[Objectives]To explore the pharmacodynamic effect of the Castanopsis sclerophylla(Lindl)Schottk fruit on diarrhea model mice.[Methods]The anti-diarrheal efficacy of the C.sclerophylla fruit was evaluated using a casto...[Objectives]To explore the pharmacodynamic effect of the Castanopsis sclerophylla(Lindl)Schottk fruit on diarrhea model mice.[Methods]The anti-diarrheal efficacy of the C.sclerophylla fruit was evaluated using a castor oil-induced acute diarrhea model in mice and the small intestinal charcoal propulsion test,with assessment of onset time of diarrhea,frequency of loose stools,and small intestinal propulsion rate.[Results]Compared with the model group,the C.sclerophylla frui-treated group demonstrated significantly delayed onset of diarrhea,reduced frequency of loose stools,and decreased small intestinal charcoal propulsion rate in a dose-dependent manner,with the high-dose group achieving efficacy comparable to the loperamide-treated group.[Conclusions]The C.sclerophylla fruit has certain anti-diarrhea effect,and its anti-diarrhea mechanism may be related to the inhibition of small intestinal peristalsis.展开更多
[Objective] This research aimed to establish an inductively coupled plasma mass spectrometry （ICP-MS） method for determination of five kinds of heavy metals （Pb, Cd, Cu, As and Hg） in Dendrobium candidium Wall. ex...[Objective] This research aimed to establish an inductively coupled plasma mass spectrometry （ICP-MS） method for determination of five kinds of heavy metals （Pb, Cd, Cu, As and Hg） in Dendrobium candidium Wall. ex Lindl. [Method] The samples were digested in HNO3-H202 acids system by closed vessel microwave di- gestion. At the same time, internal standard was added to avoid the matrix effect. [Result] The five kinds of heavy meals all showed good linear relationships, and the correlation coefficients ranged from 0.998 2 to 0.999 9. The detection limits of the five kinds of heavy metals were in the range of 0.008-0.750 μg/L, while the stan- dard recovery rates were in the range of 90.4%-96.9%. The relative standard devi- ations ranged from 1.6% to 8.2%. The heavy metals in the Ginseng and Astragalus national standard samples were determined by the established ICP-MS method, and the results were in conformity with the standard values. [Conclusion] The established method has simple and convenient operation and accurate and reliable results, and it meets the requirements by determination of heavy metals in Dendrobium candididium Wall. ex Lindl.展开更多
Dendrobium nobile Lindl.alkaloids（DNLA）,the active ingredients of a traditional Chinese medicine Dendrobium,have been shown to have anti-oxidative effects,anti-inflammatory action,and protective effect on neurons ag...Dendrobium nobile Lindl.alkaloids（DNLA）,the active ingredients of a traditional Chinese medicine Dendrobium,have been shown to have anti-oxidative effects,anti-inflammatory action,and protective effect on neurons against oxygen-glucose deprivation.However,it is not clear whether DNLA reduces amyloid-beta（Aβ）-induced neuronal injury.In this study,cortical neurons were treated with DNLA at different concentrations（0.025,0.25,and 2.5 mg/L）for 24 hours,followed by administration of Aβ（25-35）（10μM）.Aβ（25-35） treatments increased cell injury as determined by the leakage of lactate dehydrogenase,which was accompanied by chromatin condensation and mitochondrial tumefaction.The damage caused by Aβ（25-35） on these cellular properties was markedly attenuated when cells were pretreated with DNLA.Treatment with Aβ（25-35）down-regulated the expressions of postsynaptic density-95 mRNA and decreased the protein expression of synaptophysin and postsynaptic density-95,all changes were significantly reduced by pretreatment of cells with DNLA.These findings suggest that DNLA reduces the cytotoxicity induced by Aβ（25-35） in rat primary cultured neurons.The protective mechanism that DNLA confers on the synaptic integrity of cultured neurons might be mediated,at least in part,through the upregulation of neurogenesis related proteins synaptophysin and postsynaptic density-95.展开更多
The effect of La 3+ and Eu 3+ on the rooting of Eriobotrya japonica Lindl. plantlet in vitro was studied with adding La 3+ and Eu 3+ to the rooted medium. The rooting rate, the number of root and the lengt...The effect of La 3+ and Eu 3+ on the rooting of Eriobotrya japonica Lindl. plantlet in vitro was studied with adding La 3+ and Eu 3+ to the rooted medium. The rooting rate, the number of root and the length of root were studied after transplanting 20 d. The activity of peroxidase, nitrate reductase and fresh weight of roots were determined after transplanting 44 d. The results show that the optimum concentration range of La 3+ (1.0～3.0 μmol·L -1), Eu 3+ (2.0～3.0 μmol·L -1) in the rooted medium can increase the rooting rate and the fresh weight of roots, and promote the length of root and raise the activities of peroxidase and nitrate reductase significantly. La 3+ has more effect in improving the rooting rate, root length and the activities of peroxidase and less effect in promoting root fresh weight and the activities of nitrate reductase than Eu 3+.展开更多
[Objectives]This study was conducted to study the total polyphenol content and antioxidant capacity of different extraction fractions of Pholidota chinensis.[Methods]Different polar fractions of P.chinensis were obtai...[Objectives]This study was conducted to study the total polyphenol content and antioxidant capacity of different extraction fractions of Pholidota chinensis.[Methods]Different polar fractions of P.chinensis were obtained by ultrasonic extraction method,and determined for polyphenol contents by UV spectrophotometry,and their antioxidant activity was evaluated by DPPH free radical scavenging and ferric ion reducing antioxidant power(FRAP).SPSS analysis software was used to analyze the correlation between total polyphenol contents and antioxidant activity of different extracts.[Results]The contents of total polyphenols in different polar fractions ranged from 1.52 to 6.41 mg/g,and the order from low to high was:water<petroleum ether<ethyl acetate<95%ethanol.The reduction capacity of the total antioxidant capacity method ranged from 12.54 to 22.45μmol/L,and the order was 95%ethanol>ethyl acetate>petroleum ether>water.The DPPH median inhibition concentration ranged from 0.35 to 2.65 mg/ml,and the order was water>petroleum ether>ethyl acetate>95%ethanol.The total polyphenol content of P.chinensis was best in the 95%ethanol extraction fraction,which showed the strongest total antioxidant capacity,so the total polyphenol content of the 95%ethanol fraction was positively correlated with its antioxidant capacity,while the DPPH free radical-scavenging capacity was better in the water fraction,indicating that the free radical-scavenging capacity of the water fraction was better.[Conclusions]This study provides a theoretical basis for the further study of the chemical components of P.chinensis and the development and utilization of natural antioxidants.展开更多
One pot rapid and green bio-synthesis of stable fluorescent silver nanoparticles (Ag-NPs) from silver nitrate solution using root extract of Gymnadenia orchidis Lindlat at ambient temperature is demonstrated productiv...One pot rapid and green bio-synthesis of stable fluorescent silver nanoparticles (Ag-NPs) from silver nitrate solution using root extract of Gymnadenia orchidis Lindlat at ambient temperature is demonstrated productively. Surface Plasmon resonance of the synthesized Ag-NPs was shown to occur at 439 nm and two sharp fluorescence peaks at 610 and 780 nm were observed. FTIR study publicized the presence of chemically functional groups in the root extract surrounding the Ag-NPs, acting as stabilizers. XRD and FESEM analysis revealed that Ag-NPs were face centred cubic structure being spherical in shape with an average particle size of 28 ± 2 nm (n = 100). The stability of Ag-NPs in neutral pH environment was confirmed by Zeta Potential (ζ) behaviour. The Ag-NPs showed superior antioxidant activity. The notable photo-catalytic activity of biosynthesized Ag-NPs attributed to the existence of some proteins, responsible for degradation of methylene blue dye. Furthermore, Ag-NPs were found to exhibit a significant antibacterial effect against gram positive Staphylococcus aureus (S. aureus) bacteria.展开更多
OBJECTIVE Activation of the PINK1/Parkin-mediated autophagy pathway has been proposed to play a protective role in the development of neurological disorders through the elimination of damaged macromolecules or organel...OBJECTIVE Activation of the PINK1/Parkin-mediated autophagy pathway has been proposed to play a protective role in the development of neurological disorders through the elimination of damaged macromolecules or organelles.Exposure to excessive manganese(Mn) causes neurotoxicity and can produce a Parkinson disease(PD)-like neurological disorder.Mitochondrial dysfunction and oxidative stress are implicated in the mechanism of Mn induced neurotoxicity.The present study was designed to determine whether Dendrobium nobile Lindl.alkaloids(DNLA),a Chinese medicinal herb extract,confers protective function over Mn-induced cell toxicity,and to investigate whether the modulation of PINK1/Parkin-mediated autophagy is involved in the mechanism of DNLA-mediated cell protection over Mn toxicity.METHODS AND RESULTS Rat adrenal pheochromocytoma PC12 cells were utilized as an in vitro model of Mn cell toxicity.It was found that the treatment of the PC12 cells with Mn resulted in concentration-dependent cell death,accompanied by a decrease in mitochondrial respiration capacity and an increase in ROS generation,whereas pretreatment of cells with DNLA significantly alleviated cell toxicity induced by Mn and improved mitochondrial function and oxidative status.Mn treatment enhanced apoptotic cells along with a marked increase in the protein expression of Bax and a decrease in the expression of Bcl-2 protein.On the contrary,DNLA increased Bcl-2 expression,and concomitantly dramatically decreased the Bax/Bcl-2 ratio.Analysis of the expression of PINK1 and Parkin revealed that pretreatment of cells with DNLA significantly alleviated the decrease in protein levels of both PINK1 and Parkin caused by Mn.Furthermore,cells treated with Mn exhibited increased expression of LC3-Ⅱ and a decrease in accumulation of P62,which was noticeably reversed by the pretreatment of cells with DNLA.CONCLUSION DNLA inhibits Mn induced cytotoxicity,which may be mediated through modulating PINK1/Parkin-mediated autophagic flux and improving mitochondrial function.展开更多
Pholidota chinensis Lindl. is a plant in Pholidota of Orchidaceae family~[1],as well as a traditional edible and medicinal plant in Guangxi Province,and has the effects of clearing away heat and nourishing yin,and pre...Pholidota chinensis Lindl. is a plant in Pholidota of Orchidaceae family~[1],as well as a traditional edible and medicinal plant in Guangxi Province,and has the effects of clearing away heat and nourishing yin,and preventing phlegm form forming and stopping coughing. In this paper,the research on the herbal medicine,medicinal identification,chemical composition and extraction process of some chemical components,trace elements,clinical application and pharmacological action,antibacterial effect were reviewed. The existing problems in the current research were analyzed,and its future research direction were prospected,with an attempt to provide reference for its future research and development and utilization.展开更多
Biological characteristics of Alternaria tenuissima on Dendrobium sandidium Wall. et Lindl. were studied. The result showed that A. tenuissima on D. sandidium Wall. et Lindl. could grow at temperature 5 -35 ℃and pH v...Biological characteristics of Alternaria tenuissima on Dendrobium sandidium Wall. et Lindl. were studied. The result showed that A. tenuissima on D. sandidium Wall. et Lindl. could grow at temperature 5 -35 ℃and pH value of 2 - 10, the optimal temperature and pH value were 25℃ and 7 for its growth and sporulation, respectively. Starch was the optimal carbon sources and peptone was the optimal nitrogen source for the growth and sporulation of bacterial colony, re- spectively; PSA and PCA were the optimal media for its growth and sporalation, respectively.展开更多
Many forest ecosystems in Kenya are at risk from the invasion of exotic plant species that pose numerous threats like decreasing biodiversity, deteriorating ecosystem processes and degrading their services. They also ...Many forest ecosystems in Kenya are at risk from the invasion of exotic plant species that pose numerous threats like decreasing biodiversity, deteriorating ecosystem processes and degrading their services. They also affect human, other animal health and various angles of the general economy. Cestrum aurantiacum Lindl. is a species with invasive reputation having been reported with very high biological success rating and has been noted in parts of Kenya in proportions that raise concerns. It has negative effects on other plants and animal species function and diversity. These impacts have been recorded in Kenya and elsewhere in this review, I synthesized data from studies that have examined the taxonomy of Cestrum aurantiacum, how this species become part of the ecosystem in Kenya and its ecological and economic impacts. I sourced relevant articles from the internet using keywords relating to the taxonomy, impacts and reports of invasive species and narrowed to records from different parts of Kenya. In this review, I looked across twenty-seven studies, on Cestrum aurantiacum in Kenya and went ahead to review one hundred and thirteen other articles for expanded discussion. Species data in published articles from different parts of Kenya were used as georefences to model overall species distribution which was noted to lie between Mt Kenya, Mt Elgon-Nandi hills and Cherangani hills. It was clearly established that there are major adverse effects associated with species. First, it can change native ecosystem processes such as nutrient cycle or hydrology and contribute significant role on the decrease of native species. A primary risk of Cestrum aurantiacum is that when this species alters the biodiversity, ecosystems are transformed into new configurations with unpredictable consequences to humans and other wildlife in totality. Despite the few positive applications of cestrum species, these can’t compensate for the enormous detrimental consequences associated with the species. Cestrum aurantiacum was introduced in Kenya as an ornamental plant in 1921 by the government and was distributed in several regions of high potential Kenya due to high level of hybridization within the genera, several variables of the species occur. By examining the level of information regarding the taxonomy and impacts of Cestrum aurantiacum on animal and plant species, this study provides pivotal information at the country level with a view to informing monitoring and conservation efforts, such as alien plant removal and control programmes, and ensuring that endemic terrestrial animal and plant diversity are maintained.展开更多
[Objectives]To improve the yield of flavonoids from Dendrobium nobile Lindl.[Methods]On the basis of four single-factor experiments of solid/liquid ratio,extraction temperature,extraction time and ethanol volume fract...[Objectives]To improve the yield of flavonoids from Dendrobium nobile Lindl.[Methods]On the basis of four single-factor experiments of solid/liquid ratio,extraction temperature,extraction time and ethanol volume fraction,the ultrasonic-assisted extraction process of total flavonoids from D.nobile Lindl.was optimized using quadratic general rotary unitized design,and the in-vitro anti-oxidant activity of the flavonoids extracted was evaluated preliminarily.[Results]The optimized extraction process for flavonoids in D.nobile Lindl.was as follows:extraction time 10 min,solid/liquid ratio 1∶15(g∶mL),extraction temperature 75℃and ethanol volume fraction 90%.According to the revised optimal process,6 repeated experiments were carried out,and the average yield of flavonoids was(0.5515±0.0004)mg/g,consistent with the predicted value of the model.The results of anti-oxidation experiment show that the flavonoids extracted from D.nobile Lindl.have better scavenging effect on ABTS and DPPH free radicals,and the scavenging activity is proportional to the mass concentration of the extract.[Conclusions]The optimized extraction process for flavonoids from D.nobile Lindl.is reasonable and feasible,and the flavonoids in D.nobile Lindl.have strong antioxidant activity.展开更多
Dendrobium nobile Lindl (D. nobile), a well-known precious herb, has a long history of use as a medicine and health food in China. Phytochemically, D. nobile has been found to contain various bioactive compounds, such...Dendrobium nobile Lindl (D. nobile), a well-known precious herb, has a long history of use as a medicine and health food in China. Phytochemically, D. nobile has been found to contain various bioactive compounds, such as alkaloids, bibenzyl, phenanthrene, phenylpropanoids, and polysaccharides. Its medicinal applications are closely correlated to its diverse pharmacological activities, including antitumor, anti-inflammatory, nervous system protective, antifatigue, hypoglycemic, and hypolipidemic actions. In this review, we provide a comprehensive summary of the main chemical constituents and pharmacological activities of D. nobile, as well as the underlying molecular mechanisms for its bioactivities. It is expected that this review will provide a helpful scientific reference for the development and use of D. nobile.展开更多
[Objectives] To study the original plant,Crude herb and microscopic identification of Yi Crude herb Pholidota chinensis Lindl.,and provide experimental data for identification and application. [Methods]Plant identific...[Objectives] To study the original plant,Crude herb and microscopic identification of Yi Crude herb Pholidota chinensis Lindl.,and provide experimental data for identification and application. [Methods]Plant identification,character identification,powder identification and paraffin section identification were adopted in the study. [Results]The characteristics of Yi Crude herb of Pholidota chinensis Lindl. were obvious in the original plant,Crude herb and microscopic characteristics. [Conclusions]This paper provided reference for developing quality standards of Yi Crude herb Pholidota chinensis Lindl. and original plant identification,and provided the basis for further research.展开更多
Hovenia acerba Lindl.(HA)was formerly used as a treatment for alcohol overdosing.To explore the mechanism of hepatoprotective effect of HA,the chemical components of Hovenia acerba Lindl.peduncles extract(HAPE)and Hov...Hovenia acerba Lindl.(HA)was formerly used as a treatment for alcohol overdosing.To explore the mechanism of hepatoprotective effect of HA,the chemical components of Hovenia acerba Lindl.peduncles extract(HAPE)and Hovenia acerba Lindl.seeds extract(HASE)were compared by UPLC-QTOF-MS.Moreover,the alcohol damage model of human normal hepatocytes(LO2)and male Kunming mice were used to measure their effects on alcoholic liver damage.Results showed that HASE had 32 kinds of phenolic compounds,and the main components were caffeic acid,vitexin-2′′-O-glucoside,ferulic acid hexoside,quercetin and its glycosides;HAPE had 24 kinds of phenolic compounds,and the main components were caffeic acid,kaempferol and chrysophano.The phenolic content of HASE(151.73±12.66 mg gallic acid equivalent per gram of dry weight)(mg GAE/g DW)was higher than that of HAPE(17.89±1.59 mg GAE/g DW).HASE and HAPE could increase the LO2 cell viability in the alcohol damage models.Moreover,the levels of triacylglycerol(TG),total cholesterol(TC),alanine aminotransferase(ALT),and aspartate aminotransferase(AST)were decreased,the activities of superoxide dismutase(SOD)and catalase(CAT)were increased,and the concentration of malondialdehyde(MDA)was decreased in both HASE and HAPE treated cell and mice groups.Results also showed that the concentration of reduced glutathione(GSH)and the activity of glutathione peroxidase(GSH-Px)were increased in mice.HASE and HAPE raised the mRNA expressions of nuclear factor erythroid-2 related factor 2(Nrf2)and activated antioxidant-related genes:heme oxygenase-1(HO-1),NADPH quinone acceptor oxidoreductase 1(NQO1),and glutamate-cysteine ligase modifier subunit(GCLM),and enhanced the expression of Nrf2 and HO-1 proteins.HASE and HAPE could also increase antioxidant activity by enhancing Nrf2-mediated inducible expression of HO-1 to protect hepatocytes from injury.Furthermore,HASE showed better liver protective effect than HAPE.This study demonstrated the protective benefits of Hovenia acerba Lindl.peduncles and seeds extracts on alcoholic liver injury.展开更多
Objective Anoectochilus roxburghii is a valuable medicinal and ornamental plant.The aim of this study is to investigate the morphological and biochemical responses during the flower development stages of A.roxburghii,...Objective Anoectochilus roxburghii is a valuable medicinal and ornamental plant.The aim of this study is to investigate the morphological and biochemical responses during the flower development stages of A.roxburghii,and to assess the effects of exogenous polyamines(PAs)on bud differentiation and metabolism,thereby providing a theoretical basis for understanding the flowering form and physiology of A.roxburghii.Methods In this study,morphological and biochemical responses in flower development stages of A.roxburghii were investigated using paraffin sections and stereomicroscope.A.roxburghii was divided into five periods,including vegetative growth period,flower bud period,flowering period,late flowering period and fruiting period.During the flowering phase,specific biochemical parameters were measured,including soluble sugar content,superoxide dismutase(SOD)activity,soluble protein content,peroxidase(POD)activity,and catalase(CAT)activity.These measurements were conducted to understand the biochemical changes occurring within A.roxburghii during its flowering process.Furthermore,the effects of PAs on bud differentiation were examined.Additionally,the activities of S-adenosylmethionine decarboxylase(SAMDC)and polyamine oxidase(PAO),as well as the content of polyphenols,polysaccharides,and flavonoids in A.roxburghii,were measured after PA treatment to evaluate the metabolic changes induced by exogenous PAs.Results During the flowering phase of A.roxburghii,soluble sugar content and SOD activity were steadily declining.Soluble protein content was initially increasing and then reducing,and POD and CAT activities showed opposite pattern.In addition,the effects of exogenous PAs on bud differentiation were investigated.Results showed that 3 mmol/L putrescine or 0.3 mmol/L spermidine significantly promoted the bud differentiation of A.roxburghii and advanced the flowering.The activities of SAMDC,PAO,and the content of polyphenols,polysaccharides and flavonoids in A.roxburghii significantly increased after PA treatment,demonstrating that exogenous PA can accelerate metabolism and improved the active ingredients content.Conclusion The flower development of A.roxburghii was divided into five stages,with significant changes in soluble sugar,protein,POD,SOD,CAT,MDA,and PRO levels.Exogenous putrescine and spermidine enhanced bud differentiation and accelerated flowering,increasing SAMDC and PAO activities,suggesting accelerated PA metabolism.PAs also improved active component content.These findings provide a theoretical basis for studying flower morphology and PA-induced flowering regulation of A.roxburghii.展开更多
基金Supported by Zhongshan City Medical Research Project(2023A020437).
文摘[Objectives]To explore the pharmacodynamic effect of the Castanopsis sclerophylla(Lindl)Schottk fruit on diarrhea model mice.[Methods]The anti-diarrheal efficacy of the C.sclerophylla fruit was evaluated using a castor oil-induced acute diarrhea model in mice and the small intestinal charcoal propulsion test,with assessment of onset time of diarrhea,frequency of loose stools,and small intestinal propulsion rate.[Results]Compared with the model group,the C.sclerophylla frui-treated group demonstrated significantly delayed onset of diarrhea,reduced frequency of loose stools,and decreased small intestinal charcoal propulsion rate in a dose-dependent manner,with the high-dose group achieving efficacy comparable to the loperamide-treated group.[Conclusions]The C.sclerophylla fruit has certain anti-diarrhea effect,and its anti-diarrhea mechanism may be related to the inhibition of small intestinal peristalsis.
文摘[Objective] This research aimed to establish an inductively coupled plasma mass spectrometry （ICP-MS） method for determination of five kinds of heavy metals （Pb, Cd, Cu, As and Hg） in Dendrobium candidium Wall. ex Lindl. [Method] The samples were digested in HNO3-H202 acids system by closed vessel microwave di- gestion. At the same time, internal standard was added to avoid the matrix effect. [Result] The five kinds of heavy meals all showed good linear relationships, and the correlation coefficients ranged from 0.998 2 to 0.999 9. The detection limits of the five kinds of heavy metals were in the range of 0.008-0.750 μg/L, while the stan- dard recovery rates were in the range of 90.4%-96.9%. The relative standard devi- ations ranged from 1.6% to 8.2%. The heavy metals in the Ginseng and Astragalus national standard samples were determined by the established ICP-MS method, and the results were in conformity with the standard values. [Conclusion] The established method has simple and convenient operation and accurate and reliable results, and it meets the requirements by determination of heavy metals in Dendrobium candididium Wall. ex Lindl.
基金financially supported by the National Natural Science Foundation of China,No.81473201the Natural Science Foundation of Educational Commission of Guizhou Province of China,No.2010043+1 种基金the Science and Technology Foundation of Guizhou Province of China,No.JZ[2014]2016the Modernization of Traditional Chinese Medicine Project of Guizhou Province of China,No.[2011]5086
文摘Dendrobium nobile Lindl.alkaloids（DNLA）,the active ingredients of a traditional Chinese medicine Dendrobium,have been shown to have anti-oxidative effects,anti-inflammatory action,and protective effect on neurons against oxygen-glucose deprivation.However,it is not clear whether DNLA reduces amyloid-beta（Aβ）-induced neuronal injury.In this study,cortical neurons were treated with DNLA at different concentrations（0.025,0.25,and 2.5 mg/L）for 24 hours,followed by administration of Aβ（25-35）（10μM）.Aβ（25-35） treatments increased cell injury as determined by the leakage of lactate dehydrogenase,which was accompanied by chromatin condensation and mitochondrial tumefaction.The damage caused by Aβ（25-35） on these cellular properties was markedly attenuated when cells were pretreated with DNLA.Treatment with Aβ（25-35）down-regulated the expressions of postsynaptic density-95 mRNA and decreased the protein expression of synaptophysin and postsynaptic density-95,all changes were significantly reduced by pretreatment of cells with DNLA.These findings suggest that DNLA reduces the cytotoxicity induced by Aβ（25-35） in rat primary cultured neurons.The protective mechanism that DNLA confers on the synaptic integrity of cultured neurons might be mediated,at least in part,through the upregulation of neurogenesis related proteins synaptophysin and postsynaptic density-95.
文摘The effect of La 3+ and Eu 3+ on the rooting of Eriobotrya japonica Lindl. plantlet in vitro was studied with adding La 3+ and Eu 3+ to the rooted medium. The rooting rate, the number of root and the length of root were studied after transplanting 20 d. The activity of peroxidase, nitrate reductase and fresh weight of roots were determined after transplanting 44 d. The results show that the optimum concentration range of La 3+ (1.0～3.0 μmol·L -1), Eu 3+ (2.0～3.0 μmol·L -1) in the rooted medium can increase the rooting rate and the fresh weight of roots, and promote the length of root and raise the activities of peroxidase and nitrate reductase significantly. La 3+ has more effect in improving the rooting rate, root length and the activities of peroxidase and less effect in promoting root fresh weight and the activities of nitrate reductase than Eu 3+.
基金Supported by Guangxi Science and Technology Base and Talent Project(GK AD21238031)Guangxi Key Laboratory of Zhuang and Yao Ethnic Medicine(GKJZ[2014]32)+8 种基金Collaborative Innovation Center of Zhuang and Yao Ethnic Medicine(GJKY[2013]20)Guangxi Engineering Research Center of Ethnic Medicine Resources and Application(Guifa Gai High Technology Letter[2020]No.2605)The Eighth Batch of Guangxi Specially-employed Expert Projects(GRCTZ[2019]13)Youth Fund Project of Guangxi University of Chinese Medicine(2019QN012)Guangxi Key Research and Development Project(GK AB21196016)National Natural Science Foundation of China(82060695)Guangxi Zhuang Autonomous Region Philosophy and Social Science Planning Research Project(20BMZ005)Foreign Expert Project of the Ministry of Science and Technology of the People s Republic of China(GXL20200233001)Guangxi First-class Discipline of Traditional Chinese Medicine:Ethnic Medicine(GJKY[2018]12).
文摘[Objectives]This study was conducted to study the total polyphenol content and antioxidant capacity of different extraction fractions of Pholidota chinensis.[Methods]Different polar fractions of P.chinensis were obtained by ultrasonic extraction method,and determined for polyphenol contents by UV spectrophotometry,and their antioxidant activity was evaluated by DPPH free radical scavenging and ferric ion reducing antioxidant power(FRAP).SPSS analysis software was used to analyze the correlation between total polyphenol contents and antioxidant activity of different extracts.[Results]The contents of total polyphenols in different polar fractions ranged from 1.52 to 6.41 mg/g,and the order from low to high was:water<petroleum ether<ethyl acetate<95%ethanol.The reduction capacity of the total antioxidant capacity method ranged from 12.54 to 22.45μmol/L,and the order was 95%ethanol>ethyl acetate>petroleum ether>water.The DPPH median inhibition concentration ranged from 0.35 to 2.65 mg/ml,and the order was water>petroleum ether>ethyl acetate>95%ethanol.The total polyphenol content of P.chinensis was best in the 95%ethanol extraction fraction,which showed the strongest total antioxidant capacity,so the total polyphenol content of the 95%ethanol fraction was positively correlated with its antioxidant capacity,while the DPPH free radical-scavenging capacity was better in the water fraction,indicating that the free radical-scavenging capacity of the water fraction was better.[Conclusions]This study provides a theoretical basis for the further study of the chemical components of P.chinensis and the development and utilization of natural antioxidants.
文摘One pot rapid and green bio-synthesis of stable fluorescent silver nanoparticles (Ag-NPs) from silver nitrate solution using root extract of Gymnadenia orchidis Lindlat at ambient temperature is demonstrated productively. Surface Plasmon resonance of the synthesized Ag-NPs was shown to occur at 439 nm and two sharp fluorescence peaks at 610 and 780 nm were observed. FTIR study publicized the presence of chemically functional groups in the root extract surrounding the Ag-NPs, acting as stabilizers. XRD and FESEM analysis revealed that Ag-NPs were face centred cubic structure being spherical in shape with an average particle size of 28 ± 2 nm (n = 100). The stability of Ag-NPs in neutral pH environment was confirmed by Zeta Potential (ζ) behaviour. The Ag-NPs showed superior antioxidant activity. The notable photo-catalytic activity of biosynthesized Ag-NPs attributed to the existence of some proteins, responsible for degradation of methylene blue dye. Furthermore, Ag-NPs were found to exhibit a significant antibacterial effect against gram positive Staphylococcus aureus (S. aureus) bacteria.
文摘OBJECTIVE Activation of the PINK1/Parkin-mediated autophagy pathway has been proposed to play a protective role in the development of neurological disorders through the elimination of damaged macromolecules or organelles.Exposure to excessive manganese(Mn) causes neurotoxicity and can produce a Parkinson disease(PD)-like neurological disorder.Mitochondrial dysfunction and oxidative stress are implicated in the mechanism of Mn induced neurotoxicity.The present study was designed to determine whether Dendrobium nobile Lindl.alkaloids(DNLA),a Chinese medicinal herb extract,confers protective function over Mn-induced cell toxicity,and to investigate whether the modulation of PINK1/Parkin-mediated autophagy is involved in the mechanism of DNLA-mediated cell protection over Mn toxicity.METHODS AND RESULTS Rat adrenal pheochromocytoma PC12 cells were utilized as an in vitro model of Mn cell toxicity.It was found that the treatment of the PC12 cells with Mn resulted in concentration-dependent cell death,accompanied by a decrease in mitochondrial respiration capacity and an increase in ROS generation,whereas pretreatment of cells with DNLA significantly alleviated cell toxicity induced by Mn and improved mitochondrial function and oxidative status.Mn treatment enhanced apoptotic cells along with a marked increase in the protein expression of Bax and a decrease in the expression of Bcl-2 protein.On the contrary,DNLA increased Bcl-2 expression,and concomitantly dramatically decreased the Bax/Bcl-2 ratio.Analysis of the expression of PINK1 and Parkin revealed that pretreatment of cells with DNLA significantly alleviated the decrease in protein levels of both PINK1 and Parkin caused by Mn.Furthermore,cells treated with Mn exhibited increased expression of LC3-Ⅱ and a decrease in accumulation of P62,which was noticeably reversed by the pretreatment of cells with DNLA.CONCLUSION DNLA inhibits Mn induced cytotoxicity,which may be mediated through modulating PINK1/Parkin-mediated autophagic flux and improving mitochondrial function.
基金Supported by Zhuang Yao Medicines Collaborative Innovation Center(Gui JKY[2013]No.20)Guangxi Zhuang Yao Medicines Key Laboratory(Gui KJZ[2014]No.32)+1 种基金Guangxi Key Disciplines on Zhuang Medicine(Gui JKY[2013]No.16)Studies of Bagui Scholars on Innovation Theory and Efficacy of TCM
文摘Pholidota chinensis Lindl. is a plant in Pholidota of Orchidaceae family~[1],as well as a traditional edible and medicinal plant in Guangxi Province,and has the effects of clearing away heat and nourishing yin,and preventing phlegm form forming and stopping coughing. In this paper,the research on the herbal medicine,medicinal identification,chemical composition and extraction process of some chemical components,trace elements,clinical application and pharmacological action,antibacterial effect were reviewed. The existing problems in the current research were analyzed,and its future research direction were prospected,with an attempt to provide reference for its future research and development and utilization.
文摘Biological characteristics of Alternaria tenuissima on Dendrobium sandidium Wall. et Lindl. were studied. The result showed that A. tenuissima on D. sandidium Wall. et Lindl. could grow at temperature 5 -35 ℃and pH value of 2 - 10, the optimal temperature and pH value were 25℃ and 7 for its growth and sporulation, respectively. Starch was the optimal carbon sources and peptone was the optimal nitrogen source for the growth and sporulation of bacterial colony, re- spectively; PSA and PCA were the optimal media for its growth and sporalation, respectively.
文摘Many forest ecosystems in Kenya are at risk from the invasion of exotic plant species that pose numerous threats like decreasing biodiversity, deteriorating ecosystem processes and degrading their services. They also affect human, other animal health and various angles of the general economy. Cestrum aurantiacum Lindl. is a species with invasive reputation having been reported with very high biological success rating and has been noted in parts of Kenya in proportions that raise concerns. It has negative effects on other plants and animal species function and diversity. These impacts have been recorded in Kenya and elsewhere in this review, I synthesized data from studies that have examined the taxonomy of Cestrum aurantiacum, how this species become part of the ecosystem in Kenya and its ecological and economic impacts. I sourced relevant articles from the internet using keywords relating to the taxonomy, impacts and reports of invasive species and narrowed to records from different parts of Kenya. In this review, I looked across twenty-seven studies, on Cestrum aurantiacum in Kenya and went ahead to review one hundred and thirteen other articles for expanded discussion. Species data in published articles from different parts of Kenya were used as georefences to model overall species distribution which was noted to lie between Mt Kenya, Mt Elgon-Nandi hills and Cherangani hills. It was clearly established that there are major adverse effects associated with species. First, it can change native ecosystem processes such as nutrient cycle or hydrology and contribute significant role on the decrease of native species. A primary risk of Cestrum aurantiacum is that when this species alters the biodiversity, ecosystems are transformed into new configurations with unpredictable consequences to humans and other wildlife in totality. Despite the few positive applications of cestrum species, these can’t compensate for the enormous detrimental consequences associated with the species. Cestrum aurantiacum was introduced in Kenya as an ornamental plant in 1921 by the government and was distributed in several regions of high potential Kenya due to high level of hybridization within the genera, several variables of the species occur. By examining the level of information regarding the taxonomy and impacts of Cestrum aurantiacum on animal and plant species, this study provides pivotal information at the country level with a view to informing monitoring and conservation efforts, such as alien plant removal and control programmes, and ensuring that endemic terrestrial animal and plant diversity are maintained.
基金Key Science and Technology Program of Science&Technology Department of Henan Province(172102310211,182102110166,192102310087,202102310478)Key Scientific and Technological Research Project for Colleges and Universities(18A180026,20B350006)+1 种基金Starting Foundation of Pingdingshan University for High-level Talents(PXY-BSQD-2018011,PXY-BSQD-2018010)Cultivation Fund of Pingdingshan University(PXY-PYJJ-2019007)。
文摘[Objectives]To improve the yield of flavonoids from Dendrobium nobile Lindl.[Methods]On the basis of four single-factor experiments of solid/liquid ratio,extraction temperature,extraction time and ethanol volume fraction,the ultrasonic-assisted extraction process of total flavonoids from D.nobile Lindl.was optimized using quadratic general rotary unitized design,and the in-vitro anti-oxidant activity of the flavonoids extracted was evaluated preliminarily.[Results]The optimized extraction process for flavonoids in D.nobile Lindl.was as follows:extraction time 10 min,solid/liquid ratio 1∶15(g∶mL),extraction temperature 75℃and ethanol volume fraction 90%.According to the revised optimal process,6 repeated experiments were carried out,and the average yield of flavonoids was(0.5515±0.0004)mg/g,consistent with the predicted value of the model.The results of anti-oxidation experiment show that the flavonoids extracted from D.nobile Lindl.have better scavenging effect on ABTS and DPPH free radicals,and the scavenging activity is proportional to the mass concentration of the extract.[Conclusions]The optimized extraction process for flavonoids from D.nobile Lindl.is reasonable and feasible,and the flavonoids in D.nobile Lindl.have strong antioxidant activity.
文摘Dendrobium nobile Lindl (D. nobile), a well-known precious herb, has a long history of use as a medicine and health food in China. Phytochemically, D. nobile has been found to contain various bioactive compounds, such as alkaloids, bibenzyl, phenanthrene, phenylpropanoids, and polysaccharides. Its medicinal applications are closely correlated to its diverse pharmacological activities, including antitumor, anti-inflammatory, nervous system protective, antifatigue, hypoglycemic, and hypolipidemic actions. In this review, we provide a comprehensive summary of the main chemical constituents and pharmacological activities of D. nobile, as well as the underlying molecular mechanisms for its bioactivities. It is expected that this review will provide a helpful scientific reference for the development and use of D. nobile.
基金Supported by National Science and Technology Support Program(2015BAC05-B02)Sichuan Science and Technology Support Program(2015SZ0034)Education and Teaching Research and Reform Project of Southwest University for Nationalities(2015)
文摘[Objectives] To study the original plant,Crude herb and microscopic identification of Yi Crude herb Pholidota chinensis Lindl.,and provide experimental data for identification and application. [Methods]Plant identification,character identification,powder identification and paraffin section identification were adopted in the study. [Results]The characteristics of Yi Crude herb of Pholidota chinensis Lindl. were obvious in the original plant,Crude herb and microscopic characteristics. [Conclusions]This paper provided reference for developing quality standards of Yi Crude herb Pholidota chinensis Lindl. and original plant identification,and provided the basis for further research.
基金funded by the National Natural Science Foundation of China(Grant No.27964012).
文摘Hovenia acerba Lindl.(HA)was formerly used as a treatment for alcohol overdosing.To explore the mechanism of hepatoprotective effect of HA,the chemical components of Hovenia acerba Lindl.peduncles extract(HAPE)and Hovenia acerba Lindl.seeds extract(HASE)were compared by UPLC-QTOF-MS.Moreover,the alcohol damage model of human normal hepatocytes(LO2)and male Kunming mice were used to measure their effects on alcoholic liver damage.Results showed that HASE had 32 kinds of phenolic compounds,and the main components were caffeic acid,vitexin-2′′-O-glucoside,ferulic acid hexoside,quercetin and its glycosides;HAPE had 24 kinds of phenolic compounds,and the main components were caffeic acid,kaempferol and chrysophano.The phenolic content of HASE(151.73±12.66 mg gallic acid equivalent per gram of dry weight)(mg GAE/g DW)was higher than that of HAPE(17.89±1.59 mg GAE/g DW).HASE and HAPE could increase the LO2 cell viability in the alcohol damage models.Moreover,the levels of triacylglycerol(TG),total cholesterol(TC),alanine aminotransferase(ALT),and aspartate aminotransferase(AST)were decreased,the activities of superoxide dismutase(SOD)and catalase(CAT)were increased,and the concentration of malondialdehyde(MDA)was decreased in both HASE and HAPE treated cell and mice groups.Results also showed that the concentration of reduced glutathione(GSH)and the activity of glutathione peroxidase(GSH-Px)were increased in mice.HASE and HAPE raised the mRNA expressions of nuclear factor erythroid-2 related factor 2(Nrf2)and activated antioxidant-related genes:heme oxygenase-1(HO-1),NADPH quinone acceptor oxidoreductase 1(NQO1),and glutamate-cysteine ligase modifier subunit(GCLM),and enhanced the expression of Nrf2 and HO-1 proteins.HASE and HAPE could also increase antioxidant activity by enhancing Nrf2-mediated inducible expression of HO-1 to protect hepatocytes from injury.Furthermore,HASE showed better liver protective effect than HAPE.This study demonstrated the protective benefits of Hovenia acerba Lindl.peduncles and seeds extracts on alcoholic liver injury.
基金supported by the National Natural Science Foundation of China(No.82373977,82173916)Wenzhou Major Science and Technology Innovation Projects(No.ZNF2023007)+1 种基金the“San Nong Jiu Fang”Science and Technology Cooperation Project of Zhejiang Province(No.2024SNJF039)Tongxiang Science and Technology Plan Project(No.202301017).
文摘Objective Anoectochilus roxburghii is a valuable medicinal and ornamental plant.The aim of this study is to investigate the morphological and biochemical responses during the flower development stages of A.roxburghii,and to assess the effects of exogenous polyamines(PAs)on bud differentiation and metabolism,thereby providing a theoretical basis for understanding the flowering form and physiology of A.roxburghii.Methods In this study,morphological and biochemical responses in flower development stages of A.roxburghii were investigated using paraffin sections and stereomicroscope.A.roxburghii was divided into five periods,including vegetative growth period,flower bud period,flowering period,late flowering period and fruiting period.During the flowering phase,specific biochemical parameters were measured,including soluble sugar content,superoxide dismutase(SOD)activity,soluble protein content,peroxidase(POD)activity,and catalase(CAT)activity.These measurements were conducted to understand the biochemical changes occurring within A.roxburghii during its flowering process.Furthermore,the effects of PAs on bud differentiation were examined.Additionally,the activities of S-adenosylmethionine decarboxylase(SAMDC)and polyamine oxidase(PAO),as well as the content of polyphenols,polysaccharides,and flavonoids in A.roxburghii,were measured after PA treatment to evaluate the metabolic changes induced by exogenous PAs.Results During the flowering phase of A.roxburghii,soluble sugar content and SOD activity were steadily declining.Soluble protein content was initially increasing and then reducing,and POD and CAT activities showed opposite pattern.In addition,the effects of exogenous PAs on bud differentiation were investigated.Results showed that 3 mmol/L putrescine or 0.3 mmol/L spermidine significantly promoted the bud differentiation of A.roxburghii and advanced the flowering.The activities of SAMDC,PAO,and the content of polyphenols,polysaccharides and flavonoids in A.roxburghii significantly increased after PA treatment,demonstrating that exogenous PA can accelerate metabolism and improved the active ingredients content.Conclusion The flower development of A.roxburghii was divided into five stages,with significant changes in soluble sugar,protein,POD,SOD,CAT,MDA,and PRO levels.Exogenous putrescine and spermidine enhanced bud differentiation and accelerated flowering,increasing SAMDC and PAO activities,suggesting accelerated PA metabolism.PAs also improved active component content.These findings provide a theoretical basis for studying flower morphology and PA-induced flowering regulation of A.roxburghii.
