Background: An experiment was conducted to evaluate the effects of feed restriction(FR) and sex on the quantitative and qualitative carcass traits of Morada Nova lambs. Thirty-five animals with an initial body weight ...Background: An experiment was conducted to evaluate the effects of feed restriction(FR) and sex on the quantitative and qualitative carcass traits of Morada Nova lambs. Thirty-five animals with an initial body weight of 14.5 ± 0.89 kg and age of 120 d were used in a completely randomized study with a 3 × 3 factorial scheme consisting of three sexes(11 entire males, 12 castrated males and 12 females) and three levels of feeding(ad libitum – AL and 30% and 60% FR).Results: Entire males presented greater hot and cold carcass weights(P < 0.05), followed by castrated males and females. However, the hot carcass yield was higher for females and castrated males than for entire males. Luminosity values were influenced(P < 0.05) by sex, with entire males presenting higher values than castrated males and females.Females showed higher(P < 0.05) concentrations of linoleic acid and arachidonic acid in the meat of the longissimus thoracis muscle. The meat of animals submitted to AL intake and 30% FR showed similar(P > 0.05) concentrations, and the concentrations of palmitic acid, palmitoleic acid, stearic acid, oleic acid and conjugated linoleic acid were higher(P < 0.05) than those of animals with 60% FR. The meat of females had a higher ω6/ω3 ratio and lower h/H ratio, and females had greater levels of feeding. The meat of animals on the 60% FR diet had a greater ω6/ω3 ratio, lower h/H ratio and lower concentration of desirable fatty acids in addition to a greater atherogenicity index(AI) and thrombogenicity index(TI).Conclusion: Lambs of different sexes had carcasses with different quantitative traits without total influence on the chemical and physical meat characteristics. The lipid profile of the meat was less favorable to consumer health when the animals were female or submitted to 60% feed restriction.展开更多
旨在研究中国本土槐猪和引入瘦肉型猪(杜洛克猪、大约克夏猪、长白猪)在生长育肥相关基因MC4R和MSTN、脊椎数量相关基因NR6A1和VRTN、肉质性状相关基因PHKG1和PRKAG3以及病原感染相关基因SYNGR2和FUT1这8个基因9个SNP位点的遗传变异特...旨在研究中国本土槐猪和引入瘦肉型猪(杜洛克猪、大约克夏猪、长白猪)在生长育肥相关基因MC4R和MSTN、脊椎数量相关基因NR6A1和VRTN、肉质性状相关基因PHKG1和PRKAG3以及病原感染相关基因SYNGR2和FUT1这8个基因9个SNP位点的遗传变异特征。利用竞争性等位基因特异性PCR(KASP)和聚合酶链反应-限制性片段长度多态性(PCR-restriction fragment length polymorphism,PCR-RFLP)方法对突变位点进行基因分型,根据原始文献对各位点进行有利等位基因判定,并制作各猪种在各位点有利等位基因和不利等位基因的百分比示意图。结果:槐猪在NR6A1基因突变位点存在一定多态性,有利等位基因频率为0.24,其他位点表现为单一基因型,其中,PHKG1、PRKAG3突变位点为有利等位基因,而在MC4R、MSTN、VRTN、SYNGR2和FUT1突变位点为不利等位基因;杜洛克猪在MC4R、NR6A1、VRTN、SYNGR2和FUT1突变位点有利等位基因占优,然而,PHKG1基因不利等位基因仍有一定的比例;大约克夏猪在MC4R、SYNGR2、NR6A1和VRTN基因突变位点有利等位基因占优,而其他位点为不利等位基因;长白猪在MC4R、MSTN、PRKAG3(p.Val249Ile)和SYNGR2这4个基因突变位点不利基因占优;杜洛克猪在VRTN基因、长白猪在MC4R基因突变位点处于遗传不平衡状态(P<0.05)。以上结果为槐猪品种保护与利用以及瘦肉型猪种分子遗传标记选育提供了有益的信息。展开更多
建立了超高效液相色谱-串联质谱快速简单地同时测定猪尿液中30种不同种类"瘦肉精"药物(赛庚啶、可乐定及28种β-受体激动剂类)残留的方法。对液相色谱分离条件、MS/MS检测参数及样品前处理方式进行了优化。试样经5000 r/min离...建立了超高效液相色谱-串联质谱快速简单地同时测定猪尿液中30种不同种类"瘦肉精"药物(赛庚啶、可乐定及28种β-受体激动剂类)残留的方法。对液相色谱分离条件、MS/MS检测参数及样品前处理方式进行了优化。试样经5000 r/min离心5 min后直接经MCX柱净化,分别用3 m L水和3 m L甲醇淋洗,5%氨化甲醇进行洗脱,N2吹干后以流动相进行复溶,UPLC-MS/MS进行测定。结果表明,30种药物可在5.0 min内有效分离;各药物在0.1~10μg/L范围内呈良好的线性关系,相关系数均大于0.992;方法的检出限为0.1μg/L,定量限为0.3μg/L。在3个浓度水平下的平均回收率为67.6%~103.2%,日内、日间相对标准偏差分别为2.8%~16.8%和2.6%~15.8%。展开更多
基金supported by the National Council for Scientific and Technological Development(CNPq-Brazil)
文摘Background: An experiment was conducted to evaluate the effects of feed restriction(FR) and sex on the quantitative and qualitative carcass traits of Morada Nova lambs. Thirty-five animals with an initial body weight of 14.5 ± 0.89 kg and age of 120 d were used in a completely randomized study with a 3 × 3 factorial scheme consisting of three sexes(11 entire males, 12 castrated males and 12 females) and three levels of feeding(ad libitum – AL and 30% and 60% FR).Results: Entire males presented greater hot and cold carcass weights(P < 0.05), followed by castrated males and females. However, the hot carcass yield was higher for females and castrated males than for entire males. Luminosity values were influenced(P < 0.05) by sex, with entire males presenting higher values than castrated males and females.Females showed higher(P < 0.05) concentrations of linoleic acid and arachidonic acid in the meat of the longissimus thoracis muscle. The meat of animals submitted to AL intake and 30% FR showed similar(P > 0.05) concentrations, and the concentrations of palmitic acid, palmitoleic acid, stearic acid, oleic acid and conjugated linoleic acid were higher(P < 0.05) than those of animals with 60% FR. The meat of females had a higher ω6/ω3 ratio and lower h/H ratio, and females had greater levels of feeding. The meat of animals on the 60% FR diet had a greater ω6/ω3 ratio, lower h/H ratio and lower concentration of desirable fatty acids in addition to a greater atherogenicity index(AI) and thrombogenicity index(TI).Conclusion: Lambs of different sexes had carcasses with different quantitative traits without total influence on the chemical and physical meat characteristics. The lipid profile of the meat was less favorable to consumer health when the animals were female or submitted to 60% feed restriction.
文摘旨在研究中国本土槐猪和引入瘦肉型猪(杜洛克猪、大约克夏猪、长白猪)在生长育肥相关基因MC4R和MSTN、脊椎数量相关基因NR6A1和VRTN、肉质性状相关基因PHKG1和PRKAG3以及病原感染相关基因SYNGR2和FUT1这8个基因9个SNP位点的遗传变异特征。利用竞争性等位基因特异性PCR(KASP)和聚合酶链反应-限制性片段长度多态性(PCR-restriction fragment length polymorphism,PCR-RFLP)方法对突变位点进行基因分型,根据原始文献对各位点进行有利等位基因判定,并制作各猪种在各位点有利等位基因和不利等位基因的百分比示意图。结果:槐猪在NR6A1基因突变位点存在一定多态性,有利等位基因频率为0.24,其他位点表现为单一基因型,其中,PHKG1、PRKAG3突变位点为有利等位基因,而在MC4R、MSTN、VRTN、SYNGR2和FUT1突变位点为不利等位基因;杜洛克猪在MC4R、NR6A1、VRTN、SYNGR2和FUT1突变位点有利等位基因占优,然而,PHKG1基因不利等位基因仍有一定的比例;大约克夏猪在MC4R、SYNGR2、NR6A1和VRTN基因突变位点有利等位基因占优,而其他位点为不利等位基因;长白猪在MC4R、MSTN、PRKAG3(p.Val249Ile)和SYNGR2这4个基因突变位点不利基因占优;杜洛克猪在VRTN基因、长白猪在MC4R基因突变位点处于遗传不平衡状态(P<0.05)。以上结果为槐猪品种保护与利用以及瘦肉型猪种分子遗传标记选育提供了有益的信息。
文摘建立了超高效液相色谱-串联质谱快速简单地同时测定猪尿液中30种不同种类"瘦肉精"药物(赛庚啶、可乐定及28种β-受体激动剂类)残留的方法。对液相色谱分离条件、MS/MS检测参数及样品前处理方式进行了优化。试样经5000 r/min离心5 min后直接经MCX柱净化,分别用3 m L水和3 m L甲醇淋洗,5%氨化甲醇进行洗脱,N2吹干后以流动相进行复溶,UPLC-MS/MS进行测定。结果表明,30种药物可在5.0 min内有效分离;各药物在0.1~10μg/L范围内呈良好的线性关系,相关系数均大于0.992;方法的检出限为0.1μg/L,定量限为0.3μg/L。在3个浓度水平下的平均回收率为67.6%~103.2%,日内、日间相对标准偏差分别为2.8%~16.8%和2.6%~15.8%。
