The purpose of this study was to characterize the chemical components of the extract of Solanum Nigrum Linn.(SNL),by LC-MS/MS,and to identify 33 compounds by positive and negative total ion flow maps.Network pharmacol...The purpose of this study was to characterize the chemical components of the extract of Solanum Nigrum Linn.(SNL),by LC-MS/MS,and to identify 33 compounds by positive and negative total ion flow maps.Network pharmacology and molecular docking methods were used to investigate the mechanism of action of SNL against ulcerative colitis(UC).A total of 282 component target genes and 1850 disease target genes were obtained,and 157 cross-targets and 16 core-targets were obtained after crossover.A total of 20 signaling pathways such as anti-inflammatory and anti-apoptotic were obtained by GO analysis and KEGG analysis,respectively.It is possible that the anti UC eff ect can be achieved by regulating proteins such as AKT1,EGFR,NFKB1,JUN,and HSP90AA1.Molecular docking results show that the anti UC active ingredients are well docked with the target protein molecules This study provides a scientific basis for the development and utilization of SNL.展开更多
OBJECTIVE:To determine direct targeting of localized adiposity through Morus alba Linne bark injection based on pharmacology network analysis.METHODS:Male C57BL/6J mice were fed a high-fat diet(HFD)to induce obesity.A...OBJECTIVE:To determine direct targeting of localized adiposity through Morus alba Linne bark injection based on pharmacology network analysis.METHODS:Male C57BL/6J mice were fed a high-fat diet(HFD)to induce obesity.After 6 weeks on HFD,the water extract of Morus alba L.bark(MAB,2 mg/mL)was locally injected into one inguinal fat pad,while saline was injected into the other side,3 times/week for 6 weeks(n=6/group).The water extract of MAB was freeze-dried and then diluted in saline before use.RESULTS:HFD-fed mice treated with local MAB topical injection showed reduced adipocyte weight and size in inguinal fat pads by dual-energy X-ray absorptiometry.No toxicity changes seen in liver,spleen,kidney tissue,or alanine aminotransferase/aspartate aminotransferase levels in serum by MAB injection.Protein levels of phosphorylated insulin receptor substrate-1 and glucose transporter type 4,and mRNA expression of adiponectin,were increased in inguinal adipose tissue injected with MAB locally.Locally MAB injection led to a decrease in glucose-6-phosphatase and phosphoenolpyruvate carboxykinase,linked to gluconeogenesis,while forkhead box protein O1,which regulates these factors,was increased.Moreover,there was an increase in adenosine 5‘-monophosphate-activated protein kinase,related to lipogenesis,as well as elevated levels of hormone-sensitive lipase and fatty acid synthase,both associated with lipolysis.These results support the'insulin signaling pathway'and'regulation of lipolysis in adipocytes'identified in the Kyoto Encyclopedia of Genes and Genomes pathway through network analysis.CONCLUSION:This study suggests that MAB topical injection exhibits localized fat reduction by inhibiting insulin resistance,gluconeogenesis and lipogenesis mediator,while activating lipolysis enzymes within targeted adipose site.展开更多
Alcoholic liver injury is a liver disease caused by excessive alcohol consumption,which can lead to chronic liver disease death.Solanum Nigrum Linn taste bitter,cold,has the effect of clearing heat and detoxification,...Alcoholic liver injury is a liver disease caused by excessive alcohol consumption,which can lead to chronic liver disease death.Solanum Nigrum Linn taste bitter,cold,has the effect of clearing heat and detoxification,promoting blood and detumescence.Solanum Nigrum Linn fruit contains a variety of antioxidant enzymes,can remove the body produced by aerobic metabolism harmful substances.In this paper,a model of alcohol-induced liver injury in C57BL/6 mice was established to evaluate the protective effect of Solanum Nigrum Linn green fruit(SNGF)ethanolic extract on alcohol-induced liver injury.H&E staining and oil red O(ORO)staining showed that hepatic lobules were clearly demarcated,vacuoles were significantly reduced and lipid droplets were reduced in SNGF ethanolic extract treatment group.Serum levels of TC,TG,LDH,TBA,AKP,ALT and AST were decreased in the SNGF ethanolic extract treatment group,and SNGF ethanolic extract could clear reactive oxygen species(ROS)in time.MDA content was signifi cantly decreased after SNGF ethanolic extract treatment,while superoxide dismutase(SOD)and GSH-Px contents were increased after SNGF ethanolic extract treatment.These results suggest that SNGF ethanolic extract has a protective effect on alcohol-induced liver injury.展开更多
[Objective] The experiment aimed to research the photosynthetic characteristics of Calophyllum inophyllum Linn.and the relations between Calophyllum inophyllum Linn. and ecological factors.[Method] The portable photos...[Objective] The experiment aimed to research the photosynthetic characteristics of Calophyllum inophyllum Linn.and the relations between Calophyllum inophyllum Linn. and ecological factors.[Method] The portable photosynthesis system(Li-6400) was used to determine photosynthetic characteristics of 6-year-old Calophyllum inophyllum Linn.[Result] Both the leaf net photosynthetic rate change and diurnal variation of transpiration rate were single peak type.The relations among the leaf net photosynthetic rate of Calophyllum inophyllum Linn.and photosynthetically active radiation,CO2 concentration and other relevant ecological factors(including transpiration rate, stomatal conductance, difference of vapour pressure and leaf temperature) were two quadratic linear relations.[Conclusion] Calophyllum inophyllum Linn. preferred strong light irradiation and high temperature environment and the experiment has provided foundations for ecological and commercial cultivations.展开更多
Greenhouse cultivation in northern China has the characteristic of single mode, and the intercropping mode is not common. Greenhouse intercropping of green asparagus （Asparagus officinalis Linn） and coriander （Cori...Greenhouse cultivation in northern China has the characteristic of single mode, and the intercropping mode is not common. Greenhouse intercropping of green asparagus （Asparagus officinalis Linn） and coriander （Coriandrum sativurn L.） not only could increase vegetable kinds, but also could improve cropping index, and the asparagus growers＇ income could also be improved. This paper introduced the intercropping technique of green asparagus and coriander, so as to provide technical support for large-area extension of greenhouse green asparagus cultivation and break the situation of single mode for .qreenhouse cultivation.展开更多
[ Objective] The aim of this study was to establish the optimum cpSSR-PCR system for Jatropha curcas Linn. [ Method] cpSSR-PCR amplification system for Jatropha curcas Linn influenced by five factors including Taq DNA...[ Objective] The aim of this study was to establish the optimum cpSSR-PCR system for Jatropha curcas Linn. [ Method] cpSSR-PCR amplification system for Jatropha curcas Linn influenced by five factors including Taq DNA polymerase, Mg^2＋ , DNA template, dNTP and primer were optimized from several levels. [ Result] The optimum concentration of 20 μl reaction system was 10 × Buffer, 2.00 mmol/L Mg^2＋ , 2 U/μl Taq DNA polymerase, 0.2 mmol/L dNTP, 0.2 μmol/L primer and 35 ng/μl DNA template. [ Conclusion] The optimum annealing temperature for cpSSR-PCR reaction system is 52 ℃, and the cpSSR reaction system is steady and reproducible.展开更多
Microbial biomass and species in the rhizosphere soil of Mirabilis jalapa（Linn.）（the saline-alkali soil contaminated by total petroleum hydrocarbon（TPH））were studied with the technology of phospholipid fatty ac...Microbial biomass and species in the rhizosphere soil of Mirabilis jalapa（Linn.）（the saline-alkali soil contaminated by total petroleum hydrocarbon（TPH））were studied with the technology of phospholipid fatty acids（PLFAs） analysis,to explore the effects of Mirabilis jalapa（Linn.） growth on the structure characteristics of microbial communities and degradation of TPH in the petroleum-contaminated salinealkali soil.The result showed that compared with the CK soil without Mirabilis jalapa（Linn.）,the kind change rates of PLFAs were 71.4%,69.2% and 33.3% in spring,summer and autumn,respectively,and the degradation of TPH increased by 47.6%,28.3%,and 18.9% in the rhizosphere soil in spring,summer and autumn,respectively.Correlation analysis was used to determine the correlation between the degradation of TPH and the soil microbial communities：77.8% of the microbial PLFAs showed positive correlation（the correlation coefficient r﹥0） with the degradation of TPH,and 55.6% of the PLFAs had high positive correlation with the degradation of TPH with a correlation coefficient r ≥0.8.In addition,the relative contents of SAT and MONO had high correlation with the degradation of TPH in the CK soil,and the correlation coefficients were 0.92 and 0.60,respectively;but in the rhizosphere soil,42.1% of the PLFAs had positive correlation with it,and only21.1% had high positive correlation with the degradation of TPH,the relative contents of TBSAT,MONO and CYCLO had moderate or low positive correlation with the degradation of TPH,and the correlation coefficients were 0.56,0.50 and 0.07 respectively.It was shown that the growth of mirabilis jalapa（Linn.） highly affected the microbial community structure and TPH degradation speed in the rhizosphere soil,providing a theoretical basis for the research on phytoremediation of petroleumcontaminated saline-alkali soil.展开更多
Objective:To investigate mosquitocidal effects of ethanolic extract of(lowers of Tagetes erecta(T.erecta) and its chloroform and petroleum ether soluble fractions against the larvae of Culex quinquefasciatus(Cx.quinqu...Objective:To investigate mosquitocidal effects of ethanolic extract of(lowers of Tagetes erecta(T.erecta) and its chloroform and petroleum ether soluble fractions against the larvae of Culex quinquefasciatus(Cx.quinquefasciatus).Methods:The fresh flowers of T.erecta were extracted in cold with ethanol(5.0 L) and after concentration,the ethanol extract was fractionated with chloroform and petroleum ether to afford a brownish syrupy suspension of ethanol extract(50.0 g),petroleum ether soluble fraction(18.6 g) and chloroform soluble fraction(23.8 g).The larvicidal effect of ethanol extract and their solvent fractions were determined by the standard procedure of WHO against different instars of Cx.quinquefasciatus.Results:Among the tested samples the chloroform soluble fractions showed the highest toxicity and consequently,the lowest LC_(50) values(14.14 μ g/mL,17.06 μ g/mL.36.88 μ g/mL and 75.48 μ g/mL) for all the instars larvae of Cx.quinquefasciatus.The larvae showed comparative tolerance in the course of increasing age and time.Conclusions:It can be concluded that the flowers of T.erecta are very effective natural larvicide and could be useful against Cx.quinquefasciatus.展开更多
We studied gas-exchange, chlorophyll pigments, lipid peroxidation, antioxidant enzymes, and biomass partitioning responses in seedlings of four wolfberry species （Lycium chinense Mill. var. potaninii （Pojark.） A. M...We studied gas-exchange, chlorophyll pigments, lipid peroxidation, antioxidant enzymes, and biomass partitioning responses in seedlings of four wolfberry species （Lycium chinense Mill. var. potaninii （Pojark.） A. M. Lu, Lycium chinense Mill., Lycium barbarum L., and Lycium yunnanense Kuang ＆ A. M. Lu） under four water supply regimes. In all four species, drought affected seedlings in terms of chlorophyll content, net photosynthesis rate （Pn）＇ transpiration rate （E）, and lipid peroxidation. Drought also increased some antioxidant enzyme activities, such as peroxidase （POD）, catalase （CAT）, superoxide dismutase （SOD）, and ascorbate peroxidase （APX）. Significant changes in dry biomass partitioning also occurred in response to water stress. In particular, dry biomass of leaves and fruits decreased significantly. L. chinense Mill. and L. barbarum L. possessed greater drought tolerance and exhibited superior antioxidant processing ability and other related physiological traits compared to the other two species. L. chinense Mill. was the most tolerant to all levels of drought. In contrast,L, yunnanense Kuang ＆ A. M. Lu was more affected by water supply and had the lowest resistance to drought stress. These findings would provide some important information regarding genetic resources for future forest tree improvement in relation to drought tolerance.展开更多
文摘The purpose of this study was to characterize the chemical components of the extract of Solanum Nigrum Linn.(SNL),by LC-MS/MS,and to identify 33 compounds by positive and negative total ion flow maps.Network pharmacology and molecular docking methods were used to investigate the mechanism of action of SNL against ulcerative colitis(UC).A total of 282 component target genes and 1850 disease target genes were obtained,and 157 cross-targets and 16 core-targets were obtained after crossover.A total of 20 signaling pathways such as anti-inflammatory and anti-apoptotic were obtained by GO analysis and KEGG analysis,respectively.It is possible that the anti UC eff ect can be achieved by regulating proteins such as AKT1,EGFR,NFKB1,JUN,and HSP90AA1.Molecular docking results show that the anti UC active ingredients are well docked with the target protein molecules This study provides a scientific basis for the development and utilization of SNL.
基金Supported by Korea Health Technology R&D Project through the National Research Foundation of Korea,funded by the Korean Government(Project Number:NRF-2019R1I1A2A01063598)Undergraduate Research Program of the College of Korean Medicine,Kyung Hee University,Republic of Korea,in 2023(Project Number:2023)。
文摘OBJECTIVE:To determine direct targeting of localized adiposity through Morus alba Linne bark injection based on pharmacology network analysis.METHODS:Male C57BL/6J mice were fed a high-fat diet(HFD)to induce obesity.After 6 weeks on HFD,the water extract of Morus alba L.bark(MAB,2 mg/mL)was locally injected into one inguinal fat pad,while saline was injected into the other side,3 times/week for 6 weeks(n=6/group).The water extract of MAB was freeze-dried and then diluted in saline before use.RESULTS:HFD-fed mice treated with local MAB topical injection showed reduced adipocyte weight and size in inguinal fat pads by dual-energy X-ray absorptiometry.No toxicity changes seen in liver,spleen,kidney tissue,or alanine aminotransferase/aspartate aminotransferase levels in serum by MAB injection.Protein levels of phosphorylated insulin receptor substrate-1 and glucose transporter type 4,and mRNA expression of adiponectin,were increased in inguinal adipose tissue injected with MAB locally.Locally MAB injection led to a decrease in glucose-6-phosphatase and phosphoenolpyruvate carboxykinase,linked to gluconeogenesis,while forkhead box protein O1,which regulates these factors,was increased.Moreover,there was an increase in adenosine 5‘-monophosphate-activated protein kinase,related to lipogenesis,as well as elevated levels of hormone-sensitive lipase and fatty acid synthase,both associated with lipolysis.These results support the'insulin signaling pathway'and'regulation of lipolysis in adipocytes'identified in the Kyoto Encyclopedia of Genes and Genomes pathway through network analysis.CONCLUSION:This study suggests that MAB topical injection exhibits localized fat reduction by inhibiting insulin resistance,gluconeogenesis and lipogenesis mediator,while activating lipolysis enzymes within targeted adipose site.
文摘Alcoholic liver injury is a liver disease caused by excessive alcohol consumption,which can lead to chronic liver disease death.Solanum Nigrum Linn taste bitter,cold,has the effect of clearing heat and detoxification,promoting blood and detumescence.Solanum Nigrum Linn fruit contains a variety of antioxidant enzymes,can remove the body produced by aerobic metabolism harmful substances.In this paper,a model of alcohol-induced liver injury in C57BL/6 mice was established to evaluate the protective effect of Solanum Nigrum Linn green fruit(SNGF)ethanolic extract on alcohol-induced liver injury.H&E staining and oil red O(ORO)staining showed that hepatic lobules were clearly demarcated,vacuoles were significantly reduced and lipid droplets were reduced in SNGF ethanolic extract treatment group.Serum levels of TC,TG,LDH,TBA,AKP,ALT and AST were decreased in the SNGF ethanolic extract treatment group,and SNGF ethanolic extract could clear reactive oxygen species(ROS)in time.MDA content was signifi cantly decreased after SNGF ethanolic extract treatment,while superoxide dismutase(SOD)and GSH-Px contents were increased after SNGF ethanolic extract treatment.These results suggest that SNGF ethanolic extract has a protective effect on alcohol-induced liver injury.
文摘[Objective] The experiment aimed to research the photosynthetic characteristics of Calophyllum inophyllum Linn.and the relations between Calophyllum inophyllum Linn. and ecological factors.[Method] The portable photosynthesis system(Li-6400) was used to determine photosynthetic characteristics of 6-year-old Calophyllum inophyllum Linn.[Result] Both the leaf net photosynthetic rate change and diurnal variation of transpiration rate were single peak type.The relations among the leaf net photosynthetic rate of Calophyllum inophyllum Linn.and photosynthetically active radiation,CO2 concentration and other relevant ecological factors(including transpiration rate, stomatal conductance, difference of vapour pressure and leaf temperature) were two quadratic linear relations.[Conclusion] Calophyllum inophyllum Linn. preferred strong light irradiation and high temperature environment and the experiment has provided foundations for ecological and commercial cultivations.
文摘Greenhouse cultivation in northern China has the characteristic of single mode, and the intercropping mode is not common. Greenhouse intercropping of green asparagus （Asparagus officinalis Linn） and coriander （Coriandrum sativurn L.） not only could increase vegetable kinds, but also could improve cropping index, and the asparagus growers＇ income could also be improved. This paper introduced the intercropping technique of green asparagus and coriander, so as to provide technical support for large-area extension of greenhouse green asparagus cultivation and break the situation of single mode for .qreenhouse cultivation.
基金Supported by National Scientific and Technical Supporting Project ofStudies on Superior Species Selecting and Breeding Technique ofJatropha curcasLinn(2007BAD50B01)~~
文摘[ Objective] The aim of this study was to establish the optimum cpSSR-PCR system for Jatropha curcas Linn. [ Method] cpSSR-PCR amplification system for Jatropha curcas Linn influenced by five factors including Taq DNA polymerase, Mg^2＋ , DNA template, dNTP and primer were optimized from several levels. [ Result] The optimum concentration of 20 μl reaction system was 10 × Buffer, 2.00 mmol/L Mg^2＋ , 2 U/μl Taq DNA polymerase, 0.2 mmol/L dNTP, 0.2 μmol/L primer and 35 ng/μl DNA template. [ Conclusion] The optimum annealing temperature for cpSSR-PCR reaction system is 52 ℃, and the cpSSR reaction system is steady and reproducible.
文摘Microbial biomass and species in the rhizosphere soil of Mirabilis jalapa（Linn.）（the saline-alkali soil contaminated by total petroleum hydrocarbon（TPH））were studied with the technology of phospholipid fatty acids（PLFAs） analysis,to explore the effects of Mirabilis jalapa（Linn.） growth on the structure characteristics of microbial communities and degradation of TPH in the petroleum-contaminated salinealkali soil.The result showed that compared with the CK soil without Mirabilis jalapa（Linn.）,the kind change rates of PLFAs were 71.4%,69.2% and 33.3% in spring,summer and autumn,respectively,and the degradation of TPH increased by 47.6%,28.3%,and 18.9% in the rhizosphere soil in spring,summer and autumn,respectively.Correlation analysis was used to determine the correlation between the degradation of TPH and the soil microbial communities：77.8% of the microbial PLFAs showed positive correlation（the correlation coefficient r﹥0） with the degradation of TPH,and 55.6% of the PLFAs had high positive correlation with the degradation of TPH with a correlation coefficient r ≥0.8.In addition,the relative contents of SAT and MONO had high correlation with the degradation of TPH in the CK soil,and the correlation coefficients were 0.92 and 0.60,respectively;but in the rhizosphere soil,42.1% of the PLFAs had positive correlation with it,and only21.1% had high positive correlation with the degradation of TPH,the relative contents of TBSAT,MONO and CYCLO had moderate or low positive correlation with the degradation of TPH,and the correlation coefficients were 0.56,0.50 and 0.07 respectively.It was shown that the growth of mirabilis jalapa（Linn.） highly affected the microbial community structure and TPH degradation speed in the rhizosphere soil,providing a theoretical basis for the research on phytoremediation of petroleumcontaminated saline-alkali soil.
基金Supported by a grant from Ministry of Science and Technology,Bangladesh(No.BPROM/SHA-9/B-ANI-PRO/2009/324)
文摘Objective:To investigate mosquitocidal effects of ethanolic extract of(lowers of Tagetes erecta(T.erecta) and its chloroform and petroleum ether soluble fractions against the larvae of Culex quinquefasciatus(Cx.quinquefasciatus).Methods:The fresh flowers of T.erecta were extracted in cold with ethanol(5.0 L) and after concentration,the ethanol extract was fractionated with chloroform and petroleum ether to afford a brownish syrupy suspension of ethanol extract(50.0 g),petroleum ether soluble fraction(18.6 g) and chloroform soluble fraction(23.8 g).The larvicidal effect of ethanol extract and their solvent fractions were determined by the standard procedure of WHO against different instars of Cx.quinquefasciatus.Results:Among the tested samples the chloroform soluble fractions showed the highest toxicity and consequently,the lowest LC_(50) values(14.14 μ g/mL,17.06 μ g/mL.36.88 μ g/mL and 75.48 μ g/mL) for all the instars larvae of Cx.quinquefasciatus.The larvae showed comparative tolerance in the course of increasing age and time.Conclusions:It can be concluded that the flowers of T.erecta are very effective natural larvicide and could be useful against Cx.quinquefasciatus.
基金supported by the National Natural Science Foundation of China (31360191, 31660220)the Natural Science Foundation of Ningxia Hui Autonomous Region, China (NZ16121)the Self-option and Foundation of Ningxia Academy of Agriculture and Forestry Sciences, China (YES-16-0402, NKYZ-16-0402)
文摘We studied gas-exchange, chlorophyll pigments, lipid peroxidation, antioxidant enzymes, and biomass partitioning responses in seedlings of four wolfberry species （Lycium chinense Mill. var. potaninii （Pojark.） A. M. Lu, Lycium chinense Mill., Lycium barbarum L., and Lycium yunnanense Kuang ＆ A. M. Lu） under four water supply regimes. In all four species, drought affected seedlings in terms of chlorophyll content, net photosynthesis rate （Pn）＇ transpiration rate （E）, and lipid peroxidation. Drought also increased some antioxidant enzyme activities, such as peroxidase （POD）, catalase （CAT）, superoxide dismutase （SOD）, and ascorbate peroxidase （APX）. Significant changes in dry biomass partitioning also occurred in response to water stress. In particular, dry biomass of leaves and fruits decreased significantly. L. chinense Mill. and L. barbarum L. possessed greater drought tolerance and exhibited superior antioxidant processing ability and other related physiological traits compared to the other two species. L. chinense Mill. was the most tolerant to all levels of drought. In contrast,L, yunnanense Kuang ＆ A. M. Lu was more affected by water supply and had the lowest resistance to drought stress. These findings would provide some important information regarding genetic resources for future forest tree improvement in relation to drought tolerance.
