Objective: The purpose of this study was to evaluate health education using videos and leaflets for preconception care (PCC) awareness among adolescent females up to six months after the health education. Methods: The...Objective: The purpose of this study was to evaluate health education using videos and leaflets for preconception care (PCC) awareness among adolescent females up to six months after the health education. Methods: The subjects were female university students living in the Kinki area. A longitudinal survey was conducted on 67 members in the intervention group, who received the health education, and 52 members in the control group, who did not receive the health education. The primary outcome measures were knowledge of PCC and the subscales of the Health Promotion Lifestyle Profile. Surveys were conducted before, after, and six months after the intervention in the intervention group, and an initial survey and survey six months later were conducted in the control group. Cochran’s Q test, Bonferroni’s multiple comparison test, and McNemar’s test were used to analyze the knowledge of PCC data. The Health Awareness, Nutrition, and Stress Management subscales of the Health Promotion Lifestyle Profile were analyzed by paired t-test, and comparisons between the intervention and control groups were performed using the two-way repeated measures analysis of variance. Results: In the intervention group of 67 people, the number of subjects who answered “correct” for five of the nine items concerning knowledge of PCC increased immediately after the health education (P = 0.006) but decreased for five items from immediately after the health education to six months later (P = 0.043). In addition, the number of respondents who answered “correct” for “low birth weight infants and future lifestyle-related diseases” (P = 0.016) increased after six months compared with before the health education. For the 52 subjects in the control group, there was no change in the number of subjects who answered “correct” for eight out of the nine items after six months. There was also no increase in scores for the Health Promotion Lifestyle Profile after six months for either the intervention or control group. Conclusion: Providing health education about PCC using videos and leaflets to adolescent females was shown to enhance the knowledge of PCC immediately after the education.展开更多
The Agrobacterium-mediated transient expression system with conventional binary vectors is well established in tobacco leaves,while the same system applied to tomato leaflets has relatively low expression efficiency.H...The Agrobacterium-mediated transient expression system with conventional binary vectors is well established in tobacco leaves,while the same system applied to tomato leaflets has relatively low expression efficiency.However,impacts of the leaf age,inoculation method and incubation condition after Agrobacterium infiltration on transient protein expression efficiency are seldom investigated.In this study,we optimize Agrobacterium-mediated transient expression system using conventional binary vectors to achieve the high efficiency of target gene expression in tomato leaflets.We transiently express GFP and a nucleus-localized gene SlUVI4 fused with GFP in detached 10-,20-,and 30-day-old leaflets.The cutting points of leaflets are embedded in MS medium after the Agrobacterium-mediated vacuum infiltration,and all leaflets are kept in the dark before use.The 10-and 30-day-old leaflets have more damage than 20-day-old leaflets after the infiltration.展开更多
A chemically prepared carbon was synthesized from date palm leaflets via sulphuric acid carbonization at 160℃. Adsorption of ciprofloxacin （CIP） from aqueous solution was investigated in terms of time, pH, concentr...A chemically prepared carbon was synthesized from date palm leaflets via sulphuric acid carbonization at 160℃. Adsorption of ciprofloxacin （CIP） from aqueous solution was investigated in terms of time, pH, concentration, temperature and adsorbent status （wet and dry）. The equilibrium time was found to be 48 hr. The adsorption rate was enhanced by raising the temperature for both adsorbents, with adsorption data fitting a pseudo second-order model well. The activation energy, Ea, was found to be 17 kJ/mol, indicating a diffusion-controlled, physical adsorption process. The maximum adsorption was found at initial pH 6. The wet adsorbent showed faster removal with higher uptake than the dry adsorbent, with increased performance as temperature increased （25--45℃）. The equilibrium data were found to fit the Langmuir model better than the Freundlich model. The thermodynamic parameters showed that the adsorption process is spontaneous and endothermic. The adsorption mechanism is mainly related to cation exchange and hydrogen bonding.展开更多
The mechanisms of systolic anterior motion(SAM)of the mitral valve in hypertrophic obstructive cardiomyopathy(HOCM)remain unclear.To investigate the angle of attack between blood flow and mitral valve leaflets at pre-...The mechanisms of systolic anterior motion(SAM)of the mitral valve in hypertrophic obstructive cardiomyopathy(HOCM)remain unclear.To investigate the angle of attack between blood flow and mitral valve leaflets at pre-SAM time point,patient-specific CT-based computational models were constructed for 5 patients receiving septal myectomy surgery to obtain pre-and post-operative 2D vector flow mapping.The comparisons between pre-and post-operative angles of attack based on 2D vector flow mapping of 5 patients were performed.It was found that there was no statistically significant difference between pre-and post-operative angles of attack(61.1±t wa o vs.56.2±56.o,p=0.306,n=5).Therefore,we propose that the angle of attack might not play an important role in the initiation of SAM.展开更多
Health communication is critical component of effective health delivery. While there are studies on the readability Patient Information Leaflets (PILs) around the world, so far, there is only one Ghanaian study that e...Health communication is critical component of effective health delivery. While there are studies on the readability Patient Information Leaflets (PILs) around the world, so far, there is only one Ghanaian study that explored the language of patient information leaflets for malaria. The present study explores the language of PILs of seven common ailments in Ghana. In all, 68 leaflets were purposely sampled for the study. Using SMOG and Flesch Kincaid grade level readability formulae, the researcher measured the readability of PILs and discovered that the readability of all the PILs was difficult to read except the dewormer leaflets that scored standard reading score. Further statistical analysis reveal that the lexical and syntactic complexity of the leaflets were not statistically different. Finally, an interview with 20 patients confirmed that the leaflets are difficult to read due to the complex words and long sentence structures. It is recommended that the PILs be revised to make the leaflets readable.展开更多
Background Currently used heart valve prostheses are associated with anticoagulation complications or limited durability. The advancement of stem cell study and tissue-engineered heart valve research may offer a relat...Background Currently used heart valve prostheses are associated with anticoagulation complications or limited durability. The advancement of stem cell study and tissue-engineered heart valve research may offer a relatively ideal solution to these problems. Methods Bone marrow was aspirated from sternum of lamb goats to isolate BMCs. Cells were identified by flow cytometry and its capacity of differentiation. Cellular viability was assessed with Rhdomine 123 staining. 1 × 10^7cells were seeded on a patch of PGA sheet. After two-day in vitro culture, autologous cell/ scaffold sheets were used to replace the right posterior pulmonary valve leaflets under cardiopulmonary bypass. The leaflets were explanted at 2 days, 2, 6, 8 and 10 weeks after implantation. The samples were examined macroscopically, histologically, immunohistochemically, and by Scanning Electron Microscope （SEM）. Two goats were implanted with acellular sheets and established as a control group. Results BMCs exhibited fibroblastoid morphology with good viability. Flow cytometry showed negative CD14 and CD45 expression. In vitro cultured BMCs demonstrated the potential to differentiate into adipocytes. The explanted leaflets resembled the characteristics of native extracellular matrix was leaflets macroscopicaIly in the cellular group. Histology showed synthesized and cells were distributed in the single-layered leaflets.Immunohistochemistry revealed positive staining for yon Willebrand factor, α-SMA, vimentin. A confluent cell surface was formed on the explanted TEHLs. No calcium deposited on the leaflets. In control group, the acellular scaffolds were completely degraded, without leaflet remained at 8 weeks. Conclusions It is possible to create tissue-engineered heart valves in vivo using autologous bone marrow-derived cells.展开更多
Mitral valve(MV)tissue engineering is still in its early stage,and one major challenge in MV tissue engineering is to identify appropriate scaffold materials.With the potential of acellular MV scaffolds being demonstr...Mitral valve(MV)tissue engineering is still in its early stage,and one major challenge in MV tissue engineering is to identify appropriate scaffold materials.With the potential of acellular MV scaffolds being demonstrated recently,it is important to have a full understanding of the biomechanics of the native MV components and their acellular scaffolds.In this study,we have successfully characterized the structural and mechanical properties of porcine MV components,including anterior leaflet(AL),posterior leaflet(PL),strut chordae,and basal chordae,before and after decellularization.Quantitative DNA assay showed more than 90%reduction in DNA content,and Griffonia simplicifolia(GS)lectin immunohistochemistry confirmed the complete lack of porcine𝛼-Gal antigen in the acellular MV components.In the acellular AL and PL,the atrialis,spongiosa,and fibrosa trilayered structure,along with its ECM constitutes,i.e.,collagen fibers,elastin fibers,and portion of GAGs,were preserved.Never-theless,the ECM of both AL and PL experienced a certain degree of disruption,exhibiting a less dense,porous ECM morphology.The overall anatomical morphology of the strut and basal chordae were also maintained af-ter decellularization,with longitudinal morphology experiencing minimum disruption,but the cross-sectional morphology exhibiting evenly-distributed porous structure.In the acellular AL and PL,the nonlinear anisotropic biaxial mechanical behavior was overall preserved;however,uniaxial tensile tests showed that the removal of cellular content and the disruption of structural ECM did result in small decreases in maximum tensile modulus,tissue extensibility,failure stress,and failure strain for both MV leaflets and chordae.展开更多
Mitral regurgitation (MR) is a highly prevalent valvular heart disease globally,with untreated severe cases demonstrating associations with elevated morbidity,mortality,and adverse cardiovascular outcomes.[1,2]While t...Mitral regurgitation (MR) is a highly prevalent valvular heart disease globally,with untreated severe cases demonstrating associations with elevated morbidity,mortality,and adverse cardiovascular outcomes.[1,2]While transcatheter edge-toedge repair (TEER) has emerged as an alternative option for high surgical risk patients with severe MR,[3,4]severe MR of Carpentier class IIIa (characterized by restricted leaflet motion during both systole and diastole) has been considered a relative contraindication for TEER interventions due to stenosis risk and procedural complexity.[5,6]展开更多
Group B Streptococcus(GBS;Streptococcus agalactiae)is a gram-positive coccus that colonizes the gastrointestinal and genital tracts in adults,as well as the upper respiratory tract in infants.While it has been thought...Group B Streptococcus(GBS;Streptococcus agalactiae)is a gram-positive coccus that colonizes the gastrointestinal and genital tracts in adults,as well as the upper respiratory tract in infants.While it has been thought that GBS only results in invasive disease in pregnant females and neonates,recent literature has suggested an increasing incidence of invasive GBS among non-pregnant individuals within the United States.展开更多
On the sunny days, there were little diurnal changes in both azimuth and directional angle for either sun_ or shade_leaflet. However, there existed a significant diurnal change in midrib angle that reflected movements...On the sunny days, there were little diurnal changes in both azimuth and directional angle for either sun_ or shade_leaflet. However, there existed a significant diurnal change in midrib angle that reflected movements regarding evasion of light stress around noon hours. On the cloudy day, a very little diurnal difference was found in azimuth, directional and midrib angle. It is suggested that changing light environment is the main factor for inducing leaflet movement of Robinia pseudoacacia , and the rhythmical movement does not attribute to the leaflet movement. Leaf orientation control test showed that the photosynthetic rate ( Pn ), stomatal conductance ( g s ) and transpiration ( Tr ) of the artificial fixed_leaflets were significantly lower than that of control_leaflets. And the leaf temperature ( Tl ) of the fixed leaflets significantly exceeded that of control_leaflets, which may attribute to the significant difference of light interception between the fixed and control_leaflets. The light_dependent leaflet movement is the morphological adjustment of maintaining optimal physiological status.展开更多
文摘Objective: The purpose of this study was to evaluate health education using videos and leaflets for preconception care (PCC) awareness among adolescent females up to six months after the health education. Methods: The subjects were female university students living in the Kinki area. A longitudinal survey was conducted on 67 members in the intervention group, who received the health education, and 52 members in the control group, who did not receive the health education. The primary outcome measures were knowledge of PCC and the subscales of the Health Promotion Lifestyle Profile. Surveys were conducted before, after, and six months after the intervention in the intervention group, and an initial survey and survey six months later were conducted in the control group. Cochran’s Q test, Bonferroni’s multiple comparison test, and McNemar’s test were used to analyze the knowledge of PCC data. The Health Awareness, Nutrition, and Stress Management subscales of the Health Promotion Lifestyle Profile were analyzed by paired t-test, and comparisons between the intervention and control groups were performed using the two-way repeated measures analysis of variance. Results: In the intervention group of 67 people, the number of subjects who answered “correct” for five of the nine items concerning knowledge of PCC increased immediately after the health education (P = 0.006) but decreased for five items from immediately after the health education to six months later (P = 0.043). In addition, the number of respondents who answered “correct” for “low birth weight infants and future lifestyle-related diseases” (P = 0.016) increased after six months compared with before the health education. For the 52 subjects in the control group, there was no change in the number of subjects who answered “correct” for eight out of the nine items after six months. There was also no increase in scores for the Health Promotion Lifestyle Profile after six months for either the intervention or control group. Conclusion: Providing health education about PCC using videos and leaflets to adolescent females was shown to enhance the knowledge of PCC immediately after the education.
基金support of Taishan Scholar Foundation of Shandong Province(tsqn201909073,tsqn201812034)National Natural Science Foundation of China(31872951)。
文摘The Agrobacterium-mediated transient expression system with conventional binary vectors is well established in tobacco leaves,while the same system applied to tomato leaflets has relatively low expression efficiency.However,impacts of the leaf age,inoculation method and incubation condition after Agrobacterium infiltration on transient protein expression efficiency are seldom investigated.In this study,we optimize Agrobacterium-mediated transient expression system using conventional binary vectors to achieve the high efficiency of target gene expression in tomato leaflets.We transiently express GFP and a nucleus-localized gene SlUVI4 fused with GFP in detached 10-,20-,and 30-day-old leaflets.The cutting points of leaflets are embedded in MS medium after the Agrobacterium-mediated vacuum infiltration,and all leaflets are kept in the dark before use.The 10-and 30-day-old leaflets have more damage than 20-day-old leaflets after the infiltration.
文摘A chemically prepared carbon was synthesized from date palm leaflets via sulphuric acid carbonization at 160℃. Adsorption of ciprofloxacin （CIP） from aqueous solution was investigated in terms of time, pH, concentration, temperature and adsorbent status （wet and dry）. The equilibrium time was found to be 48 hr. The adsorption rate was enhanced by raising the temperature for both adsorbents, with adsorption data fitting a pseudo second-order model well. The activation energy, Ea, was found to be 17 kJ/mol, indicating a diffusion-controlled, physical adsorption process. The maximum adsorption was found at initial pH 6. The wet adsorbent showed faster removal with higher uptake than the dry adsorbent, with increased performance as temperature increased （25--45℃）. The equilibrium data were found to fit the Langmuir model better than the Freundlich model. The thermodynamic parameters showed that the adsorption process is spontaneous and endothermic. The adsorption mechanism is mainly related to cation exchange and hydrogen bonding.
基金The authors would like to acknowledge the research support from Natural Science Foundation of Fujian Province of China(Grant No.2017J01009)Fundamental Research Funds for the Central Universities(Grant No.20720180004)National Heart,Lung and Blood Institute grants R01 HL089269,and National Sciences Foundation of China(Grant No.11672001,81571691).
文摘The mechanisms of systolic anterior motion(SAM)of the mitral valve in hypertrophic obstructive cardiomyopathy(HOCM)remain unclear.To investigate the angle of attack between blood flow and mitral valve leaflets at pre-SAM time point,patient-specific CT-based computational models were constructed for 5 patients receiving septal myectomy surgery to obtain pre-and post-operative 2D vector flow mapping.The comparisons between pre-and post-operative angles of attack based on 2D vector flow mapping of 5 patients were performed.It was found that there was no statistically significant difference between pre-and post-operative angles of attack(61.1±t wa o vs.56.2±56.o,p=0.306,n=5).Therefore,we propose that the angle of attack might not play an important role in the initiation of SAM.
文摘Health communication is critical component of effective health delivery. While there are studies on the readability Patient Information Leaflets (PILs) around the world, so far, there is only one Ghanaian study that explored the language of patient information leaflets for malaria. The present study explores the language of PILs of seven common ailments in Ghana. In all, 68 leaflets were purposely sampled for the study. Using SMOG and Flesch Kincaid grade level readability formulae, the researcher measured the readability of PILs and discovered that the readability of all the PILs was difficult to read except the dewormer leaflets that scored standard reading score. Further statistical analysis reveal that the lexical and syntactic complexity of the leaflets were not statistically different. Finally, an interview with 20 patients confirmed that the leaflets are difficult to read due to the complex words and long sentence structures. It is recommended that the PILs be revised to make the leaflets readable.
基金supported by the grant from Guangdong Nature Science Foundation(7001117)
文摘Background Currently used heart valve prostheses are associated with anticoagulation complications or limited durability. The advancement of stem cell study and tissue-engineered heart valve research may offer a relatively ideal solution to these problems. Methods Bone marrow was aspirated from sternum of lamb goats to isolate BMCs. Cells were identified by flow cytometry and its capacity of differentiation. Cellular viability was assessed with Rhdomine 123 staining. 1 × 10^7cells were seeded on a patch of PGA sheet. After two-day in vitro culture, autologous cell/ scaffold sheets were used to replace the right posterior pulmonary valve leaflets under cardiopulmonary bypass. The leaflets were explanted at 2 days, 2, 6, 8 and 10 weeks after implantation. The samples were examined macroscopically, histologically, immunohistochemically, and by Scanning Electron Microscope （SEM）. Two goats were implanted with acellular sheets and established as a control group. Results BMCs exhibited fibroblastoid morphology with good viability. Flow cytometry showed negative CD14 and CD45 expression. In vitro cultured BMCs demonstrated the potential to differentiate into adipocytes. The explanted leaflets resembled the characteristics of native extracellular matrix was leaflets macroscopicaIly in the cellular group. Histology showed synthesized and cells were distributed in the single-layered leaflets.Immunohistochemistry revealed positive staining for yon Willebrand factor, α-SMA, vimentin. A confluent cell surface was formed on the explanted TEHLs. No calcium deposited on the leaflets. In control group, the acellular scaffolds were completely degraded, without leaflet remained at 8 weeks. Conclusions It is possible to create tissue-engineered heart valves in vivo using autologous bone marrow-derived cells.
基金supported in part by NIH R15HL159599,R15HL140503,and AHA GRNT17150041,GRNT959644.
文摘Mitral valve(MV)tissue engineering is still in its early stage,and one major challenge in MV tissue engineering is to identify appropriate scaffold materials.With the potential of acellular MV scaffolds being demonstrated recently,it is important to have a full understanding of the biomechanics of the native MV components and their acellular scaffolds.In this study,we have successfully characterized the structural and mechanical properties of porcine MV components,including anterior leaflet(AL),posterior leaflet(PL),strut chordae,and basal chordae,before and after decellularization.Quantitative DNA assay showed more than 90%reduction in DNA content,and Griffonia simplicifolia(GS)lectin immunohistochemistry confirmed the complete lack of porcine𝛼-Gal antigen in the acellular MV components.In the acellular AL and PL,the atrialis,spongiosa,and fibrosa trilayered structure,along with its ECM constitutes,i.e.,collagen fibers,elastin fibers,and portion of GAGs,were preserved.Never-theless,the ECM of both AL and PL experienced a certain degree of disruption,exhibiting a less dense,porous ECM morphology.The overall anatomical morphology of the strut and basal chordae were also maintained af-ter decellularization,with longitudinal morphology experiencing minimum disruption,but the cross-sectional morphology exhibiting evenly-distributed porous structure.In the acellular AL and PL,the nonlinear anisotropic biaxial mechanical behavior was overall preserved;however,uniaxial tensile tests showed that the removal of cellular content and the disruption of structural ECM did result in small decreases in maximum tensile modulus,tissue extensibility,failure stress,and failure strain for both MV leaflets and chordae.
基金supported by the 1·3·5 Project for Disciplines of Excellence from West China Hospital of Sichuan University(ZYGD23021&23HXFH009)Sichuan Science and Technology Program(No.2025ZNSFSC1698)the Sichuan Provincial Cadre Health Research Program(ZH2024-103).
文摘Mitral regurgitation (MR) is a highly prevalent valvular heart disease globally,with untreated severe cases demonstrating associations with elevated morbidity,mortality,and adverse cardiovascular outcomes.[1,2]While transcatheter edge-toedge repair (TEER) has emerged as an alternative option for high surgical risk patients with severe MR,[3,4]severe MR of Carpentier class IIIa (characterized by restricted leaflet motion during both systole and diastole) has been considered a relative contraindication for TEER interventions due to stenosis risk and procedural complexity.[5,6]
文摘Group B Streptococcus(GBS;Streptococcus agalactiae)is a gram-positive coccus that colonizes the gastrointestinal and genital tracts in adults,as well as the upper respiratory tract in infants.While it has been thought that GBS only results in invasive disease in pregnant females and neonates,recent literature has suggested an increasing incidence of invasive GBS among non-pregnant individuals within the United States.
文摘On the sunny days, there were little diurnal changes in both azimuth and directional angle for either sun_ or shade_leaflet. However, there existed a significant diurnal change in midrib angle that reflected movements regarding evasion of light stress around noon hours. On the cloudy day, a very little diurnal difference was found in azimuth, directional and midrib angle. It is suggested that changing light environment is the main factor for inducing leaflet movement of Robinia pseudoacacia , and the rhythmical movement does not attribute to the leaflet movement. Leaf orientation control test showed that the photosynthetic rate ( Pn ), stomatal conductance ( g s ) and transpiration ( Tr ) of the artificial fixed_leaflets were significantly lower than that of control_leaflets. And the leaf temperature ( Tl ) of the fixed leaflets significantly exceeded that of control_leaflets, which may attribute to the significant difference of light interception between the fixed and control_leaflets. The light_dependent leaflet movement is the morphological adjustment of maintaining optimal physiological status.
