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On-line SPE/LC-LIT-MS法快速测定全血和肝组织中的利多卡因及其代谢物
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作者 卢敏萍 莫旖 +4 位作者 何日梅 黄克建 刘晓锋 杨宁 乔文涛 《化学研究与应用》 北大核心 2026年第1期228-233,共6页
基于在线固相萃取(on-line SPE)前处理技术,建立了一种快速测定全血和肝组织样品中利多卡因(LDC)及其代谢物单乙基甘氨酰二甲基苯胺(MEGX)、甘氨酰二甲基苯胺(GX)的液相色谱-线性离子阱质谱(LC-LIT-MS)分析方法。用乙腈沉淀样品中的蛋白... 基于在线固相萃取(on-line SPE)前处理技术,建立了一种快速测定全血和肝组织样品中利多卡因(LDC)及其代谢物单乙基甘氨酰二甲基苯胺(MEGX)、甘氨酰二甲基苯胺(GX)的液相色谱-线性离子阱质谱(LC-LIT-MS)分析方法。用乙腈沉淀样品中的蛋白,再经稀释、离心、过滤后即可进样。采用Waters Oasis®HLB在线SPE柱对样品进行快速富集纯化,结束后,通过阀切换将样品转移至Syncronis C8分析柱进行梯度洗脱,流动相为均含0.1%甲酸(V/V)的0.01 mol·L^(-1)乙酸铵溶液(A)和甲醇(B),最后使用电喷雾电离正离子源(ESI^(+)),在多反应监测(MRM)模式下进行测定,对利多卡因及其代谢物实现定性筛查与定量分析。在考察的浓度范围内,三种分析物均获得了良好的线性关系,相关系数为0.998 3~0.999 6,全血样品的检出限为(0.1~2.0)ng·mL^(−1),肝组织样品的检出限为(0.1~2.0)ng·g^(-1),3个添加水平的回收率为94.0~108.6%,相对标准偏差为0.1~5.8%(n=6)。该方法避免了繁琐的前处理过程,简单灵敏,结果准确可靠,适用于全血和肝组织样品中利多卡因及其代谢物的快速测定。 展开更多
关键词 在线固相萃取 LC-LIT-MS法 利多卡因 代谢物 全血 肝组织
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Emphasizing Theoretical Innovation in the Renovation ofExisting Urban Rail Transit Lines
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作者 DING Shukui 《城市轨道交通研究》 北大核心 2026年第1期167-168,共2页
Over the next 20 years,China's urban rail transit(hereinafter referred to as'urban rail')will face large-scalerenovation of existing line facilities and equipment,with more than 1000 km of renovated lines ... Over the next 20 years,China's urban rail transit(hereinafter referred to as'urban rail')will face large-scalerenovation of existing line facilities and equipment,with more than 1000 km of renovated lines to be added eachyear.In 2024,the China Association of Metros issued the Guiding Opinions on the Renovation of Existing UrbanRail Transit Lines in China,providing guiding opinions on norms,standards,and implementation approaches forthe renovation of existing lines in the coming period.In the practical work of renovating existing urban rail lines,it is necessary to continuously explore and refine relevant theoretical methods in line with industry developmenttrends and urban development requirements.The following are the author's recent reflections on theoreticalinnovation in this field. 展开更多
关键词 urban rail transit industry development trends existing lines renovation existing lines urban rail transit hereinafter RENOVATION urbanrail transit lines theoretical innovation
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基于转座元件分析揭示绵羊基因组中LINE转座子元件的特性和进化
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作者 梁千千 董华娇 +4 位作者 李甜甜 张洁 杨敏 万鹏程 韩吉龙 《内蒙古农业大学学报(自然科学版)》 北大核心 2025年第6期1-8,共8页
本研究采用不同的3种方法对绵羊基因组进行注释,筛选出适合绵羊转座元件注释的方法,并进行转座元件的演化历史分析。结果表明,采用从头预测(de novo prediction)和同源预测(homology?based prediction)相结合的策略对绵羊基因组进行注... 本研究采用不同的3种方法对绵羊基因组进行注释,筛选出适合绵羊转座元件注释的方法,并进行转座元件的演化历史分析。结果表明,采用从头预测(de novo prediction)和同源预测(homology?based prediction)相结合的策略对绵羊基因组进行注释效果最佳,鉴定出绵羊基因组中转座元件占比为47.34%,其中长散布核元件(LINE)占比最高(35.34%),其余依次为长末端重复序列(LTR,5.43%)、短散布核元件(SINE,3.66%)、DNA转座元件(2.44%)和未知家族(0.56%)。LINE中的牛B型逆转录转座元件(RTE?BovB)占比达16.47%。转座元件在绵羊基因组中广泛分布,但在X染色体上富集显著,占比高达57.22%。研究通过Kimura双参数模型分析转座元件的演化历史,发现绵羊基因组经历了两次转座“爆发”事件:第1次以LINE转座元件为主,第2次则同时涉及LINE和SINE转座元件的扩增,且具有时序性。综上,LINE转座元件的持续活跃是绵羊基因组扩增的主要驱动力,且其演化历史与反刍动物的基因组进化密切相关。 展开更多
关键词 绵羊 基因组 长散布核元件(line) 基因组扩增 适应性进化
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A 2.69 ppm/℃ bandgap reference with 42 ppm/V line sensitivity for battery management system 被引量:1
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作者 Jing Wang Feixiang Zhang +2 位作者 Zhiyuan He Hui Zhang Lin Cheng 《Journal of Semiconductors》 2025年第6期31-42,共12页
This paper introduces a high-precision bandgap reference(BGR)designed for battery management systems(BMS),fea-turing an ultra-low temperature coefficient(TC)and line sensitivity(LS).The BGR employs a current-mode sche... This paper introduces a high-precision bandgap reference(BGR)designed for battery management systems(BMS),fea-turing an ultra-low temperature coefficient(TC)and line sensitivity(LS).The BGR employs a current-mode scheme with chopped op-amps and internal clock generators to eliminate op-amp offset.A low dropout regulator(LDO)and a pre-regula-tor enhance output driving and LS,respectively.Curvature compensation enhances the TC by addressing higher-order nonlinear-ity.These approaches,effective near room temperature,employs trimming at both 20 and 60°C.When combined with fixed cur-vature correction currents,it achieves an ultra-low TC for each chip.Implemented in a CMOS 180 nm process,the BGR occu-pies 0.548 mm²and operates at 2.5 V with 84μA current draw from a 5 V supply.An average TC of 2.69 ppm/℃ with two-point trimming and 0.81 ppm/℃ with multi-point trimming are achieved over the temperature range of-40 to 125℃.It accommo-dates a load current of 1 mA and an LS of 42 ppm/V,making it suitable for precise BMS applications. 展开更多
关键词 bandgap reference high precision low temperature coefficient small line sensitivity battery management system BMS
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Residual rock deformation of lined caverns for underground energy storage after air deflation considering stress path 被引量:1
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作者 Chen Xu Caichu Xia +3 位作者 Gecheng Zhang Sheng Wang Hui Lu Yingjun Xu 《Journal of Rock Mechanics and Geotechnical Engineering》 2025年第7期4160-4178,共19页
This study investigates the mechanical response of an underground cavern subjected to cyclic high gas pressure,aiming to establish a theoretical foundation for the design of lined rock caverns(LRCs)for energy storage ... This study investigates the mechanical response of an underground cavern subjected to cyclic high gas pressure,aiming to establish a theoretical foundation for the design of lined rock caverns(LRCs)for energy storage with high internal pressure,e.g.compressed air energy storage(CAES)underground caverns or hydrogen storage caverns.Initially,the stress paths of the surrounding rock during the excavation,pressurization,and depressurization processes are delineated.Analytical expressions for the stress and deformation of the surrounding rock are derived based on the MohreCoulomb criterion.These expressions are then employed to evaluate the displacement of cavern walls under varying qualities of surrounding rock,the contact pressure between the steel lining and the surrounding rock subject to different gas storage pressures,the load-bearing ratio of the surrounding rock,and the impact of lining thickness on the critical gas pressure.Furthermore,the deformation paths of the surrounding rock are evaluated,along with the effects of tunnel depth and diameter on residual deformation of the surrounding rock,and the critical minimum gas pressure at which the surrounding rock and the lining do not detach.The results indicate that residual deformation of the surrounding rock occurs after depressurization under higher internal pressure for higher-quality rock masses,leading to detachment between the surrounding rock and the steel lining.The findings indicate that thicker linings correspond to higher critical minimum gas pressures.However,for lower-quality surrounding rock,thicker linings correspond to lower critical minimum gas pressures.These findings will provide invaluable insights for the design of LRCs for underground energy storage caverns. 展开更多
关键词 Underground energy storage lined rock cavern(LRC) Cyclic high pressure Mechanical response Stress path
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GFRF R-CNN:Object Detection Algorithm for Transmission Lines
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作者 Xunguang Yan Wenrui Wang +3 位作者 Fanglin Lu Hongyong Fan Bo Wu Jianfeng Yu 《Computers, Materials & Continua》 SCIE EI 2025年第1期1439-1458,共20页
To maintain the reliability of power systems,routine inspections using drones equipped with advanced object detection algorithms are essential for preempting power-related issues.The increasing resolution of drone-cap... To maintain the reliability of power systems,routine inspections using drones equipped with advanced object detection algorithms are essential for preempting power-related issues.The increasing resolution of drone-captured images has posed a challenge for traditional target detection methods,especially in identifying small objects in high-resolution images.This study presents an enhanced object detection algorithm based on the Faster Regionbased Convolutional Neural Network(Faster R-CNN)framework,specifically tailored for detecting small-scale electrical components like insulators,shock hammers,and screws in transmission line.The algorithm features an improved backbone network for Faster R-CNN,which significantly boosts the feature extraction network’s ability to detect fine details.The Region Proposal Network is optimized using a method of guided feature refinement(GFR),which achieves a balance between accuracy and speed.The incorporation of Generalized Intersection over Union(GIOU)and Region of Interest(ROI)Align further refines themodel’s accuracy.Experimental results demonstrate a notable improvement in mean Average Precision,reaching 89.3%,an 11.1%increase compared to the standard Faster R-CNN.This highlights the effectiveness of the proposed algorithm in identifying electrical components in high-resolution aerial images. 展开更多
关键词 Faster R-CNN transmission line object detection GIOU GFR
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Aerodynamics and countermeasures of train-tail swaying inside single-line tunnels 被引量:1
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作者 Yadong SONG Yanpeng ZOU +2 位作者 Yuan YAO Ting QIN Longjiang SHEN 《Journal of Zhejiang University-Science A(Applied Physics & Engineering)》 2025年第5期438-455,共18页
In recent years,train-tail swaying of 160 km/h electric multiple units(EMUs)inside single-line tunnels has been heavily researched,because the issue needs to be solved urgently.In this paper,a co-simulation model of v... In recent years,train-tail swaying of 160 km/h electric multiple units(EMUs)inside single-line tunnels has been heavily researched,because the issue needs to be solved urgently.In this paper,a co-simulation model of vortex-induced vibration(VIV)of the tail car body is established,and the aerodynamics of train-tail swaying is studied.The simulation results were confirmed through a field test of operating EMUs.Furthermore,the influence mechanism of train-tail swaying on the wake flow field is studied in detail through a wind-tunnel experiment and a simulation of a reduced-scaled train model.The results demonstrate that the aerodynamic force frequency(i.e.,vortex-induced frequency)of the train tail increases linearly with train speed.When the train runs at 130 km/h,with a small amplitude of train-tail swaying(within 10 mm),the vortex-induced frequency is 1.7 Hz,which primarily depends on the nose shape of the train tail.After the tail car body nose is extended,the vortex-induced frequency is decreased.As the swaying amplitude of the train tail increases(exceeding 25 mm),the separation point of the high-intensity vortex in the train wake shifts downstream to the nose tip,and the vortex-induced frequency shifts from 1.7 Hz to the nearby car body hunting(i.e.,the primary hunting)frequency of 1.3 Hz,which leads to the frequency-locking phenomenon of VIV,and the resonance intensifies train-tail swaying.For the motor vehicle of the train tail,optimization of the yaw damper to improve its primary hunting stability can effectively alleviate train-tail swaying inside single-line tunnels.Optimization of the tail car body nose shape reduces the amplitude of the vortex-induced force,thereby weakening the aerodynamic effect and solving the problem of train-tail swaying inside the single-line tunnels. 展开更多
关键词 train tail swaying wind tunnel experiment field test single line tunnels AERODYNAMICS co simulation electric multiple units emus inside vortex induced vibration
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基于自适应EDLines和鲁棒位姿优化的视觉SLAM算法
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作者 王浩森 张丽杰 《计算机仿真》 2025年第9期355-361,共7页
同时定位与地图构建(SLAM)广泛应用于无人驾驶、机器人等多个领域,为了提高ORB-SLAM3在光照不均和弱纹理场景中的定位精度和鲁棒性,提出了一种基于自适应EDLines和鲁棒位姿优化的视觉SLAM算法。首先,提出了一种自适应EDLines线段检测算... 同时定位与地图构建(SLAM)广泛应用于无人驾驶、机器人等多个领域,为了提高ORB-SLAM3在光照不均和弱纹理场景中的定位精度和鲁棒性,提出了一种基于自适应EDLines和鲁棒位姿优化的视觉SLAM算法。首先,提出了一种自适应EDLines线段检测算法,根据像素梯度的变化更新梯度阈值,并设计相似线段删除和分割线段合并策略,提高EDLines提取线特征的准确性和完整性。其次,提出一种与线段端点无关的线特征残差计算方法,避免后端优化过程中不同视角对位姿估计结果的影响。实验结果表明,提出的视觉SLAM算法比ORB-SLAM3具有更高的定位精度和鲁棒性。 展开更多
关键词 同时定位与地图构建 线特征 线特征提取 位姿优化
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Design of a wideband non-uniform microstrip line for complex impedance matching in automotive radar frequencies
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作者 ZHANG Shuang-Gen YU Tao +2 位作者 WANG Yu-Lan CHENG Zhi-Hua YAO Jian-Quan 《红外与毫米波学报》 北大核心 2025年第2期168-176,共9页
Microstrip transmission lines connecting to the millimeter wave radar chip and antenna significantly affect the radiation efficiency and bandwidth of the antenna.Here,a wideband non-uniform wavy microstrip line for co... Microstrip transmission lines connecting to the millimeter wave radar chip and antenna significantly affect the radiation efficiency and bandwidth of the antenna.Here,a wideband non-uniform wavy microstrip line for complex impedance in automotive radar frequency range is proposed.Unlike the gradient transmission line,the wavy structure is composed of periodically semi-circular segments.By adjusting the radius of the semi-circular,the surface current is varied and concentrated on the semi-circular segments,allowing a wider tunability range of the resonant frequency.The results reveal that the bandwidth of the loaded wavy transmission line antenna improves to 9.37 GHz,which is 5.81 GHz wider than that of the loaded gradient line.The gain and the half power beam width of the loaded antenna are about 14.69 dB and 9.58°,respectively.The proposed non-uniform microstrip line scheme may open up a route for realizing wideband millimeter-wave automotive radar applications. 展开更多
关键词 millimeter wave gradient line wavy transmission line broadband high gain array antenna
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A C_(6)-decomposition of theλ-fold Line Graph of K_(x,y)
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作者 XIN Yue YANG Weihua 《数学进展》 北大核心 2025年第6期1205-1222,共18页
In this paper,we prove that L(K_(x,y))(λ),theλ-fold line graph of the complete bipartite graph Ka,y,has a C_(6)-decomposition if and only if ry≥6,λxy(c+y-2)=0(mod 12)and(x+y)=0(mod 2),where x,y are nonnegative int... In this paper,we prove that L(K_(x,y))(λ),theλ-fold line graph of the complete bipartite graph Ka,y,has a C_(6)-decomposition if and only if ry≥6,λxy(c+y-2)=0(mod 12)and(x+y)=0(mod 2),where x,y are nonnegative integers and(x,y)≠(2,4)or(2,5). 展开更多
关键词 cycle decomposition line graph complete bipartite graph
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Impact of Single-Phase Automatic Recloser of Critical Transmission Line on the Stability of the Power Transmission Network
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作者 Chuulan Natsagdorj Ganbat Gantamir Erdenebileg Doljinsuren 《Journal of Power and Energy Engineering》 2025年第2期43-56,共14页
In order to increase the stability of the Mongolia power system, a single-phase automatic reclosing device (SPAR) was introduced on double-circuit power lines built with a size of 330 kV, operating on a voltage of 220... In order to increase the stability of the Mongolia power system, a single-phase automatic reclosing device (SPAR) was introduced on double-circuit power lines built with a size of 330 kV, operating on a voltage of 220 kV and a length of 250 km. These overhead power lines (L-213, L-214) connect the 220/110/35 kV “Songino” substation with the “Mandal” substation and form system networks. This paper presents the challenges encountered when implementing single-phase automatic reclosing (SPAR) devices and compares the changes in power system parameters before and after SPAR deployment for a long 220 kV line. Simulations and analyses were carried out using DIgSILENT PowerFactory software, focusing on rotor angle stability, and the overall impact on the power system during short-circuit faults. The evaluation also utilized measurement data from the Wide Area Monitoring System (WAMS) to compare system behavior pre- and post-implementation of SPAR. The findings reveal that SPAR significantly enhances system reliability and stability, effectively mitigating the risk of oscillations and stability loss triggered by short circuits. This improvement contributes to a more resilient power system, reducing the potential for disturbances caused by faults. 展开更多
关键词 Automatic Reclosing Single-Phase Automatic Reclosing Relay Protection Overhead Power lines System Stability Rotor Angle Stability
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Compact Ultra-Low Loss Optical True Delay Line on Thin Film Lithium Niobate
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作者 Yuan Ren Bo-Yang Nan +6 位作者 Rong-Bo Wu Yong Zheng Rui-Xue Liu Xue-Wen Wang Yun-Peng Song Min Wang Ya Cheng 《Chinese Physics Letters》 2025年第7期145-149,共5页
We report the fabrication of an 8-meter-long thin-flm lithium niobate optical true delay line using the photolithography-assisted chemomechanical etching technique,showing a low transmission loss of 0.036 dB/cm in the... We report the fabrication of an 8-meter-long thin-flm lithium niobate optical true delay line using the photolithography-assisted chemomechanical etching technique,showing a low transmission loss of 0.036 dB/cm in the conventional telecom band. 展开更多
关键词 conventional telecom band COMPACT transmission loss optical true delay line true delay line thin film ultra low loss lithium niobate
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China’s Sea Lines of Communication Security: A Perspective from a Holistic Approach to National Security
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作者 Wang Xu 《China International Studies》 2025年第4期31-68,共38页
Sea lines of communication(SLOCs)security has long been a strategic concern for major powers.Following the establishment of the People’s Republic of China,the country’s focus was on the traditional security aspects ... Sea lines of communication(SLOCs)security has long been a strategic concern for major powers.Following the establishment of the People’s Republic of China,the country’s focus was on the traditional security aspects of its SLOCs.Since the reform and opening-up era-and especially after the end of the Cold War-China has shifted its emphasis toward economic security.China’s SLOCs security bears on multiple dimensions of a holistic approach to national security,encompassing economic security,the safety of its citizens. 展开更多
关键词 holistic approach economic securitythe citizen safety sea lines communication slocs security national security sea lines communication security economic security
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Quick Author Guideline for OIL CROP SCIENCE
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《Oil Crop Science》 2025年第2期F0003-F0003,共1页
Manuscript Text and figures combined into a single file with page and line numbers up to3 MB in size in txt,doc,docx or tex files.Prepares your manuscript in the following order:Title page,Abstract,Introduction,Materi... Manuscript Text and figures combined into a single file with page and line numbers up to3 MB in size in txt,doc,docx or tex files.Prepares your manuscript in the following order:Title page,Abstract,Introduction,Materials and Methods,Results,Discussion,Acknowledgments,References,Tables,Figure Legends and Figures.Provide Cover letter and Supplementary Material(if necessary)at the same time. 展开更多
关键词 line oil crop text PAGE cover letter supplementary material SCIENCE MANUSCRIPT
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Establishment of a Leopard Coral Grouper(Plectropomus leopardus)Fin Cell Line(PLF):A Robust Tool for Investigating Viral Pathogenesis and Host Immunity
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作者 CHEN Huiquan XIAO Bingyan +2 位作者 CHEN Leshi YI Meisheng JIA Kuntong 《Journal of Ocean University of China》 2025年第6期1737-1746,共10页
Leopard coral grouper(Plectropomus leopardus)is a commercially important marine fish species.It is important to study how to prevent it from infecting with various viruses.In this study,we established and characterize... Leopard coral grouper(Plectropomus leopardus)is a commercially important marine fish species.It is important to study how to prevent it from infecting with various viruses.In this study,we established and characterized a new cell line derived from the fin tissue of leopard coral grouper(PLF).The PLF cells were cultured for more than 55 passages.Cytochrome B gene sequencing confirmed the origin of the PLF cells is P.leopardus.Immunostaining against cytokeratin indicated that the PLF cells predominantly consist of epithelial cells.The chromosome number of PLF was 48.The cells grew well in Dulbecco's modified Eagle's medium(DMEM)supplemented with 10%–20%fetal bovine serum(FBS)at temperature between 20–28℃,with the highest growth rate at28℃.Transfection with pEGFP-N3 plasmid showed the transfection efficiency was about 35%.Virus susceptibility tests revealed that PLF cells are susceptible to red-spotted grouper nervous necrosis virus(NNV)and viral hemorrhagic septicemia virus(VHSV),and viral proliferation was confirmed by qRT-PCR and western blot.The altered expressions of immune-related genes TBK1,IRF3,and Mx after NNV and VHSV infections suggested that PLF cells can mount an immune response to fish viruses.Thus the PLF cells can be employed for studying virus-host interactions and developing antiviral strategies. 展开更多
关键词 Plectropomus leopardus cell line RGNNV VHSV virus susceptibility
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A Study of Multiple Molecular Lines at the 3 mm Band toward Gas Infalling Sources
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作者 Yang Yang Xi Chen +3 位作者 Zhibo Jiang Zhiwei Chen Shuling Yu Jun Li 《Research in Astronomy and Astrophysics》 2025年第5期230-245,共16页
The study of multiple molecular spectral lines in gas infalling sources can provide the physical and chemica properties of these sources and help us estimate their evolutionary stages.We report line detections within ... The study of multiple molecular spectral lines in gas infalling sources can provide the physical and chemica properties of these sources and help us estimate their evolutionary stages.We report line detections within the 3 mm band using the FTS wide-sideband mode of the IRAM 30 m telescope toward 20 gas-infalling sources.Using XCLASS,we identify the emission lines of up to 22 molecular species(including a few isotopologues)and on hydrogen radio recombination line in these sources.H^(13)CO^(+),HCO^(+),HCN,HNC,c-C_(3)H_(2),and CCH lines are detected in 15 sources.We estimate the rotation temperatures and column densities of these molecular species using the LTE radiative transfer model,and compare the molecular abundances of these sources with those from nine high mass star-forming regions reported in previous studies and with those from the chemical model.Our results sugges that G012.79-0.20,G012.87-0.22 clump A and B,and G012.96-0.23 clump A may be in the high-mass protostella object stage,while sources with fewer detected species may be in the earlier evolutionary stage.Additionally,th CCH and c-C_(3)H_(2)column densities in our sources reveal a linear correlation,with a ratio of N(CCH)/N(c-C_(3)H_(2)=89.2±5.6),which is higher than the ratios reported in the literature.When considering only sources with lowe column densities,this ratio decreases to 29.0±6.1,consistent with those of diffuse clouds.Furthermore,comparison between the N(CCH)/N(c-C_(3)H_(2))ratio and the sources’physical parameters reveals a correlation,with sources exhibiting higher ratios tending to have higher kinetic temperatures and H2column densities. 展开更多
关键词 GALAXIES star formation-ISM clouds-ISM lines and bands-ISM MOLECULES
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HiFAST: An H I Data Calibration and Imaging Pipeline for FAST.Ⅲ.Standing Wave Removal
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作者 Chen Xu Jie Wang +13 位作者 Yingjie Jing Fujia Li Hengqian Gan Ziming Liu Tiantian Liang Qingze Chen Zerui Liu Zhipeng Hou Hao Hu Huijie Hu Shijie Huang Peng Jiang Chuan-Peng Zhang Yan Zhu 《Research in Astronomy and Astrophysics》 2025年第1期247-261,共15页
The standing waves existing in radio telescope data are primarily due to reflections among the instruments,which significantly impact the spectral quality of the Five-hundred-meter Aperture Spherical radio Telescope(F... The standing waves existing in radio telescope data are primarily due to reflections among the instruments,which significantly impact the spectral quality of the Five-hundred-meter Aperture Spherical radio Telescope(FAST).Eliminating these standing waves for FAST is challenging given the constant changes in their phases and amplitudes.Over a ten-second period,the phases shift by 18°while the amplitudes fluctuate by 6 mK.Thus,we developed the fast Fourier transform(FFT)filter method to eliminate these standing waves for every individual spectrum.The FFT filter can decrease the rms from 3.2 to 1.15 times the theoretical estimate.Compared to other methods such as sine fitting and running median,the FFT filter achieves a median rms of approximately 1.2 times the theoretical expectation and the smallest scatter at 12%.Additionally,the FFT filter method avoids the flux loss issue encountered with some other methods.The FFT is also efficient in detecting harmonic radio frequency interference(RFI).In the FAST data,we identified three distinct types of harmonic RFI,each with amplitudes exceeding 100 mK and intrinsic frequency periods of 8.1,0.5,and 0.37 MHz,respectively.The FFT filter,proven as the most effective method,is integrated into the H I data calibration and imaging pipeline for FAST(HiFAST,https://hifast.readthedocs.io). 展开更多
关键词 methods data analysis-techniques image processing-galaxies ISM-radio lines GALAXIES
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Retraction:Puerarin inhibits proliferation and induces apoptosis by upregulation of miR-16 in bladder cancer cell line T24
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作者 Oncology Research Editorial Office 《Oncology Research》 2025年第4期993-993,共1页
The published article titled“Puerarin inhibits proliferation and induces apoptosis by upregulation of miR-16 in bladder cancer cell line T24”has been retracted from Oncology Research,Vol.26,No.8,2018,pp.1227–1234.
关键词 PUERARIN bladder cancer bladder cancer cell line T miR APOPTOSIS PROLIFERATION
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Evaluation of transgenic cotton lines expressing an insecticidal fern protein against whitefly,Bemisia tabaci(Gennadius)
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作者 KUMAR Rishi NAGRARE V.S. +14 位作者 SHAH Vivek SINGH Satnam PANDHER Suneet SINGH Satpal VERMA S.K. PAUL Debashis RATHORE Pankaj SHUKLA Anoop Kumar SINGH Mithlesh Kumar SAURABH Sharad KUMAR Harish KAUR Rupinderjeet SINGH Pradhyumna Kumar WAGHMARE V.N. PRASAD Y.G. 《Journal of Cotton Research》 2025年第2期163-177,共15页
Background Transgenic research in crops involves using genetic engineering techniques to introduce specific genes of interest from other organisms,or even entirely new genes into plant genomes to create crops with des... Background Transgenic research in crops involves using genetic engineering techniques to introduce specific genes of interest from other organisms,or even entirely new genes into plant genomes to create crops with desirable traits that wouldn’t be possible through conventional breeding methods.Transgenic crops have been developed for various traits globally.Whitefly,Bemisia tabaci(Gennadius)is one of the major sucking pests of cotton that cause significant damage to the cotton production.To combat whitefly infestations,researchers have developed four transgenic cotton lines expressing the fern protein.And those transgenic lines need to be evaluated for their performance against the target pest—whitefly.The evaluation was designed as controlled trials in polyhouse or muslin cloth cages under open-choice and no-choice conditions by comparing four transgenic cotton lines(A,B,C,and D)with three control groups,including untransformed cotton plants with a same genetic background of the transgenic line,conventionally bred whitefly-resistant cotton,and whitefly-susceptible cotton.In order to study the generational effect,the evaluation also involved studies on whitefly development in laboratory,muslin cloth cage,and polyhouse conditions.Results Both open-choice and no-choice experiments had shown that all the four transgenic cotton lines(A,B,C,and D)expressing the fern protein reduced adult whitefly numbers significantly compared with the control lines,except for the no-choice conditions in 2021,where the transgenic line C was non-significant different from the resistant control line.Notably,the nymphal population on the resistant control line was relatively low and nonsignificant different from the transgenic line C in 2021;and the transgenic lines A and C in 2022 under open-choice conditions.Under no-choice condition,the nymphal counts in the resistant control line was non-significant different from transgenic lines C and D in 2021;and transgenic line D in 2022.All transgenic lines showed significant decrease in egg hatching in 2021 and nymphal development in 2022,except for the transgenic line C which had no significant different in the nymphal development comparing with non-transgenic control lines in 2022.Adult emergence rates in both years of evaluation showed significant decrease in transgenic lines A and B comparing with the control lines.Additionally,the results showed a significant reduction in cotton leaf curl disease and sooty mold development in all the four transgenic lines compared with susceptible control under open-choice conditions,indicating potential benefits of transgenic lines beyond direct effect on whitefly control.Furthermore,the research explored the generational effects of the fern protein on whitefly which revealed the lowest fecundity in the transgenic line C across F0,F1 and F3 generations,lower egg hatching in F1 and F2 generations in transgenic lines A and B,shorter nymphal duration in F1 and F2 generations in transgenic line B,and the least total adult emergence in the transgenic line C in F0 and F3 generations.Conclusions These findings suggest that the transgenic cotton lines expressing fern protein disrupts whitefly populations and the life cycle to a certain extent.However,results are not consistent over generations and years of study,indicating these transgenic lines were not superior over control lines and need to be improved in future breeding. 展开更多
关键词 EVALUATION Fern protein Transgenic cotton lines WHITEFLY
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逆转座子LINE-1对293T细胞增殖及细胞形态的影响
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作者 李丹 刘红梅 张鹏 《中国生物工程杂志》 北大核心 2025年第7期1-10,共10页
目的:探索逆转座子LINE-1(long interspersed nuclear elements-1)编码的ORF-1p在维持293T细胞增殖和形态中的作用。方法:利用核糖核酸干扰(RNA interference,RNAi)技术,构建LINE-1 ORF-1p表达量下调的293T细胞系,通过qPCR(quantitative... 目的:探索逆转座子LINE-1(long interspersed nuclear elements-1)编码的ORF-1p在维持293T细胞增殖和形态中的作用。方法:利用核糖核酸干扰(RNA interference,RNAi)技术,构建LINE-1 ORF-1p表达量下调的293T细胞系,通过qPCR(quantitative polymerase chain reaction)和Western blot验证干扰效率,通过免疫荧光染色检测ORF-1p的表达和定位,通过细胞计数和克隆形成实验检测细胞增殖能力,通过鬼笔环肽和4’,6-二脒基-2-苯基吲哚(4’,6-diamidino-2-phenylindole,DAPI)染色分析细胞形态。结果:成功构建两种LINE-1敲低的293T细胞系,通过免疫荧光染色观察ORF-1p的表达定位,显示ORF-1p主要分布在细胞质,干扰LINE-1后ORF-1p表达减少;通过细胞计数和集落形成实验,发现下调ORF-1p后细胞的增殖能力和克隆形成能力显著降低(P<0.05);通过鬼笔环肽标记细胞骨架,DAPI标记细胞核,相差显微镜和共聚焦显微镜观察干扰LINE-1后细胞形态,结果显示,下调ORF-1p后shL1-749细胞面积减小,细胞圆润度增加(P<0.05)。结论:成功构建了LINE-1敲低的293T细胞系,发现下调ORF-1p蛋白能够抑制293T细胞的生长,改变细胞形态,为进一步了解LINE-1在细胞增殖和调控中的分子机制奠定了基础。 展开更多
关键词 line-1 ORF-1p RNAI 293T 细胞增殖 细胞形态
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