Small datasets are often challenging due to their limited sample size.This research introduces a novel solution to these problems:average linkage virtual sample generation(ALVSG).ALVSG leverages the underlying data st...Small datasets are often challenging due to their limited sample size.This research introduces a novel solution to these problems:average linkage virtual sample generation(ALVSG).ALVSG leverages the underlying data structure to create virtual samples,which can be used to augment the original dataset.The ALVSG process consists of two steps.First,an average-linkage clustering technique is applied to the dataset to create a dendrogram.The dendrogram represents the hierarchical structure of the dataset,with each merging operation regarded as a linkage.Next,the linkages are combined into an average-based dataset,which serves as a new representation of the dataset.The second step in the ALVSG process involves generating virtual samples using the average-based dataset.The research project generates a set of 100 virtual samples by uniformly distributing them within the provided boundary.These virtual samples are then added to the original dataset,creating a more extensive dataset with improved generalization performance.The efficacy of the ALVSG approach is validated through resampling experiments and t-tests conducted on two small real-world datasets.The experiments are conducted on three forecasting models:the support vector machine for regression(SVR),the deep learning model(DL),and XGBoost.The results show that the ALVSG approach outperforms the baseline methods in terms of mean square error(MSE),root mean square error(RMSE),and mean absolute error(MAE).展开更多
Against the backdrop of continuous social development and growing public health demands,the efficiency and scientific nature of the emergency care system are of paramount importance.This paper focuses on researching t...Against the backdrop of continuous social development and growing public health demands,the efficiency and scientific nature of the emergency care system are of paramount importance.This paper focuses on researching the construction of an emergency care system based on the concept of“linkage”,delving into its theoretical foundations,exploring innovative construction models,and analyzing practical cases.The study indicates that an emergency care system under the“linkage”concept can effectively integrate resources and enhance efficiency,providing new insights for improving the construction of the emergency care system.It aims to promote the development of the emergency care system towards a more scientific,efficient,and collaborative direction.展开更多
Background Multibreed genomic prediction(MBGP)is crucial for improving prediction accuracy for breeds with small populations,for which limited data are often available.Recent studies have demonstrated that partitionin...Background Multibreed genomic prediction(MBGP)is crucial for improving prediction accuracy for breeds with small populations,for which limited data are often available.Recent studies have demonstrated that partitioning the genome into nonoverlapping blocks to model heterogeneous genetic(co)variance in multitrait models can achieve higher joint prediction accuracy.However,the block partitioning method,a key factor influencing model performance,has not been extensively explored.Results We introduce mbBayesABLD,a novel Bayesian MBGP model that partitions each chromosome into nonoverlapping blocks on the basis of linkage disequilibrium(LD)patterns.In this model,marker effects within each block are assumed to follow normal distributions with block-specific parameters.We employ simulated data as well as empirical datasets from pigs and beans to assess genomic prediction accuracy across different models using cross-validation.The results demonstrate that mbBayesABLD significantly outperforms conventional MBGP models,such as GBLUP and BayesR.For the meat marbling score trait in pigs,compared with GBLUP,which does not account for heterogeneous genetic(co)variance,mbBayesABLD improves the prediction accuracy for the small-population breed Landrace by 15.6%.Furthermore,our findings indicate that a moderate level of similarity in LD patterns between breeds(with an average correlation of 0.6)is sufficient to improve the prediction accuracy of the target breed.Conclusions This study presents a novel LD block-based approach for multibreed genomic prediction.Our work provides a practical tool for livestock breeding programs and offers new insights into leveraging genetic diversity across breeds for improved genomic prediction.展开更多
Promoting the deep integration of advanced manufacturing and producer services is not only a key path for metropolitan to climb the global value chain,but also an important new driving force for optimizing urban spati...Promoting the deep integration of advanced manufacturing and producer services is not only a key path for metropolitan to climb the global value chain,but also an important new driving force for optimizing urban spatial structure.Based on the two dimen-sions of industrial linkage and spatial co-agglomeration from the perspective of industrial integration,taking Beijing,China as a case,using industrial input-output data,micro-enterprise spatial data,and methods such as industrial linkage model,standard deviation el-lipse,and spatial similarity coefficient,this study explores the coupling relationship between industrial linkage and spatial co-agglomer-ation of advanced manufacturing and producer services from 2007 to 2020.The results demonstrate that:1)the industrial linkage between advanced manufacturing and producer services in Beijing has an upward trend,in which the business services have the most significant improvement,in which the business services have the most improvement while the technology services have the most de-crease,but in general,the industrial linkage between the two industries shows a low level.2)Producer services were more spatially clustered than advanced manufacturing,and the degree of spatial co-agglomeration of the two also showed an increasing trend,with strong regional and industrial heterogeneity characteristics,among which the pairing industry involving commerce services and techno-logy services had a more obvious improvement.3)The coupling analysis found that the spatial dependence between advanced manufac-turing and producer services decreased,showing a weak positive correlation and a decreasing trend between industrial linkage and spa-tial co-agglomeration;the heterogeneity among subdivision pairs is prominent,and the strong correlation between industries does not mean that there is an inevitable spatial co-agglomeration.4)Based on the differences in coupling relationships and influence mechan-isms,four coupling modes are identified:H-H type,H-L type,L-L type,and L-H type.Finally,this study proposes three policy implica-tions for the integration of advanced manufacturing and producer services in metropolitan areas.展开更多
Objective:To investigate the effects of performance-linked early rehabilitation management model on the recovery of neurological function,motor function and activities of daily living in patients after intracerebral h...Objective:To investigate the effects of performance-linked early rehabilitation management model on the recovery of neurological function,motor function and activities of daily living in patients after intracerebral hemorrhage surgery.Methods:A randomized controlled trial design was adopted.A total of 120 patients after cerebral hemorrhage surgery admitted to our hospital from June 2023 to June 2024 were selected as the research subjects and were randomly divided into the experimental group and the control group,with 60 cases in each group according to the random number table method.The control group received routine postoperative care and rehabilitation guidance in neurosurgery,while the experimental group implemented an efficacy linkage early rehabilitation management model on the basis of routine care.Before the intervention,4 weeks after the intervention,and 12 weeks after the intervention,the National Institutes of Health Stroke Scale(NIHSS),the Fugl-Meyer Assessment of Motor Function(FMA),and the Barthel Index(BI)were used to assess the degree of neurological deficits,motor function,and activities of daily living in patients,respectively.Results:After 4 weeks and 12 weeks of intervention,the NIHSS scores of the experimental group[(8.5±2.1)points,(5.2±1.8)points]were significantly lower than those of the control group[(10.8±2.4)points,(7.9±2.2)points](P<0.01);FMA scores[(58.4±10.2)points,(78.6±12.4)points]and BI scores[(55.3±11.5)points,(72.8±13.2)points]were significantly higher than those of the control group[(45.2±9.8)points,(62.3±11.7)points;(42.1±10.6)points,(58.4±12.5)points](P<0.01).Conclusion:The performance-linked early rehabilitation management model can effectively promote the recovery of neurological and motor functions in patients after cerebral hemorrhage surgery and significantly improve their ability to take care of themselves in daily living.It is an efficient rehabilitation management strategy.展开更多
Consecutive stresses,such as initial submergence during germination followed by water deficit during the seedling stage,pose significant challenges to direct-seeded rice cultivation.By Linkage disequilibrium analysis,...Consecutive stresses,such as initial submergence during germination followed by water deficit during the seedling stage,pose significant challenges to direct-seeded rice cultivation.By Linkage disequilibrium analysis,Sub1 and Dro1(Δbp:10 Mb),as well as Sub1 and TPP7(Δbp:6 Mb)were identified to exhibit long-range linkage disequilibrium(LRLD).Meta-QTL analysis further revealed that Sub1 and TPP7 co-segregated for tolerance to submergence at the germination and seedling stages.Based on this,we hypothesized that LRLD might influence plant responses to consecutive stresses.To test this hypothesis,we developed a structured recombinant inbred line population from a cross between Bhalum 2 and Nagina 22,with alleles(Sub1 and TPP7)in linkage equilibrium.Mendelian randomization analysis validated that the parental alleles,rather than the recombinant alleles of Sub1 and TPP7,significantly influenced 13 out of 41 traits under consecutive stress conditions.Additionally,16 minor additive effect QTLs were detected between the genomic regions,spanning Sub1 and TPP7 for various traits.A single allele difference between these genomic regions enhanced crown root number,root dry weight,and specific root area by 11.45%,15.69%,and 33.15%,respectively,under flooded germination conditions.Candidate gene analysis identified WAK79 and MRLK59 as regulators of stress responses during flooded germination,recovery,and subsequent water deficit conditions.These findings highlight the critical role of parental allele combinations and genomic regions between Sub1 and TPP7 in regulating the stress responses under consecutive stresses.Favourable haplotypes derived from these alleles can be utilized to improve stress resilience in direct-seeded rice.展开更多
Objective:To address the problems of disconnection between teaching and practice,weak clinical thinking,and poor post adaptability of students in traditional internal medicine nursing teaching,explore the“dual-teache...Objective:To address the problems of disconnection between teaching and practice,weak clinical thinking,and poor post adaptability of students in traditional internal medicine nursing teaching,explore the“dual-teacher linkage”collaborative teaching path,and improve teaching quality and students’post adaptability.Methods:Two classes of nursing majors in Grade 2023 of our college were selected as research objects.The experimental group(100 students)adopted the“dual-teacher linkage”model(on-campus teachers+clinical experts)to build a“pre-class co-research–in-class coguidance–post-class co-evaluation”system;the control group(98 students)adopted traditional teaching.Comparisons were made after one semester of practice.Results:The average score of the comprehensive assessment in the experimental group was 85.6 points(76.3 points in the control group,p<0.05).The clinical decision-making ability,program improvement ability,and post competence evaluation of the experimental group were all superior to those of the control group,and 93.3%of students were satisfied with this model.Conclusion:The“dual-teacher linkage”model can organically unify knowledge transmission and ability training,and is an effective and innovative practice to deepen the reform of internal medicine nursing teaching and cultivate high-quality skilled nursing talents.展开更多
Covalent organic frameworks(COFs)play a crucial role in metal-free electrocatalysts for promoting oxygen reduction reaction(ORR)due to their adjustable skeleton structure and catalytic activity.While it is true that n...Covalent organic frameworks(COFs)play a crucial role in metal-free electrocatalysts for promoting oxygen reduction reaction(ORR)due to their adjustable skeleton structure and catalytic activity.While it is true that numerous studies have explored COFs for ORR,the critical gap by providing a systematic framework for ligand-driven electronic state manipulation is essential for designing highly active COF-based ORR catalysts.Herein,a series of COF-based metal-free materials have been conceived and synthesized by linkage-engineered strategy with dicarboxaldehyde(BPC),bipyridine-dicarbaldehyde(BPA)and benzodithiophene(BDA)as electronic linkages.Consequently,by incorporating different linkages into COFs,the surface area,electronic state,hydrophobic properties and affinities towards intermediates are optimized.Notably,the benzodithiophene-linked COF(denoted as BDA-COF)has greater catalytic ability with a half-wave potential of 0.74 V vs.RHE and an onset potential of 0.86 V vs.RHE than dicarboxaldehyde(denoted as BPC-COF)and dicarbaldehyde(denoted as BPA-COF).Relevant characterizations,in situ techniques and theoretical calculations confirm that thiophene-S-based COF promotes the electronic migration and enhances the interaction with the intermediate.The result provides insight into for illustration of a high-performance COF-based electrocatalyst via a linkage-engineered approach.展开更多
Artificial photosynthesis of hydrogen peroxide(H_(2)O_(2))using covalent organic frameworks(COFs)as photocatalysts holds promise for future applications.However,the influence of linkage chemistry on the photoelectroch...Artificial photosynthesis of hydrogen peroxide(H_(2)O_(2))using covalent organic frameworks(COFs)as photocatalysts holds promise for future applications.However,the influence of linkage chemistry on the photoelectrochemical properties and photocatalytic performance of COFs remains a significant challenge.Herein,we designed and synthesized a model system with different linkages,including imine-,amine-,azo-linked COFs,then investigated their photocatalytic activity of overall H_(2)O_(2)production.The photocatalytic results revealed varying activities for H_(2)O_(2)synthesis among these COFs,with the azo-linked TTA-Azo-COF(COF synthesized by 4,4’,4’’-(1,3,5-triazine-2,4,6-triyl)-trianiline and terephthalaldehyde)demonstrating the highest overall H2O2 photosynthesis activity of 2516μmol g^(–1)h^(–1)in an O2 atmosphere without any sacrificial agents,which is 6.72 and 2.85 times higher than that of imine-linked TTA-COF and amine-linked TTA-COF-AR,respectively.Furthermore,TTA-Azo-COF maintained a high photosynthesis H2O2 activity of 2116μmol g^(–1)h^(–1)under an air atmosphere,outperforming most COF-based photocatalytic systems under similar reaction conditions.Further characterizations and density functional theory calculations reveal these various linkages in different COFs result in distinct visible-light absorption,charge transfer capacities and formation energy barriers of key intermediates.This work revealed the significant impact of linkages on COFs and provided comprehensive guidance for the rational design of COFs with tailored linkages to fulfill specific requirements for future applications.展开更多
The utilization of covalent organic frameworks(COFs)holds great potential for achieving tailorable tuning of catalytic performance through bottom-up modulation of the reticular structure.In this work,we show that a si...The utilization of covalent organic frameworks(COFs)holds great potential for achieving tailorable tuning of catalytic performance through bottom-up modulation of the reticular structure.In this work,we show that a single-point structural alteration in the linkage within a nickel phthalocyanine(NiPc)-based series effectively modulates the catalytic performance of the COFs in electrochemical CO_(2)reduction reaction(CO_(2)RR).A Ni Pc-based COF series with three members which possess the same Ni Pc unit but different linkages,including piperazine,dioxin,and dithiine,have been constructed by nucleophilic aromatic substitution reaction between octafluorophthalocyanine nickel and tetrasubstituted benzene linkers with different bridging groups.Among these COFs,the dioxin-linked COF showed the best activity of CO_(2)RR with a current density of CO(j_(CO))=-27.99 m A cm^(-2)at-1.0 V(versus reversible hydrogen electrode,RHE),while the COF with piperazine linkage demonstrated an excellent selectivity of Faradaic efficiency for CO(FECO)up to 90.7%at a pretty low overpotential of 0.39 V.In addition,both a high FECO value close to 100%and a reasonable jCO of-8.20 m A cm^(-2)at the potential of-0.8 V(versus RHE)were obtained by the piperazine-linked COF,making it one of the most competitive candidates among COF-based materials.Mechanistic studies exhibited that single-point structural alteration could tailor the electron density in Ni sites and alter the interaction between the active sites and the key intermediates adsorbed and desorbed,thereby tuning the electrochemical performance during CO_(2)RR process.展开更多
The ASEAN-China(Beijing Municipal Administrative Centre)Trade and Investment Promotion Conference serves as a new platform for fostering collaboration in trade,investment,and sustainable growth.The event,held in Tongz...The ASEAN-China(Beijing Municipal Administrative Centre)Trade and Investment Promotion Conference serves as a new platform for fostering collaboration in trade,investment,and sustainable growth.The event,held in Tongzhou District-the municipal administrative center of Beijing-highlights shared aspirations for building an environmentally friendly and innovation-driven regional economy.展开更多
Using VAR-DCC-GARCH model,the literature on commodity price was extended by exploring the co-movement between Chinese nonferrous metal prices and global nonferrous metal prices represented by the nonferrous metal pric...Using VAR-DCC-GARCH model,the literature on commodity price was extended by exploring the co-movement between Chinese nonferrous metal prices and global nonferrous metal prices represented by the nonferrous metal prices from London Metal Exchange(LME).The results show that LME nonferrous metals prices still have a greater impact on Chinese nonferrous metals prices.However,the impact of Chinese nonferrous metals prices on LME nonferrous metals prices is still weak except for lead price.The co-movement of nonferrous metal prices between LME and China presents hysteretic nature,and it lasts for 7-8trading days.Furthermore,the co-movement between LME nonferrous metals prices and Chinese nonferrous metals prices has the characteristics of time-varying,and the correlation of lead prices between LME and China is the more stable than all other nonferrous metals prices.展开更多
In this study, we reported the repaid construction of a molecular marker linkage map of rice (Oryza sativa L.). An F-2 population from the cross between Annong S-1 and Nanjing 11 was used to construct a genetic linkag...In this study, we reported the repaid construction of a molecular marker linkage map of rice (Oryza sativa L.). An F-2 population from the cross between Annong S-1 and Nanjing 11 was used to construct a genetic linkage map of rice. Total of 142 newly screened AFLP markers and 30 anchor markers (25 SSR markers and 5 RFLP markers) were mapped on the 12 chromosomes covering 1537.4 cM of rice genome. The average interval between these markers was 9.0 cM. The total work which usually was finished in more than one year was finished within only 3 months by one person. This is the first plant AFLP map developed in China. A new thermosensitive genic male sterile gene in rice, tms5, was Egged and mapped onto chromosome 2 during the development of the linkage map.展开更多
Prader-Willi Syndrome （PWS） is a genetic disorder that is difficult to detect, particularly at an early age. PWS is caused by disruption of normal, epigenetically controlled gene function in the chromosome 15q11-q13...Prader-Willi Syndrome （PWS） is a genetic disorder that is difficult to detect, particularly at an early age. PWS is caused by disruption of normal, epigenetically controlled gene function in the chromosome 15q11-q13 region. Clinical symptoms are difficult to diagnose in infants and only become clearer at later ages as the patients develop hyperphagia and morbid obesity. Molecular genetic tests are able to definitively diagnose PWS and allow early diagnosis of the syndrome. High resolution cytogenetic testing, methylation-specific PCR （MS-PCR）, and linkage analysis are routinely used to diagnose PWS. To establish a linkage analysis method for Chinese patients, this study identified a useful set of STR markers in the typical PWS deletion and adjacent area, for linkage analysis in two Chinese families with PWS offspring. Using this method, the authors confn＇rned that one patient had a paternal deletion in chromosome 15q 11-q 13 and the other patient had maternal uniparental heterodisomy of chromosome 15. MS -PCR and high resolution chromosome G-banding also confirmed this diagnosis. This linkage analysis method can detect both deletion and uniparental disomy, thus providing valuable information for genetic counseling and the opportunity to analyze the relationship between the genotype and phenotype of PWS.展开更多
[Objective]The aim was to discuss elimination mechanism and equilibrium model about complete linkage gene of panmixis based on fitness.[Method]A mathematical model that gsgenerational evalution of frequency of panmixi...[Objective]The aim was to discuss elimination mechanism and equilibrium model about complete linkage gene of panmixis based on fitness.[Method]A mathematical model that gsgenerational evalution of frequency of panmixis two pairs of complete linkage gene under difference fitness was built by using difference equations to study elimination mechanism and genetic equilibrium state of panncticcoinplete linkage gene.[Result]The panmixis is different from Hardy-Weinberg Equilibrium which reached the equilibrium just by one generation.A method that by using linear equation to calculate the frequency of four gametes and the fitnessed of panmixis was established after detormining is the fitness of 10 genotypes the necessary and sufficient condition of the panmixis to reach balance.[Conclusion]The method is valuable for reference on linkage disequilibrium and hitchhiking effect study.展开更多
In this study, an RIL （recombinant inbred line） population containing 240 lines was developed by single seed descent method （SSD） based on a parent com- bination of small-grain indica cultivar Kasalath and large-g...In this study, an RIL （recombinant inbred line） population containing 240 lines was developed by single seed descent method （SSD） based on a parent com- bination of small-grain indica cultivar Kasalath and large-grain japanica cultivar TD70 with significant differences in plant type traits, to construct the molecular genetic linkage map. Totally 838 SSR （Simple Sequence Repeat） markers were used for polymorphism screening between parents, 302 SSR markers with polymorphism were detected, with a frequency of 36.04%; 141 SSR markers with clear amplified bands and uniform distribution in the genome were finally used for genotype analysis of the RIL population. According to the experimental results, the frequency of male and female genotype in this RIL population was respectively 53% and 47%, suggesting good balance in population structure. A molecular genetic linkage map of rice was constructed by 141 markers based on a RIL population of 240 lines, with a total genetic distance of about 1 832.47 cM covering all 12 chromosomes, an average genetic distance between markers of 12.70 cM and a range of genetic distance be- tween markers of 0.43-36.11 cM, which is consistent with basic requirements of quantitative trait locus （QTL） mapping. Except for few markers on chromosomes 1 and 8, the order and location of markers is similar to the published sequences of Nipponbare. QTL analysis for the tiller angle was conducted with this RIL population of 240 lines, and results showed that three QTLs controlling tiller angle were detected on chromosome 8, 9 and 11, which were named qTA8, qTA9 and qTA11, with a contribution rate of 4.10%, 26.08% and 4.35%, respectively. To be specific, qTA9 contained Tiller Angle Controlling （TAC1） gene. The construction of this molecular genetic linkage map laid the foundation for genetic analysis and QTL mapping of various traits in the progeny of indica and japonica.展开更多
Objectives To identify the loci involved in nonsyndromic cleft lip with or without cleft palate （NSCL/P） in Northern Chinese people in Shenyang by using genomewide and interaction linkage scan.Methods Two multiplex ...Objectives To identify the loci involved in nonsyndromic cleft lip with or without cleft palate （NSCL/P） in Northern Chinese people in Shenyang by using genomewide and interaction linkage scan.Methods Two multiplex families in Shenyang from North China were ascertained through probands with NSCL/P.Blood of every member was drawn for DNA extraction and analysis.Genotypes were available for 382 autosomal short tandem repeat （STR） markers from the ABI Prism Linkage Mapping Set version 2.5.Linkage between markers and NSCL/P was assessed by 2-point parametric LOD scores,multipoint heterogeneity parametric LOD scores （HLODs）,and multipoint nonparametric linkage score （NPL）.Results The initial scan suggested linkage on Chromosomes 1,2,and 15.In subsequent fine mapping,1q32-q42 showed a maximum multipoint LOD score of 1.9（empirical P=0.013） and an NPL score of 2.35 （empirical P=0.053）.For 2p24-p25,the multipoint NPL increased to 2.94 （empirical P=0.007）.2-locus interaction analysis obtained a maximum NPL score of 3.73 （P=0.00078） and a maximum LOD score of 3 for Chromosome 1 （at 221 cM） and Chromosome 2 （at 29 cM）.Conclusion Both parametric and nonparametric linkage scores greatly increased over the initial linkage scores on 1q32-q42,suggesting a susceptibility locus in this region.Nonparametric linkage gave a strong evidence for a candidate region on chromosome 2p24-p25.The superiority of 2-locus linkage scores compared to single-locus scores gave additional evidence for linkage on 1q32-q42 and 2p24-p25,and suggested that certain genes in the two regions may contribute to NCSL/P risks with interaction.展开更多
基金funding support from the National Science and Technology Council(NSTC),under Grant No.114-2410-H-011-026-MY3.
文摘Small datasets are often challenging due to their limited sample size.This research introduces a novel solution to these problems:average linkage virtual sample generation(ALVSG).ALVSG leverages the underlying data structure to create virtual samples,which can be used to augment the original dataset.The ALVSG process consists of two steps.First,an average-linkage clustering technique is applied to the dataset to create a dendrogram.The dendrogram represents the hierarchical structure of the dataset,with each merging operation regarded as a linkage.Next,the linkages are combined into an average-based dataset,which serves as a new representation of the dataset.The second step in the ALVSG process involves generating virtual samples using the average-based dataset.The research project generates a set of 100 virtual samples by uniformly distributing them within the provided boundary.These virtual samples are then added to the original dataset,creating a more extensive dataset with improved generalization performance.The efficacy of the ALVSG approach is validated through resampling experiments and t-tests conducted on two small real-world datasets.The experiments are conducted on three forecasting models:the support vector machine for regression(SVR),the deep learning model(DL),and XGBoost.The results show that the ALVSG approach outperforms the baseline methods in terms of mean square error(MSE),root mean square error(RMSE),and mean absolute error(MAE).
文摘Against the backdrop of continuous social development and growing public health demands,the efficiency and scientific nature of the emergency care system are of paramount importance.This paper focuses on researching the construction of an emergency care system based on the concept of“linkage”,delving into its theoretical foundations,exploring innovative construction models,and analyzing practical cases.The study indicates that an emergency care system under the“linkage”concept can effectively integrate resources and enhance efficiency,providing new insights for improving the construction of the emergency care system.It aims to promote the development of the emergency care system towards a more scientific,efficient,and collaborative direction.
基金supported by the Biological Breeding-Major Projects in National Science and Technology(No.2023ZD0404405)the Earmarked Fund for China Agriculture Research System(No.CARS-pig-35)+2 种基金the National Natural Science Foundation of China(No.3227284,32302708)the 2115 Talent Development Program of China Agricultural University,the Chinese Universities Scientific Fund(No.2023TC196)the Seed Industry Revitalization Action Project of Guangdong Province(No.2024-XPY-06-001)。
文摘Background Multibreed genomic prediction(MBGP)is crucial for improving prediction accuracy for breeds with small populations,for which limited data are often available.Recent studies have demonstrated that partitioning the genome into nonoverlapping blocks to model heterogeneous genetic(co)variance in multitrait models can achieve higher joint prediction accuracy.However,the block partitioning method,a key factor influencing model performance,has not been extensively explored.Results We introduce mbBayesABLD,a novel Bayesian MBGP model that partitions each chromosome into nonoverlapping blocks on the basis of linkage disequilibrium(LD)patterns.In this model,marker effects within each block are assumed to follow normal distributions with block-specific parameters.We employ simulated data as well as empirical datasets from pigs and beans to assess genomic prediction accuracy across different models using cross-validation.The results demonstrate that mbBayesABLD significantly outperforms conventional MBGP models,such as GBLUP and BayesR.For the meat marbling score trait in pigs,compared with GBLUP,which does not account for heterogeneous genetic(co)variance,mbBayesABLD improves the prediction accuracy for the small-population breed Landrace by 15.6%.Furthermore,our findings indicate that a moderate level of similarity in LD patterns between breeds(with an average correlation of 0.6)is sufficient to improve the prediction accuracy of the target breed.Conclusions This study presents a novel LD block-based approach for multibreed genomic prediction.Our work provides a practical tool for livestock breeding programs and offers new insights into leveraging genetic diversity across breeds for improved genomic prediction.
基金Under the auspices of National Natural Science Foundation of China(No.42371181)。
文摘Promoting the deep integration of advanced manufacturing and producer services is not only a key path for metropolitan to climb the global value chain,but also an important new driving force for optimizing urban spatial structure.Based on the two dimen-sions of industrial linkage and spatial co-agglomeration from the perspective of industrial integration,taking Beijing,China as a case,using industrial input-output data,micro-enterprise spatial data,and methods such as industrial linkage model,standard deviation el-lipse,and spatial similarity coefficient,this study explores the coupling relationship between industrial linkage and spatial co-agglomer-ation of advanced manufacturing and producer services from 2007 to 2020.The results demonstrate that:1)the industrial linkage between advanced manufacturing and producer services in Beijing has an upward trend,in which the business services have the most significant improvement,in which the business services have the most improvement while the technology services have the most de-crease,but in general,the industrial linkage between the two industries shows a low level.2)Producer services were more spatially clustered than advanced manufacturing,and the degree of spatial co-agglomeration of the two also showed an increasing trend,with strong regional and industrial heterogeneity characteristics,among which the pairing industry involving commerce services and techno-logy services had a more obvious improvement.3)The coupling analysis found that the spatial dependence between advanced manufac-turing and producer services decreased,showing a weak positive correlation and a decreasing trend between industrial linkage and spa-tial co-agglomeration;the heterogeneity among subdivision pairs is prominent,and the strong correlation between industries does not mean that there is an inevitable spatial co-agglomeration.4)Based on the differences in coupling relationships and influence mechan-isms,four coupling modes are identified:H-H type,H-L type,L-L type,and L-H type.Finally,this study proposes three policy implica-tions for the integration of advanced manufacturing and producer services in metropolitan areas.
文摘Objective:To investigate the effects of performance-linked early rehabilitation management model on the recovery of neurological function,motor function and activities of daily living in patients after intracerebral hemorrhage surgery.Methods:A randomized controlled trial design was adopted.A total of 120 patients after cerebral hemorrhage surgery admitted to our hospital from June 2023 to June 2024 were selected as the research subjects and were randomly divided into the experimental group and the control group,with 60 cases in each group according to the random number table method.The control group received routine postoperative care and rehabilitation guidance in neurosurgery,while the experimental group implemented an efficacy linkage early rehabilitation management model on the basis of routine care.Before the intervention,4 weeks after the intervention,and 12 weeks after the intervention,the National Institutes of Health Stroke Scale(NIHSS),the Fugl-Meyer Assessment of Motor Function(FMA),and the Barthel Index(BI)were used to assess the degree of neurological deficits,motor function,and activities of daily living in patients,respectively.Results:After 4 weeks and 12 weeks of intervention,the NIHSS scores of the experimental group[(8.5±2.1)points,(5.2±1.8)points]were significantly lower than those of the control group[(10.8±2.4)points,(7.9±2.2)points](P<0.01);FMA scores[(58.4±10.2)points,(78.6±12.4)points]and BI scores[(55.3±11.5)points,(72.8±13.2)points]were significantly higher than those of the control group[(45.2±9.8)points,(62.3±11.7)points;(42.1±10.6)points,(58.4±12.5)points](P<0.01).Conclusion:The performance-linked early rehabilitation management model can effectively promote the recovery of neurological and motor functions in patients after cerebral hemorrhage surgery and significantly improve their ability to take care of themselves in daily living.It is an efficient rehabilitation management strategy.
基金supported by the Director General,Indian Council of Agricultural Research(ICAR),New Delhithe Director,ICAR-National Rice Research Institute,Cuttack.
文摘Consecutive stresses,such as initial submergence during germination followed by water deficit during the seedling stage,pose significant challenges to direct-seeded rice cultivation.By Linkage disequilibrium analysis,Sub1 and Dro1(Δbp:10 Mb),as well as Sub1 and TPP7(Δbp:6 Mb)were identified to exhibit long-range linkage disequilibrium(LRLD).Meta-QTL analysis further revealed that Sub1 and TPP7 co-segregated for tolerance to submergence at the germination and seedling stages.Based on this,we hypothesized that LRLD might influence plant responses to consecutive stresses.To test this hypothesis,we developed a structured recombinant inbred line population from a cross between Bhalum 2 and Nagina 22,with alleles(Sub1 and TPP7)in linkage equilibrium.Mendelian randomization analysis validated that the parental alleles,rather than the recombinant alleles of Sub1 and TPP7,significantly influenced 13 out of 41 traits under consecutive stress conditions.Additionally,16 minor additive effect QTLs were detected between the genomic regions,spanning Sub1 and TPP7 for various traits.A single allele difference between these genomic regions enhanced crown root number,root dry weight,and specific root area by 11.45%,15.69%,and 33.15%,respectively,under flooded germination conditions.Candidate gene analysis identified WAK79 and MRLK59 as regulators of stress responses during flooded germination,recovery,and subsequent water deficit conditions.These findings highlight the critical role of parental allele combinations and genomic regions between Sub1 and TPP7 in regulating the stress responses under consecutive stresses.Favourable haplotypes derived from these alleles can be utilized to improve stress resilience in direct-seeded rice.
基金“Research on Improving Clinical Thinking Practice of Higher Vocational Nursing Students in Internal Medicine Nursing Through Digital Narrative Education from the Perspective of New Quality Productive Forces”(Project No.:Y202507)。
文摘Objective:To address the problems of disconnection between teaching and practice,weak clinical thinking,and poor post adaptability of students in traditional internal medicine nursing teaching,explore the“dual-teacher linkage”collaborative teaching path,and improve teaching quality and students’post adaptability.Methods:Two classes of nursing majors in Grade 2023 of our college were selected as research objects.The experimental group(100 students)adopted the“dual-teacher linkage”model(on-campus teachers+clinical experts)to build a“pre-class co-research–in-class coguidance–post-class co-evaluation”system;the control group(98 students)adopted traditional teaching.Comparisons were made after one semester of practice.Results:The average score of the comprehensive assessment in the experimental group was 85.6 points(76.3 points in the control group,p<0.05).The clinical decision-making ability,program improvement ability,and post competence evaluation of the experimental group were all superior to those of the control group,and 93.3%of students were satisfied with this model.Conclusion:The“dual-teacher linkage”model can organically unify knowledge transmission and ability training,and is an effective and innovative practice to deepen the reform of internal medicine nursing teaching and cultivate high-quality skilled nursing talents.
基金financial support provided by the National Natural Science Foundation of Yunnan Province(202301AS070040)Major Science and Technology Projects of Yunnan Province(202302AB080019-3)。
文摘Covalent organic frameworks(COFs)play a crucial role in metal-free electrocatalysts for promoting oxygen reduction reaction(ORR)due to their adjustable skeleton structure and catalytic activity.While it is true that numerous studies have explored COFs for ORR,the critical gap by providing a systematic framework for ligand-driven electronic state manipulation is essential for designing highly active COF-based ORR catalysts.Herein,a series of COF-based metal-free materials have been conceived and synthesized by linkage-engineered strategy with dicarboxaldehyde(BPC),bipyridine-dicarbaldehyde(BPA)and benzodithiophene(BDA)as electronic linkages.Consequently,by incorporating different linkages into COFs,the surface area,electronic state,hydrophobic properties and affinities towards intermediates are optimized.Notably,the benzodithiophene-linked COF(denoted as BDA-COF)has greater catalytic ability with a half-wave potential of 0.74 V vs.RHE and an onset potential of 0.86 V vs.RHE than dicarboxaldehyde(denoted as BPC-COF)and dicarbaldehyde(denoted as BPA-COF).Relevant characterizations,in situ techniques and theoretical calculations confirm that thiophene-S-based COF promotes the electronic migration and enhances the interaction with the intermediate.The result provides insight into for illustration of a high-performance COF-based electrocatalyst via a linkage-engineered approach.
文摘Artificial photosynthesis of hydrogen peroxide(H_(2)O_(2))using covalent organic frameworks(COFs)as photocatalysts holds promise for future applications.However,the influence of linkage chemistry on the photoelectrochemical properties and photocatalytic performance of COFs remains a significant challenge.Herein,we designed and synthesized a model system with different linkages,including imine-,amine-,azo-linked COFs,then investigated their photocatalytic activity of overall H_(2)O_(2)production.The photocatalytic results revealed varying activities for H_(2)O_(2)synthesis among these COFs,with the azo-linked TTA-Azo-COF(COF synthesized by 4,4’,4’’-(1,3,5-triazine-2,4,6-triyl)-trianiline and terephthalaldehyde)demonstrating the highest overall H2O2 photosynthesis activity of 2516μmol g^(–1)h^(–1)in an O2 atmosphere without any sacrificial agents,which is 6.72 and 2.85 times higher than that of imine-linked TTA-COF and amine-linked TTA-COF-AR,respectively.Furthermore,TTA-Azo-COF maintained a high photosynthesis H2O2 activity of 2116μmol g^(–1)h^(–1)under an air atmosphere,outperforming most COF-based photocatalytic systems under similar reaction conditions.Further characterizations and density functional theory calculations reveal these various linkages in different COFs result in distinct visible-light absorption,charge transfer capacities and formation energy barriers of key intermediates.This work revealed the significant impact of linkages on COFs and provided comprehensive guidance for the rational design of COFs with tailored linkages to fulfill specific requirements for future applications.
基金supported by the National Natural Science Foundation of China(22305238)the Anhui Provincial Natural Science Foundation(2308085MB35)。
文摘The utilization of covalent organic frameworks(COFs)holds great potential for achieving tailorable tuning of catalytic performance through bottom-up modulation of the reticular structure.In this work,we show that a single-point structural alteration in the linkage within a nickel phthalocyanine(NiPc)-based series effectively modulates the catalytic performance of the COFs in electrochemical CO_(2)reduction reaction(CO_(2)RR).A Ni Pc-based COF series with three members which possess the same Ni Pc unit but different linkages,including piperazine,dioxin,and dithiine,have been constructed by nucleophilic aromatic substitution reaction between octafluorophthalocyanine nickel and tetrasubstituted benzene linkers with different bridging groups.Among these COFs,the dioxin-linked COF showed the best activity of CO_(2)RR with a current density of CO(j_(CO))=-27.99 m A cm^(-2)at-1.0 V(versus reversible hydrogen electrode,RHE),while the COF with piperazine linkage demonstrated an excellent selectivity of Faradaic efficiency for CO(FECO)up to 90.7%at a pretty low overpotential of 0.39 V.In addition,both a high FECO value close to 100%and a reasonable jCO of-8.20 m A cm^(-2)at the potential of-0.8 V(versus RHE)were obtained by the piperazine-linked COF,making it one of the most competitive candidates among COF-based materials.Mechanistic studies exhibited that single-point structural alteration could tailor the electron density in Ni sites and alter the interaction between the active sites and the key intermediates adsorbed and desorbed,thereby tuning the electrochemical performance during CO_(2)RR process.
文摘The ASEAN-China(Beijing Municipal Administrative Centre)Trade and Investment Promotion Conference serves as a new platform for fostering collaboration in trade,investment,and sustainable growth.The event,held in Tongzhou District-the municipal administrative center of Beijing-highlights shared aspirations for building an environmentally friendly and innovation-driven regional economy.
基金Project(71073177)supported by the National Natural Science Foundation of ChinaProject(12JJ4077)supported by the Natural Science Foundation of Hunan Province of ChinaProject(2012zzts002)supported by the Fundamental Research Funds of Central South University,China
文摘Using VAR-DCC-GARCH model,the literature on commodity price was extended by exploring the co-movement between Chinese nonferrous metal prices and global nonferrous metal prices represented by the nonferrous metal prices from London Metal Exchange(LME).The results show that LME nonferrous metals prices still have a greater impact on Chinese nonferrous metals prices.However,the impact of Chinese nonferrous metals prices on LME nonferrous metals prices is still weak except for lead price.The co-movement of nonferrous metal prices between LME and China presents hysteretic nature,and it lasts for 7-8trading days.Furthermore,the co-movement between LME nonferrous metals prices and Chinese nonferrous metals prices has the characteristics of time-varying,and the correlation of lead prices between LME and China is the more stable than all other nonferrous metals prices.
文摘In this study, we reported the repaid construction of a molecular marker linkage map of rice (Oryza sativa L.). An F-2 population from the cross between Annong S-1 and Nanjing 11 was used to construct a genetic linkage map of rice. Total of 142 newly screened AFLP markers and 30 anchor markers (25 SSR markers and 5 RFLP markers) were mapped on the 12 chromosomes covering 1537.4 cM of rice genome. The average interval between these markers was 9.0 cM. The total work which usually was finished in more than one year was finished within only 3 months by one person. This is the first plant AFLP map developed in China. A new thermosensitive genic male sterile gene in rice, tms5, was Egged and mapped onto chromosome 2 during the development of the linkage map.
文摘Prader-Willi Syndrome （PWS） is a genetic disorder that is difficult to detect, particularly at an early age. PWS is caused by disruption of normal, epigenetically controlled gene function in the chromosome 15q11-q13 region. Clinical symptoms are difficult to diagnose in infants and only become clearer at later ages as the patients develop hyperphagia and morbid obesity. Molecular genetic tests are able to definitively diagnose PWS and allow early diagnosis of the syndrome. High resolution cytogenetic testing, methylation-specific PCR （MS-PCR）, and linkage analysis are routinely used to diagnose PWS. To establish a linkage analysis method for Chinese patients, this study identified a useful set of STR markers in the typical PWS deletion and adjacent area, for linkage analysis in two Chinese families with PWS offspring. Using this method, the authors confn＇rned that one patient had a paternal deletion in chromosome 15q 11-q 13 and the other patient had maternal uniparental heterodisomy of chromosome 15. MS -PCR and high resolution chromosome G-banding also confirmed this diagnosis. This linkage analysis method can detect both deletion and uniparental disomy, thus providing valuable information for genetic counseling and the opportunity to analyze the relationship between the genotype and phenotype of PWS.
基金Supported by Scientific Research Grant of the Education Department of Guangxi Zhuang Autonomous Region(200807MS065)Elite Support Fund for Universities in Guangxi Zhuang Autonomous Region~~
文摘[Objective]The aim was to discuss elimination mechanism and equilibrium model about complete linkage gene of panmixis based on fitness.[Method]A mathematical model that gsgenerational evalution of frequency of panmixis two pairs of complete linkage gene under difference fitness was built by using difference equations to study elimination mechanism and genetic equilibrium state of panncticcoinplete linkage gene.[Result]The panmixis is different from Hardy-Weinberg Equilibrium which reached the equilibrium just by one generation.A method that by using linear equation to calculate the frequency of four gametes and the fitnessed of panmixis was established after detormining is the fitness of 10 genotypes the necessary and sufficient condition of the panmixis to reach balance.[Conclusion]The method is valuable for reference on linkage disequilibrium and hitchhiking effect study.
基金Supported by Fund for Agricultural Science and Technology Innovation in Jiangsu Province(CX[12]1003)Science Technology Pillar Program in Jiangsu Province(BK2013303)Supper Rice Breeding and Demonstration Program of the Ministry ofAgriculture~~
文摘In this study, an RIL （recombinant inbred line） population containing 240 lines was developed by single seed descent method （SSD） based on a parent com- bination of small-grain indica cultivar Kasalath and large-grain japanica cultivar TD70 with significant differences in plant type traits, to construct the molecular genetic linkage map. Totally 838 SSR （Simple Sequence Repeat） markers were used for polymorphism screening between parents, 302 SSR markers with polymorphism were detected, with a frequency of 36.04%; 141 SSR markers with clear amplified bands and uniform distribution in the genome were finally used for genotype analysis of the RIL population. According to the experimental results, the frequency of male and female genotype in this RIL population was respectively 53% and 47%, suggesting good balance in population structure. A molecular genetic linkage map of rice was constructed by 141 markers based on a RIL population of 240 lines, with a total genetic distance of about 1 832.47 cM covering all 12 chromosomes, an average genetic distance between markers of 12.70 cM and a range of genetic distance be- tween markers of 0.43-36.11 cM, which is consistent with basic requirements of quantitative trait locus （QTL） mapping. Except for few markers on chromosomes 1 and 8, the order and location of markers is similar to the published sequences of Nipponbare. QTL analysis for the tiller angle was conducted with this RIL population of 240 lines, and results showed that three QTLs controlling tiller angle were detected on chromosome 8, 9 and 11, which were named qTA8, qTA9 and qTA11, with a contribution rate of 4.10%, 26.08% and 4.35%, respectively. To be specific, qTA9 contained Tiller Angle Controlling （TAC1） gene. The construction of this molecular genetic linkage map laid the foundation for genetic analysis and QTL mapping of various traits in the progeny of indica and japonica.
基金supported by National Natural Science Foundation of China (the research to identify susceptibility genes of nonsyndromic cleft lip and/or palates, 30600676)Program for New Century Excellent Talents of the Ministry of Education of China (NCET-07-0034)
文摘Objectives To identify the loci involved in nonsyndromic cleft lip with or without cleft palate （NSCL/P） in Northern Chinese people in Shenyang by using genomewide and interaction linkage scan.Methods Two multiplex families in Shenyang from North China were ascertained through probands with NSCL/P.Blood of every member was drawn for DNA extraction and analysis.Genotypes were available for 382 autosomal short tandem repeat （STR） markers from the ABI Prism Linkage Mapping Set version 2.5.Linkage between markers and NSCL/P was assessed by 2-point parametric LOD scores,multipoint heterogeneity parametric LOD scores （HLODs）,and multipoint nonparametric linkage score （NPL）.Results The initial scan suggested linkage on Chromosomes 1,2,and 15.In subsequent fine mapping,1q32-q42 showed a maximum multipoint LOD score of 1.9（empirical P=0.013） and an NPL score of 2.35 （empirical P=0.053）.For 2p24-p25,the multipoint NPL increased to 2.94 （empirical P=0.007）.2-locus interaction analysis obtained a maximum NPL score of 3.73 （P=0.00078） and a maximum LOD score of 3 for Chromosome 1 （at 221 cM） and Chromosome 2 （at 29 cM）.Conclusion Both parametric and nonparametric linkage scores greatly increased over the initial linkage scores on 1q32-q42,suggesting a susceptibility locus in this region.Nonparametric linkage gave a strong evidence for a candidate region on chromosome 2p24-p25.The superiority of 2-locus linkage scores compared to single-locus scores gave additional evidence for linkage on 1q32-q42 and 2p24-p25,and suggested that certain genes in the two regions may contribute to NCSL/P risks with interaction.
