spawning. The longevity of adults was the longest at constant temperature of （23 ± 1 ） ℃. Supplement of sugary substances could significantly prolong the longevity of adults, especially feeding 20% hydromel could extend the longevity to 44 d. The emergence rate of E. civilis significantly decreased with an increase in storage time of L. sticticalis cocoon, but there was no further significant decrease after 110 d.
