Background:To investigate the effects of acupuncture on post-stroke limb spasm model rats and the underlying mechanism.Methods:A total of 50 Sprague-Dawley rats were randomly divided into three groups,Control group(10...Background:To investigate the effects of acupuncture on post-stroke limb spasm model rats and the underlying mechanism.Methods:A total of 50 Sprague-Dawley rats were randomly divided into three groups,Control group(10),Model group(20)and Zhishen Tiaoxing(ZSTX)acupuncture group(20).Middle cerebral artery occlusion was conducted in SD rats to establish post-stroke limb spasm rats,which were treated with ZSTX acupuncture.Behavioral assays were determined by the Narrow ally test,the limb muscle tension was detected by the BL-420S test system,and infarct volume was assessed after the cerebral infarction by 2,3,5-triphenyltetrazolium chloride staining.Heterogeneous neurotransmitterγ-aminobutyric acid(GABA)and its receptors GABAA and GABAB in the cerebral cortex of the infarct area were determined by immunofluorescence assay.The release of Trkb and K-Cl cotransporter isoform 2 was detected by an immunofluorescence double labeling study.Western Blot was utilized to measure the expression of BDNF and Trkb.Results:The results showed that the behavioral assays in post-stroke limb spasm rats were significantly improved by the treatment of ZSTX acupuncture.14 days of ZSTX acupuncture can effectively inhibit muscle tone and decrease Infarct volume,which was measured with BL-420S biological function experiment system and triphenyltetrazolium chloride.Meanwhile,the results of Double-Label Immunofluorescence Assays showed that ZSTX acupuncture improved the expression of GABA,GABAA,GABAB,BDNF,and K-Cl cotransporter isoform 2.Double-Label Immunofluorescence Assays and WB results showed that 14 days ZSTX acupuncture declined the expression of Trkb.Conclusions:Our results suggest that 14 days of ZSTX acupuncture can significantly improve post-stroke limb spasm.Meanwhile,the pathogenesis of post-stroke limb spasm and the efficacy of ZSTX acupuncture involve metabolic pathways of neurotransmitters,and electro-acupuncture can treat post-stroke limb spasm by regulating BDNF/Trkb-KCC2 signaling pathway.展开更多
Background:To apply CiteSpace software to the hotspot,trend and cutting-edge dynamics of limb spasm after stroke in nearly 20 years.Methods:From 2000 to 2018,spasming literature in the Web of Science database were sea...Background:To apply CiteSpace software to the hotspot,trend and cutting-edge dynamics of limb spasm after stroke in nearly 20 years.Methods:From 2000 to 2018,spasming literature in the Web of Science database were searched,and cooperative analysis of authors'national institutions was conducted by using CiteSpace software,keyword analysis and cluster analysis,citation analysis of authors'literature journals,and visual map.Results:2005 articles were retrieved,and the number of articles increased year by year,and the country with the most articles was the United States.Keyword analysis shows that mechanistic research and clinical treatment of spasticity are research hotspots in this field.The most posts are Northwestern Univ and Univ Verona.Conclusion:Through the CiteSpace software analysis of spasticity literature,it is found that the research focus is embodied in the nerve regeneration technology and the standardized clinical study of botulinum toxin type A application,which has certain reference significance for the future research direction of spasticity.展开更多
Objective： To investigate the clinical significance of treating post-apoplectic limb spasm by puncturing the acupoints of Governor Vessel as a main therapy. Methods： Twenty- nine patients with post-apoplectic limb s...Objective： To investigate the clinical significance of treating post-apoplectic limb spasm by puncturing the acupoints of Governor Vessel as a main therapy. Methods： Twenty- nine patients with post-apoplectic limb spasm were treated by puncturing the acupoints of Governor Vessel plus the acupoints adjacent to joints, together with another 29 patients treated by conventional acupoints for comparison of the curative effects. Results： Before treatment, there was no significant difference in Ashoworth limb tension score and Fugl-Meyer motor function score between the two groups of patients （P〉0.05）. After treatment, there was significant difference in tension score between the two groups （P〈0.05）. Motor function score significantly increased as compared with that before the treatment in both groups （P〈0.01）, but limb motor function improved more obviously in the treatment group than in the control group （P〈0.05）. Conclusion： To puncture the acupoints of Governor Vessel as a main therapy can significantly improve the curative effect in the treatment of post-apoplectic limb spasm than conventional acupuncture method.展开更多
Objective:To observe the effect of bilateral transcranial direct current stimulation(tDCS)on the brain functional network in patients with upper limb spasm after stroke.Methods:An electroencephalograph(EEG)was used to...Objective:To observe the effect of bilateral transcranial direct current stimulation(tDCS)on the brain functional network in patients with upper limb spasm after stroke.Methods:An electroencephalograph(EEG)was used to analyze the effect of the brain functional network before and after the intervention of bilateral tDCS in patients with secondary upper limb spasm after stroke.A total of 44 patients with upper limb spasm after stroke were randomly divided into a real stimulation group and a sham stimulation group.Bilateral tDCS interventions were carried out respectively.The parameters of the brain functional network measured by EEG and the cross-correlation coefficients between various leads before the experiment and at 5 days after the experiment were compared.Results:During the imagination of grasping a mineral water bottle,the degree of nodes in the real stimulation group after stimulation was higher than that before stimulation and that of the sham stimulation group at the same period(P=0.004);during the imagination of releasing the mineral water bottle,the clustering coefficient and global efficiency of nodes in the real stimulation group were both higher than those of the sham stimulation group at the same period(P=0.020,0.032).Before stimulation,the importance scores of the degree of nodes among various leads ranged from 0.562 to 2.081.Among them,the importance scores of Pz,Oz,FP1,FP2,F3,F4,P4,O1,O2,F8,T5,C3,and Fz were all>1.After stimulation,the importance scores of all leads were>1,ranging from 1.911 to 5.580.The real stimulation group had a more obvious effect on P3,O1,O2,Cz,Oz,T4,T6,Fz,FP1,and T5.Conclusion:Bilateral tDCS intervention can enhance the degree of nodes during the imagination of grasping in patients with upper limb spasm after stroke,and enhance the clustering coefficient and global efficiency of nodes during the imagination of releasing.The brain functional network mainly has a more obvious impact on the brain functional areas of the leads in the parietal lobe,occipital lobe,central midline,occipital midline,middle temporal,posterior temporal,parietal midline,and frontal pole regions.展开更多
目的探究头针电动捻转法联合电针拮抗肌穴位对急性脑梗死(acute cerebral infarction,ACI)恢复期下肢痉挛患者步态参数及平衡功能的影响。方法选取该院2021年6月-2022年12月的ACI恢复期下肢痉挛患者102例,采用随机对照法分为两组,每组5...目的探究头针电动捻转法联合电针拮抗肌穴位对急性脑梗死(acute cerebral infarction,ACI)恢复期下肢痉挛患者步态参数及平衡功能的影响。方法选取该院2021年6月-2022年12月的ACI恢复期下肢痉挛患者102例,采用随机对照法分为两组,每组51例。两组患者均接受常规康复训练治疗;对照组在康复训练基础上采用电针拮抗肌穴位进行治疗;试验组在对照组基础上联合头针电动捻转法进行治疗。比较两组治疗前后神经功能缺损程度[美国国立卫生研究院卒中量表(national institutes of health stroke scale,NIHSS)评分]、平衡能力[Berg平衡量表(berg balance scale,BBS)评分]、下肢运动功能[Fugl-Meyer运动功能评定量表(fugl-meyer motor assessment,FMA)]及生活质量[脑梗死专用生活质量量表(stroke specific quality of life scale,SS-QOL)评分]变化情况,治疗后步态参数[全身三维步态与运动分析系统评分(步行速度、步频、步幅、单支撑时间以及双支撑时间)]。结果治疗后两组BBS、FMA下肢功能维度评分、步行速度、步频、步幅、单支撑时间以及SS-QOL较治疗前均显著升高,且试验组显著高于对照组(P<0.05);治疗后两组NIHSS、双支撑时间较治疗前均显著降低,且试验组显著低于对照组(P<0.05)。结论采用头针电动捻转法联合电针拮抗肌穴位治疗能有效提升ACI恢复期患者下肢运动能力、平衡功能,从而改善其步态参数及生活质量,对促进预后康复大有裨益。展开更多
基金the National Key R&D Program of China(Grant No.2019YFC1709900)National Natural Science Foundation of China(Grant No.71804022)+1 种基金Natural Science Foundation of Henan province(Grant No.232300420256)Medical technologies R&D Program of Henan province(Grant No.LHGJ20220348).
文摘Background:To investigate the effects of acupuncture on post-stroke limb spasm model rats and the underlying mechanism.Methods:A total of 50 Sprague-Dawley rats were randomly divided into three groups,Control group(10),Model group(20)and Zhishen Tiaoxing(ZSTX)acupuncture group(20).Middle cerebral artery occlusion was conducted in SD rats to establish post-stroke limb spasm rats,which were treated with ZSTX acupuncture.Behavioral assays were determined by the Narrow ally test,the limb muscle tension was detected by the BL-420S test system,and infarct volume was assessed after the cerebral infarction by 2,3,5-triphenyltetrazolium chloride staining.Heterogeneous neurotransmitterγ-aminobutyric acid(GABA)and its receptors GABAA and GABAB in the cerebral cortex of the infarct area were determined by immunofluorescence assay.The release of Trkb and K-Cl cotransporter isoform 2 was detected by an immunofluorescence double labeling study.Western Blot was utilized to measure the expression of BDNF and Trkb.Results:The results showed that the behavioral assays in post-stroke limb spasm rats were significantly improved by the treatment of ZSTX acupuncture.14 days of ZSTX acupuncture can effectively inhibit muscle tone and decrease Infarct volume,which was measured with BL-420S biological function experiment system and triphenyltetrazolium chloride.Meanwhile,the results of Double-Label Immunofluorescence Assays showed that ZSTX acupuncture improved the expression of GABA,GABAA,GABAB,BDNF,and K-Cl cotransporter isoform 2.Double-Label Immunofluorescence Assays and WB results showed that 14 days ZSTX acupuncture declined the expression of Trkb.Conclusions:Our results suggest that 14 days of ZSTX acupuncture can significantly improve post-stroke limb spasm.Meanwhile,the pathogenesis of post-stroke limb spasm and the efficacy of ZSTX acupuncture involve metabolic pathways of neurotransmitters,and electro-acupuncture can treat post-stroke limb spasm by regulating BDNF/Trkb-KCC2 signaling pathway.
文摘Background:To apply CiteSpace software to the hotspot,trend and cutting-edge dynamics of limb spasm after stroke in nearly 20 years.Methods:From 2000 to 2018,spasming literature in the Web of Science database were searched,and cooperative analysis of authors'national institutions was conducted by using CiteSpace software,keyword analysis and cluster analysis,citation analysis of authors'literature journals,and visual map.Results:2005 articles were retrieved,and the number of articles increased year by year,and the country with the most articles was the United States.Keyword analysis shows that mechanistic research and clinical treatment of spasticity are research hotspots in this field.The most posts are Northwestern Univ and Univ Verona.Conclusion:Through the CiteSpace software analysis of spasticity literature,it is found that the research focus is embodied in the nerve regeneration technology and the standardized clinical study of botulinum toxin type A application,which has certain reference significance for the future research direction of spasticity.
文摘Objective： To investigate the clinical significance of treating post-apoplectic limb spasm by puncturing the acupoints of Governor Vessel as a main therapy. Methods： Twenty- nine patients with post-apoplectic limb spasm were treated by puncturing the acupoints of Governor Vessel plus the acupoints adjacent to joints, together with another 29 patients treated by conventional acupoints for comparison of the curative effects. Results： Before treatment, there was no significant difference in Ashoworth limb tension score and Fugl-Meyer motor function score between the two groups of patients （P〉0.05）. After treatment, there was significant difference in tension score between the two groups （P〈0.05）. Motor function score significantly increased as compared with that before the treatment in both groups （P〈0.01）, but limb motor function improved more obviously in the treatment group than in the control group （P〈0.05）. Conclusion： To puncture the acupoints of Governor Vessel as a main therapy can significantly improve the curative effect in the treatment of post-apoplectic limb spasm than conventional acupuncture method.
文摘Objective:To observe the effect of bilateral transcranial direct current stimulation(tDCS)on the brain functional network in patients with upper limb spasm after stroke.Methods:An electroencephalograph(EEG)was used to analyze the effect of the brain functional network before and after the intervention of bilateral tDCS in patients with secondary upper limb spasm after stroke.A total of 44 patients with upper limb spasm after stroke were randomly divided into a real stimulation group and a sham stimulation group.Bilateral tDCS interventions were carried out respectively.The parameters of the brain functional network measured by EEG and the cross-correlation coefficients between various leads before the experiment and at 5 days after the experiment were compared.Results:During the imagination of grasping a mineral water bottle,the degree of nodes in the real stimulation group after stimulation was higher than that before stimulation and that of the sham stimulation group at the same period(P=0.004);during the imagination of releasing the mineral water bottle,the clustering coefficient and global efficiency of nodes in the real stimulation group were both higher than those of the sham stimulation group at the same period(P=0.020,0.032).Before stimulation,the importance scores of the degree of nodes among various leads ranged from 0.562 to 2.081.Among them,the importance scores of Pz,Oz,FP1,FP2,F3,F4,P4,O1,O2,F8,T5,C3,and Fz were all>1.After stimulation,the importance scores of all leads were>1,ranging from 1.911 to 5.580.The real stimulation group had a more obvious effect on P3,O1,O2,Cz,Oz,T4,T6,Fz,FP1,and T5.Conclusion:Bilateral tDCS intervention can enhance the degree of nodes during the imagination of grasping in patients with upper limb spasm after stroke,and enhance the clustering coefficient and global efficiency of nodes during the imagination of releasing.The brain functional network mainly has a more obvious impact on the brain functional areas of the leads in the parietal lobe,occipital lobe,central midline,occipital midline,middle temporal,posterior temporal,parietal midline,and frontal pole regions.
文摘目的探究头针电动捻转法联合电针拮抗肌穴位对急性脑梗死(acute cerebral infarction,ACI)恢复期下肢痉挛患者步态参数及平衡功能的影响。方法选取该院2021年6月-2022年12月的ACI恢复期下肢痉挛患者102例,采用随机对照法分为两组,每组51例。两组患者均接受常规康复训练治疗;对照组在康复训练基础上采用电针拮抗肌穴位进行治疗;试验组在对照组基础上联合头针电动捻转法进行治疗。比较两组治疗前后神经功能缺损程度[美国国立卫生研究院卒中量表(national institutes of health stroke scale,NIHSS)评分]、平衡能力[Berg平衡量表(berg balance scale,BBS)评分]、下肢运动功能[Fugl-Meyer运动功能评定量表(fugl-meyer motor assessment,FMA)]及生活质量[脑梗死专用生活质量量表(stroke specific quality of life scale,SS-QOL)评分]变化情况,治疗后步态参数[全身三维步态与运动分析系统评分(步行速度、步频、步幅、单支撑时间以及双支撑时间)]。结果治疗后两组BBS、FMA下肢功能维度评分、步行速度、步频、步幅、单支撑时间以及SS-QOL较治疗前均显著升高,且试验组显著高于对照组(P<0.05);治疗后两组NIHSS、双支撑时间较治疗前均显著降低,且试验组显著低于对照组(P<0.05)。结论采用头针电动捻转法联合电针拮抗肌穴位治疗能有效提升ACI恢复期患者下肢运动能力、平衡功能,从而改善其步态参数及生活质量,对促进预后康复大有裨益。
