We present a threedimensional(3D)isotropic imaging of mouse brain using light-sheet fuo-rescent microscopy(LSFM)in conjumction with a multi-view imaging computation.Unlike common single view LSFM is used for mouse bra...We present a threedimensional(3D)isotropic imaging of mouse brain using light-sheet fuo-rescent microscopy(LSFM)in conjumction with a multi-view imaging computation.Unlike common single view LSFM is used for mouse brain imaging,the brain tissue is 3D imaged under eight views in our study,by a home-built selective plane ilumination microscopy(SPIM).An output image containing complete structural infornation as well as significantly improved res olution(~4 times)are then computed based on these eight views of data,using a bead-guided multi-view registration and deconvolution.With superior imaging quality,the astrocyte and pyrarmidal neurons together with their subcellular nerve fbers can be clearly visualized and segmented.With further incuding other computational methods,this study can be potentially scaled up to map the conectome of whole mouse brain with a simple light.sheet microscope.展开更多
The design of optical instruments is an active subject due to improvement in lens techniques, fabrication technology, and data handling capacity. Much remains to do to expand its application to phytopathology, which w...The design of optical instruments is an active subject due to improvement in lens techniques, fabrication technology, and data handling capacity. Much remains to do to expand its application to phytopathology, which would be in particular quite useful to improve crop growth monitoring in countries like Mali. An optical multimodal system for plant samples has been developed to improve the characterization of leaf disease symptoms, provide information on their effects, and avoid their spread. Potentially inexpensive components (laser, lens, turntables camera and sample, filter, lens, camera and computer) have been selected, assembled and aligned on an optical table into a multimodal system operating in transmission, reflection, diffusion and fluorescence. The illumination and observation angles can be adjusted to optimize viewing conditions in the four modes. This scientific contribution has been an initiation into the design and implementation of an optical instrument. Initial results are shown and will now be extended in cooperation with agronomic laboratories in African countries for tests on specific plant diseases in relation with prevailing climate conditions.展开更多
Time-resolved volumetric fluorescence imaging over an extended duration with high spatial/temporal resolution is a key driving force in biomedical research for investigating spatial-temporal dynamics at organism-level...Time-resolved volumetric fluorescence imaging over an extended duration with high spatial/temporal resolution is a key driving force in biomedical research for investigating spatial-temporal dynamics at organism-level systems,yet it remains a major challenge due to the trade-off among imaging speed,light exposure,illumination power,and image quality.Here,we present a deep-learning enhanced light sheet fluorescence microscopy(LSFM)approach that addresses the restoration of rapid volumetric time-lapse imaging with less than 0.03%light exposure and 3.3%acquisition time compared to a typical standard acquisition.We demonstrate that the convolutional neural network(CNN)-transformer network developed here,namely U-net integrated transformer(UI-Trans),successfully achieves the mitigation of complex noise-scattering-coupled degradation and outperforms state-of-the-art deep learning networks,due to its capability of faithfully learning fine details while comprehending complex global features.With the fast generation of appropriate training data via flexible switching between confocal line-scanning LSFM(LS-LSFM)and conventional LSFM,this method achieves a three-to five-fold signal-to-noise ratio(SNR)improvement and~1.8 times contrast improvement in ex vivo zebrafish heart imaging and long-term in vivo 4D(3D morphology+time)imaging of heartbeat dynamics at different developmental stages with ultra-economical acquisitions in terms of light dosage and acquisition time.展开更多
This study proposes a three-dimensional (3D) particle image velocimetry (PIV) method using W- shaped light sheet and color PIV with a digital SLR camera. The uncertainty of the velocity measurement was also studied an...This study proposes a three-dimensional (3D) particle image velocimetry (PIV) method using W- shaped light sheet and color PIV with a digital SLR camera. The uncertainty of the velocity measurement was also studied and it was acceptable. The spatial resolution of the z-direction has much room for improvement by increasing the number of cameras. When applied to the velocity distribution measurement of a thermal vertical buoyant plume, the proposed 3D PIV method is found to be very effective for studying thermal structures and well suited for measuring the airflow velocity field.展开更多
Microcirculation imaging is crucial in understanding the function and health of various tissues and organs.However,conventional imaging methods suffer from fluorescence label dependency,lack of depth resolution,and qu...Microcirculation imaging is crucial in understanding the function and health of various tissues and organs.However,conventional imaging methods suffer from fluorescence label dependency,lack of depth resolution,and quantification inaccuracy.Here,we report a light-sheet dynamic light-scattering imaging(LSHDSI)system to overcome these shortcomings.LSH-DSI utilizes selected plane illumination for an optical sectioning,while a time-frequency analysis method retrieves blood flow velocity estimates from dynamic changes in the detected light intensity.We have performed imaging experiments with zebrafish embryos to obtain angiographs from the trunk and head regions.The results show that LSH-DSI can capture label-free tomographic images of microvasculature and three-dimensional quantitative maps of local blood flow velocities.展开更多
Spinal motoneurons control muscle fibers contraction and drive all motor behaviors in vertebrates.Although spinal motoneurons share the fundamental role of innervating muscle fibers,they exhibit remarkable diversity t...Spinal motoneurons control muscle fibers contraction and drive all motor behaviors in vertebrates.Although spinal motoneurons share the fundamental role of innervating muscle fibers,they exhibit remarkable diversity that reflects their specific identities.Defining the morphological changes during postnatal development is critical for elucidating this diversity.However,our understanding of the three-dimensional(3D)morphology of spinal motoneurons at these stages remains limited,largely due to the lack of high-throughput imaging tools.Using tiling light sheet microscopy combined with tissue clearing methods,we imaged motoneurons of the lateral and median motor column in the cervical and lumbar cord during postnatal development.By analyzing their soma size,we found that motoneurons innervating the upper limbs differentiate into two subpopulations with distinct soma size by postnatal day 14(P14),while differentiation of motoneurons innervating the lower limbs is delayed.Furthermore,coupling adenovirus labeling with 3D volumetric reconstruction,we traced and measured the number and lengths of dendrites of flexor and extensor motoneurons in the lumbar cord,finding that the number of dendrites initially increases and subsequently declines as dendritic order rises.Together,these findings provide a quantitative analysis of the 3D morphological changes underlying spinal motoneuron diversity.展开更多
Bronchus-associated lymphoid tissue(BALT)develops at unpredictable locations around lung bronchi following pulmonary inflammation.The formation and composition of BALT have primarily been investigated by immunohistolo...Bronchus-associated lymphoid tissue(BALT)develops at unpredictable locations around lung bronchi following pulmonary inflammation.The formation and composition of BALT have primarily been investigated by immunohistology that,due to the size of the invested organ,is usually restricted to a limited number of histological sections.To assess the entire BALT of the lung,other approaches are urgently needed.Here,we introduce a novel light sheet microscopy-based approach for assessing lymphoid tissue in the lung.Using antibody staining of whole lung lobes and optical clearing by organic solvents,we present a method that allows in-depth visualization of the entire bronchial tree,the lymphatic vasculature and the immune cell composition of the induced BALT.Furthermore,three-dimensional analysis of the entire lung allows the qualitative and quantitative enumeration of the induced BALT.Using this approach,we show that a single intranasal application of the replication-deficient poxvirus MVA induces BALT that constitutes up to 8%of the entire lung volume in mice deficient in CCR7,in contrast to wild type mice(WT).Furthermore,BALT induced by heat-inactivated E.coli is dominated by a pronounced T cell infiltration in Cxcr5-deficient mice,in contrast to WT mice.展开更多
ZnO as a semiconductor photocatalyst is widely applied in the photodegradation of organic pollutants. Its photocatalytic activity is greatly decreased because of the recombination of photoexcited electrons and holes i...ZnO as a semiconductor photocatalyst is widely applied in the photodegradation of organic pollutants. Its photocatalytic activity is greatly decreased because of the recombination of photoexcited electrons and holes in the bulk. In this work, ZnO sheets are synthesized by adjusting the NaOH concentration under light irradiation at room temperature. Compared with ZnO particles, the ZnO sheets prepared with a light-assisted growth method exhibit a higher rate of photodegradation of methylene blue under UV visible light irradiation. The improved photodegradation rate is mainly attributed to the shortened transport distance of photoexcited electrons, the high surface area, and the surface atom structure modified by the light-assisted growth process. (C) 2016 Science Press and Dalian Institute of Chemical Physics, Chinese Academy of Sciences. Published by Elsevier B.V. and Science Press. All rights reserved.展开更多
The dependence of the performance of organic light-emitting devices（OLEDs） on the sheet resistance of indiumtin-oxide（ITO） anodes was investigated by measuring the steady state current density brightness voltage c...The dependence of the performance of organic light-emitting devices（OLEDs） on the sheet resistance of indiumtin-oxide（ITO） anodes was investigated by measuring the steady state current density brightness voltage characteristics and the electroluminescent spectra. The device with a higher sheet resistance anode shows a lower current density, a lower brightness level, and a higher operation voltage. The electroluminescence（EL） efficiencies of the devices with the same structure but different ITO anodes show more complicated differences. Furthermore, the shift of the light-emitting zone toward the anode was found when an anode with a higher sheet resistance was used. These performance differences are discussed and attributed to the reduction of hole injection and the increase in voltage drop over ITO anode with the increase in sheet resistance.展开更多
The assembly of metal ions Zn2＋, Co2＋ and H2E2Ge2O3 ligand under mild hydro- thermal conditions leads to a mixed 3D transition zinc-cobalt-organogermanate framework, Zn0.88Co0.12（E2Ge2O3） （1, E = -CH2CH2COO-）. T...The assembly of metal ions Zn2＋, Co2＋ and H2E2Ge2O3 ligand under mild hydro- thermal conditions leads to a mixed 3D transition zinc-cobalt-organogermanate framework, Zn0.88Co0.12（E2Ge2O3） （1, E = -CH2CH2COO-）. The structure was determined by single-crystal X-ray diffraction and further characterized by IR spectroscopy, elemental analysis, energy- dispersive X-ray analysis, inductively coupled plasma, powder X-ray diffraction, thermogra- vimetric analysis and fluorescence spectroscopy. Compound 1 crystallizes in the chiral space group P212121, with a = 4.9096（5）, b = 8.9031（11）, c = 24.046（3） A, V = 1051.0（2） A3, C6H8Ge2O7Zn0.88Co0.12, Mr = 401.88, Z = 4, Dc = 2.540 g.cm-1,μ = 7.875 mm-1, F（000） = 775, GOF= 1.016, the final R = 0.0324 and wR = 0.0642. The structure ofl is built up by Ge-O sheets with Ge6 rings and Zn/Co-carboxyl layers. It should be mentioned that although the whole E2Ge2O32- ligand exhibits only one kind of coordination mode, it plays two roles in forming the structure： SBU and linker. In this situation, the E group of H2E2Ge2O3 ligand becomes a determining factor for structural dimensionality. In addition, it displays blue-violet emission light and shows high thermostability.展开更多
基金funding support from 1000 Youth Talents Plan of China (P.F.)Fundamental Research Program of Shenzhen (P.F.,JCYJ20160429182424047)+1 种基金National Science Foundation of China (NSFC31571002,D.Z)Graduates'Innovation Fund of Huazhong University of Science and Technology (5003182004).
文摘We present a threedimensional(3D)isotropic imaging of mouse brain using light-sheet fuo-rescent microscopy(LSFM)in conjumction with a multi-view imaging computation.Unlike common single view LSFM is used for mouse brain imaging,the brain tissue is 3D imaged under eight views in our study,by a home-built selective plane ilumination microscopy(SPIM).An output image containing complete structural infornation as well as significantly improved res olution(~4 times)are then computed based on these eight views of data,using a bead-guided multi-view registration and deconvolution.With superior imaging quality,the astrocyte and pyrarmidal neurons together with their subcellular nerve fbers can be clearly visualized and segmented.With further incuding other computational methods,this study can be potentially scaled up to map the conectome of whole mouse brain with a simple light.sheet microscope.
文摘The design of optical instruments is an active subject due to improvement in lens techniques, fabrication technology, and data handling capacity. Much remains to do to expand its application to phytopathology, which would be in particular quite useful to improve crop growth monitoring in countries like Mali. An optical multimodal system for plant samples has been developed to improve the characterization of leaf disease symptoms, provide information on their effects, and avoid their spread. Potentially inexpensive components (laser, lens, turntables camera and sample, filter, lens, camera and computer) have been selected, assembled and aligned on an optical table into a multimodal system operating in transmission, reflection, diffusion and fluorescence. The illumination and observation angles can be adjusted to optimize viewing conditions in the four modes. This scientific contribution has been an initiation into the design and implementation of an optical instrument. Initial results are shown and will now be extended in cooperation with agronomic laboratories in African countries for tests on specific plant diseases in relation with prevailing climate conditions.
基金supported by National Natural Science Foundation of China(52122008,52270008,52370003,62025502)Guangdong Introducing Innovative and Entrepreneurial Teams of“The Pearl River Talent Recruitment Program”(2021ZT09X044)Shenzhen Technology University under Grant JSZZ202301010.
文摘Time-resolved volumetric fluorescence imaging over an extended duration with high spatial/temporal resolution is a key driving force in biomedical research for investigating spatial-temporal dynamics at organism-level systems,yet it remains a major challenge due to the trade-off among imaging speed,light exposure,illumination power,and image quality.Here,we present a deep-learning enhanced light sheet fluorescence microscopy(LSFM)approach that addresses the restoration of rapid volumetric time-lapse imaging with less than 0.03%light exposure and 3.3%acquisition time compared to a typical standard acquisition.We demonstrate that the convolutional neural network(CNN)-transformer network developed here,namely U-net integrated transformer(UI-Trans),successfully achieves the mitigation of complex noise-scattering-coupled degradation and outperforms state-of-the-art deep learning networks,due to its capability of faithfully learning fine details while comprehending complex global features.With the fast generation of appropriate training data via flexible switching between confocal line-scanning LSFM(LS-LSFM)and conventional LSFM,this method achieves a three-to five-fold signal-to-noise ratio(SNR)improvement and~1.8 times contrast improvement in ex vivo zebrafish heart imaging and long-term in vivo 4D(3D morphology+time)imaging of heartbeat dynamics at different developmental stages with ultra-economical acquisitions in terms of light dosage and acquisition time.
文摘This study proposes a three-dimensional (3D) particle image velocimetry (PIV) method using W- shaped light sheet and color PIV with a digital SLR camera. The uncertainty of the velocity measurement was also studied and it was acceptable. The spatial resolution of the z-direction has much room for improvement by increasing the number of cameras. When applied to the velocity distribution measurement of a thermal vertical buoyant plume, the proposed 3D PIV method is found to be very effective for studying thermal structures and well suited for measuring the airflow velocity field.
基金supported by the following funding sources:Ministry of Education-Singapore MOE2019-T2-2-094 Ministry of Education-Singapore Tier I R-397-000-327-114 ScienceTechnology Project of Jiangsu Province(Grant No.BZ2022056)Biomedical and Health Technology Platform,National University of Singapore(Suzhou)Research Institute.
文摘Microcirculation imaging is crucial in understanding the function and health of various tissues and organs.However,conventional imaging methods suffer from fluorescence label dependency,lack of depth resolution,and quantification inaccuracy.Here,we report a light-sheet dynamic light-scattering imaging(LSHDSI)system to overcome these shortcomings.LSH-DSI utilizes selected plane illumination for an optical sectioning,while a time-frequency analysis method retrieves blood flow velocity estimates from dynamic changes in the detected light intensity.We have performed imaging experiments with zebrafish embryos to obtain angiographs from the trunk and head regions.The results show that LSH-DSI can capture label-free tomographic images of microvasculature and three-dimensional quantitative maps of local blood flow velocities.
基金supported by Westlake Education Foundation,the National Natural Science Foundation of China(32150015)the Zhejiang Province Natural Science Foundation(LR20 C070002).
文摘Spinal motoneurons control muscle fibers contraction and drive all motor behaviors in vertebrates.Although spinal motoneurons share the fundamental role of innervating muscle fibers,they exhibit remarkable diversity that reflects their specific identities.Defining the morphological changes during postnatal development is critical for elucidating this diversity.However,our understanding of the three-dimensional(3D)morphology of spinal motoneurons at these stages remains limited,largely due to the lack of high-throughput imaging tools.Using tiling light sheet microscopy combined with tissue clearing methods,we imaged motoneurons of the lateral and median motor column in the cervical and lumbar cord during postnatal development.By analyzing their soma size,we found that motoneurons innervating the upper limbs differentiate into two subpopulations with distinct soma size by postnatal day 14(P14),while differentiation of motoneurons innervating the lower limbs is delayed.Furthermore,coupling adenovirus labeling with 3D volumetric reconstruction,we traced and measured the number and lengths of dendrites of flexor and extensor motoneurons in the lumbar cord,finding that the number of dendrites initially increases and subsequently declines as dendritic order rises.Together,these findings provide a quantitative analysis of the 3D morphological changes underlying spinal motoneuron diversity.
基金This work was supported by a Lichtenberg-stipend to DTM through the University of Veterinary Medicine,Hannover,the German Excellence Initiative grant EXC62-Rebirth(to RF)DFG grant SFB 900/B1(to RF).
文摘Bronchus-associated lymphoid tissue(BALT)develops at unpredictable locations around lung bronchi following pulmonary inflammation.The formation and composition of BALT have primarily been investigated by immunohistology that,due to the size of the invested organ,is usually restricted to a limited number of histological sections.To assess the entire BALT of the lung,other approaches are urgently needed.Here,we introduce a novel light sheet microscopy-based approach for assessing lymphoid tissue in the lung.Using antibody staining of whole lung lobes and optical clearing by organic solvents,we present a method that allows in-depth visualization of the entire bronchial tree,the lymphatic vasculature and the immune cell composition of the induced BALT.Furthermore,three-dimensional analysis of the entire lung allows the qualitative and quantitative enumeration of the induced BALT.Using this approach,we show that a single intranasal application of the replication-deficient poxvirus MVA induces BALT that constitutes up to 8%of the entire lung volume in mice deficient in CCR7,in contrast to wild type mice(WT).Furthermore,BALT induced by heat-inactivated E.coli is dominated by a pronounced T cell infiltration in Cxcr5-deficient mice,in contrast to WT mice.
基金support from the Institute of Metal Research,Chinese Academy of Sciencessupported by the National Science Fund of China(51202105,21366020)+1 种基金the Natural Science Foundation of Jiangxi Province(20151BAB216006)the Science and Technology Project of Education Department of Jiangxi Province(GJJ14109)
文摘ZnO as a semiconductor photocatalyst is widely applied in the photodegradation of organic pollutants. Its photocatalytic activity is greatly decreased because of the recombination of photoexcited electrons and holes in the bulk. In this work, ZnO sheets are synthesized by adjusting the NaOH concentration under light irradiation at room temperature. Compared with ZnO particles, the ZnO sheets prepared with a light-assisted growth method exhibit a higher rate of photodegradation of methylene blue under UV visible light irradiation. The improved photodegradation rate is mainly attributed to the shortened transport distance of photoexcited electrons, the high surface area, and the surface atom structure modified by the light-assisted growth process. (C) 2016 Science Press and Dalian Institute of Chemical Physics, Chinese Academy of Sciences. Published by Elsevier B.V. and Science Press. All rights reserved.
基金Supported by the National Natural Science Foundation of China(No. 20372060), the Key National Natural Science Foundationof China(No. 20131010), the Important National Natural Science Foundation of China(No. 20490210), the"863"Program(Nos.2002AA302105 and 2002AA324080) and Foreign Communion &Cooperation of National Natural Science Foundation of China(No.20340420326).
文摘The dependence of the performance of organic light-emitting devices（OLEDs） on the sheet resistance of indiumtin-oxide（ITO） anodes was investigated by measuring the steady state current density brightness voltage characteristics and the electroluminescent spectra. The device with a higher sheet resistance anode shows a lower current density, a lower brightness level, and a higher operation voltage. The electroluminescence（EL） efficiencies of the devices with the same structure but different ITO anodes show more complicated differences. Furthermore, the shift of the light-emitting zone toward the anode was found when an anode with a higher sheet resistance was used. These performance differences are discussed and attributed to the reduction of hole injection and the increase in voltage drop over ITO anode with the increase in sheet resistance.
基金supported by the Natural Science Fund for Young Scholars of Fujian Province(No. 2011J05018)the Fund for Young Scholars from Fujian Agriculture and Forestry University (No. 2011xjj06)
文摘The assembly of metal ions Zn2＋, Co2＋ and H2E2Ge2O3 ligand under mild hydro- thermal conditions leads to a mixed 3D transition zinc-cobalt-organogermanate framework, Zn0.88Co0.12（E2Ge2O3） （1, E = -CH2CH2COO-）. The structure was determined by single-crystal X-ray diffraction and further characterized by IR spectroscopy, elemental analysis, energy- dispersive X-ray analysis, inductively coupled plasma, powder X-ray diffraction, thermogra- vimetric analysis and fluorescence spectroscopy. Compound 1 crystallizes in the chiral space group P212121, with a = 4.9096（5）, b = 8.9031（11）, c = 24.046（3） A, V = 1051.0（2） A3, C6H8Ge2O7Zn0.88Co0.12, Mr = 401.88, Z = 4, Dc = 2.540 g.cm-1,μ = 7.875 mm-1, F（000） = 775, GOF= 1.016, the final R = 0.0324 and wR = 0.0642. The structure ofl is built up by Ge-O sheets with Ge6 rings and Zn/Co-carboxyl layers. It should be mentioned that although the whole E2Ge2O32- ligand exhibits only one kind of coordination mode, it plays two roles in forming the structure： SBU and linker. In this situation, the E group of H2E2Ge2O3 ligand becomes a determining factor for structural dimensionality. In addition, it displays blue-violet emission light and shows high thermostability.
