The chloroplast DNA （cpDNA） and mitochondrial DNA （mtDNA） of 16 Populus species （Section Leuce） and their F1 generation were detected using PCR-RFLP technique. The results show that cpDNA in the F1 generation of...The chloroplast DNA （cpDNA） and mitochondrial DNA （mtDNA） of 16 Populus species （Section Leuce） and their F1 generation were detected using PCR-RFLP technique. The results show that cpDNA in the F1 generation of 22 hybrid combinations was inherited maternally, which supported the conclusions of the study of plasmid cytology. The mtDNA fragments amplified by PCR were consistent with the restriction maps in all hybrid combinations and no polymorphism was detected, indicating that the Section Leuce is highly conserved in mitochondrial gene sequences. These results provided direct evidence of maternal chloroplast inheritance in Populus tomentosa, P. bolleana, P. davidiana, P. adenopoda, P. tomentosa × P. bolleana, P. alba × P. glandulosa and P. alba × P. tomentosa.展开更多
基金the National Science Foundation of China (Grant Nos. 30771747 and 30640036)the Beijing Natural Science Foundation (No. 6042020)+1 种基金the research projects supported by the Hebei Education Department (No. Z2010102)the Baoding University (No. 2010Z02)
文摘The chloroplast DNA （cpDNA） and mitochondrial DNA （mtDNA） of 16 Populus species （Section Leuce） and their F1 generation were detected using PCR-RFLP technique. The results show that cpDNA in the F1 generation of 22 hybrid combinations was inherited maternally, which supported the conclusions of the study of plasmid cytology. The mtDNA fragments amplified by PCR were consistent with the restriction maps in all hybrid combinations and no polymorphism was detected, indicating that the Section Leuce is highly conserved in mitochondrial gene sequences. These results provided direct evidence of maternal chloroplast inheritance in Populus tomentosa, P. bolleana, P. davidiana, P. adenopoda, P. tomentosa × P. bolleana, P. alba × P. glandulosa and P. alba × P. tomentosa.
