Zein/pectin/sodium caseinate composite nanoparticles(Z/P/S NPs)were prepared using reverse solvent precipitation and electrostatic adsorption to deliver flavonoids from loquat flower(FLF).The nanoparticles were spheri...Zein/pectin/sodium caseinate composite nanoparticles(Z/P/S NPs)were prepared using reverse solvent precipitation and electrostatic adsorption to deliver flavonoids from loquat flower(FLF).The nanoparticles were spherical in shape,with an average particle size of 132.95±0.45 nm and an entrapment rate of 89.10%±0.76%.Structural analysis suggested that FLF/Z/P/S NPs were mainly formed through hydrogen bonding,hydro-phobic and electrostatic interactions.The fluorescence intensity and secondary structure of the protein were significantly changed after modification,resulting in better stability and redispersibility of FLF/Z/P/S NPs.Meanwhile,FLF/Z/P/S NPs exhibited a significant sustained-release effect in simulated gastrointestinal fluids.The uptake of FLF/Z/P/S NPs by Caco-2 cells was significantly higher than that of FLF,and FLF/Z/P/S NPs had a better absorption effect and could inhibit the effect of extracellular excretion.This study constructed a stable nanoparticle delivery system that could effectively encapsulate and sustain FLF release while improving its absorption efficiency.It provides a method for the efficient utilization of loquat flower flavonoids and offers reference for stability research of other active substances.展开更多
基金supported by the foundation of Zhejiang Provincial Top Discipline of Biological Engineering(No.KF2021003).
文摘Zein/pectin/sodium caseinate composite nanoparticles(Z/P/S NPs)were prepared using reverse solvent precipitation and electrostatic adsorption to deliver flavonoids from loquat flower(FLF).The nanoparticles were spherical in shape,with an average particle size of 132.95±0.45 nm and an entrapment rate of 89.10%±0.76%.Structural analysis suggested that FLF/Z/P/S NPs were mainly formed through hydrogen bonding,hydro-phobic and electrostatic interactions.The fluorescence intensity and secondary structure of the protein were significantly changed after modification,resulting in better stability and redispersibility of FLF/Z/P/S NPs.Meanwhile,FLF/Z/P/S NPs exhibited a significant sustained-release effect in simulated gastrointestinal fluids.The uptake of FLF/Z/P/S NPs by Caco-2 cells was significantly higher than that of FLF,and FLF/Z/P/S NPs had a better absorption effect and could inhibit the effect of extracellular excretion.This study constructed a stable nanoparticle delivery system that could effectively encapsulate and sustain FLF release while improving its absorption efficiency.It provides a method for the efficient utilization of loquat flower flavonoids and offers reference for stability research of other active substances.
