Lipp 型油罐的泄漏可分四级:渗漏、滴漏、严重滴漏和流淌。本文认为,造成这些泄漏的原因有八个;影响泄漏程度的因素有七个;检查泄漏的方法主要有四种;泄漏的防治要贯彻“以防为主,防治结合”的方针;要对油罐设计、建造、试压、使用全过...Lipp 型油罐的泄漏可分四级:渗漏、滴漏、严重滴漏和流淌。本文认为,造成这些泄漏的原因有八个;影响泄漏程度的因素有七个;检查泄漏的方法主要有四种;泄漏的防治要贯彻“以防为主,防治结合”的方针;要对油罐设计、建造、试压、使用全过程实行全面质量管理和控制;已发生的泄漏可采取封堵、疏导、焊补等方法进行治理。Lipp 型油罐在我国自1987年试建成功后,陆续推广。这种新型油罐以其容量大、建造速度快、外形新颖美观等特点,受到油厂和粮库职工的欢迎。随着这种新型油罐建造技术的成熟和建造数量的增多,其泄漏的检查与防治技术便成为需要研究总结的课题之一。本文在分析 Lipp 型油罐泄漏的原因及影响泄漏程度的因素之后,结合施工情况, 总结了检查泄漏的方法;指出泄漏的防治要贯彻“以防为主,防治结合”的方针;在设计、建造、试压、使用全过程都要实行全面质量管理与控制;已发生的泄漏可采取疏导、封堵、焊补等方法进行治理。本文可供油罐建造单位、管理部门和使用单位参考。展开更多
On day 3 of gestation, one uterine horn of fe-male pregnant mouse was injected intraluminally with 5 mL 0.1 mg/mL lactacystin, a specific inhibitor of ubiquitin-pro- teasome pathway (UPP), while the contralateral horn...On day 3 of gestation, one uterine horn of fe-male pregnant mouse was injected intraluminally with 5 mL 0.1 mg/mL lactacystin, a specific inhibitor of ubiquitin-pro- teasome pathway (UPP), while the contralateral horn served as control. Animals were sacrificed by cervical dislocation on day 5, 6, 7 of gestation, respectively. Then the number of implanted embryos in each uterine horn was calculated, and the expression of VEGF and its receptors was examined. The data showed that the number of implanted embryos was decreased significantly after treatment with lactacystin. The results of RT-PCR and Western blot indicated that expres-sion of VEGF and its receptors at mRNA and protein levels was significantly decreased in the treated uterus, meanwhile, the expression of HIF-1a (the a subunit of HIF, a transcrip-tional factor of VEGF) was reduced at both mRNA and pro-tein levels. These data suggested that the effect of UPP on VEGF expression was realized through regulating HIF-1a expression. In addition, UPP is likely to take part in the modulation of VEGF receptors expression. These changes may be one of the reasons for the reduction of implanted embryos.展开更多
基金supported by the State Major Basic Research Project(Grant No.G1999055903)the 2002 Knowledge Innovation Project of the Chinese Academy of Sciences
文摘On day 3 of gestation, one uterine horn of fe-male pregnant mouse was injected intraluminally with 5 mL 0.1 mg/mL lactacystin, a specific inhibitor of ubiquitin-pro- teasome pathway (UPP), while the contralateral horn served as control. Animals were sacrificed by cervical dislocation on day 5, 6, 7 of gestation, respectively. Then the number of implanted embryos in each uterine horn was calculated, and the expression of VEGF and its receptors was examined. The data showed that the number of implanted embryos was decreased significantly after treatment with lactacystin. The results of RT-PCR and Western blot indicated that expres-sion of VEGF and its receptors at mRNA and protein levels was significantly decreased in the treated uterus, meanwhile, the expression of HIF-1a (the a subunit of HIF, a transcrip-tional factor of VEGF) was reduced at both mRNA and pro-tein levels. These data suggested that the effect of UPP on VEGF expression was realized through regulating HIF-1a expression. In addition, UPP is likely to take part in the modulation of VEGF receptors expression. These changes may be one of the reasons for the reduction of implanted embryos.
