AIM: To investigate the correlations between lipid metabolism disorder and the occurrence and development of colorectal cancer by monitoring the alterations in lipid levels in cancerous tissue and serum in patients wi...AIM: To investigate the correlations between lipid metabolism disorder and the occurrence and development of colorectal cancer by monitoring the alterations in lipid levels in cancerous tissue and serum in patients with colorectal cancer.展开更多
Background The association of E670G polymorphism in the proprotein convertase subtilisin/kexin type 9 (PCSK9) gene and serum lipid profiles is inconsistent in dif- ferent ethnic groups.Bai Ku Yao is a special subgroup...Background The association of E670G polymorphism in the proprotein convertase subtilisin/kexin type 9 (PCSK9) gene and serum lipid profiles is inconsistent in dif- ferent ethnic groups.Bai Ku Yao is a special subgroup of the Yao minority in China.The present study was undertaken association of PCSK9 E670G polymorphism and several environmental factors with serum lipid levels in the Guangxi Bai Ku Yao and Han populations.Methods A total of 649 subjects of Bai Ku Yao and 646 participants of Han Chinese were randomly selected from our previous stratified randomized cluster samples.Genotyping of the PCSK9 E670G polymorphism was performed by polymerase chain reaction and restriction fragment length polymorphism combined with gel electrophoresis,and then confirmed by direct sequencing. Results The levels of serum total cholesterol(TC),high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C) and apolipoprotein(Apo) AI were lower in Bai Ku Yao than in Han(P【0.01 for all).The frequency of A and G alleles was 98.00%and 2.00%in Bai Ku Yao,and 95.20%and 4.80%in Han(P【0.01);respectively. The frequency of AA,AG and GG genotypes was 95.99%,4.01%and 0%in Bai Ku Yao,and 91.02%, 8.36%and 0.62%in Han(P【0.01);respectively.There were also significant differences in the genotypic and allelic frequencies between n and the ratio of ApoAI to ApoB in Han Chinese but not in Bai Ku Yao were different between the AA and AG/GG genotypes(P【0.05 for all).The G allele carriers had higher serum HDL-C and higher ApoAI to ApoB ratio than the G allele noncarriers.When serum lipid parameters in Han were analyzed according to sex,the G allele carriers had higher serum HDL and ApoAI levels in males (P【0.05),and lower ApoB level and higher ApoAI to ApoB ratio in females(P【0.05 for all).Multiple linear regression analysis showed that serum HDL-C levels were correlated with genotypes in both ethnic groups(P【0.05 each).Serum lipid parameters were also correlated with sex,age,body massindex,alcohol consumption,cigarette smoking,and blood pressure in both ethnic groups(P【0.05-0.001).Conclusions These results suggest that the PCSK9 E670G polymorphism is mainly associated with some serum lipid parameters in the Han population,both gender show different relations to different serum lipid parameters.The G allele carriers might have higher serum lipid profiles than the G allele noncarriers. ormal LDL-C(≤3.20 mmol/L) and high LDL-C subgroups (】 3.20 mmol/L,P【0.01;respectively) in Bai Ku Yao, and between normal ApoB(≤1.14 g/L) and high ApoB subgroups(】 1.14 g/L,P 【 0.01;respectively) in Han.展开更多
Objectives Apolipoprotein(Apo)A5 gene poly-morphisms and alcohol consumption have been associated with increased serum triglyceride(TG)levels,but little is known about their interactions on serum lipid levels.The pres...Objectives Apolipoprotein(Apo)A5 gene poly-morphisms and alcohol consumption have been associated with increased serum triglyceride(TG)levels,but little is known about their interactions on serum lipid levels.The present study was undertaken polymorphismsand alcohol consumption on serum lipid levels.Methods A total of 516 unrelated nondrinkers and 514 drinkers aged 15-89 were randomly selected from our previous stratified randomized cluster samples.Genotyping of the ApoA5was performed by polymerase chain reaction and restriction fragment length polymorphism,and then confirmed by direct sequencing.Interactions of the ApoA5alcohol consumption were assessed by using a cross-product term between genotypes and the aforementioned factor.Results The levels of total cholesterol(TC),TG,high-density lipoprotein cholesterol(HDL-C),ApoA1 and ApoB were higher in drinkers than in nondrinkers(P【0.05-0.001).The genotypic and allelic frequencies of the three single nucleotide polymorphisms(SNPs)were not different between the two groups.The levels of TG in non-drinkers,and TC,TG,low-density lipoprotein cholesterol(LDL-C)and ApoB in drinkers were different among the three-1131T】C genotypes(P【0.05-0.001).The-1131C allele carriers had higher serum TC,TG,LDL-C and ApoB levels than the allele noncarriers.The levels of TG,HDL-C and ApoB in nondrinkers,and TG and HDL-C in drinkers were different between the two c.553G】T genotypes(P【0.05-0.01).The C.553T allele carriers had higher serum TG and ApoB levels,and lower HDL-C levels than the allele noncarriers.Serum lipid levels in nondrinkers were not different among the three c.457G】A genotypes(P【0.05 for all),but the levels of HDL-C,LDL-C,ApoA1 and ApoB in drinkers were different between the GG and GA/AA geno-types(P【0.05-0.001).The C.457A allele carriers had lower serum HDL-C,LDL-C,ApoAl and ApoB levels than the allele noncarriers.We also observed four haplotypes:G-G-T,G-G-C,G-A-T,and T-G-C with frequencies ranging from 0.06 to 0.87,representing 100%of all haplotypes in the both populations.The ApoA5 haplotypes were significantly(P【0.05)associated at the global level with TC,TG,HDL-C,LDL-C,Apo1,and ApoB,even after correction for multiple testing with permutation test.In particular,carriers of haplo-type G-G-C had significantly higher TC,TG,LDL-C,ApoB than noncarriers,whereas carriers of haplotype C-A-T had significantly lower TC,LDL-C,ApoAl and ApoB,and higher HDL-C than noncarriers.Serum TC levels in nondrinkers were correlated with-1131T】C genotype and allele(P【0.05 for each),whereas serum TC,TG and LDL-C levels in drinkers were associated with-1131 T】C and C.553G】T genotypes,or c.457G】A alleles(P【0.05-0.001).Serum lipid parameters were also correlated with several environmental factors in the both groups.Conclusions The differences in serum lipid profiles between the drinkers and nondrinkers might partly result from different interactions of ApoA5 gene polymor phisms and alcohol consumption.genotypes and-1131T】C,c.553G】T and c.457G】A to detect the interactions of the ApoA5 gene.展开更多
Objectives The association of methylenetetrahy-drofolate reductase(MTHFR)gene polymorphism and serum lipid profiles is still controversial in diverse ethnics.Bai Ku Yao is an isolated subgroup of the Yao minority in C...Objectives The association of methylenetetrahy-drofolate reductase(MTHFR)gene polymorphism and serum lipid profiles is still controversial in diverse ethnics.Bai Ku Yao is an isolated subgroup of the Yao minority in China.The aim of the present study was to eveluate the association of MTHFR C677Tpolymorphism and several environmental factors with serum lipid levels in the Guangxi Bai Ku Yao and Han populations.Methods A total of 780 subjects of Bai Ku Yao and 686 participants of Han Chinese were randomly selected from our previous stratified randomized cluster samples.Genotyping of the MTHFR C677T was performed by polymerase chain reaction and restriction fragment length polymorphism combined with gel electrophoresis,and then confirmed by direct sequencing.Results The levels of serum total cholesterol(TC),high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C),apolipoprotein(Apo)AI and ApoB were lower in Bai Ku Yao than in Han(P【0.05-0.001).The frequency of C and T alleles was 77.4%and 22.6%in Bai Ku Yao,and 60.9%and 39.1%in Han(P【0.001);respectively.The frequency of CC,CT and TT genotypes was 58.7%,37.3%and 4.0%in Bai Ku Yao,and 32.6%,56.4%and 11.0%in Han(P【0.001);respectively.The levels of TC and LDL-C in both ethnic groups were significant differences among the three genotypes(P【0.05-0.01).The T allele carriers had higher serum TC and LDL-C levels than the T allele noncarriers.The levels of ApoB in Han were significant differences among the three genotypes(P【0.05).The T allele carriers had higher serum ApoB levels as compared with the T allele noncarriers.The levels of TC,TG and LDL-C in Bai Ku Yao were correlated with genotypes(P【0.05-0.001),whereas the levels of LDL-C in Han were associated with genotypes(P【0.001).Serum lipid parameters were also correlated with sex,age,body massindex,alcohol consumption,cigarette smoking,and blood pressure in the both ethnic groups.Conclusions The differences in serum TC,TG,LDL-C and ApoB levels between the two ethnic groups might partly result from different genotypic and allelic frequencies of the MTHFR C677Tor differentMTHFR gene-enviromental interactions.展开更多
Objectives To investigate prevalence of blood lipid level among 1401 residents aged≥60 years in rong-gui community.Methods 1401 ones(579 males,822 females,(68.12±6.64)years Of total 1503 residents aged≥60 years...Objectives To investigate prevalence of blood lipid level among 1401 residents aged≥60 years in rong-gui community.Methods 1401 ones(579 males,822 females,(68.12±6.64)years Of total 1503 residents aged≥60 years in the 2 villages which were randomly exampled in rong-gui community were investigated.867 ones aged among 60-69 years(61.9%),430 ones aged among 70-79 years(30.7%),104 ones aged≥60 years(7.4%).Investigative items included age,sex,total cholesterol(TC),triglyceride(TG),body height,body weight,waist circumference(WC),hip circumference(HC),fasting plasma glucose(FG),blood pressure,history of past illness,body mass index(BMI),waist hip ratio(WHR),waist height ratio(WHtR).Results(l)Of total 1401 residents,Prevalence of hyperlipemia were 42.8%,female were signifleanfly higher than male(P【0.01),The difference among three age groups were not significant,the combined prevalence of hyperlipemia and borderline hyperlipemia was 80.1%.(2)The FG、BMI、SBP、DBP、WC、WHR、WHtR of residents with hyperlipemia were higher than ones with normal blood lipid level(P【0.01).(3)By Pearson correlation analysis,we found that hyperlipemia was obviously correlated with FG,BMI,WC,WHR,WHtR,SBP,DBP.Conclusions(1)Prevalence of hyperlipemia were 42.8%,female were signifleanfly higher than male.(2)hyperlipemia were obviously correlated with higher FG、BMI、WC、WHR、WHtR、SBP、DBP.展开更多
<b><span style="font-family:Verdana;">Objective</span></b><span style="font-family:Verdana;">:</span><span><span style="font-family:Verdana;"&...<b><span style="font-family:Verdana;">Objective</span></b><span style="font-family:Verdana;">:</span><span><span style="font-family:Verdana;"> To explore the effects of Danqi Yishen Capsule (Danqi Capsule for Tonifying Kidney) on blood lipid levels and hemorheological indicators of hyperlipidemia patients. </span><span><span style="font-family:Verdana;"><b></b></span><b><b><span style="font-family:Verdana;">Methods</span></b></b></span></span><b><span style="font-family:Verdana;">:</span></b><b><span> </span></b><span style="font-family:Verdana;">A total of 96 patients </span><span style="font-family:Verdana;">with</span><span style="font-family:Verdana;"> hyperlipidemia were randomly divided into </span><span style="font-family:Verdana;">the </span><span style="font-family:Verdana;">control group and observation group of 48 cases respectively. The control group was given by simvastatin in oral administration of 10 mg half an hour after dinner, once a day, while the observation group based on the treatment of </span><span style="font-family:Verdana;">the </span><span style="font-family:Verdana;">control group, was orally given Danshen Yishen Capsule of three capsules each time, three times a day. The treatment of both groups lasted for two months. Then the comparison focused on blood lipid levels, hemorheological indicators</span><span style="font-family:Verdana;">,</span><span><span style="font-family:Verdana;"> and adverse reactions between both groups before and after the treatment. </span><span><span style="font-family:Verdana;"><b></b></span><b><b><span style="font-family:Verdana;">Results</span></b></b></span></span><b><span style="font-family:Verdana;">:</span></b><span> </span><span style="font-family:Verdana;">There was no significant difference</span><span><span style="font-family:Verdana;"> in blood lipid levels and hemorheological indicators between both groups before the treatment (<i></i></span><i><i><span style="font-family:Verdana;">P</span></i><span style="font-family:Verdana;"></span></i></span><i><span> </span></i><span><span style="font-family:Verdana;">> 0.05). The symptoms of both groups were significantly improved after the treatment, and the difference before and after the treatment was statistically significant (<i></i></span><i><i><span style="font-family:Verdana;">P</span></i><span style="font-family:Verdana;"></span></i></span><span> </span><span><span style="font-family:Verdana;">< 0.05), and the difference between both groups was also statistically significant (<i></i></span><i><i><span style="font-family:Verdana;">P</span></i><span style="font-family:Verdana;"></span></i></span><span style="font-family:Verdana;"> < 0.05). There was no significant difference in the incidence rate of adverse reactions between both groups (<i></i></span><i><i><span style="font-family:Verdana;">P</span></i><span style="font-family:Verdana;"></span></i><span> </span><span><span style="font-family:Verdana;">> 0.05). </span><span><span style="font-family:Verdana;"><b></b></span><b><b><span style="font-family:Verdana;">Conclusion</span></b></b></span></span><b><span style="font-family:Verdana;">:</span></b><b><span> </span></b><span style="font-family:Verdana;">Danqi Yishen Capsule has a significant value in clinical applications, which can optimize clinical efficacy, improve blood lipid levels and hemorheological indicators of hyperlipidemia patients, as well as reduce the probability of adverse reactions in patients.</span>展开更多
Objectives Bai Ku Yao(White-trousers Yaos)is a special branch of Yao minority in China.They are now living in both Lihu and Baxu villages,Nandan County,Guangxi,China.The population size is about 30,000.The special cus...Objectives Bai Ku Yao(White-trousers Yaos)is a special branch of Yao minority in China.They are now living in both Lihu and Baxu villages,Nandan County,Guangxi,China.The population size is about 30,000.The special customs and culture of Bai Ku Yao,including their special clothing,intra-ethnic marriages and alcohol intake are still completely conserved to the present day.In previous epidemiologic studies,we found that the serum lipid levels and the prevalence of hyperlipidaemia were lower in Bai Ku Yao than in Han Chinese from the same region.This ethnic difference in serum lipid profiles is still not well known.We hypothesized that there may be significant differences in some genetic polymorphismsssociation of low density lipoprotein receptor(LDL-R)genepolymorphism and several environmental factors with serum lipid levels in the Guangxi Bai Ku Yao and Han populations.Methods A total of 1024 subjects of Bai Ku Yao and 792 participants of Han Chinese were stud-ied by a stratified randomized cluster sampling.Epidemiological survey was carried out using internationally standardized methods.Information on demographics,socioeconomic status,and lifestyle factors was collected with standardized questionnaires.The height,weight,waist circumference,blood pressure,and serum total cholesterol(TC),triglyceride(TG),high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C),apolipoprotein(Apo)A1,and ApoB were measured.Body massindex(BMI,kg/m2)was calculated.Genotyping of the LDL-RAvaⅡwas performed by polymerse chain reaction and restriction fragment length polymorphism combined with gel electrophoresis,and then confirmed by direct sequencing.Results(l)The height,weight,serum TC,HDL-C,LDL-C,ApoAl levels and the ratio of ApoAl to ApoB were lower in Bai Ku Yao than in Han Chinese(P【0.01 for all),whereas the percentage of subjects who consumed alcohol or smoked cigarettes was higher in Bai Ku Yao than in Han Chinese(P【0.01 for each).(2)The frequency of A+allele in Bai Ku Yao was 34.5%,and the frequencies of A-A-,A-A+and A+A+genotypes were 42.6%,45.9%and 11.5%;respectively.The frequency of A+allele in Han Chinese was 19.3%(P【0.001),and the frequencies of A-A-,A-A+and A+A+genotypes were 64.9%,31.6%and 3.5%(P【0.001);respectively.The frequencies of A-A-,A-A+and A+A+genotypes in Bai Ku Yao were significant difference between males and females,between normal TC and high TC subgroup,and between normal LDL-C and high LDL-C subgroup(P【0.05 for all),whereas the frequencies of A-and A+?alleles in Han Chinese were significant difference between males and females(P【0.05).(3)Serum LDL-C levels in Bai Ku Yao were significant difference among the A-A-,A-A+and A+A+genotypes(P【0.05),the A+carriers had higher serum LDL-C levels.Serum HDL-C levels in Han Chiese were significant difference among the A-A-,A-A+and A+A+genotypes(P【0.01),the A+carriers had higher serum HDL-C levels.(4)After adjusting other factors,the prevalence of LDL-C abnormality was still higher in Han Chiese than in Bai Ku Yao.The prevalence of TC abnormality in Han Chinese was almost twice high as in Bai Ku Yao.The age and diet were common risk factor for TC abnormality.No effect of AvaⅡgenotype or alcohol consumption on the TC abnormality was found,but the combination of geno-type and alcohol consumption can increase the prevalence of TC abnormality[Exp(B)=(1.154)].Age was negatively cor-related with TG level.Conclusions Serum TC and LDL-C levels were lower in Bai Ku Yao than in Han Chinese.There were significant differences in the AvaⅡallele and genotype frequencies between the he A+carriers in Bai Ku Yao had higher serum LDL-C levels,whereas the A+carriers in Han had higher serum HDL-C levels.Interactions between alcohol consumption or cigarette smoking and the LDL-R AvaⅡgenotype were also observed.The differences in the serum lipid profiles between the two ethnic groups might partly result from different genotypic frequency of LDL-R AvaⅡpolymorphism or differentgene-enviromental interactions.Bai Ku Yao and Han population,the frequency of A+allele was higher in Bai Ku Yao than in Han.T between the two ethnic groups.Therefore,the aim of the present study was to detect the.展开更多
Objectives The association of peroxisome prolif-erator-activated receptor delta(PPARD)+294T】C polymorphism and serum lipid levels is inconsistent in several previous studies.Bai Ku Yao is an isolated association of P...Objectives The association of peroxisome prolif-erator-activated receptor delta(PPARD)+294T】C polymorphism and serum lipid levels is inconsistent in several previous studies.Bai Ku Yao is an isolated association of PPARD+294T】C(rs2016520)polymorphism and several environmental factors with serum lipid levels in the Guangxi Bai Ku Yao and Han populations.Methods A total of 609 subjects of Bai Ku Yao and 573 participants of Han Chinese were randomly selected from our previous stratified randomized cluster samples.Genotyping of the PPARD+294T】C polymorphism was performed by polymerase chain reaction and restriction fragment length polymorphism combined with gel electrophoresis,and then confirmed by direct sequencing.Results The levels of serum total cholesterol(TC),high-density lipoprotein cholesterol(HDL-C),apolipoprotein(Apo)AI and ApoB were lower in Bai Ku Yao than in Han(P【0.001 for all).The frequency of T and C alleles was 77.50%and 22.50%in Bai Ku Yao,and 72.43%and 27.57%in Han(P【0.01);respectively.The frequency of TT,TC and CC genotypes was 60.59%,33.83%and 5.53%in Bai Ku Yao,and 52.18%,40.50%and 7.32%in Han(P【0.05);respectively.The levels of LDL-C,ApoB and the ratio of ApoAI to ApoB in Bai Ku Yao were different among the three genotypes in females but not in males(P【0.05 for all).The subjects with TT and TC genotypes had lower serum LDL-C and ApoB levels and higher the ratio of ApoAI to ApoB than the CC genotype in females.The levels of TC and ApoB in the total Han population were different among the three genotypes(P【0.05 for all).The C allele carriers had higher serum TC and ApoB levels than the C allele noncarriers.When serum lipid levels were analyzed according to sex,the difference in serum TC levels in Han was significant in males(P【0.01)but not in females,whereas the difference in serum ApoB levels was significant in females(P【0.05)but not in males.Serum TC and ApoB levels were correlated with genotypes in Han(P【0.05 for each)but not in Bai Ku Yao.Serum lipid parameters were also correlated with sex,age,body massindex,alcohol consumption,cigarette smoking,and blood pressure in both ethnic groups.Conclusions These results suggest that the association of PPARD+294T】C polymorphism and serum lipid levels is different between the Bai Ku Yao and Han populations.The discrepancy between the two ethnic groups might partly result from different PPARD+294T】C polymorphism or PPARD gene-enviromental interactions,subgroup of the Yao minority in China.展开更多
Objectives Endotheliallipase(EL)is a major determinantof high-density lipoprotein-cholesterol(HDL-C)metabolism,but the association of endothelial lipase gene(LIPG)polymorphism and serum HDL-C levels is scarce and conf...Objectives Endotheliallipase(EL)is a major determinantof high-density lipoprotein-cholesterol(HDL-C)metabolism,but the association of endothelial lipase gene(LIPG)polymorphism and serum HDL-C levels is scarce and conflicting in diverse populations.Bai Ku Yao is an isolated subgroup of the Yao minority in China.This study was designed to detect the association of LIPG 584C】T(rs2000813)polymorphism and several environmental factors with serum lipid levels in the Guangxi Bai Ku Yao and Han populations.Methods A total of 645 subjects of Bai Ku Yao and 638 participants of Han Chinese were randomly selected from our previous stratified randomized cluster samples.Genotyping of the LIPG584C】T was performed by polymerse chain reaction and restriction fragment length polymorphism combined with gel electrophoresis,and then confirmed by direct sequencing.Results The levels of serum total cholesterol(TC),HDL-C,low-density lipoprotein cholesterol(LDL-C)and apolipoprotein(Apo)AI and ApoB were lower in Bai Ku Yao than in Han(P【0.05-0.001).The frequency of C and T alleles was 73.5%and 26.5%in Bai Ku Yao,and 67.9%and 32.1%in Han(P【0.01).The frequency of CC,CT and TT genotypes was 50.4%,46.2%and 3.4%in Bai Ku Yao,and 41.4%,53.1%and 5.5%in Han(P【0.01);respectively.Serum HDL-C levels in both ethnic groups were different among the three genotypes(P【0.05 for each).Serum TC levels in both ethnic groups were also different between CC and CT/TT types(P【0.05 for each).The T allele carriers had higher serum HDL-C and TC levels than the T allele noncarriers.Multivariate logistic regression analysis showed that the levels of HDL-C and ApoB were correlated with genotype in Bai Ku Yao(P【0.05 for each),whereas the levels of TC and HDL-C were associated with genotype in Han Chinese(P【0.05 and P【0.01).Serum lipid parameters were also correlated with gender,age,weight,body massindex,alcohol consumption,cigarette smoking,and blood pressure in the both ethnic groups.Conclusions The frequency of LIPG 584Tallele is lower in the Bai Ku Yao than in the Han populations.The LIPG 584Tallele is associated with serum HDL-C and TC levels.The differences in serum lipid profiles between the two ethnic groups might partly result from different genotypic and allelic of LIPG 584C】T or different LIPG-enviromental interactions.展开更多
Objective To evaluate whether the effects of HMG - CoA reductase inhibitors on patients with hyperlipidemia are closely related to baseline lipid levels. Methods The data analyzed originated from 3 separate multicente...Objective To evaluate whether the effects of HMG - CoA reductase inhibitors on patients with hyperlipidemia are closely related to baseline lipid levels. Methods The data analyzed originated from 3 separate multicenter clinical trials with similar designs during 1994 to 1999. 166 patients with mean age 58. 9±9. 2 years were involved in Simvastatin Clinical Trial with simvastatin 10 mg once daily for 8 weeks. 146 patients with mean age 57. 9±8. 7years were involved in Lovastatin Clinical Trial with lovastatin 20 mg once daily for 8 weeks. 105 patients with mean age 57. 8±9. 3 years were involved in Atorvastatin Clinical Trial with atorvastatin 10 mg once daily for 6 weeks. Baseline total cholesterol (TC) was more than 5. 98 mmol. L - 1, and baseline triglyceride (TG) was less than 4. 52 mmo. L - 1. The patients were grouped by baseline lipid levels. Results The higher the baseline TC, low density lipoprotein cholesterol (LDL - C) and TG levels were, the more effective the simvastatin, lovastatin, or atorvastatin was in reducing serum TC, LDL - C, and TG, respectively. A positive linear correlation was found between baseline values and effects of simvastatin, lovastatin, or atorvastatin in reducing serum TC, LDL - C, and TG, respectively. Conclusion The changes of reduction on serum lipid with HMG - CoA reductase inhibitors in patients with hyperlipidemia were influenced by baseline lipid levels.展开更多
Diabetes mellitus is one of the worldwide largest growing metabolic diseases. In this study the effects of consumption of raw garlic on serum lipid level, blood sugar and a number of effective hormones on lipid and su...Diabetes mellitus is one of the worldwide largest growing metabolic diseases. In this study the effects of consumption of raw garlic on serum lipid level, blood sugar and a number of effective hormones on lipid and sugar metabolism (thyroid hormones and insulin) in individuals either with high level of blood sugar or lipid were investigated. Eighty five volunteers having following clinical history enrolled in the study: Group 1: having blood sugar more than 126 mg/dl (30 vo-lunteers);Group 2: having cholesterol higher than 245 mg/dl (30 volunteers);Group 3: having blood sugar over 126 mg/dl and cholesterol more than 245 mg/dl (25 volunteers). In group 1, there wasn’t a sig-nificant differences between biochemical factors be-fore and after garlic consumption, whereas, in the second group after 6 weeks of garlic consumption a decline in cholesterol (P < 0.001), FBS and TG (P < 0.01) was observed but HDL-C (P < 0.001) was in-creased. Six weeks after termination of garlic con-sumption, cholesterol (P < 0.001), FBS and TG (P < 0.05) were in- creased and HDL-C (P < 0.01) was de-creased. In the third group, total cholesterol (P < 0.001), FBS (P < 0.01) and HbA1c decreased while HDL-C was in- creased. Other factors, including thy-roid hormones (T3, T4), TSH and insulin showed no significant alte- ration. In conclusion our results shown that raw garlic consumption can reduce the FBS and Lipid in hyperglycemic and/or hyperlipidemic individuals.展开更多
Objective To observe the effects of Huashi Tongbi Formula(HTF)on blood lipid levels,endothelial cell function,and gut microbiota in rats with erectile dysfunction(ED).Methods SD rats were randomly divided into blank g...Objective To observe the effects of Huashi Tongbi Formula(HTF)on blood lipid levels,endothelial cell function,and gut microbiota in rats with erectile dysfunction(ED).Methods SD rats were randomly divided into blank group,sham operation group,model group,sildenafil group,and HTF low,medium,and high dose groups,with 7 rats in each group.Hyperlipidemia combined with bilateral internal iliac artery ligation were used to establish ED model.After modeling,the sildenafil group received sildenafil(4.5 mg/kg)by gavage,while the HTF group received low,medium,and high doses(3.24,6.48,12.96 g/kg)of HTF by gavage.The other three groups received an equal volume of physiological saline by gavage.After 6weeks of intervention,the intracavernous pressure(ICP)of the penis and mean carotid artery pressure(MAP)were measured using a multi-channel physiological signal recorder,and the ICP/MAP were calculated,the number of erections in rats was recorded.Serum total cholesterol(TC),high-density lipoprotein cholesterol(HDL-C),and low-density lipoprotein cholesterol(LDLC)were measured using a fully automated biochemical analyzer.ELISA was used to detect the levels of vascular endothelial growth factor(VEGF),endothelin-1(ET-1)in serum,and microplate method was used to detect the level of nitric oxide(NO)in the corpus cavernosum tissue of rats.16 S rDNA sequencing was used to detect the gut microbiota of rats.Results Compared with baseline,the serum LDL-C levels of rats in each dose group of HTF decreased,HDL-C levels increased(P<0.05),the TC levels of rats in the medium dose group of HTF decreased(P<0.05).Compared with the blank group,the model group showed a decrease in number of erections,ICP/MAP,serum HDL-C,and NO levels in the corpus cavernosum tissue of the penis,while serum TC,LDL-C,VEGF,and ET-1 levels increased(P<0.05).Compared with the sham operation group,the model group showed a decrease in number of erections and an increase in serum VEGF and ET-1 levels(P<0.05).Compared with the model group,the sildenafil group showed a decrease in serum ET-1 levels and an increase in NO levels in the corpus cavernosum tissue of the penis(P<0.05).The levels of serum LDL-C,VEGF,and ET-1 in rats of various doses of HTF decreased,while the level of HDL-C increased(P<0.05).The low dose group of HTF had an increase in the level of NO in the corpus cavernosum tissue of the penis,while the medium dose group of HTF had an increase in the number of erections and a decrease in serum TC levels(P<0.05).Compared with the sildenafil group,the serum LDL-C and VEGF levels of rats in each dose group of HTF decreased,the HDL-C levels of rats in the low and high dose groups of HTF increased,and the ET-1 levels of rats in the low dose group of HTF decreased(P<0.05).Analysis of gut microbiota showed that HTF could affect the structure of gut microbiota.Compared with the blank group,the abundance of Butyricicoccus,Ruminococcus,Prevotellaceae_UCG-OOl,and Anaerotruncus increased in the model group,while the abundance of Candidatus_Stoquefichus,Facklamia,Jeotgalicoccus,and Enterococcus decreased(P<0.05).Compared with the model group,the HTF mediumdose groupSshowedan increase inthe abundance of Staphylococcus,Faecalibacterium,Jeotgalicoccus,Halomonas,Negativibacillus,Achromobacter,Subdoliguranulus,Lachnospiraceae UCG-010,Bilophila,and Nosocomicoccus(P<0.05),while Faecalibacterium,Family_XIll_UCG-001,and Lachnospiraceae_UCG-006,Butyricicoccus,unidentified_Ruminococcaceae,Lachnospiraceae_ND3007_group,and Lactococcus decreased(P<0.05).Conclusion The HTF may improve lipid disorders and vascular endothelial cell function in rats via affecting the abundance and diversity of gut microbiota,thereby to enhance erectile function.展开更多
BackgroundThe relationship between lipids and coronary artery disease has been well established. However, this is not the case between lipids and heart failure. Ironically, high lipid levels are associated with better...BackgroundThe relationship between lipids and coronary artery disease has been well established. However, this is not the case between lipids and heart failure. Ironically, high lipid levels are associated with better outcomes in heart failure, but the mechan-isms underlying the phenomenon are not fully understood. This study was performed to test the hypothesis that reduced intestinal lipid absorption due to venous congestion may lead to low lipid levels.MethodsWe collected data of clinical characteristics, echocardio-graph, and lipid profile in 442 unselected patients with congestive heart failure. Correlations between lipid levels[including total cho-lesterol（TCL）, high-density lipoprotein cholesterol（HDL-C）, low-density lipoprotein cholesterol（LDL-C）, and triglycerides（TG）]and right ventricle end diastolic diameter （RVEDD）, left ventricle end diastolic diameter （LVEDD）, right atrium diameter （RA）, left atrium diameter （LA）, or left ventricle ejection fraction （LVEF） were analyzed using Pearson correlation and partial correlation. RVEDD, LVEDD, RA, and LA were indexed to the body surface area.ResultsThere was a significantly inverse correlation between TCL le-vels and RVEDD （r=-0.34,P〈0.001） and RA （r=-0.36,P〈0.001）. Other lipids such as LDL-C, HDL-C, and TG had asimilar inverse correlation with RVEDD and RA. All these correlations remained unchanged after adjusting for age, gender, smoking status, physical activity levels, comorbidities, and medication use.ConclusionsLipid levels were inversely correlated to RVEDD in patients with congestive heart failure; however, because this was an observational study, further investigation is needed to verify our results as wellas identify a causal relationship, if any.展开更多
The effects of different dietary lipid levels on lipid accumulation,inflammatory response,serum bio-chemical index and histological features of intestine and hepatopancreas of O.macrolepis was experimentally evaluated...The effects of different dietary lipid levels on lipid accumulation,inflammatory response,serum bio-chemical index and histological features of intestine and hepatopancreas of O.macrolepis was experimentally evaluated in an eight-weeks study.Fish(initial weight 50.11±2.86 g)were fed with five iso-nitrogenous diets(around 390 g/kg protein)varying with lipid level(5%,7%,9%,11%,13%,being LL5,LL7,LL9,LL11,and LL13 respectively)in triplicates.Results showed that the content of crude lipid in carcass and hepatopancreas were not affected by dietary lipid levels(P>0.05).Serum ALT,TP,HDL-c and MDA,etc.,were not significantly affected by diets(P>0.05),while serum total antioxidant capacity in LL9 and LL11 groups were significantly higher than the other groups(P<0.05).Histological features of hepatopancreas and intestine showed no significant difference among the five diets(P>0.05),while the height of intestine villus showed the higher trend in LL9 compared with other groups.The relative expression of lipid metabolism related genes(pparα,cpt-1α,fas,and hsl)and immune response related genes(tlr 22,nrf 2,tnfα,and il-γ)in the hepatopancreas of fish fed diets differing in their crude lipid levels were not significantly different(P>0.05).The results suggested that a proper dietary lipid level of 9%-11%could maintain higher antioxidant and health status of adult O.macrolepis.展开更多
文摘AIM: To investigate the correlations between lipid metabolism disorder and the occurrence and development of colorectal cancer by monitoring the alterations in lipid levels in cancerous tissue and serum in patients with colorectal cancer.
文摘Background The association of E670G polymorphism in the proprotein convertase subtilisin/kexin type 9 (PCSK9) gene and serum lipid profiles is inconsistent in dif- ferent ethnic groups.Bai Ku Yao is a special subgroup of the Yao minority in China.The present study was undertaken association of PCSK9 E670G polymorphism and several environmental factors with serum lipid levels in the Guangxi Bai Ku Yao and Han populations.Methods A total of 649 subjects of Bai Ku Yao and 646 participants of Han Chinese were randomly selected from our previous stratified randomized cluster samples.Genotyping of the PCSK9 E670G polymorphism was performed by polymerase chain reaction and restriction fragment length polymorphism combined with gel electrophoresis,and then confirmed by direct sequencing. Results The levels of serum total cholesterol(TC),high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C) and apolipoprotein(Apo) AI were lower in Bai Ku Yao than in Han(P【0.01 for all).The frequency of A and G alleles was 98.00%and 2.00%in Bai Ku Yao,and 95.20%and 4.80%in Han(P【0.01);respectively. The frequency of AA,AG and GG genotypes was 95.99%,4.01%and 0%in Bai Ku Yao,and 91.02%, 8.36%and 0.62%in Han(P【0.01);respectively.There were also significant differences in the genotypic and allelic frequencies between n and the ratio of ApoAI to ApoB in Han Chinese but not in Bai Ku Yao were different between the AA and AG/GG genotypes(P【0.05 for all).The G allele carriers had higher serum HDL-C and higher ApoAI to ApoB ratio than the G allele noncarriers.When serum lipid parameters in Han were analyzed according to sex,the G allele carriers had higher serum HDL and ApoAI levels in males (P【0.05),and lower ApoB level and higher ApoAI to ApoB ratio in females(P【0.05 for all).Multiple linear regression analysis showed that serum HDL-C levels were correlated with genotypes in both ethnic groups(P【0.05 each).Serum lipid parameters were also correlated with sex,age,body massindex,alcohol consumption,cigarette smoking,and blood pressure in both ethnic groups(P【0.05-0.001).Conclusions These results suggest that the PCSK9 E670G polymorphism is mainly associated with some serum lipid parameters in the Han population,both gender show different relations to different serum lipid parameters.The G allele carriers might have higher serum lipid profiles than the G allele noncarriers. ormal LDL-C(≤3.20 mmol/L) and high LDL-C subgroups (】 3.20 mmol/L,P【0.01;respectively) in Bai Ku Yao, and between normal ApoB(≤1.14 g/L) and high ApoB subgroups(】 1.14 g/L,P 【 0.01;respectively) in Han.
文摘Objectives Apolipoprotein(Apo)A5 gene poly-morphisms and alcohol consumption have been associated with increased serum triglyceride(TG)levels,but little is known about their interactions on serum lipid levels.The present study was undertaken polymorphismsand alcohol consumption on serum lipid levels.Methods A total of 516 unrelated nondrinkers and 514 drinkers aged 15-89 were randomly selected from our previous stratified randomized cluster samples.Genotyping of the ApoA5was performed by polymerase chain reaction and restriction fragment length polymorphism,and then confirmed by direct sequencing.Interactions of the ApoA5alcohol consumption were assessed by using a cross-product term between genotypes and the aforementioned factor.Results The levels of total cholesterol(TC),TG,high-density lipoprotein cholesterol(HDL-C),ApoA1 and ApoB were higher in drinkers than in nondrinkers(P【0.05-0.001).The genotypic and allelic frequencies of the three single nucleotide polymorphisms(SNPs)were not different between the two groups.The levels of TG in non-drinkers,and TC,TG,low-density lipoprotein cholesterol(LDL-C)and ApoB in drinkers were different among the three-1131T】C genotypes(P【0.05-0.001).The-1131C allele carriers had higher serum TC,TG,LDL-C and ApoB levels than the allele noncarriers.The levels of TG,HDL-C and ApoB in nondrinkers,and TG and HDL-C in drinkers were different between the two c.553G】T genotypes(P【0.05-0.01).The C.553T allele carriers had higher serum TG and ApoB levels,and lower HDL-C levels than the allele noncarriers.Serum lipid levels in nondrinkers were not different among the three c.457G】A genotypes(P【0.05 for all),but the levels of HDL-C,LDL-C,ApoA1 and ApoB in drinkers were different between the GG and GA/AA geno-types(P【0.05-0.001).The C.457A allele carriers had lower serum HDL-C,LDL-C,ApoAl and ApoB levels than the allele noncarriers.We also observed four haplotypes:G-G-T,G-G-C,G-A-T,and T-G-C with frequencies ranging from 0.06 to 0.87,representing 100%of all haplotypes in the both populations.The ApoA5 haplotypes were significantly(P【0.05)associated at the global level with TC,TG,HDL-C,LDL-C,Apo1,and ApoB,even after correction for multiple testing with permutation test.In particular,carriers of haplo-type G-G-C had significantly higher TC,TG,LDL-C,ApoB than noncarriers,whereas carriers of haplotype C-A-T had significantly lower TC,LDL-C,ApoAl and ApoB,and higher HDL-C than noncarriers.Serum TC levels in nondrinkers were correlated with-1131T】C genotype and allele(P【0.05 for each),whereas serum TC,TG and LDL-C levels in drinkers were associated with-1131 T】C and C.553G】T genotypes,or c.457G】A alleles(P【0.05-0.001).Serum lipid parameters were also correlated with several environmental factors in the both groups.Conclusions The differences in serum lipid profiles between the drinkers and nondrinkers might partly result from different interactions of ApoA5 gene polymor phisms and alcohol consumption.genotypes and-1131T】C,c.553G】T and c.457G】A to detect the interactions of the ApoA5 gene.
文摘Objectives The association of methylenetetrahy-drofolate reductase(MTHFR)gene polymorphism and serum lipid profiles is still controversial in diverse ethnics.Bai Ku Yao is an isolated subgroup of the Yao minority in China.The aim of the present study was to eveluate the association of MTHFR C677Tpolymorphism and several environmental factors with serum lipid levels in the Guangxi Bai Ku Yao and Han populations.Methods A total of 780 subjects of Bai Ku Yao and 686 participants of Han Chinese were randomly selected from our previous stratified randomized cluster samples.Genotyping of the MTHFR C677T was performed by polymerase chain reaction and restriction fragment length polymorphism combined with gel electrophoresis,and then confirmed by direct sequencing.Results The levels of serum total cholesterol(TC),high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C),apolipoprotein(Apo)AI and ApoB were lower in Bai Ku Yao than in Han(P【0.05-0.001).The frequency of C and T alleles was 77.4%and 22.6%in Bai Ku Yao,and 60.9%and 39.1%in Han(P【0.001);respectively.The frequency of CC,CT and TT genotypes was 58.7%,37.3%and 4.0%in Bai Ku Yao,and 32.6%,56.4%and 11.0%in Han(P【0.001);respectively.The levels of TC and LDL-C in both ethnic groups were significant differences among the three genotypes(P【0.05-0.01).The T allele carriers had higher serum TC and LDL-C levels than the T allele noncarriers.The levels of ApoB in Han were significant differences among the three genotypes(P【0.05).The T allele carriers had higher serum ApoB levels as compared with the T allele noncarriers.The levels of TC,TG and LDL-C in Bai Ku Yao were correlated with genotypes(P【0.05-0.001),whereas the levels of LDL-C in Han were associated with genotypes(P【0.001).Serum lipid parameters were also correlated with sex,age,body massindex,alcohol consumption,cigarette smoking,and blood pressure in the both ethnic groups.Conclusions The differences in serum TC,TG,LDL-C and ApoB levels between the two ethnic groups might partly result from different genotypic and allelic frequencies of the MTHFR C677Tor differentMTHFR gene-enviromental interactions.
文摘Objectives To investigate prevalence of blood lipid level among 1401 residents aged≥60 years in rong-gui community.Methods 1401 ones(579 males,822 females,(68.12±6.64)years Of total 1503 residents aged≥60 years in the 2 villages which were randomly exampled in rong-gui community were investigated.867 ones aged among 60-69 years(61.9%),430 ones aged among 70-79 years(30.7%),104 ones aged≥60 years(7.4%).Investigative items included age,sex,total cholesterol(TC),triglyceride(TG),body height,body weight,waist circumference(WC),hip circumference(HC),fasting plasma glucose(FG),blood pressure,history of past illness,body mass index(BMI),waist hip ratio(WHR),waist height ratio(WHtR).Results(l)Of total 1401 residents,Prevalence of hyperlipemia were 42.8%,female were signifleanfly higher than male(P【0.01),The difference among three age groups were not significant,the combined prevalence of hyperlipemia and borderline hyperlipemia was 80.1%.(2)The FG、BMI、SBP、DBP、WC、WHR、WHtR of residents with hyperlipemia were higher than ones with normal blood lipid level(P【0.01).(3)By Pearson correlation analysis,we found that hyperlipemia was obviously correlated with FG,BMI,WC,WHR,WHtR,SBP,DBP.Conclusions(1)Prevalence of hyperlipemia were 42.8%,female were signifleanfly higher than male.(2)hyperlipemia were obviously correlated with higher FG、BMI、WC、WHR、WHtR、SBP、DBP.
文摘<b><span style="font-family:Verdana;">Objective</span></b><span style="font-family:Verdana;">:</span><span><span style="font-family:Verdana;"> To explore the effects of Danqi Yishen Capsule (Danqi Capsule for Tonifying Kidney) on blood lipid levels and hemorheological indicators of hyperlipidemia patients. </span><span><span style="font-family:Verdana;"><b></b></span><b><b><span style="font-family:Verdana;">Methods</span></b></b></span></span><b><span style="font-family:Verdana;">:</span></b><b><span> </span></b><span style="font-family:Verdana;">A total of 96 patients </span><span style="font-family:Verdana;">with</span><span style="font-family:Verdana;"> hyperlipidemia were randomly divided into </span><span style="font-family:Verdana;">the </span><span style="font-family:Verdana;">control group and observation group of 48 cases respectively. The control group was given by simvastatin in oral administration of 10 mg half an hour after dinner, once a day, while the observation group based on the treatment of </span><span style="font-family:Verdana;">the </span><span style="font-family:Verdana;">control group, was orally given Danshen Yishen Capsule of three capsules each time, three times a day. The treatment of both groups lasted for two months. Then the comparison focused on blood lipid levels, hemorheological indicators</span><span style="font-family:Verdana;">,</span><span><span style="font-family:Verdana;"> and adverse reactions between both groups before and after the treatment. </span><span><span style="font-family:Verdana;"><b></b></span><b><b><span style="font-family:Verdana;">Results</span></b></b></span></span><b><span style="font-family:Verdana;">:</span></b><span> </span><span style="font-family:Verdana;">There was no significant difference</span><span><span style="font-family:Verdana;"> in blood lipid levels and hemorheological indicators between both groups before the treatment (<i></i></span><i><i><span style="font-family:Verdana;">P</span></i><span style="font-family:Verdana;"></span></i></span><i><span> </span></i><span><span style="font-family:Verdana;">> 0.05). The symptoms of both groups were significantly improved after the treatment, and the difference before and after the treatment was statistically significant (<i></i></span><i><i><span style="font-family:Verdana;">P</span></i><span style="font-family:Verdana;"></span></i></span><span> </span><span><span style="font-family:Verdana;">< 0.05), and the difference between both groups was also statistically significant (<i></i></span><i><i><span style="font-family:Verdana;">P</span></i><span style="font-family:Verdana;"></span></i></span><span style="font-family:Verdana;"> < 0.05). There was no significant difference in the incidence rate of adverse reactions between both groups (<i></i></span><i><i><span style="font-family:Verdana;">P</span></i><span style="font-family:Verdana;"></span></i><span> </span><span><span style="font-family:Verdana;">> 0.05). </span><span><span style="font-family:Verdana;"><b></b></span><b><b><span style="font-family:Verdana;">Conclusion</span></b></b></span></span><b><span style="font-family:Verdana;">:</span></b><b><span> </span></b><span style="font-family:Verdana;">Danqi Yishen Capsule has a significant value in clinical applications, which can optimize clinical efficacy, improve blood lipid levels and hemorheological indicators of hyperlipidemia patients, as well as reduce the probability of adverse reactions in patients.</span>
文摘Objectives Bai Ku Yao(White-trousers Yaos)is a special branch of Yao minority in China.They are now living in both Lihu and Baxu villages,Nandan County,Guangxi,China.The population size is about 30,000.The special customs and culture of Bai Ku Yao,including their special clothing,intra-ethnic marriages and alcohol intake are still completely conserved to the present day.In previous epidemiologic studies,we found that the serum lipid levels and the prevalence of hyperlipidaemia were lower in Bai Ku Yao than in Han Chinese from the same region.This ethnic difference in serum lipid profiles is still not well known.We hypothesized that there may be significant differences in some genetic polymorphismsssociation of low density lipoprotein receptor(LDL-R)genepolymorphism and several environmental factors with serum lipid levels in the Guangxi Bai Ku Yao and Han populations.Methods A total of 1024 subjects of Bai Ku Yao and 792 participants of Han Chinese were stud-ied by a stratified randomized cluster sampling.Epidemiological survey was carried out using internationally standardized methods.Information on demographics,socioeconomic status,and lifestyle factors was collected with standardized questionnaires.The height,weight,waist circumference,blood pressure,and serum total cholesterol(TC),triglyceride(TG),high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C),apolipoprotein(Apo)A1,and ApoB were measured.Body massindex(BMI,kg/m2)was calculated.Genotyping of the LDL-RAvaⅡwas performed by polymerse chain reaction and restriction fragment length polymorphism combined with gel electrophoresis,and then confirmed by direct sequencing.Results(l)The height,weight,serum TC,HDL-C,LDL-C,ApoAl levels and the ratio of ApoAl to ApoB were lower in Bai Ku Yao than in Han Chinese(P【0.01 for all),whereas the percentage of subjects who consumed alcohol or smoked cigarettes was higher in Bai Ku Yao than in Han Chinese(P【0.01 for each).(2)The frequency of A+allele in Bai Ku Yao was 34.5%,and the frequencies of A-A-,A-A+and A+A+genotypes were 42.6%,45.9%and 11.5%;respectively.The frequency of A+allele in Han Chinese was 19.3%(P【0.001),and the frequencies of A-A-,A-A+and A+A+genotypes were 64.9%,31.6%and 3.5%(P【0.001);respectively.The frequencies of A-A-,A-A+and A+A+genotypes in Bai Ku Yao were significant difference between males and females,between normal TC and high TC subgroup,and between normal LDL-C and high LDL-C subgroup(P【0.05 for all),whereas the frequencies of A-and A+?alleles in Han Chinese were significant difference between males and females(P【0.05).(3)Serum LDL-C levels in Bai Ku Yao were significant difference among the A-A-,A-A+and A+A+genotypes(P【0.05),the A+carriers had higher serum LDL-C levels.Serum HDL-C levels in Han Chiese were significant difference among the A-A-,A-A+and A+A+genotypes(P【0.01),the A+carriers had higher serum HDL-C levels.(4)After adjusting other factors,the prevalence of LDL-C abnormality was still higher in Han Chiese than in Bai Ku Yao.The prevalence of TC abnormality in Han Chinese was almost twice high as in Bai Ku Yao.The age and diet were common risk factor for TC abnormality.No effect of AvaⅡgenotype or alcohol consumption on the TC abnormality was found,but the combination of geno-type and alcohol consumption can increase the prevalence of TC abnormality[Exp(B)=(1.154)].Age was negatively cor-related with TG level.Conclusions Serum TC and LDL-C levels were lower in Bai Ku Yao than in Han Chinese.There were significant differences in the AvaⅡallele and genotype frequencies between the he A+carriers in Bai Ku Yao had higher serum LDL-C levels,whereas the A+carriers in Han had higher serum HDL-C levels.Interactions between alcohol consumption or cigarette smoking and the LDL-R AvaⅡgenotype were also observed.The differences in the serum lipid profiles between the two ethnic groups might partly result from different genotypic frequency of LDL-R AvaⅡpolymorphism or differentgene-enviromental interactions.Bai Ku Yao and Han population,the frequency of A+allele was higher in Bai Ku Yao than in Han.T between the two ethnic groups.Therefore,the aim of the present study was to detect the.
文摘Objectives The association of peroxisome prolif-erator-activated receptor delta(PPARD)+294T】C polymorphism and serum lipid levels is inconsistent in several previous studies.Bai Ku Yao is an isolated association of PPARD+294T】C(rs2016520)polymorphism and several environmental factors with serum lipid levels in the Guangxi Bai Ku Yao and Han populations.Methods A total of 609 subjects of Bai Ku Yao and 573 participants of Han Chinese were randomly selected from our previous stratified randomized cluster samples.Genotyping of the PPARD+294T】C polymorphism was performed by polymerase chain reaction and restriction fragment length polymorphism combined with gel electrophoresis,and then confirmed by direct sequencing.Results The levels of serum total cholesterol(TC),high-density lipoprotein cholesterol(HDL-C),apolipoprotein(Apo)AI and ApoB were lower in Bai Ku Yao than in Han(P【0.001 for all).The frequency of T and C alleles was 77.50%and 22.50%in Bai Ku Yao,and 72.43%and 27.57%in Han(P【0.01);respectively.The frequency of TT,TC and CC genotypes was 60.59%,33.83%and 5.53%in Bai Ku Yao,and 52.18%,40.50%and 7.32%in Han(P【0.05);respectively.The levels of LDL-C,ApoB and the ratio of ApoAI to ApoB in Bai Ku Yao were different among the three genotypes in females but not in males(P【0.05 for all).The subjects with TT and TC genotypes had lower serum LDL-C and ApoB levels and higher the ratio of ApoAI to ApoB than the CC genotype in females.The levels of TC and ApoB in the total Han population were different among the three genotypes(P【0.05 for all).The C allele carriers had higher serum TC and ApoB levels than the C allele noncarriers.When serum lipid levels were analyzed according to sex,the difference in serum TC levels in Han was significant in males(P【0.01)but not in females,whereas the difference in serum ApoB levels was significant in females(P【0.05)but not in males.Serum TC and ApoB levels were correlated with genotypes in Han(P【0.05 for each)but not in Bai Ku Yao.Serum lipid parameters were also correlated with sex,age,body massindex,alcohol consumption,cigarette smoking,and blood pressure in both ethnic groups.Conclusions These results suggest that the association of PPARD+294T】C polymorphism and serum lipid levels is different between the Bai Ku Yao and Han populations.The discrepancy between the two ethnic groups might partly result from different PPARD+294T】C polymorphism or PPARD gene-enviromental interactions,subgroup of the Yao minority in China.
文摘Objectives Endotheliallipase(EL)is a major determinantof high-density lipoprotein-cholesterol(HDL-C)metabolism,but the association of endothelial lipase gene(LIPG)polymorphism and serum HDL-C levels is scarce and conflicting in diverse populations.Bai Ku Yao is an isolated subgroup of the Yao minority in China.This study was designed to detect the association of LIPG 584C】T(rs2000813)polymorphism and several environmental factors with serum lipid levels in the Guangxi Bai Ku Yao and Han populations.Methods A total of 645 subjects of Bai Ku Yao and 638 participants of Han Chinese were randomly selected from our previous stratified randomized cluster samples.Genotyping of the LIPG584C】T was performed by polymerse chain reaction and restriction fragment length polymorphism combined with gel electrophoresis,and then confirmed by direct sequencing.Results The levels of serum total cholesterol(TC),HDL-C,low-density lipoprotein cholesterol(LDL-C)and apolipoprotein(Apo)AI and ApoB were lower in Bai Ku Yao than in Han(P【0.05-0.001).The frequency of C and T alleles was 73.5%and 26.5%in Bai Ku Yao,and 67.9%and 32.1%in Han(P【0.01).The frequency of CC,CT and TT genotypes was 50.4%,46.2%and 3.4%in Bai Ku Yao,and 41.4%,53.1%and 5.5%in Han(P【0.01);respectively.Serum HDL-C levels in both ethnic groups were different among the three genotypes(P【0.05 for each).Serum TC levels in both ethnic groups were also different between CC and CT/TT types(P【0.05 for each).The T allele carriers had higher serum HDL-C and TC levels than the T allele noncarriers.Multivariate logistic regression analysis showed that the levels of HDL-C and ApoB were correlated with genotype in Bai Ku Yao(P【0.05 for each),whereas the levels of TC and HDL-C were associated with genotype in Han Chinese(P【0.05 and P【0.01).Serum lipid parameters were also correlated with gender,age,weight,body massindex,alcohol consumption,cigarette smoking,and blood pressure in the both ethnic groups.Conclusions The frequency of LIPG 584Tallele is lower in the Bai Ku Yao than in the Han populations.The LIPG 584Tallele is associated with serum HDL-C and TC levels.The differences in serum lipid profiles between the two ethnic groups might partly result from different genotypic and allelic of LIPG 584C】T or different LIPG-enviromental interactions.
文摘Objective To evaluate whether the effects of HMG - CoA reductase inhibitors on patients with hyperlipidemia are closely related to baseline lipid levels. Methods The data analyzed originated from 3 separate multicenter clinical trials with similar designs during 1994 to 1999. 166 patients with mean age 58. 9±9. 2 years were involved in Simvastatin Clinical Trial with simvastatin 10 mg once daily for 8 weeks. 146 patients with mean age 57. 9±8. 7years were involved in Lovastatin Clinical Trial with lovastatin 20 mg once daily for 8 weeks. 105 patients with mean age 57. 8±9. 3 years were involved in Atorvastatin Clinical Trial with atorvastatin 10 mg once daily for 6 weeks. Baseline total cholesterol (TC) was more than 5. 98 mmol. L - 1, and baseline triglyceride (TG) was less than 4. 52 mmo. L - 1. The patients were grouped by baseline lipid levels. Results The higher the baseline TC, low density lipoprotein cholesterol (LDL - C) and TG levels were, the more effective the simvastatin, lovastatin, or atorvastatin was in reducing serum TC, LDL - C, and TG, respectively. A positive linear correlation was found between baseline values and effects of simvastatin, lovastatin, or atorvastatin in reducing serum TC, LDL - C, and TG, respectively. Conclusion The changes of reduction on serum lipid with HMG - CoA reductase inhibitors in patients with hyperlipidemia were influenced by baseline lipid levels.
文摘Diabetes mellitus is one of the worldwide largest growing metabolic diseases. In this study the effects of consumption of raw garlic on serum lipid level, blood sugar and a number of effective hormones on lipid and sugar metabolism (thyroid hormones and insulin) in individuals either with high level of blood sugar or lipid were investigated. Eighty five volunteers having following clinical history enrolled in the study: Group 1: having blood sugar more than 126 mg/dl (30 vo-lunteers);Group 2: having cholesterol higher than 245 mg/dl (30 volunteers);Group 3: having blood sugar over 126 mg/dl and cholesterol more than 245 mg/dl (25 volunteers). In group 1, there wasn’t a sig-nificant differences between biochemical factors be-fore and after garlic consumption, whereas, in the second group after 6 weeks of garlic consumption a decline in cholesterol (P < 0.001), FBS and TG (P < 0.01) was observed but HDL-C (P < 0.001) was in-creased. Six weeks after termination of garlic con-sumption, cholesterol (P < 0.001), FBS and TG (P < 0.05) were in- creased and HDL-C (P < 0.01) was de-creased. In the third group, total cholesterol (P < 0.001), FBS (P < 0.01) and HbA1c decreased while HDL-C was in- creased. Other factors, including thy-roid hormones (T3, T4), TSH and insulin showed no significant alte- ration. In conclusion our results shown that raw garlic consumption can reduce the FBS and Lipid in hyperglycemic and/or hyperlipidemic individuals.
文摘Objective To observe the effects of Huashi Tongbi Formula(HTF)on blood lipid levels,endothelial cell function,and gut microbiota in rats with erectile dysfunction(ED).Methods SD rats were randomly divided into blank group,sham operation group,model group,sildenafil group,and HTF low,medium,and high dose groups,with 7 rats in each group.Hyperlipidemia combined with bilateral internal iliac artery ligation were used to establish ED model.After modeling,the sildenafil group received sildenafil(4.5 mg/kg)by gavage,while the HTF group received low,medium,and high doses(3.24,6.48,12.96 g/kg)of HTF by gavage.The other three groups received an equal volume of physiological saline by gavage.After 6weeks of intervention,the intracavernous pressure(ICP)of the penis and mean carotid artery pressure(MAP)were measured using a multi-channel physiological signal recorder,and the ICP/MAP were calculated,the number of erections in rats was recorded.Serum total cholesterol(TC),high-density lipoprotein cholesterol(HDL-C),and low-density lipoprotein cholesterol(LDLC)were measured using a fully automated biochemical analyzer.ELISA was used to detect the levels of vascular endothelial growth factor(VEGF),endothelin-1(ET-1)in serum,and microplate method was used to detect the level of nitric oxide(NO)in the corpus cavernosum tissue of rats.16 S rDNA sequencing was used to detect the gut microbiota of rats.Results Compared with baseline,the serum LDL-C levels of rats in each dose group of HTF decreased,HDL-C levels increased(P<0.05),the TC levels of rats in the medium dose group of HTF decreased(P<0.05).Compared with the blank group,the model group showed a decrease in number of erections,ICP/MAP,serum HDL-C,and NO levels in the corpus cavernosum tissue of the penis,while serum TC,LDL-C,VEGF,and ET-1 levels increased(P<0.05).Compared with the sham operation group,the model group showed a decrease in number of erections and an increase in serum VEGF and ET-1 levels(P<0.05).Compared with the model group,the sildenafil group showed a decrease in serum ET-1 levels and an increase in NO levels in the corpus cavernosum tissue of the penis(P<0.05).The levels of serum LDL-C,VEGF,and ET-1 in rats of various doses of HTF decreased,while the level of HDL-C increased(P<0.05).The low dose group of HTF had an increase in the level of NO in the corpus cavernosum tissue of the penis,while the medium dose group of HTF had an increase in the number of erections and a decrease in serum TC levels(P<0.05).Compared with the sildenafil group,the serum LDL-C and VEGF levels of rats in each dose group of HTF decreased,the HDL-C levels of rats in the low and high dose groups of HTF increased,and the ET-1 levels of rats in the low dose group of HTF decreased(P<0.05).Analysis of gut microbiota showed that HTF could affect the structure of gut microbiota.Compared with the blank group,the abundance of Butyricicoccus,Ruminococcus,Prevotellaceae_UCG-OOl,and Anaerotruncus increased in the model group,while the abundance of Candidatus_Stoquefichus,Facklamia,Jeotgalicoccus,and Enterococcus decreased(P<0.05).Compared with the model group,the HTF mediumdose groupSshowedan increase inthe abundance of Staphylococcus,Faecalibacterium,Jeotgalicoccus,Halomonas,Negativibacillus,Achromobacter,Subdoliguranulus,Lachnospiraceae UCG-010,Bilophila,and Nosocomicoccus(P<0.05),while Faecalibacterium,Family_XIll_UCG-001,and Lachnospiraceae_UCG-006,Butyricicoccus,unidentified_Ruminococcaceae,Lachnospiraceae_ND3007_group,and Lactococcus decreased(P<0.05).Conclusion The HTF may improve lipid disorders and vascular endothelial cell function in rats via affecting the abundance and diversity of gut microbiota,thereby to enhance erectile function.
文摘BackgroundThe relationship between lipids and coronary artery disease has been well established. However, this is not the case between lipids and heart failure. Ironically, high lipid levels are associated with better outcomes in heart failure, but the mechan-isms underlying the phenomenon are not fully understood. This study was performed to test the hypothesis that reduced intestinal lipid absorption due to venous congestion may lead to low lipid levels.MethodsWe collected data of clinical characteristics, echocardio-graph, and lipid profile in 442 unselected patients with congestive heart failure. Correlations between lipid levels[including total cho-lesterol（TCL）, high-density lipoprotein cholesterol（HDL-C）, low-density lipoprotein cholesterol（LDL-C）, and triglycerides（TG）]and right ventricle end diastolic diameter （RVEDD）, left ventricle end diastolic diameter （LVEDD）, right atrium diameter （RA）, left atrium diameter （LA）, or left ventricle ejection fraction （LVEF） were analyzed using Pearson correlation and partial correlation. RVEDD, LVEDD, RA, and LA were indexed to the body surface area.ResultsThere was a significantly inverse correlation between TCL le-vels and RVEDD （r=-0.34,P〈0.001） and RA （r=-0.36,P〈0.001）. Other lipids such as LDL-C, HDL-C, and TG had asimilar inverse correlation with RVEDD and RA. All these correlations remained unchanged after adjusting for age, gender, smoking status, physical activity levels, comorbidities, and medication use.ConclusionsLipid levels were inversely correlated to RVEDD in patients with congestive heart failure; however, because this was an observational study, further investigation is needed to verify our results as wellas identify a causal relationship, if any.
基金supported by the National Key R&D Program of China(2019YFD0900200)Special R&D Program Project of Chinese Academy of Se-enriched Industry(2019ZKG-1)+1 种基金Key R&D plan of Shaanxi Province(2018ZDXM-NY-045)Shaanxi Special plan project of technological innovation guidance(2022QFY12-03).
文摘The effects of different dietary lipid levels on lipid accumulation,inflammatory response,serum bio-chemical index and histological features of intestine and hepatopancreas of O.macrolepis was experimentally evaluated in an eight-weeks study.Fish(initial weight 50.11±2.86 g)were fed with five iso-nitrogenous diets(around 390 g/kg protein)varying with lipid level(5%,7%,9%,11%,13%,being LL5,LL7,LL9,LL11,and LL13 respectively)in triplicates.Results showed that the content of crude lipid in carcass and hepatopancreas were not affected by dietary lipid levels(P>0.05).Serum ALT,TP,HDL-c and MDA,etc.,were not significantly affected by diets(P>0.05),while serum total antioxidant capacity in LL9 and LL11 groups were significantly higher than the other groups(P<0.05).Histological features of hepatopancreas and intestine showed no significant difference among the five diets(P>0.05),while the height of intestine villus showed the higher trend in LL9 compared with other groups.The relative expression of lipid metabolism related genes(pparα,cpt-1α,fas,and hsl)and immune response related genes(tlr 22,nrf 2,tnfα,and il-γ)in the hepatopancreas of fish fed diets differing in their crude lipid levels were not significantly different(P>0.05).The results suggested that a proper dietary lipid level of 9%-11%could maintain higher antioxidant and health status of adult O.macrolepis.
