Single-cell oils(SCO)produced by oleaginous yeast hold promise as a sustainable alternative for producing nutritionally and pharmaceutically valuable lipids.However,the accumulation of oils varies substantially betwee...Single-cell oils(SCO)produced by oleaginous yeast hold promise as a sustainable alternative for producing nutritionally and pharmaceutically valuable lipids.However,the accumulation of oils varies substantially between yeast spp.Consequently,identifying well-suited producers with a high innate capacity for lipids biosynthesis is paramount.Equally important is optimizing culturing and processing conditions to realize the total lipids production potential of selected strains.The marine Rhodotorula mucilaginosa and Lodderomyces elongisporus yeast were investigated to explore their potential for polyunsaturated fatty acids(PUFAs)production on high glucose media(HGM)using two-stage culture mode.Both strains accumulated>20%(w/w)of their dry cell weight as lipids when grown on HGM using a two-stage culture system.Both yeast isolates exhibited a maximal lipid/biomass coefficient(YL/X)of 0.58-0.66 mg/mg at 7°C and 0.49-0.53 mg/mg at 26°C when grown on 8%glucose and produced monounsaturated and PUFAs similar to that of Menhaden and Salmon marine oils.For the first time,significant amounts of Eicosapentaenoic acid(19%)and Eicosadienoic acid(19.6%)were produced by L.elongisporus and R.mucilaginosa,respectively.Thus,the SCO derived from these wild strains possesses significant potential as a substitute source for the industrial-scale production of long-chain PUFAs,making them a promising contender in the market.展开更多
基金Open access funding provided by The Science,Technology&Innovation Funding Authority(STDF)in cooperation with The Egyptian Knowledge Bank(EKB)We acknowledge the partial financial support from the Industrial Biotechnology,Genetic Engineering and Biotechnology Research Institute(GEBRI),University of Sadat City,Egypt.
文摘Single-cell oils(SCO)produced by oleaginous yeast hold promise as a sustainable alternative for producing nutritionally and pharmaceutically valuable lipids.However,the accumulation of oils varies substantially between yeast spp.Consequently,identifying well-suited producers with a high innate capacity for lipids biosynthesis is paramount.Equally important is optimizing culturing and processing conditions to realize the total lipids production potential of selected strains.The marine Rhodotorula mucilaginosa and Lodderomyces elongisporus yeast were investigated to explore their potential for polyunsaturated fatty acids(PUFAs)production on high glucose media(HGM)using two-stage culture mode.Both strains accumulated>20%(w/w)of their dry cell weight as lipids when grown on HGM using a two-stage culture system.Both yeast isolates exhibited a maximal lipid/biomass coefficient(YL/X)of 0.58-0.66 mg/mg at 7°C and 0.49-0.53 mg/mg at 26°C when grown on 8%glucose and produced monounsaturated and PUFAs similar to that of Menhaden and Salmon marine oils.For the first time,significant amounts of Eicosapentaenoic acid(19%)and Eicosadienoic acid(19.6%)were produced by L.elongisporus and R.mucilaginosa,respectively.Thus,the SCO derived from these wild strains possesses significant potential as a substitute source for the industrial-scale production of long-chain PUFAs,making them a promising contender in the market.
