[Objective] This study aimed to investigate the effect of potyamine priming on physiological and biochemical variations of Lolium perenne embryos and seed germination. [Method] With annual Lolium perenne （Diamond T a...[Objective] This study aimed to investigate the effect of potyamine priming on physiological and biochemical variations of Lolium perenne embryos and seed germination. [Method] With annual Lolium perenne （Diamond T and Grazing-8000） as experimental materials, after priming with 0.5 mmol/L putrescine （Put）, spermidine （Spd） and spermine （Spm） for 24 h and chilling imbibition at 5 ℃ for 12, 24, 36 and 48 h, the effect of Put, Spd and Spm priming on chilling tolerance and germination ability of annual Lolium perenne seeds during imbibition was investigated. [Result] Put, Spd and Spm priming improved the activities of peroxidase （POD）, catalase （CAT）, superoxide dismutase （SOD） and ascorbate peroxidase （APX） and content of soluble protein content under low temperature stress, significantly in-creased the germination rate, and shortened the average germination duration. After chilling imbibition for 48 h, compared with the control, the average germination rate of annual Lolium perenne seeds was improved by 15.5% and 12.0% after Put, Spd and Spm priming, and the average germination duration was shortened by 1.21 and 1.14 d. During seed imbibition, the chilling tolerance of Grazing-8000 was stronger than that of Diamond T. Overall, Put, Spd and Spm treatment could increase the chilling tolerance of annual Lolium perenne seeds during imbibition, and improve the germination ability of seeds under low temperature stress. [Conclusion] Results of this study provided theoretical basis for the application of seed priming technology in the production of annual ryegrass.展开更多
This study was conducted to investigate the combined effects of elevated CO2 levels and cadmium (Cd) on the root morphological traits and Cd accumulation in Lolium multiflorum Lam.and Lolium perenne L.exposed to two C...This study was conducted to investigate the combined effects of elevated CO2 levels and cadmium (Cd) on the root morphological traits and Cd accumulation in Lolium multiflorum Lam.and Lolium perenne L.exposed to two CO2 levels (360 and 1000 μl/L) and three Cd levels (0,4,and 16 mg/L) under hydroponic conditions.The results show that elevated levels of CO2 increased shoot biomass more,compared to root biomass,but decreased Cd concentrations in all plant tissues.Cd exposure caused toxicity to both Lolium species,as shown by the restrictions of the root morphological parameters including root length,surface area,volume,and tip numbers.These parameters were significantly higher under elevated levels of CO2 than under ambient CO2,especially for the number of fine roots.The increases in magnitudes of those parameters triggered by elevated levels of CO2 under Cd stress were more than those under non-Cd stress,suggesting an ameliorated Cd stress under elevated levels of CO2.The total Cd uptake per pot,calculated on the basis of biomass,was significantly greater under elevated levels of CO2 than under ambient CO2.Ameliorated Cd toxicity,decreased Cd concentration,and altered root morphological traits in both Lolium species under elevated levels of CO2 may have implications in food safety and phytoremediation.展开更多
基金Supported by Agricultural Research Project of Guizhou Province([2010]No.3045)~~
文摘[Objective] This study aimed to investigate the effect of potyamine priming on physiological and biochemical variations of Lolium perenne embryos and seed germination. [Method] With annual Lolium perenne （Diamond T and Grazing-8000） as experimental materials, after priming with 0.5 mmol/L putrescine （Put）, spermidine （Spd） and spermine （Spm） for 24 h and chilling imbibition at 5 ℃ for 12, 24, 36 and 48 h, the effect of Put, Spd and Spm priming on chilling tolerance and germination ability of annual Lolium perenne seeds during imbibition was investigated. [Result] Put, Spd and Spm priming improved the activities of peroxidase （POD）, catalase （CAT）, superoxide dismutase （SOD） and ascorbate peroxidase （APX） and content of soluble protein content under low temperature stress, significantly in-creased the germination rate, and shortened the average germination duration. After chilling imbibition for 48 h, compared with the control, the average germination rate of annual Lolium perenne seeds was improved by 15.5% and 12.0% after Put, Spd and Spm priming, and the average germination duration was shortened by 1.21 and 1.14 d. During seed imbibition, the chilling tolerance of Grazing-8000 was stronger than that of Diamond T. Overall, Put, Spd and Spm treatment could increase the chilling tolerance of annual Lolium perenne seeds during imbibition, and improve the germination ability of seeds under low temperature stress. [Conclusion] Results of this study provided theoretical basis for the application of seed priming technology in the production of annual ryegrass.
基金Project supported by the Central Public Research Institute Basic Fund for Research and Development (2008-jxh-1),Agro-environmental Protection Institute,Ministry of Agriculture,China
文摘This study was conducted to investigate the combined effects of elevated CO2 levels and cadmium (Cd) on the root morphological traits and Cd accumulation in Lolium multiflorum Lam.and Lolium perenne L.exposed to two CO2 levels (360 and 1000 μl/L) and three Cd levels (0,4,and 16 mg/L) under hydroponic conditions.The results show that elevated levels of CO2 increased shoot biomass more,compared to root biomass,but decreased Cd concentrations in all plant tissues.Cd exposure caused toxicity to both Lolium species,as shown by the restrictions of the root morphological parameters including root length,surface area,volume,and tip numbers.These parameters were significantly higher under elevated levels of CO2 than under ambient CO2,especially for the number of fine roots.The increases in magnitudes of those parameters triggered by elevated levels of CO2 under Cd stress were more than those under non-Cd stress,suggesting an ameliorated Cd stress under elevated levels of CO2.The total Cd uptake per pot,calculated on the basis of biomass,was significantly greater under elevated levels of CO2 than under ambient CO2.Ameliorated Cd toxicity,decreased Cd concentration,and altered root morphological traits in both Lolium species under elevated levels of CO2 may have implications in food safety and phytoremediation.
