Crystal Growth in space is a crystalline process with long Period microgravity conditions.So far,on earth there are some facilities,such as DroP Tube,Drop Tower,Ballon,Sounding Rochets and Aircraft etc.They provide th...Crystal Growth in space is a crystalline process with long Period microgravity conditions.So far,on earth there are some facilities,such as DroP Tube,Drop Tower,Ballon,Sounding Rochets and Aircraft etc.They provide the microgravity conditions with the range of period from seconds to minutes.展开更多
In this study, acrylic acid （AA） and 4-azidoaniline were used to modify poly （N-isopropylacrylamide） （NIPAAm） in order to fabricate temperature-responsive surface for corneal epithelia cell adhesion and detachme...In this study, acrylic acid （AA） and 4-azidoaniline were used to modify poly （N-isopropylacrylamide） （NIPAAm） in order to fabricate temperature-responsive surface for corneal epithelia cell adhesion and detachment. First, NIPAAm was copolymerized with acrylic acid. Then, the copolymer was coupled with azidoaniline to synthesize AzPhPIA, derivative of p（NIPAAm-co-AA）, which possesses both thermo- and photo-sensitivities. Second, the synthesized copolymer was characterized by high performance liquid chromatography （HPLC）, Fourier transform infrared （FTIR） and a CHN analyzer. The thermo-sensitivity was characterized by temperature reducing experiment, contact angle measurement and low critical solution temperature （LCST） testing. Third, the derivatized copolymer was immobilized by photolithography on a polystyrene plate, and then the surface characterization of AzPhPIA-coated polystyrene plate （PSt） was measured by electron spectroscopy for chemical analysis （ESCA）. The thermo-sensitivity and cytocompatibility of the AzPhPIA-coated PSt were investigated by corneal epithelial cells culture. The results revealed that the AzPhPIA-coated PSt exhibited good cytocompatibility and cell detachability when temperature decreased.展开更多
文摘Crystal Growth in space is a crystalline process with long Period microgravity conditions.So far,on earth there are some facilities,such as DroP Tube,Drop Tower,Ballon,Sounding Rochets and Aircraft etc.They provide the microgravity conditions with the range of period from seconds to minutes.
基金supported by the National Natural Science Foundation of China (Nos.30970731 and 50832002)the Natural Science Foundation of Guangdong Province(No.9151063101000015)
文摘In this study, acrylic acid （AA） and 4-azidoaniline were used to modify poly （N-isopropylacrylamide） （NIPAAm） in order to fabricate temperature-responsive surface for corneal epithelia cell adhesion and detachment. First, NIPAAm was copolymerized with acrylic acid. Then, the copolymer was coupled with azidoaniline to synthesize AzPhPIA, derivative of p（NIPAAm-co-AA）, which possesses both thermo- and photo-sensitivities. Second, the synthesized copolymer was characterized by high performance liquid chromatography （HPLC）, Fourier transform infrared （FTIR） and a CHN analyzer. The thermo-sensitivity was characterized by temperature reducing experiment, contact angle measurement and low critical solution temperature （LCST） testing. Third, the derivatized copolymer was immobilized by photolithography on a polystyrene plate, and then the surface characterization of AzPhPIA-coated polystyrene plate （PSt） was measured by electron spectroscopy for chemical analysis （ESCA）. The thermo-sensitivity and cytocompatibility of the AzPhPIA-coated PSt were investigated by corneal epithelial cells culture. The results revealed that the AzPhPIA-coated PSt exhibited good cytocompatibility and cell detachability when temperature decreased.
