HPLC fingerprinting and quantification of gentiopicroside（GPS） and loganic acid（LA） in Gentianae Macrophyllae Radix（GMR） crude drugs were developed in this study.The samples were separated on Zorbax SB-C_（18） ...HPLC fingerprinting and quantification of gentiopicroside（GPS） and loganic acid（LA） in Gentianae Macrophyllae Radix（GMR） crude drugs were developed in this study.The samples were separated on Zorbax SB-C_（18） column（250 mm×4.6 mm, 5μm） with a linear gradient of acetonitrile and 0.04%phosphoric acid.The HPLC flow rate was 1.0 mL/min and a UV absorption was measured at 230 nm.An orthogonal L9（3^4） test was applied for the optimization of sample extraction conditions,and an aliquot of GMR sample（g） was extracted with 15-fold of 50%ethanol（mL） for 30 min by sonication.Quantitative analysis showed that the content of GPS（14.05 mg/g-74.61 mg/g） in all samples was obviously higher than that of LA（1.13 mg/g-40.46 mg/g）. Based on the content ratio of GPS over LA（1.8-11.4）,samples originated from Gentiana macrophylla（with content ratio of GPS over LA≤4.3） could be distinguished from those from G.dahurica and G.dahurica var.gracilipes（with content ratio of GPS over LA≥4.8）.The principle components analysis of the HPLC fingerprints showed that samples originated from G.macrophylla and G.dahurica（including G.dahurica var.gracilipes） could be divided into two groups.This established HPLC-DAD method could be efficiently used for the species identification and quality control of GMR crude drugs.展开更多
[Objectives] In order to develop Triplostegia glandulifera Wall.ex DC. into foromula granules, a high performance liquid chromatography (HPLC) determination method and a TLC identification method for loganic acid in d...[Objectives] In order to develop Triplostegia glandulifera Wall.ex DC. into foromula granules, a high performance liquid chromatography (HPLC) determination method and a TLC identification method for loganic acid in decoction pieces, standard decoction and formula granules of T. glandulifera were established, and the quantity value transfer relationship of T. glandulifera pieces-standard decoction-formula granules was investigated.[Methods] An Agilent 1260 II high performance liquid chromatograph and a Waters Symmetry C_(18) column (4.6 mm×250 mm, 5 μm) were used to perform gradient elution with acetonitrile-0.1%phosphoric acid solution as the mobile phase at a column temperature of 30 ℃ and a flow rate of 1 mL/min, and the detection wavelength was 240 nm. The TLC identification method of T. glandulifera was established using ethyl acetate-methanol-water (10 : 2 : 1) as the developer, and examination was carried out under a UV lamp (254 nm). The quantity value transfer law was analyzed by using the extract yield, the content of loganic acid and TLC chromatograms as the main evaluation indexes.[Results] The method for the determination and identification of loganic acid is stable, reproducible and reliable. The average yield, average loganic acid content and average loganic acid content transfer rate of 3 batches of T. glandulifera formula granules were, respectively, 18.7%, 41.1 mg/g and 43.1%, each of which was within corresponding range of mean±3SD of the 15 batches of standard decoction.[Conclusions] The content determination and TLC identification methods of loganic acid can be used to evaluate the quality of T. glandulifera formula granules. This study provides data basis for further research on T. glandulifera formula granules, and promotes the modernization of medicines for ethnic minorities.展开更多
mmJ这个骷髅头，不知不觉已经走过了10个年头。
07年下半年，正是本间正章发动mastermind JAPAN 10周年企画的大日子，「THANKYOU」FW系列更说明是贯穿整个10周年企画的别注系列。顾名思义，mmJ除了mainline的10周年外，本间更联同一...mmJ这个骷髅头，不知不觉已经走过了10个年头。
07年下半年，正是本间正章发动mastermind JAPAN 10周年企画的大日子，「THANKYOU」FW系列更说明是贯穿整个10周年企画的别注系列。顾名思义，mmJ除了mainline的10周年外，本间更联同一众好友品牌推出10周年限定别注，中村世纪的visvim是就是其合作的重头之一。展开更多
基金Research Program of Science Technology of Traditional Chinese Medicine(TCM) Sponsored by the State Administration of TCM of China(Grant No.04-05ZL01)
文摘HPLC fingerprinting and quantification of gentiopicroside（GPS） and loganic acid（LA） in Gentianae Macrophyllae Radix（GMR） crude drugs were developed in this study.The samples were separated on Zorbax SB-C_（18） column（250 mm×4.6 mm, 5μm） with a linear gradient of acetonitrile and 0.04%phosphoric acid.The HPLC flow rate was 1.0 mL/min and a UV absorption was measured at 230 nm.An orthogonal L9（3^4） test was applied for the optimization of sample extraction conditions,and an aliquot of GMR sample（g） was extracted with 15-fold of 50%ethanol（mL） for 30 min by sonication.Quantitative analysis showed that the content of GPS（14.05 mg/g-74.61 mg/g） in all samples was obviously higher than that of LA（1.13 mg/g-40.46 mg/g）. Based on the content ratio of GPS over LA（1.8-11.4）,samples originated from Gentiana macrophylla（with content ratio of GPS over LA≤4.3） could be distinguished from those from G.dahurica and G.dahurica var.gracilipes（with content ratio of GPS over LA≥4.8）.The principle components analysis of the HPLC fingerprints showed that samples originated from G.macrophylla and G.dahurica（including G.dahurica var.gracilipes） could be divided into two groups.This established HPLC-DAD method could be efficiently used for the species identification and quality control of GMR crude drugs.
基金Supported by Yunnan Provincial Science and Technology Major Project(202102AA310027).
文摘[Objectives] In order to develop Triplostegia glandulifera Wall.ex DC. into foromula granules, a high performance liquid chromatography (HPLC) determination method and a TLC identification method for loganic acid in decoction pieces, standard decoction and formula granules of T. glandulifera were established, and the quantity value transfer relationship of T. glandulifera pieces-standard decoction-formula granules was investigated.[Methods] An Agilent 1260 II high performance liquid chromatograph and a Waters Symmetry C_(18) column (4.6 mm×250 mm, 5 μm) were used to perform gradient elution with acetonitrile-0.1%phosphoric acid solution as the mobile phase at a column temperature of 30 ℃ and a flow rate of 1 mL/min, and the detection wavelength was 240 nm. The TLC identification method of T. glandulifera was established using ethyl acetate-methanol-water (10 : 2 : 1) as the developer, and examination was carried out under a UV lamp (254 nm). The quantity value transfer law was analyzed by using the extract yield, the content of loganic acid and TLC chromatograms as the main evaluation indexes.[Results] The method for the determination and identification of loganic acid is stable, reproducible and reliable. The average yield, average loganic acid content and average loganic acid content transfer rate of 3 batches of T. glandulifera formula granules were, respectively, 18.7%, 41.1 mg/g and 43.1%, each of which was within corresponding range of mean±3SD of the 15 batches of standard decoction.[Conclusions] The content determination and TLC identification methods of loganic acid can be used to evaluate the quality of T. glandulifera formula granules. This study provides data basis for further research on T. glandulifera formula granules, and promotes the modernization of medicines for ethnic minorities.
文摘mmJ这个骷髅头，不知不觉已经走过了10个年头。
07年下半年，正是本间正章发动mastermind JAPAN 10周年企画的大日子，「THANKYOU」FW系列更说明是贯穿整个10周年企画的别注系列。顾名思义，mmJ除了mainline的10周年外，本间更联同一众好友品牌推出10周年限定别注，中村世纪的visvim是就是其合作的重头之一。
