Ligusticum sinense cv.Chuanxiong(L.Chuanxiong),one of the widely used traditional Chinese medicines(TCM),is currently facing the problem of excessive cadmium(Cd)content.This problem has significantly affected the qual...Ligusticum sinense cv.Chuanxiong(L.Chuanxiong),one of the widely used traditional Chinese medicines(TCM),is currently facing the problem of excessive cadmium(Cd)content.This problem has significantly affected the quality and safety of L.Chuanxiong and become a vital factor restricting its clinical application and international trade development.Currently,to solve the problem of excessive Cd,it is essential to research the response mechanisms of L.Chuanxiong to Cd stress.However,there are few reports on its physiological and biochemical responses under Cd stress.In this study,we conducted the hydroponic experiment under 25μM Cd stress,based on the Cd content of the genuine producing areas soil.The results showed that 25μM Cd stress not only had no significant inhibitory effect on the growth of L.Chuanxiong seedlings but also significantly increased the chlorophyll a content(11.79%)and root activity(51.82%)compared with that of the control,which might be a hormesis effect.Further results showed that the absorption and assimilation of NH_(4)^(+)increased in seedlings under 25μM Cd stress,which was associated with high photosynthetic pigments.Here,we initially hypothesized and confirmed that Cd exceedance in the root system of L.Chuanxiong was due to the thickening of the root cell wall,changes in the content of the cell wall components,and chelation of Cd by GSH.There was an increase in cell wall thickness(57.64%)and a significant increase in cellulose(25.48%)content of roots under 25μM Cd stress.In addition,L.Chuanxiong reduced oxidative stress caused by 25μM Cd stress mainly through the GSH/GSSG cycle.Among them,GSH-Px(48.26%)and GR(42.64%)activities were significantly increased,thereby maintaining a high GSH/GSSG ratio.This study preliminarily reveals the response of L.Chuanxiong to Cd stress and the mechanism of Cd enrichment.It provides a theoretical basis for solving the problem of Cd excessive in L.Chuanxiong.展开更多
基金supported by Natural Science Foundation of Sichuan Province(2024NSFSC1832)The National Natural Science Foundation of China(grant numbers 82404796,82373986)+2 种基金China Postdoctoral Science Foundation(grant number 2023M730381)Key R&D Projects of Sichuan Provincial Department of Science and Technology(grant numbers 22ZDYF1861)Natural Science Foundation of Sichuan Province(2024NSFSC1831).
文摘Ligusticum sinense cv.Chuanxiong(L.Chuanxiong),one of the widely used traditional Chinese medicines(TCM),is currently facing the problem of excessive cadmium(Cd)content.This problem has significantly affected the quality and safety of L.Chuanxiong and become a vital factor restricting its clinical application and international trade development.Currently,to solve the problem of excessive Cd,it is essential to research the response mechanisms of L.Chuanxiong to Cd stress.However,there are few reports on its physiological and biochemical responses under Cd stress.In this study,we conducted the hydroponic experiment under 25μM Cd stress,based on the Cd content of the genuine producing areas soil.The results showed that 25μM Cd stress not only had no significant inhibitory effect on the growth of L.Chuanxiong seedlings but also significantly increased the chlorophyll a content(11.79%)and root activity(51.82%)compared with that of the control,which might be a hormesis effect.Further results showed that the absorption and assimilation of NH_(4)^(+)increased in seedlings under 25μM Cd stress,which was associated with high photosynthetic pigments.Here,we initially hypothesized and confirmed that Cd exceedance in the root system of L.Chuanxiong was due to the thickening of the root cell wall,changes in the content of the cell wall components,and chelation of Cd by GSH.There was an increase in cell wall thickness(57.64%)and a significant increase in cellulose(25.48%)content of roots under 25μM Cd stress.In addition,L.Chuanxiong reduced oxidative stress caused by 25μM Cd stress mainly through the GSH/GSSG cycle.Among them,GSH-Px(48.26%)and GR(42.64%)activities were significantly increased,thereby maintaining a high GSH/GSSG ratio.This study preliminarily reveals the response of L.Chuanxiong to Cd stress and the mechanism of Cd enrichment.It provides a theoretical basis for solving the problem of Cd excessive in L.Chuanxiong.
