This study explored the effect of supplementing the diet with n-3 polyunsaturated fatty acids(PUFA)on liver gene expression and its contribution to the recovery of cows during the transition phase.A total of 30 multip...This study explored the effect of supplementing the diet with n-3 polyunsaturated fatty acids(PUFA)on liver gene expression and its contribution to the recovery of cows during the transition phase.A total of 30 multiparous non-lactating Holstein dairy cows(35 d before expected calving)were randomly fed a diet with either 1%dry matter(DM)of hydrogenated fatty acid(FA)(C16:00 enriched;control group[CON])or 3.5%DM of extruding flaxseed(n-3 enriched;HN3 group).The cows underwent a 7-d adaptation period,followed by a 28-d prepartum period and a 28-d postpartum period.In the beginning of the experiment,parity,body weight(BW),body condition score(BCS)and milk yield were 2.6±1.2,757.5±65.8 kg,3.3±0.2 and 10,286.5±1464.8 kg/previous lactation(mean±SD),respectively.The relative abundances of Bacteroidota(P=0.047)and Spirochaetota(P=0.091)were higher and that of Patescibacteria(P=0.076)was lower in the HN3 group than in the CON group on 4 d before prepartum.The dry matter intake(DMI)of cows was positively correlated with the abundance of bacteria in the rumen(Spirochaetota:r=0.871,P<0.001;Bacteroidota:r=0.896,P<0.001)and the differential expression of genes involved in taste transduction(ACSL1:r=0.673,P<0.001;PLIN4:r=0.632,P<0.01;CPT1A:r=0.694,P<0.001).These results suggest that dietary n-3 PUFA at an appropriate concentration can promote DMI recovery by upregulating the expression of these genes and maintaining the balance of the rumen microbiota.展开更多
This study was conducted to investigate potential regulatory mechanisms of feed efficiency(FE)in sheep by linking rumen microbiota with its host by the multi-omics analysis.One hundred and ninety-eight hybrid female s...This study was conducted to investigate potential regulatory mechanisms of feed efficiency(FE)in sheep by linking rumen microbiota with its host by the multi-omics analysis.One hundred and ninety-eight hybrid female sheep(initial body weight=30.88±4.57 kg;4-month-old)were selected as candidate sheep.Each test sheep was fed in an individual pen for 60 days,and the residual feed intake(RFI)was calculated.The ten candidate sheep with the highest RFI were divided into the Low-FE group,and the ten with the lowest RFI were divided into the High-FE group,all selected for sample collection.The RFI,average daily gain and average daily feed intake were highly significantly different between the two experimental groups(P<0.05).Compared with Low-FE group,the insulin-like growth factor-1 and very low-density lipoprotein in serum and the propionate in rumen significantly increased in High-FE group(P<0.01),but the acetate:propionate ratio in rumen significantly decreased in High-FE group(P=0.034).Metagenomics revealed Selenomonas ruminantium,Selenomonas sp.and Faecalibacterium prausnitzii were key bacteria,and increased abundance of the genes encoding the enzymes for cellulose degradation and production of propionate in High-FE group.The results of proteomics and section showed the rumen papilla length and expression of carbonic anhydrase and Na^(+)/K^(+)-ATPase were significantly higher in High-FE group(P<0.05).On the other hand,the acetyl-CoA content significantly increased in the liver of High-FE group(P=0.002).The relative expression levels of insulin-like growth factor-1 and apolipoprotein A4 genes were significantly up-regulated in the liver of High-FE group(P<0.05),but relative expression level of monoacylglycerol O-acyltransferase 3 gene was significantly down-regulated(P=0.037).These findings provide the mechanism by which the collaborative interaction between rumen microbiota fermentation and host uptake and metabolism of fermentation products impacts feed efficiency traits in sheep.展开更多
基金funded by the National Natural Science Foundation of China (32130100)the 2115 Talent Development Program of China Agricultural University
文摘This study explored the effect of supplementing the diet with n-3 polyunsaturated fatty acids(PUFA)on liver gene expression and its contribution to the recovery of cows during the transition phase.A total of 30 multiparous non-lactating Holstein dairy cows(35 d before expected calving)were randomly fed a diet with either 1%dry matter(DM)of hydrogenated fatty acid(FA)(C16:00 enriched;control group[CON])or 3.5%DM of extruding flaxseed(n-3 enriched;HN3 group).The cows underwent a 7-d adaptation period,followed by a 28-d prepartum period and a 28-d postpartum period.In the beginning of the experiment,parity,body weight(BW),body condition score(BCS)and milk yield were 2.6±1.2,757.5±65.8 kg,3.3±0.2 and 10,286.5±1464.8 kg/previous lactation(mean±SD),respectively.The relative abundances of Bacteroidota(P=0.047)and Spirochaetota(P=0.091)were higher and that of Patescibacteria(P=0.076)was lower in the HN3 group than in the CON group on 4 d before prepartum.The dry matter intake(DMI)of cows was positively correlated with the abundance of bacteria in the rumen(Spirochaetota:r=0.871,P<0.001;Bacteroidota:r=0.896,P<0.001)and the differential expression of genes involved in taste transduction(ACSL1:r=0.673,P<0.001;PLIN4:r=0.632,P<0.01;CPT1A:r=0.694,P<0.001).These results suggest that dietary n-3 PUFA at an appropriate concentration can promote DMI recovery by upregulating the expression of these genes and maintaining the balance of the rumen microbiota.
基金supported by the National Key Research and Develo pment Program of China (2022YFD1300201,2021YFD1600704)the Key Research and Development Program of Shaanxi Province (2021ZDLNY05-02)the China Agriculture Research System (CARS-39-12).
文摘This study was conducted to investigate potential regulatory mechanisms of feed efficiency(FE)in sheep by linking rumen microbiota with its host by the multi-omics analysis.One hundred and ninety-eight hybrid female sheep(initial body weight=30.88±4.57 kg;4-month-old)were selected as candidate sheep.Each test sheep was fed in an individual pen for 60 days,and the residual feed intake(RFI)was calculated.The ten candidate sheep with the highest RFI were divided into the Low-FE group,and the ten with the lowest RFI were divided into the High-FE group,all selected for sample collection.The RFI,average daily gain and average daily feed intake were highly significantly different between the two experimental groups(P<0.05).Compared with Low-FE group,the insulin-like growth factor-1 and very low-density lipoprotein in serum and the propionate in rumen significantly increased in High-FE group(P<0.01),but the acetate:propionate ratio in rumen significantly decreased in High-FE group(P=0.034).Metagenomics revealed Selenomonas ruminantium,Selenomonas sp.and Faecalibacterium prausnitzii were key bacteria,and increased abundance of the genes encoding the enzymes for cellulose degradation and production of propionate in High-FE group.The results of proteomics and section showed the rumen papilla length and expression of carbonic anhydrase and Na^(+)/K^(+)-ATPase were significantly higher in High-FE group(P<0.05).On the other hand,the acetyl-CoA content significantly increased in the liver of High-FE group(P=0.002).The relative expression levels of insulin-like growth factor-1 and apolipoprotein A4 genes were significantly up-regulated in the liver of High-FE group(P<0.05),but relative expression level of monoacylglycerol O-acyltransferase 3 gene was significantly down-regulated(P=0.037).These findings provide the mechanism by which the collaborative interaction between rumen microbiota fermentation and host uptake and metabolism of fermentation products impacts feed efficiency traits in sheep.
