Axial chain rockbursts(ACRs)repeatedly occur in deep tunnels during drilling and blasting methodology(D&B)within locked-in stress zones,severely hindering construction progress.In extremely cases,ACRs can persist ...Axial chain rockbursts(ACRs)repeatedly occur in deep tunnels during drilling and blasting methodology(D&B)within locked-in stress zones,severely hindering construction progress.In extremely cases,ACRs can persist for 7−10 d and affect areas exceeding 20 m along tunnel axis.Through integrated geological investigations and microseismic(MS)monitoring,the geological characteristics,MS activity patterns,and formation mechanisms of ACRs were analyzed.In tectonically active regions,locked-in stress zones arise from interactions between multiple structural planes.Blasting dynamic disturbances during tunnel excavation in these zones trigger early slippage along structural planes and fractures in the surrounding rock,with MS events developing ahead of the working face.High-energy MS events dominate during the development and occurrence stages of ACRs,extending 20−30 m(3−4 tunnel diameters)ahead of the working face.Following the ACRs,low-energy MS events primarily occur behind the working face.Tensile fracturing is the predominant failure mode during ACRs.Shear and mixed fractures primarily occur within the ACRs zone during the intra-ACR phase.Monitoring MS event locations ahead of the working face provides a reliable approach for prewarning potential ACR-prone zones.展开更多
Rocks are composed of mineral particles and micropores between mineral which has a great influence on the mechanical properties of rocks. In this paper, based on the theory of locked-in stress developed by academician...Rocks are composed of mineral particles and micropores between mineral which has a great influence on the mechanical properties of rocks. In this paper, based on the theory of locked-in stress developed by academician Chen Zongji, the locked-in stress problem in underground rock is simulated by the thermal expansion of hard rubber particles. The pore inclusion in rock is assumed to be uniformly distributed spherical cavities. Using the thermal stress theory, the stress of rock with a spherical pore inclusion is equivalent to the thermal stress generated by the spherical hard rubber inclusion. The elastic theory formula of the temperature increment and the equivalent pore pressure of the spherical hard rubber inclusion is derived. The numerical simulation of the rock mass model with a spherical hard rubber inclusion is carried out and compared to the theoretical calculation results<span lang="EN-US" style="font-family:;" minion="" pro="" capt",serif;font-size:10pt;mso-fareast-font-family:宋体;mso-bidi-font-family:"times="" new="" roman";mso-ansi-language:en-us;mso-fareast-language:zh-cn;mso-bidi-language:ar-sa;mso-bidi-font-weight:bold;"="">;</span><span lang="EN-US" style="font-family:;" minion="" pro="" capt",serif;font-size:10pt;mso-fareast-font-family:宋体;mso-bidi-font-family:"times="" new="" roman";mso-ansi-language:en-us;mso-fareast-language:en-us;mso-bidi-language:ar-sa;mso-bidi-font-weight:bold;"=""> the results show that they are consistent. The method proposed by this paper for simulating stress distribution in rock by thermal stress is reasonable and feasible</span><span lang="EN-US" style="font-family:;" minion="" pro="" capt",serif;font-size:10pt;mso-fareast-font-family:宋体;mso-bidi-font-family:"times="" new="" roman";mso-ansi-language:en-us;mso-fareast-language:zh-cn;mso-bidi-language:ar-sa;mso-bidi-font-weight:bold;"="">;</span><span lang="EN-US" style="font-family:;" minion="" pro="" capt",serif;font-size:10pt;mso-fareast-font-family:宋体;mso-bidi-font-family:"times="" new="" roman";mso-ansi-language:en-us;mso-fareast-language:en-us;mso-bidi-language:ar-sa;mso-bidi-font-weight:bold;"=""> it has a positive meaning for further study of mechanic phenomenon of rock with micropore inclusion.</span>展开更多
In light of the pressing global challenges of climate change,declining crop resilience,and hidden hunger,it is imperative to overcome the limitations of conventional crop breeding to enhance both the nutritional quali...In light of the pressing global challenges of climate change,declining crop resilience,and hidden hunger,it is imperative to overcome the limitations of conventional crop breeding to enhance both the nutritional quality and stress tolerance of crops.Synthetic metabolic engineering presents innovative strategies for the precision modification and de novo design of metabolic pathways.This approach generally encompasses three essential steps:identifying key metabolites through metabolomics,integrating multi-omics technologies to investigate the synthesis and regulation of these metabolites,and utilizing gene editing or de novo design to modify crop metabolic pathways associated with desirable agronomic traits.This review underscores the vital role of plant metabolite diversity in enhancing crop nutritional quality and stress resilience.Integrated multi-omics analyses facilitate the metabolic engineering by identifying key genes,transporters,and transcription factors that regulate metabolite biosynthesis.Precision modification strategies employ genome editing tools to reprogram endogenous metabolic networks,while de novo design reconstructs metabolic pathways through the introduction of exogenous biological elements—thereby both approaches enable the targeted enhancement of desired traits.These strategies have been effectively implemented in major food crops.However,simultaneously enhancing nutritional quality and stress resilience remains challenging due to inherent trade-offs and resource competition in distinct metabolic pathways within plants.Future research should integrate AI-driven predictive models with multi-omics datasets to decipher dynamic metabolic homeostasis and engineer climate-smart crops that maximize yield while preserving quality and environmental adaptability.展开更多
Background Environmental hypoxia is a common phenomenon in aquaculture,which causes gill tissue injury in fish.Glutathione(GSH)is a vital antioxidant in animal tissues,and its levels decrease under hypoxic conditions....Background Environmental hypoxia is a common phenomenon in aquaculture,which causes gill tissue injury in fish.Glutathione(GSH)is a vital antioxidant in animal tissues,and its levels decrease under hypoxic conditions.However,the effects of glutathione on fish under hypoxic stress remain poorly understood.This study aimed to investigate the impact of glutathione on gill tissue damage in fish under hypoxic stress and explore the underlying mechanisms.Methods Six experimental diets with varying glutathione concentrations.The actual glutathione levels in these diets,measured by high-performance liquid chromatography,were 0.00,145.95,291.90,437.85,583.80,and 729.75 mg/kg,respectively.Fish were fed these diets for 70 d,after which a 96-h hypoxic stress experiment was conducted.The experiment was set up with normoxic and hypoxic groups,in which the dissolved oxygen in the group was 6 mg/L,and that in the group was 1 mg/L.Results This research revealed that glutathione could enhance the growth performance and antioxidant capability of juvenile grass carp while mitigating the structural damage to gill tissues induced by hypoxia stress.Mechanistic investigations further indicated that glutathione mitigated hypoxia-induced oxidative injury in gill tissues and improved their antioxidant capacity.In addition,glutathione attenuated gill apoptosis induced by hypoxia stress.Glutathione also inhibited the initiation,nucleation,elongation,and degradation phases of autophagy,thereby attenuating hypoxia-induced gill autophagy.Moreover,glutathione was found to alleviate hypoxia-induced endoplasmic reticulum stress(ERS)in gills,a response potentially linked to the suppression of PERK,IRE1,and ATF6 signaling pathways.Finally,based on the ROS and PC contents in gill tissue,the optimum glutathione supplementation levels for juvenile grass carp under hypoxia stress were 437.10 and 495.00 mg/kg,respectively.Conclusions In conclusion,our experimental results demonstrated the effectiveness of glutathione in alleviating gill tissue damage caused by hypoxic stress.This study confirms the feasibility and effectiveness of dietary glutathione addition to alleviate hypoxic stress in fish.展开更多
Rice production is increasingly challenged by flooding stress because of global warming and rising sea levels.As the world’s most important staple crop,rice is highly vulnerable to anaerobic and submergence condition...Rice production is increasingly challenged by flooding stress because of global warming and rising sea levels.As the world’s most important staple crop,rice is highly vulnerable to anaerobic and submergence conditions that occur during flooding,particularly at the germination and vegetative stages.Anaerobic environments hinder seedling establishment during germination,while prolonged submergence during the vegetative stage impairs growth,ultimately reducing yield and grain quality.These stresses,driven by extended inundation,trigger a cascade of detrimental physiological responses and represent a major barrier to stable rice production and global food security.In this review,we examine the effects of flooding on rice growth at both the germination and vegetative stages.We further summarize recent advances in the identification of flooding-tolerant germplasm,QTL mapping,genome-wide association study,transcriptomic and proteomic analyses,and other molecular studies.Subsequently,we highlight potential cultivation and regulatory strategies,including genetic,morphological,physiological,and endogenous hormone-related approaches,aimed at enhancing tolerance to anaerobic and submergence stress.Together,these approaches underscore the promise of integrating molecular insights with agronomic practices to mitigate flooding damage and support sustainable rice production.展开更多
Asymmetric deformation and failure of surrounding rock are frequently observed in mountain tunnels and deep mining roadways,yet the underlying mechanisms remain poorly understood.To investigate asymmetric failure in r...Asymmetric deformation and failure of surrounding rock are frequently observed in mountain tunnels and deep mining roadways,yet the underlying mechanisms remain poorly understood.To investigate asymmetric failure in roadways adjacent to fault structures and mining panels,this study adopts an integrated approach combining theoretical derivation,numerical simulation,and field application,with particular emphasis on the second invariant of the stress deviator(J_(2) )in the surrounding rock.Based on the stress solution for a circular opening,an analytical expression for J_(2)(distortion energy)is derived by considering the reorientation of principal stresses.The study demonstrates that both the increase and reorientation of principal stresses induced by fault–mining interaction jointly govern the spatial distribution of J_(2) and the resulting asymmetric failure behavior.Specifically,the principal stress rotation angle determines the location of J_(2) concentration,whereas the principal stress ratio controls its magnitude.To mitigate asymmetric failure,it is recommended to optimize the J_(2) state through adjustments in roadway size,geometry,and support systems,while simultaneously controlling the asymmetric concentration of stress deviator to enhance roadway stability.This study systematically elucidates the chain mechanism of asymmetric surrounding rock failure driven by principal stress,and further proposes a rational asymmetric joint control strategy,providing theoretical guidance for similar underground engineering conditions.展开更多
Protein aggregates,mitochondrial import stress and neurodegenerative disorders:A salient hallmark of several neurodegenerative diseases,including Parkinson’s disease,is the abundance of protein aggregates(Goiran et a...Protein aggregates,mitochondrial import stress and neurodegenerative disorders:A salient hallmark of several neurodegenerative diseases,including Parkinson’s disease,is the abundance of protein aggregates(Goiran et al.,2022).This molecular event is believed to lead to activation of stress pathways ultimately resulting in cellular dysfunction(Eldeeb et al.,2022).Accordingly,many lines of research investigations focused on dampening the formation of protein aggregates or augmenting the clearance of protein aggregates as a potential therapeutic strategy to counteract the progression of neurodegenerative diseases,albeit with little success(Costa-Mattioli and Walter,2020).Cell stress cues such as the accumulation of protein aggregates lead to the activation of stress response pathways that aid cells in responding to the damage.Despite the notion that the transient activation of these pathways helps cells cope with stressors,persistent activation can induce unwanted apoptosis of cells and reduce overall tissue strength as well as lead to an accumulation of aggregation-prone proteins(Hetz and Papa,2018).Mutations in proteins involved in stress signaling termination can cause conditions like ataxia and early-onset dementia(Conroy et al.,2014).Therefore,it is crucial for stress response signaling to be turned off once conditions have improved.Nevertheless,the mechanisms by which cells silence these signals are still elusive.展开更多
Excavation causes stress redistribution and affects the stress path during the shearing process of rock.The shear strength of rock varies under different stress paths,and the presence of defects reduces the shear stre...Excavation causes stress redistribution and affects the stress path during the shearing process of rock.The shear strength of rock varies under different stress paths,and the presence of defects reduces the shear strength.To further investigate this phenomenon,this study investigates the shear behaviour of rocks with different shear surface integrities under the influenceof different stress paths through laboratory tests and numerical simulations.The results indicate that the shear strength depends on the stress path and a decrease in the shear surface integrity reduces the degree of dependence.The cohesion and friction angle of the Mohr‒Coulomb criterion decrease with weakening of the shear surface integrity.For different stress paths,the direct shear strength is always greater than that of other shear stress paths.The pattern of changes in the acoustic emission count and cumulative count indirectly reflectsthe above findings.Numerical simulations further indicate that the different principal stress states and normal suppression effects during the shearing process lead to changes in the factors of crack propagation,resulting in different mechanical behaviours under various stress paths.For rocks with different integrity levels,the main reason for the different path dependences of shear strength is that the size of the area affected by shear is different.Shear failure will concentrate on the shear plane when the normal inhibition effect is greater.This study explores the mechanism of rock shear behaviour,providing a theoretical basis for establishing more accurate constitutive models and strength criteria.展开更多
Background Hypoxia is a pervasive challenge in aquaculture that poses a significant threat to aquatic organisms.Since fish cannot synthesize vitamin A endogenously,it must be supplied through diet,and it plays a vital...Background Hypoxia is a pervasive challenge in aquaculture that poses a significant threat to aquatic organisms.Since fish cannot synthesize vitamin A endogenously,it must be supplied through diet,and it plays a vital role in supporting fish stress resistance.This study aimed to investigate the protective effects of VA on the gills of adult grass carp(Ctenopharyngodon idella)against hypoxia and to elucidate the underlying mechanisms.Methods Six experimental diets with graded VA levels(375,862,1,614,2,099,2,786,and 3,118 IU/kg)were fed to grass carp(initial weight:726±1.2 g)for 60 d.After the trial,24 fish per treatment were selected,divided equally into normoxic and hypoxic groups,fasted for 24 h,and then subjected to a 96-h acute hypoxic challenge.Results The results demonstrated that VA supplementation mitigated hypoxia-induced damage in gill tissue,as evidenced by histological examination.Furthermore,VA alleviated oxidative stress,as indicated by reduced levels of lactate(LD),lactate dehydrogenase(LDH),reactive oxygen species(ROS),protein carbonyl(PC),and malondialdehyde(MDA).Further investigations indicated that VA alleviated mitochondrial stress,potentially through suppressing the canonical UPR^(mt) axis while activating both the UPR^(mt) sirtuin axis and the UPRIMS/Erα axis.VA also modulated mitochondrial mass via multiple mechanisms,including the promotion of mitochondrial biogenesis,maintenance of dynamics by stimulating fusion and reducing fission,and inhibition of mitophagy.The suppression of mitophagy likely involved downregulating both the Pink1/Parkin-dependent pathway and the Hif1a-Bnip3 pathway.Taken together,these adaptations suggested an essential role for VA in preserving mitochondrial homeostasis.Based on the quadratic regression analysis of ROS and MDA levels from the hypoxic group,the estimated VA requirements for adult grass carp were 2,013 and 2,056 IU/kg diet,respectively.Conclusions In summary,this study provided the first evidence that VA conferred protective effects against hypoxiainduced gill damage in grass carp.展开更多
In-situ stress is a key parameter for underground mine design and rock stability analysis.The borehole overcoring technique is widely used for in-situ stress measurement,but the rheological recovery deformation of roc...In-situ stress is a key parameter for underground mine design and rock stability analysis.The borehole overcoring technique is widely used for in-situ stress measurement,but the rheological recovery deformation of rocks after stress relief introduces errors.To improve accuracy,this study proposes an in-situ stress solution theory that incorporates time-dependent stress relief effects.Triaxial stepwise loadingunloading rheological tests on granite and siltstone established quantitative relationships between instantaneous elastic recovery and viscoelastic recovery under different stress levels,confirming their impact on measurement accuracy.By integrating a dual-class elastic deformation recovery model,an improved in-situ stress solution theory was derived.Additionally,accounting for the nonlinear characteristics of rock masses,a determination method for time-dependent nonlinear mechanical parameters was proposed.Based on the CSIRO hollow inclusion strain cell,time-dependent strain correction equations and long-term confining pressure calibration equations were formulated.Finally,the proposed theory was successfully applied at one iron mine(736 m depth)in Xinjiang,China,and one coal mine(510 m depth)in Ningxia,China.Compared to classical theory,the calculated mean stress values showed accuracy improvements of 6.0%and 9.4%,respectively,validating the applicability and reliability of the proposed theory.展开更多
Global population pressures have necessitated increased focus on protecting and developing resilient plant species that can maintain productivity despite environmental challenges.Environmental degradation,driven by cl...Global population pressures have necessitated increased focus on protecting and developing resilient plant species that can maintain productivity despite environmental challenges.Environmental degradation,driven by climate change and anthropogenic activities,poses significant threats to global food security through various forms of physical stress.Major environmental constraints affecting agricultural yields worldwide include salinity,water scarcity,nutritional imbalances(encompassing mineral toxicity and deficiencies),and extreme temperatures.Crop yield is influenced by multiple abiotic factors,including agronomic conditions,climatic variables,and soil nutrient availability.Plants develop various survival mechanisms at molecular,cellular,and physiological levels in response to stress.Abiotic stress,whether occurring individually or in combination,significantly impacts crop growth and productivity.For instance,drought stress reduces leaf area,plant height,and overall crop development.Cold stress inhibits plant development and crop efficiency,leading to diminished productivity.Salinity stress not only induces water stress in plants but also negatively affects cytosolic metabolism,cell development,membrane function,and increases reactive oxygen species(ROS)production.Elevated CO_(2)concentrations may enhance global precipitation patterns,potentially resulting in increased rainfall that can adversely affect crop development.Plants under excessive water stress exhibit reduced amylose content but increased crude protein levels.This affects both quality and quantity of crop production by inhibiting seed germination and causing growth impairment through combined effects of elevated osmotic potential and ion toxicity.Plants have evolved various escape-avoidance and tolerance mechanisms in response to abiotic stress,including physiological adaptations and integrated cellular or molecular responses.This review paper examines the impact of abiotic stress on morpho-physiological,biochemical,and molecular activities across various crops.Additionally,it analyzes crop interactions with abiotic stress regarding response and adaptation mechanisms,providing a fundamental framework for species selection and development of stress-tolerant varieties in the future.展开更多
Background:Childhood leukemia,a malignant proliferative disorder of the hematopoietic system and the most common childhood cancer,poses a significant threat to the lives and health of affected children.For parents,a l...Background:Childhood leukemia,a malignant proliferative disorder of the hematopoietic system and the most common childhood cancer,poses a significant threat to the lives and health of affected children.For parents,a leukemia diagnosis in their child is a profoundly traumatic event.As primary caregivers,they endure immense psychological distress and caregiving stress throughout the prolonged and demanding treatment process,which can adversely affect their own well-being and caregiving capacity.However,the psychological mechanisms,such as the role of mindfulness,linking caregiver stress to parental coping strategies remain underexplored,and evidence-based interventions to support these parents are needed.Methods:In Study 1,we administered a cross-sectional survey to 242 parents of children with leukemia who were hospitalized at the Affiliated Hospital of Qingdao University between January and August 2024.Participants completed measures assessing caregiver burden,mindful attention awareness,and parental coping style.In Study 2,we further evaluated the effects of a Mindfulness-Based Stress Reduction(MBSR)intervention.Results:The results of Study 1 revealed:(1)The caregiving stress significantly and negatively predicted coping style(β=−1.18,95%CI[−2.18,−0.18],p<0.01).(2)Caregiving stress also significantly and negatively predicted mindfulness(β=−1.90,95%CI[−2.43,−1.38],p<0.01).(3)Conversely,mindfulness significantly and positively predicted coping style(β=0.85,95%CI[0.62,1.07],p<0.01).These findings suggest that mindfulness mediates the relationship between caregiver burden and coping style.In Study 2,the experimental group showed a significant decrease in caregiver stress post-intervention(t=2.24,p<0.05),a significant increase in mindfulness(t=−4.61,p<0.001),and a significant improvement in coping style(t=−2.36,p<0.01).No significant changes were observed in the control group.Conclusion:MBSR can effectively enhance mindfulness and promote adaptive coping strategies,while reducing caregiver burden among parents of children with leukemia.展开更多
We investigated the influence of historical earthquakes on the 2022 Luding M_(S)6.8 earthquake and its subsequent effects.We computed the viscoelastic Coulomb stress changes induced by these historical seismic events ...We investigated the influence of historical earthquakes on the 2022 Luding M_(S)6.8 earthquake and its subsequent effects.We computed the viscoelastic Coulomb stress changes induced by these historical seismic events using the rupture model of historical earthquakes and the layered Maxwell viscoelastic medium model.Our findings indicate that the Luding earthquake was brought forward approximately 29 years because of several historical earthquakes.Specifically,the 1923 Renda M_(S)7.3 earthquake,the 1933 Diexi M_(S)7.5 earthquake,the 1973 Luhuo M_(S)7.3 earthquake,the 2008 Kangding M_(S)5.1 earthquake,the 2008 Wenchuan M_(S)8.0 earthquake,the 2014 Kangding M_(S)6.3 earthquake,and the 2014 Kangding M_(S)5.8 earthquake advanced the occurrence of the event by 117.61,26.67,84.51,0.27,0.91,7.64,and 3.17 years,respectively.Conversely,the 1936 Mabian earthquake swarm,the 1948 Litang M_(S)7.3 earthquake,the 1955 Kangding M_(S)7.5 earthquake,and the 2013 Lushan M_(S)7.0 earthquake delayed its occurrence by 39.89,22.43,144.23,and 4.89 years,respectively.Furthermore,by employing the halfspace homogeneous elastic model and the rupture characteristics of the Luding earthquake,we computed the coseismic Coulomb stress changes in neighboring faults.Our results reveal increased Coulomb stress on the Xianshuihe fault(excluding its southern segment),the Anninghe fault,the Zemuhe fault,the Daliangshan fault,the southern segment of the Longmenshan fault,the northern segment of the Mabian-Yanjin fault,and the Xiaojinhe fault.Conversely,we observed stress decreases in the southern segment of the Jinshajiang fault,the central and eastern segments of the Longriba fault,the Mabian-Yanjin fault(excluding its northern segment),and the southern segment of the Xianshuihe fault.展开更多
Crop yield and quality are affected by abiotic stresses such as drought,low and high temperature,salinity,and heavy metals,which threaten the survival of human beings and the development of industry.As a new plant hor...Crop yield and quality are affected by abiotic stresses such as drought,low and high temperature,salinity,and heavy metals,which threaten the survival of human beings and the development of industry.As a new plant hormone derived from carotenoid,strigolactone(SL)is produced in the roots of plants.It was first reported that SL can induce seed germination of root-parasitic plants.In recent years,it has been shown that strigolactone plays a regulatory role in plant response to abiotic stresses.By eliminating oxidative stress caused by reactive oxygen species,it can potentially increase photosynthetic rate,chlorophyll content,and thus enhance plant drought resistance.Transcriptome studies have explored signal transduction,antioxidant enzyme activity,transcription factors,and expression of stress-and metabolism-related genes induced by extrinsic strigolactone in plants,the effects of strigolactone on plant growth and development have been preliminarily determined,but the studies on inducing crop tolerance to abiotic stresses are still unknown.In this review,the physiological and molecular aspects of the induction of the response to stress in horticultural crops by strigolactone were reviewed.It is important to improve the tolerance and productivity of horticultural crops under abiotic stress.展开更多
Water inrush hazards from the floor strata of longwall workingface are commonly encountered in North China coalfields,which essentially result from the evolution of permeability in the floor rock under complex mining-...Water inrush hazards from the floor strata of longwall workingface are commonly encountered in North China coalfields,which essentially result from the evolution of permeability in the floor rock under complex mining-induced stress conditions.Current research rarely addresses the evolution of rock permeability under such complex stress paths.Describing this evolution using only one stress parameter,such as effective stress,deviatoric stress,axial stress,or confining stress,is highly challenging.In this study,we developed a laboratory loading scheme that simulates mining-induced stress evolution.Hydro-mechanical experiments were conducted to investigate the evolution of rock permeability under mining stress.The mechanism on the change of stress-permeability relationships in mining-disturbed rock is revealed,supporting to the analysis of management strategies for floor water-inrush disasters.The results show that rock permeability evolves through four stages,including rapid decline,gradual fluctuation,sharp increase,and slow attenuation.1–2 permeability surges occurred during mining-stress loading,closely linked to the emergence and reversal of deviatoric stress in magnitude and direction.With the first permeability surge,the deviatoric stress within the mudstone reached approximately 1.7 MPa,whereas that of the sandstone was about 1 MPa.The second permeability surge in the mudstone corresponded to the secondary rotation of the principal stress direction.CT and ultrasonic tests suggested an increase in microcracks in both rocks during the first permeability surge.However,the deviatoric stress-permeability plot before and after mining indicated that the fracture of mudstone sample changed significantly,while that of the sandstone remained unchanged.The permeability surges observed at different stages are interpreted as resulting from shear-induced reopening of pre-existing fractures and the formation of new shear-failure fractures.A stress-permeability model jointly governed by effective mean stress and deviatoric stress was established.Furthermore,two strategies are proposed for the floor water-inrush disasters prevention,(i)timely backfilling to reduce deviatoric stress,(ii)grouting after the first permeability surge.This work provides insights into stress-seepage behavior in rocks under complex stress evolution and offers new perspectives for identifying potential water inrush pathways in the floor strata of coal seam during longwall mining.展开更多
Prohibitin(PHB)plays critical roles in plant growth and development.In this study,we utilized CRISPR/Cas9 gene-editing technology to generate homozygous OsPHB2 knockout transgenic plants,designated cr-osphb2.The cr-os...Prohibitin(PHB)plays critical roles in plant growth and development.In this study,we utilized CRISPR/Cas9 gene-editing technology to generate homozygous OsPHB2 knockout transgenic plants,designated cr-osphb2.The cr-osphb2 line exhibited wider leaves,dwarfism,and shorter panicles.Subcellular localization results indicated that OsPHB2 localizes to mitochondria.Under salt stress conditions,cr-osphb2 exhibited enhanced tolerance.Haplotype(Hap)analysis identified three major Haps(Hap1,Hap2,and Hap3)of OsPHB2,among which Hap2 was associated with a greater number of effective panicles and higher yield,indicating its potential value for breeding applications.Collectively,our findings demonstrate that OsPHB2 plays an important role in regulating growth,development,and salt stress responses in rice.展开更多
As a specific spoilage organism of seafood under refrigerated temperature conditions,Shewanella spp.tend to form biofilms that exacerbate the occurrence of seafood spoilage.Biofilm-promoting factor A(BpfA)has been rep...As a specific spoilage organism of seafood under refrigerated temperature conditions,Shewanella spp.tend to form biofilms that exacerbate the occurrence of seafood spoilage.Biofilm-promoting factor A(BpfA)has been reported to promote the adhesion and biofilm formation of Shewanella spp.,but its role in adhesion and biofilm formation of S.putrefaciens under cold stress needs to be further investigated.To better comprehend the effect of BpfA on adhesion and biofilm formation of S.putrefaciens under cold stress(4℃),bacterial adhesion and biofilm phenotype of S.putrefaciens CN32 WT andΔbpfA at 4℃were analyzed and performed transcriptomics.The results showed that the deletion of bpfA had almost no effect on the growth of S.putrefaciens CN32 at 4℃,but weakened the unicellular adhesion capacity of S.putrefaciens CN32 and destabilized the stability of the multicellular adhesion layer.In addition,the biomass of the mature biofilm formed byΔbpfA was merely around 50%of that observed in the mature biofilm of S.putrefaciens CN32 WT,the average thickness and volume of the biofilm decreased by 18%and 27%,respectively,and the composition of the biofilm changed.Transcriptome analysis demonstrated that the deletion of bpfA led to differential expression of genes involved in metabolic pathways such as bacterial chemotaxis,two-component system,tyrosine metabolism,drug metabolism-other enzymes and biofilm formation-Vibrio cholerae,which in turn influenced bacterial adhesion and biofilm formation.Those results advance our acknowledgment of the character of BpfA on adhesion and biofilm formation of S.putrefaciens CN32,which contributes to understanding bacterial adhesion and the control of biofilm formation.展开更多
Background Heat stress(HS)can impair boar testicular function,leading to reproductive issues.However,chlorogenic acid(CGA)has been shown to mitigate HS-induced damage in various livestock and poultry species.Prepubert...Background Heat stress(HS)can impair boar testicular function,leading to reproductive issues.However,chlorogenic acid(CGA)has been shown to mitigate HS-induced damage in various livestock and poultry species.Prepuberty is an important stage of testicular development in boars after birth.However,the protective effect of CGA on testicular HS injury during prepuberty boars and the underlying mechanisms are still not fully understood.Results In vivo,a total of 30 healthy boars with similar body weights and ages were obtained and randomly divided into 3 groups,which were fed a basal diet supplemented with CGA 0(the ND_TN group),0(the ND_HS group)or 1,000(the CGA_HS group)mg/kg.After being fed for 28 d,all the groups,except the ND_TN group,were treated with high temperature for 7 d,after which samples were collected from the boars and analysed.The results showed that CGA significantly mitigated the HS-induced reduction in T-AOC content in testicular tissue and sperm density.Mechanistically,multiomics analysis revealed that the genes differentially expressed by CGA and HS were predominantly associated with the glutathione metabolism pathway.The combined analysis of transcriptomics and proteomics revealed that only BLVRA was affected by both HS and CGA when the mRNA and protein levels of a gene showed differential expression with the same trend.In vitro studies confirmed that CGA modulated GPX3 expression via BLVRA,affected GPx activity,and attenuated HS-induced ROS accumulation.Conclusions In conclusion,prepubertal HS impairs the spermatogenic capacity of boars.BLVRA may mediate the testicular protective effect of CGA,although in vivo validation of this pathway is needed.This study contributes to elucidating the mechanisms underlying the effects of HS on prepubertal boar testicular development using multiomics approaches,laying a foundation for the potential utilization of CGA in swine production.展开更多
Ceratitis capitata(Wiedemann)is a cosmopolitan pest of economic importance.It is controlled by using the Sterile Insect Technique(SIT),which involves rearing and release of sterile males destined to mate with wild fem...Ceratitis capitata(Wiedemann)is a cosmopolitan pest of economic importance.It is controlled by using the Sterile Insect Technique(SIT),which involves rearing and release of sterile males destined to mate with wild females,causing generation-to-generation suppression.Medflies are colonized by microorganisms,primarily the Enterobacteriaceae,with the genera Klebsiella and Enterobacter being the most common.Such microbiota contributes to host fitness.During the SIT,diet with antibiotics and irradiation for sterility of adults alter microbiota.We aimed to determine the role of Medfly microbiota on resistance to abiotic stress conditions,evaluating its function under:(i)starvation,(ii)elevated temperatures,and(iii)dry environments.These conditions simulate challenges Medfly may encounter after release,which differ from controlled rearing environments.We compared adult survival between symbiotic and aposymbiotic individuals,under starvation,two thermal regimes(25 and 30℃)or two humidity regimes(20%-25%and 80%-90%R.H.).Aposymbiotic individuals were obtained after providing them with water containing a mixture of antibiotics and methylparaben.Treatment with antimicrobials effectively reduced the gut microbiota.While starvation had no significant effect on survival,a higher proportion of aposymbiotic individuals died earlier at 30℃ and under dry humidity,with the effect being more pronounced after 48 h.Our results suggest that microbiota plays a role in adaptation of Medfly under environmental stress.We report for the presence of a culturable yeast in the digestive tract of C.capitata,Zygosaccharomyces rouxii.Providing a probiotic adult diet with bacteria and Z.rouxii prior to release could improve SIT outcomes under adverse conditions.展开更多
To address the key scientific challenge of monitoring the dynamic fracturing of surrounding rock in deep roadways,this study systematically investigates the quantitative relationship between stress and charge signals ...To address the key scientific challenge of monitoring the dynamic fracturing of surrounding rock in deep roadways,this study systematically investigates the quantitative relationship between stress and charge signals during coal mass loading.By integrating innovative analytical approaches,introducing quantitative evaluation indices,and developing a charge–stress inversion model,and incorporating underground monitoring practices,significant progress has been achieved in elucidating the correlation between stress variations and charge signals throughout the entire coal mass fracturing process.First,in the field of stress–charge correlation analysis,empirical mode decomposition(EMD)was combined with wavelet coherence analysis for the first time,enabling the removal of slow-varying stress trends while retaining high-frequency fluctuations.This approach allowed for the quantitative characterization of the evolution of coherence between stress variations and charge fluctuations across multiple time scales.Second,coherence skewness and the proportion of high-coherence intervals were innovatively introduced to examine the influence of time scale selection on correlation results.On this basis,a criterion for determining the near-optimal observation scale of charge signals was proposed,providing a quantitative reference for time scale selection in similar signal analyses.Finally,by correlating charge signals with coal damage factors and stress states,a charge-based damage evolution equation was established to achieve effective stress inversion.Combined with in situ monitoring of stress and charge in roadway surrounding rock,this approach revealed the correlation characteristics of stress and charge intensity responses during the dynamic fracturing process.The results indicate,first,that charge signals are not significantly correlated with the absolute stress level of coal but are directly associated with stress variations following coal damage and failure,with the amplitude of charge fluctuations increasing alongside stress fluctuations.Second,coherence between stress and charge signals varies markedly across time scales,with excessively small or large scales leading to distortion,and the scale corresponding to the peak proportion of intervals with coherence>0.8 was identified as the near-optimal observation scale.Third,charge signals can effectively characterize coal damage factors,and the established damage evolution equation can effectively invert stress variation trends.Fourth,in underground roadways,zones of dynamic fracturing in surrounding rock are commonly located in areas where stress concentration overlaps with regions of high charge intensity,further confirming the strong consistency between charge and stress variations.These findings improve the theoretical framework of charge signal responses in loaded coal and provide a scientific basis for precise“stress-charge”monitoring of dynamic disasters,offering practical potential for engineering applications.展开更多
基金Projects(52222810,52178383)supported by the National Natural Science Foundation of China。
文摘Axial chain rockbursts(ACRs)repeatedly occur in deep tunnels during drilling and blasting methodology(D&B)within locked-in stress zones,severely hindering construction progress.In extremely cases,ACRs can persist for 7−10 d and affect areas exceeding 20 m along tunnel axis.Through integrated geological investigations and microseismic(MS)monitoring,the geological characteristics,MS activity patterns,and formation mechanisms of ACRs were analyzed.In tectonically active regions,locked-in stress zones arise from interactions between multiple structural planes.Blasting dynamic disturbances during tunnel excavation in these zones trigger early slippage along structural planes and fractures in the surrounding rock,with MS events developing ahead of the working face.High-energy MS events dominate during the development and occurrence stages of ACRs,extending 20−30 m(3−4 tunnel diameters)ahead of the working face.Following the ACRs,low-energy MS events primarily occur behind the working face.Tensile fracturing is the predominant failure mode during ACRs.Shear and mixed fractures primarily occur within the ACRs zone during the intra-ACR phase.Monitoring MS event locations ahead of the working face provides a reliable approach for prewarning potential ACR-prone zones.
文摘Rocks are composed of mineral particles and micropores between mineral which has a great influence on the mechanical properties of rocks. In this paper, based on the theory of locked-in stress developed by academician Chen Zongji, the locked-in stress problem in underground rock is simulated by the thermal expansion of hard rubber particles. The pore inclusion in rock is assumed to be uniformly distributed spherical cavities. Using the thermal stress theory, the stress of rock with a spherical pore inclusion is equivalent to the thermal stress generated by the spherical hard rubber inclusion. The elastic theory formula of the temperature increment and the equivalent pore pressure of the spherical hard rubber inclusion is derived. The numerical simulation of the rock mass model with a spherical hard rubber inclusion is carried out and compared to the theoretical calculation results<span lang="EN-US" style="font-family:;" minion="" pro="" capt",serif;font-size:10pt;mso-fareast-font-family:宋体;mso-bidi-font-family:"times="" new="" roman";mso-ansi-language:en-us;mso-fareast-language:zh-cn;mso-bidi-language:ar-sa;mso-bidi-font-weight:bold;"="">;</span><span lang="EN-US" style="font-family:;" minion="" pro="" capt",serif;font-size:10pt;mso-fareast-font-family:宋体;mso-bidi-font-family:"times="" new="" roman";mso-ansi-language:en-us;mso-fareast-language:en-us;mso-bidi-language:ar-sa;mso-bidi-font-weight:bold;"=""> the results show that they are consistent. The method proposed by this paper for simulating stress distribution in rock by thermal stress is reasonable and feasible</span><span lang="EN-US" style="font-family:;" minion="" pro="" capt",serif;font-size:10pt;mso-fareast-font-family:宋体;mso-bidi-font-family:"times="" new="" roman";mso-ansi-language:en-us;mso-fareast-language:zh-cn;mso-bidi-language:ar-sa;mso-bidi-font-weight:bold;"="">;</span><span lang="EN-US" style="font-family:;" minion="" pro="" capt",serif;font-size:10pt;mso-fareast-font-family:宋体;mso-bidi-font-family:"times="" new="" roman";mso-ansi-language:en-us;mso-fareast-language:en-us;mso-bidi-language:ar-sa;mso-bidi-font-weight:bold;"=""> it has a positive meaning for further study of mechanic phenomenon of rock with micropore inclusion.</span>
基金supported by the Project of Sanya Yazhou Bay Science and Technology City (SKJC-JYRC-2024-26)the National Natural Science Foundation of China (32460072)+4 种基金Hainan Provincial Natural Science Foundation of China (323RC421)the Hainan Province Science and Technology Special Fund (ZDYF2022XDNY144)the Hainan Provincial Academician Innovation Platform Project (HDYSZX-202004)the Collaborative Innovation Center of Nanfan and High-Efficiency Tropical Agriculture, Hainan University (XTCX2022NYB06)Hainan Postdoctoral Research Grant Project
文摘In light of the pressing global challenges of climate change,declining crop resilience,and hidden hunger,it is imperative to overcome the limitations of conventional crop breeding to enhance both the nutritional quality and stress tolerance of crops.Synthetic metabolic engineering presents innovative strategies for the precision modification and de novo design of metabolic pathways.This approach generally encompasses three essential steps:identifying key metabolites through metabolomics,integrating multi-omics technologies to investigate the synthesis and regulation of these metabolites,and utilizing gene editing or de novo design to modify crop metabolic pathways associated with desirable agronomic traits.This review underscores the vital role of plant metabolite diversity in enhancing crop nutritional quality and stress resilience.Integrated multi-omics analyses facilitate the metabolic engineering by identifying key genes,transporters,and transcription factors that regulate metabolite biosynthesis.Precision modification strategies employ genome editing tools to reprogram endogenous metabolic networks,while de novo design reconstructs metabolic pathways through the introduction of exogenous biological elements—thereby both approaches enable the targeted enhancement of desired traits.These strategies have been effectively implemented in major food crops.However,simultaneously enhancing nutritional quality and stress resilience remains challenging due to inherent trade-offs and resource competition in distinct metabolic pathways within plants.Future research should integrate AI-driven predictive models with multi-omics datasets to decipher dynamic metabolic homeostasis and engineer climate-smart crops that maximize yield while preserving quality and environmental adaptability.
基金financially supported by the Sichuan Science and Technology Program(2024YFNH0016,2024NSFSC2072)the earmarked fund for CARS(CARS-45)+2 种基金the National Science Fund for Distinguished Young Scholars of China(32425056)the National Key Research and Development Program of China(2023YFD2400600)Sichuan Innovation Team of National Modern Agricultural Industry Technology System(SCCXTD-2024-15)。
文摘Background Environmental hypoxia is a common phenomenon in aquaculture,which causes gill tissue injury in fish.Glutathione(GSH)is a vital antioxidant in animal tissues,and its levels decrease under hypoxic conditions.However,the effects of glutathione on fish under hypoxic stress remain poorly understood.This study aimed to investigate the impact of glutathione on gill tissue damage in fish under hypoxic stress and explore the underlying mechanisms.Methods Six experimental diets with varying glutathione concentrations.The actual glutathione levels in these diets,measured by high-performance liquid chromatography,were 0.00,145.95,291.90,437.85,583.80,and 729.75 mg/kg,respectively.Fish were fed these diets for 70 d,after which a 96-h hypoxic stress experiment was conducted.The experiment was set up with normoxic and hypoxic groups,in which the dissolved oxygen in the group was 6 mg/L,and that in the group was 1 mg/L.Results This research revealed that glutathione could enhance the growth performance and antioxidant capability of juvenile grass carp while mitigating the structural damage to gill tissues induced by hypoxia stress.Mechanistic investigations further indicated that glutathione mitigated hypoxia-induced oxidative injury in gill tissues and improved their antioxidant capacity.In addition,glutathione attenuated gill apoptosis induced by hypoxia stress.Glutathione also inhibited the initiation,nucleation,elongation,and degradation phases of autophagy,thereby attenuating hypoxia-induced gill autophagy.Moreover,glutathione was found to alleviate hypoxia-induced endoplasmic reticulum stress(ERS)in gills,a response potentially linked to the suppression of PERK,IRE1,and ATF6 signaling pathways.Finally,based on the ROS and PC contents in gill tissue,the optimum glutathione supplementation levels for juvenile grass carp under hypoxia stress were 437.10 and 495.00 mg/kg,respectively.Conclusions In conclusion,our experimental results demonstrated the effectiveness of glutathione in alleviating gill tissue damage caused by hypoxic stress.This study confirms the feasibility and effectiveness of dietary glutathione addition to alleviate hypoxic stress in fish.
基金supported by the National Natural Science Foundation of China(Grant Nos.32160501 and 32201901)the Accelerated Breeding Initiative of the Consultative Group on International Agricultural Research(Grant No.INIT-01)+2 种基金the Natural Science Foundation of Guangxi,China(Grant No.2021GXNSFAA220026)the Program on National Modern Agricultural Technology System Guangxi Innovation Team,China(Grant No.nycytxgxcxtd-2021-01-04)the Advantage Team Project of Guangxi Academy of Agricultural Sciences,China(Grant No.2026YT070).
文摘Rice production is increasingly challenged by flooding stress because of global warming and rising sea levels.As the world’s most important staple crop,rice is highly vulnerable to anaerobic and submergence conditions that occur during flooding,particularly at the germination and vegetative stages.Anaerobic environments hinder seedling establishment during germination,while prolonged submergence during the vegetative stage impairs growth,ultimately reducing yield and grain quality.These stresses,driven by extended inundation,trigger a cascade of detrimental physiological responses and represent a major barrier to stable rice production and global food security.In this review,we examine the effects of flooding on rice growth at both the germination and vegetative stages.We further summarize recent advances in the identification of flooding-tolerant germplasm,QTL mapping,genome-wide association study,transcriptomic and proteomic analyses,and other molecular studies.Subsequently,we highlight potential cultivation and regulatory strategies,including genetic,morphological,physiological,and endogenous hormone-related approaches,aimed at enhancing tolerance to anaerobic and submergence stress.Together,these approaches underscore the promise of integrating molecular insights with agronomic practices to mitigate flooding damage and support sustainable rice production.
基金supported by the National Natural Science Foundation of China(Grant No.52274148)the Natural Science Foundation of Hebei Province(Grant No.E2023402103)+1 种基金the Science Research Project of Hebei Education Department(Grant No.QN2025247)Handan Science and Technology R&D Project(Grant No.23422093047)。
文摘Asymmetric deformation and failure of surrounding rock are frequently observed in mountain tunnels and deep mining roadways,yet the underlying mechanisms remain poorly understood.To investigate asymmetric failure in roadways adjacent to fault structures and mining panels,this study adopts an integrated approach combining theoretical derivation,numerical simulation,and field application,with particular emphasis on the second invariant of the stress deviator(J_(2) )in the surrounding rock.Based on the stress solution for a circular opening,an analytical expression for J_(2)(distortion energy)is derived by considering the reorientation of principal stresses.The study demonstrates that both the increase and reorientation of principal stresses induced by fault–mining interaction jointly govern the spatial distribution of J_(2) and the resulting asymmetric failure behavior.Specifically,the principal stress rotation angle determines the location of J_(2) concentration,whereas the principal stress ratio controls its magnitude.To mitigate asymmetric failure,it is recommended to optimize the J_(2) state through adjustments in roadway size,geometry,and support systems,while simultaneously controlling the asymmetric concentration of stress deviator to enhance roadway stability.This study systematically elucidates the chain mechanism of asymmetric surrounding rock failure driven by principal stress,and further proposes a rational asymmetric joint control strategy,providing theoretical guidance for similar underground engineering conditions.
文摘Protein aggregates,mitochondrial import stress and neurodegenerative disorders:A salient hallmark of several neurodegenerative diseases,including Parkinson’s disease,is the abundance of protein aggregates(Goiran et al.,2022).This molecular event is believed to lead to activation of stress pathways ultimately resulting in cellular dysfunction(Eldeeb et al.,2022).Accordingly,many lines of research investigations focused on dampening the formation of protein aggregates or augmenting the clearance of protein aggregates as a potential therapeutic strategy to counteract the progression of neurodegenerative diseases,albeit with little success(Costa-Mattioli and Walter,2020).Cell stress cues such as the accumulation of protein aggregates lead to the activation of stress response pathways that aid cells in responding to the damage.Despite the notion that the transient activation of these pathways helps cells cope with stressors,persistent activation can induce unwanted apoptosis of cells and reduce overall tissue strength as well as lead to an accumulation of aggregation-prone proteins(Hetz and Papa,2018).Mutations in proteins involved in stress signaling termination can cause conditions like ataxia and early-onset dementia(Conroy et al.,2014).Therefore,it is crucial for stress response signaling to be turned off once conditions have improved.Nevertheless,the mechanisms by which cells silence these signals are still elusive.
基金support from the Postgraduate Research&Practice Innovation Program of Jiangsu Province,China(Grant No.KYCX24_2822)the Graduate Innovation Program of China University of Mining and Technology(Grant No.2024WLKXJ205)the National Natural Science Foundation of China(Grant No.52474157).
文摘Excavation causes stress redistribution and affects the stress path during the shearing process of rock.The shear strength of rock varies under different stress paths,and the presence of defects reduces the shear strength.To further investigate this phenomenon,this study investigates the shear behaviour of rocks with different shear surface integrities under the influenceof different stress paths through laboratory tests and numerical simulations.The results indicate that the shear strength depends on the stress path and a decrease in the shear surface integrity reduces the degree of dependence.The cohesion and friction angle of the Mohr‒Coulomb criterion decrease with weakening of the shear surface integrity.For different stress paths,the direct shear strength is always greater than that of other shear stress paths.The pattern of changes in the acoustic emission count and cumulative count indirectly reflectsthe above findings.Numerical simulations further indicate that the different principal stress states and normal suppression effects during the shearing process lead to changes in the factors of crack propagation,resulting in different mechanical behaviours under various stress paths.For rocks with different integrity levels,the main reason for the different path dependences of shear strength is that the size of the area affected by shear is different.Shear failure will concentrate on the shear plane when the normal inhibition effect is greater.This study explores the mechanism of rock shear behaviour,providing a theoretical basis for establishing more accurate constitutive models and strength criteria.
基金funded by the Sichuan Science and Technology Program(2024YFNH0016)the earmarked fund for CARS(CARS-45)+1 种基金the National Science Fund for Distinguished Young Scholars of China(32425056)the National Key R&D Program of China(2023YFD2400600)。
文摘Background Hypoxia is a pervasive challenge in aquaculture that poses a significant threat to aquatic organisms.Since fish cannot synthesize vitamin A endogenously,it must be supplied through diet,and it plays a vital role in supporting fish stress resistance.This study aimed to investigate the protective effects of VA on the gills of adult grass carp(Ctenopharyngodon idella)against hypoxia and to elucidate the underlying mechanisms.Methods Six experimental diets with graded VA levels(375,862,1,614,2,099,2,786,and 3,118 IU/kg)were fed to grass carp(initial weight:726±1.2 g)for 60 d.After the trial,24 fish per treatment were selected,divided equally into normoxic and hypoxic groups,fasted for 24 h,and then subjected to a 96-h acute hypoxic challenge.Results The results demonstrated that VA supplementation mitigated hypoxia-induced damage in gill tissue,as evidenced by histological examination.Furthermore,VA alleviated oxidative stress,as indicated by reduced levels of lactate(LD),lactate dehydrogenase(LDH),reactive oxygen species(ROS),protein carbonyl(PC),and malondialdehyde(MDA).Further investigations indicated that VA alleviated mitochondrial stress,potentially through suppressing the canonical UPR^(mt) axis while activating both the UPR^(mt) sirtuin axis and the UPRIMS/Erα axis.VA also modulated mitochondrial mass via multiple mechanisms,including the promotion of mitochondrial biogenesis,maintenance of dynamics by stimulating fusion and reducing fission,and inhibition of mitophagy.The suppression of mitophagy likely involved downregulating both the Pink1/Parkin-dependent pathway and the Hif1a-Bnip3 pathway.Taken together,these adaptations suggested an essential role for VA in preserving mitochondrial homeostasis.Based on the quadratic regression analysis of ROS and MDA levels from the hypoxic group,the estimated VA requirements for adult grass carp were 2,013 and 2,056 IU/kg diet,respectively.Conclusions In summary,this study provided the first evidence that VA conferred protective effects against hypoxiainduced gill damage in grass carp.
基金supported by the National Science and Technology Major Project of the Ministry of Science and Technology of China(No.2024ZD1700201)the National Natural Science Foundation of China(Nos.U2034206,51974014 and 51574014)+1 种基金the Guangdong Basic and Applied Basic Research Foundation(No.2024A1515011631)the National Key Research and Development Project of China(No.2022YFC3004601)。
文摘In-situ stress is a key parameter for underground mine design and rock stability analysis.The borehole overcoring technique is widely used for in-situ stress measurement,but the rheological recovery deformation of rocks after stress relief introduces errors.To improve accuracy,this study proposes an in-situ stress solution theory that incorporates time-dependent stress relief effects.Triaxial stepwise loadingunloading rheological tests on granite and siltstone established quantitative relationships between instantaneous elastic recovery and viscoelastic recovery under different stress levels,confirming their impact on measurement accuracy.By integrating a dual-class elastic deformation recovery model,an improved in-situ stress solution theory was derived.Additionally,accounting for the nonlinear characteristics of rock masses,a determination method for time-dependent nonlinear mechanical parameters was proposed.Based on the CSIRO hollow inclusion strain cell,time-dependent strain correction equations and long-term confining pressure calibration equations were formulated.Finally,the proposed theory was successfully applied at one iron mine(736 m depth)in Xinjiang,China,and one coal mine(510 m depth)in Ningxia,China.Compared to classical theory,the calculated mean stress values showed accuracy improvements of 6.0%and 9.4%,respectively,validating the applicability and reliability of the proposed theory.
基金financially supported by the National Key R&D Program of China(2022YFE0113400)the Jiangsu Provincial Fund for Realizing Carbon Emission Peaking and Neutralization,China(BE2022305)+1 种基金the National Natural Science Fundation of China(32102411)the Project funded by China Postdoctoral Science Foundation(2022M722698)。
文摘Global population pressures have necessitated increased focus on protecting and developing resilient plant species that can maintain productivity despite environmental challenges.Environmental degradation,driven by climate change and anthropogenic activities,poses significant threats to global food security through various forms of physical stress.Major environmental constraints affecting agricultural yields worldwide include salinity,water scarcity,nutritional imbalances(encompassing mineral toxicity and deficiencies),and extreme temperatures.Crop yield is influenced by multiple abiotic factors,including agronomic conditions,climatic variables,and soil nutrient availability.Plants develop various survival mechanisms at molecular,cellular,and physiological levels in response to stress.Abiotic stress,whether occurring individually or in combination,significantly impacts crop growth and productivity.For instance,drought stress reduces leaf area,plant height,and overall crop development.Cold stress inhibits plant development and crop efficiency,leading to diminished productivity.Salinity stress not only induces water stress in plants but also negatively affects cytosolic metabolism,cell development,membrane function,and increases reactive oxygen species(ROS)production.Elevated CO_(2)concentrations may enhance global precipitation patterns,potentially resulting in increased rainfall that can adversely affect crop development.Plants under excessive water stress exhibit reduced amylose content but increased crude protein levels.This affects both quality and quantity of crop production by inhibiting seed germination and causing growth impairment through combined effects of elevated osmotic potential and ion toxicity.Plants have evolved various escape-avoidance and tolerance mechanisms in response to abiotic stress,including physiological adaptations and integrated cellular or molecular responses.This review paper examines the impact of abiotic stress on morpho-physiological,biochemical,and molecular activities across various crops.Additionally,it analyzes crop interactions with abiotic stress regarding response and adaptation mechanisms,providing a fundamental framework for species selection and development of stress-tolerant varieties in the future.
基金approved by the Ethics Review Committee of Northwest Normal University(Approval No.:[2023028]).All participants signed the informed consent in this study.
文摘Background:Childhood leukemia,a malignant proliferative disorder of the hematopoietic system and the most common childhood cancer,poses a significant threat to the lives and health of affected children.For parents,a leukemia diagnosis in their child is a profoundly traumatic event.As primary caregivers,they endure immense psychological distress and caregiving stress throughout the prolonged and demanding treatment process,which can adversely affect their own well-being and caregiving capacity.However,the psychological mechanisms,such as the role of mindfulness,linking caregiver stress to parental coping strategies remain underexplored,and evidence-based interventions to support these parents are needed.Methods:In Study 1,we administered a cross-sectional survey to 242 parents of children with leukemia who were hospitalized at the Affiliated Hospital of Qingdao University between January and August 2024.Participants completed measures assessing caregiver burden,mindful attention awareness,and parental coping style.In Study 2,we further evaluated the effects of a Mindfulness-Based Stress Reduction(MBSR)intervention.Results:The results of Study 1 revealed:(1)The caregiving stress significantly and negatively predicted coping style(β=−1.18,95%CI[−2.18,−0.18],p<0.01).(2)Caregiving stress also significantly and negatively predicted mindfulness(β=−1.90,95%CI[−2.43,−1.38],p<0.01).(3)Conversely,mindfulness significantly and positively predicted coping style(β=0.85,95%CI[0.62,1.07],p<0.01).These findings suggest that mindfulness mediates the relationship between caregiver burden and coping style.In Study 2,the experimental group showed a significant decrease in caregiver stress post-intervention(t=2.24,p<0.05),a significant increase in mindfulness(t=−4.61,p<0.001),and a significant improvement in coping style(t=−2.36,p<0.01).No significant changes were observed in the control group.Conclusion:MBSR can effectively enhance mindfulness and promote adaptive coping strategies,while reducing caregiver burden among parents of children with leukemia.
基金supported by the National Natural Science Foundation of China(Nos.42174074,and 41674055).
文摘We investigated the influence of historical earthquakes on the 2022 Luding M_(S)6.8 earthquake and its subsequent effects.We computed the viscoelastic Coulomb stress changes induced by these historical seismic events using the rupture model of historical earthquakes and the layered Maxwell viscoelastic medium model.Our findings indicate that the Luding earthquake was brought forward approximately 29 years because of several historical earthquakes.Specifically,the 1923 Renda M_(S)7.3 earthquake,the 1933 Diexi M_(S)7.5 earthquake,the 1973 Luhuo M_(S)7.3 earthquake,the 2008 Kangding M_(S)5.1 earthquake,the 2008 Wenchuan M_(S)8.0 earthquake,the 2014 Kangding M_(S)6.3 earthquake,and the 2014 Kangding M_(S)5.8 earthquake advanced the occurrence of the event by 117.61,26.67,84.51,0.27,0.91,7.64,and 3.17 years,respectively.Conversely,the 1936 Mabian earthquake swarm,the 1948 Litang M_(S)7.3 earthquake,the 1955 Kangding M_(S)7.5 earthquake,and the 2013 Lushan M_(S)7.0 earthquake delayed its occurrence by 39.89,22.43,144.23,and 4.89 years,respectively.Furthermore,by employing the halfspace homogeneous elastic model and the rupture characteristics of the Luding earthquake,we computed the coseismic Coulomb stress changes in neighboring faults.Our results reveal increased Coulomb stress on the Xianshuihe fault(excluding its southern segment),the Anninghe fault,the Zemuhe fault,the Daliangshan fault,the southern segment of the Longmenshan fault,the northern segment of the Mabian-Yanjin fault,and the Xiaojinhe fault.Conversely,we observed stress decreases in the southern segment of the Jinshajiang fault,the central and eastern segments of the Longriba fault,the Mabian-Yanjin fault(excluding its northern segment),and the southern segment of the Xianshuihe fault.
基金supported by the Special projects on biological seed industry and intensive processing of agricultural products(Grant No.202402AE090004-02)National Key R&D Program of China(Grant No.2023YFD2001404)+1 种基金Technology talent and platform program(Grant No.202305AF150112)China Agriculture Research System of MOF and MARA(Grant No.CARS-29-zp-6).
文摘Crop yield and quality are affected by abiotic stresses such as drought,low and high temperature,salinity,and heavy metals,which threaten the survival of human beings and the development of industry.As a new plant hormone derived from carotenoid,strigolactone(SL)is produced in the roots of plants.It was first reported that SL can induce seed germination of root-parasitic plants.In recent years,it has been shown that strigolactone plays a regulatory role in plant response to abiotic stresses.By eliminating oxidative stress caused by reactive oxygen species,it can potentially increase photosynthetic rate,chlorophyll content,and thus enhance plant drought resistance.Transcriptome studies have explored signal transduction,antioxidant enzyme activity,transcription factors,and expression of stress-and metabolism-related genes induced by extrinsic strigolactone in plants,the effects of strigolactone on plant growth and development have been preliminarily determined,but the studies on inducing crop tolerance to abiotic stresses are still unknown.In this review,the physiological and molecular aspects of the induction of the response to stress in horticultural crops by strigolactone were reviewed.It is important to improve the tolerance and productivity of horticultural crops under abiotic stress.
基金supported by the National Natural Science Foundation of China(Nos.U22A20165 and 52474156)the Key Research and Development Program of Xinjiang Uyghur Autonomous Region(No.2023B01010-1)the China Scholarship Council(No.202406420054).
文摘Water inrush hazards from the floor strata of longwall workingface are commonly encountered in North China coalfields,which essentially result from the evolution of permeability in the floor rock under complex mining-induced stress conditions.Current research rarely addresses the evolution of rock permeability under such complex stress paths.Describing this evolution using only one stress parameter,such as effective stress,deviatoric stress,axial stress,or confining stress,is highly challenging.In this study,we developed a laboratory loading scheme that simulates mining-induced stress evolution.Hydro-mechanical experiments were conducted to investigate the evolution of rock permeability under mining stress.The mechanism on the change of stress-permeability relationships in mining-disturbed rock is revealed,supporting to the analysis of management strategies for floor water-inrush disasters.The results show that rock permeability evolves through four stages,including rapid decline,gradual fluctuation,sharp increase,and slow attenuation.1–2 permeability surges occurred during mining-stress loading,closely linked to the emergence and reversal of deviatoric stress in magnitude and direction.With the first permeability surge,the deviatoric stress within the mudstone reached approximately 1.7 MPa,whereas that of the sandstone was about 1 MPa.The second permeability surge in the mudstone corresponded to the secondary rotation of the principal stress direction.CT and ultrasonic tests suggested an increase in microcracks in both rocks during the first permeability surge.However,the deviatoric stress-permeability plot before and after mining indicated that the fracture of mudstone sample changed significantly,while that of the sandstone remained unchanged.The permeability surges observed at different stages are interpreted as resulting from shear-induced reopening of pre-existing fractures and the formation of new shear-failure fractures.A stress-permeability model jointly governed by effective mean stress and deviatoric stress was established.Furthermore,two strategies are proposed for the floor water-inrush disasters prevention,(i)timely backfilling to reduce deviatoric stress,(ii)grouting after the first permeability surge.This work provides insights into stress-seepage behavior in rocks under complex stress evolution and offers new perspectives for identifying potential water inrush pathways in the floor strata of coal seam during longwall mining.
基金supported by the Zhejiang Provincial Natural Science Outstanding Youth Fund Continuation Project,China(Grant No.LRG25C130002)the Innovation Program of the Chinese Academy of Agricultural Sciences(Grant No.CAAS-CSCB-202402)+3 种基金the Zhejiang Provincial Natural Science Foundation,China(Grant No.LD24C130001)the Biological Breeding-National Science and Technology Major Projects of China(Grant No.2023ZD04066)the Central Public-Interest Scientific Institution Basal Research Fund,China(Grant No.Y2025YC96)the Agricultural Science and Technology Innovation Program,China(Grant No.CAAS-ASTIP-2021-CNRRI).
文摘Prohibitin(PHB)plays critical roles in plant growth and development.In this study,we utilized CRISPR/Cas9 gene-editing technology to generate homozygous OsPHB2 knockout transgenic plants,designated cr-osphb2.The cr-osphb2 line exhibited wider leaves,dwarfism,and shorter panicles.Subcellular localization results indicated that OsPHB2 localizes to mitochondria.Under salt stress conditions,cr-osphb2 exhibited enhanced tolerance.Haplotype(Hap)analysis identified three major Haps(Hap1,Hap2,and Hap3)of OsPHB2,among which Hap2 was associated with a greater number of effective panicles and higher yield,indicating its potential value for breeding applications.Collectively,our findings demonstrate that OsPHB2 plays an important role in regulating growth,development,and salt stress responses in rice.
基金funded by the National Natural Science Foundation of China(32472401).
文摘As a specific spoilage organism of seafood under refrigerated temperature conditions,Shewanella spp.tend to form biofilms that exacerbate the occurrence of seafood spoilage.Biofilm-promoting factor A(BpfA)has been reported to promote the adhesion and biofilm formation of Shewanella spp.,but its role in adhesion and biofilm formation of S.putrefaciens under cold stress needs to be further investigated.To better comprehend the effect of BpfA on adhesion and biofilm formation of S.putrefaciens under cold stress(4℃),bacterial adhesion and biofilm phenotype of S.putrefaciens CN32 WT andΔbpfA at 4℃were analyzed and performed transcriptomics.The results showed that the deletion of bpfA had almost no effect on the growth of S.putrefaciens CN32 at 4℃,but weakened the unicellular adhesion capacity of S.putrefaciens CN32 and destabilized the stability of the multicellular adhesion layer.In addition,the biomass of the mature biofilm formed byΔbpfA was merely around 50%of that observed in the mature biofilm of S.putrefaciens CN32 WT,the average thickness and volume of the biofilm decreased by 18%and 27%,respectively,and the composition of the biofilm changed.Transcriptome analysis demonstrated that the deletion of bpfA led to differential expression of genes involved in metabolic pathways such as bacterial chemotaxis,two-component system,tyrosine metabolism,drug metabolism-other enzymes and biofilm formation-Vibrio cholerae,which in turn influenced bacterial adhesion and biofilm formation.Those results advance our acknowledgment of the character of BpfA on adhesion and biofilm formation of S.putrefaciens CN32,which contributes to understanding bacterial adhesion and the control of biofilm formation.
基金supported by The Program of Science and Technology Development Plan of Jilin Province(20230202066NC)Graduate Innovation Research Project of Jilin University(2024CX151)。
文摘Background Heat stress(HS)can impair boar testicular function,leading to reproductive issues.However,chlorogenic acid(CGA)has been shown to mitigate HS-induced damage in various livestock and poultry species.Prepuberty is an important stage of testicular development in boars after birth.However,the protective effect of CGA on testicular HS injury during prepuberty boars and the underlying mechanisms are still not fully understood.Results In vivo,a total of 30 healthy boars with similar body weights and ages were obtained and randomly divided into 3 groups,which were fed a basal diet supplemented with CGA 0(the ND_TN group),0(the ND_HS group)or 1,000(the CGA_HS group)mg/kg.After being fed for 28 d,all the groups,except the ND_TN group,were treated with high temperature for 7 d,after which samples were collected from the boars and analysed.The results showed that CGA significantly mitigated the HS-induced reduction in T-AOC content in testicular tissue and sperm density.Mechanistically,multiomics analysis revealed that the genes differentially expressed by CGA and HS were predominantly associated with the glutathione metabolism pathway.The combined analysis of transcriptomics and proteomics revealed that only BLVRA was affected by both HS and CGA when the mRNA and protein levels of a gene showed differential expression with the same trend.In vitro studies confirmed that CGA modulated GPX3 expression via BLVRA,affected GPx activity,and attenuated HS-induced ROS accumulation.Conclusions In conclusion,prepubertal HS impairs the spermatogenic capacity of boars.BLVRA may mediate the testicular protective effect of CGA,although in vivo validation of this pathway is needed.This study contributes to elucidating the mechanisms underlying the effects of HS on prepubertal boar testicular development using multiomics approaches,laying a foundation for the potential utilization of CGA in swine production.
基金Fund for Scientific and Technological Research of Argentina(FONCyT PICT 2018 number 03521).
文摘Ceratitis capitata(Wiedemann)is a cosmopolitan pest of economic importance.It is controlled by using the Sterile Insect Technique(SIT),which involves rearing and release of sterile males destined to mate with wild females,causing generation-to-generation suppression.Medflies are colonized by microorganisms,primarily the Enterobacteriaceae,with the genera Klebsiella and Enterobacter being the most common.Such microbiota contributes to host fitness.During the SIT,diet with antibiotics and irradiation for sterility of adults alter microbiota.We aimed to determine the role of Medfly microbiota on resistance to abiotic stress conditions,evaluating its function under:(i)starvation,(ii)elevated temperatures,and(iii)dry environments.These conditions simulate challenges Medfly may encounter after release,which differ from controlled rearing environments.We compared adult survival between symbiotic and aposymbiotic individuals,under starvation,two thermal regimes(25 and 30℃)or two humidity regimes(20%-25%and 80%-90%R.H.).Aposymbiotic individuals were obtained after providing them with water containing a mixture of antibiotics and methylparaben.Treatment with antimicrobials effectively reduced the gut microbiota.While starvation had no significant effect on survival,a higher proportion of aposymbiotic individuals died earlier at 30℃ and under dry humidity,with the effect being more pronounced after 48 h.Our results suggest that microbiota plays a role in adaptation of Medfly under environmental stress.We report for the presence of a culturable yeast in the digestive tract of C.capitata,Zygosaccharomyces rouxii.Providing a probiotic adult diet with bacteria and Z.rouxii prior to release could improve SIT outcomes under adverse conditions.
基金supported by the Research Fund of the National Natural Science Foundation of China(No.52374205)the Fundamental Research Project of the Educational Department of Liaoning Province(No.JYTMS20230793)the Research Fund of the State Key Laboratory of Coal Resources and Safe Mining,CUMT(No.YJY-XD-2024-A-016).
文摘To address the key scientific challenge of monitoring the dynamic fracturing of surrounding rock in deep roadways,this study systematically investigates the quantitative relationship between stress and charge signals during coal mass loading.By integrating innovative analytical approaches,introducing quantitative evaluation indices,and developing a charge–stress inversion model,and incorporating underground monitoring practices,significant progress has been achieved in elucidating the correlation between stress variations and charge signals throughout the entire coal mass fracturing process.First,in the field of stress–charge correlation analysis,empirical mode decomposition(EMD)was combined with wavelet coherence analysis for the first time,enabling the removal of slow-varying stress trends while retaining high-frequency fluctuations.This approach allowed for the quantitative characterization of the evolution of coherence between stress variations and charge fluctuations across multiple time scales.Second,coherence skewness and the proportion of high-coherence intervals were innovatively introduced to examine the influence of time scale selection on correlation results.On this basis,a criterion for determining the near-optimal observation scale of charge signals was proposed,providing a quantitative reference for time scale selection in similar signal analyses.Finally,by correlating charge signals with coal damage factors and stress states,a charge-based damage evolution equation was established to achieve effective stress inversion.Combined with in situ monitoring of stress and charge in roadway surrounding rock,this approach revealed the correlation characteristics of stress and charge intensity responses during the dynamic fracturing process.The results indicate,first,that charge signals are not significantly correlated with the absolute stress level of coal but are directly associated with stress variations following coal damage and failure,with the amplitude of charge fluctuations increasing alongside stress fluctuations.Second,coherence between stress and charge signals varies markedly across time scales,with excessively small or large scales leading to distortion,and the scale corresponding to the peak proportion of intervals with coherence>0.8 was identified as the near-optimal observation scale.Third,charge signals can effectively characterize coal damage factors,and the established damage evolution equation can effectively invert stress variation trends.Fourth,in underground roadways,zones of dynamic fracturing in surrounding rock are commonly located in areas where stress concentration overlaps with regions of high charge intensity,further confirming the strong consistency between charge and stress variations.These findings improve the theoretical framework of charge signal responses in loaded coal and provide a scientific basis for precise“stress-charge”monitoring of dynamic disasters,offering practical potential for engineering applications.
