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First Record of the Blue-banded Sea Krait(Laticauda laticaudata,Reptilia:Squamata:Elapidae:Laticaudinae)on Jeju Island,South Korea
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作者 Jaejin PARK Kyo-Soung KOO +2 位作者 Il-Hun KIM Woo-Jin CHOI Daesik PARK 《Asian Herpetological Research》 SCIE CSCD 2017年第2期131-136,共6页
We report the first recorded capture of a blue-banded sea snake(Laticauda laticaudata Linnaeus, 1758, Jobeuntti Kun Badabam in Korean) in South Korea based on one male specimen collected from Marado-ri, Seogwiposi, ... We report the first recorded capture of a blue-banded sea snake(Laticauda laticaudata Linnaeus, 1758, Jobeuntti Kun Badabam in Korean) in South Korea based on one male specimen collected from Marado-ri, Seogwiposi, Jeju-do on 20 October 2016. The morphological features of the lateral nostrils, the much wider ventrals than adjacent dorsals, the horizontally undivided rostral, the two prefrontals, and the uniform black bands on the body indicate that the specimen is L. laticaudata. An analysis of the partial mitochondrial cytochrome b gene sequence indicated that the specimen fits well into the known L. laticaudata phylogenetic group, which confirms that the sea krait is L. laticaudata. Including this report, five species of sea snakes(L. laticaudata, L. semifasciata, Hydrophis platurus, H. cyanocinctus, and H. melanocephalus) have now been reported in Korean waters. 展开更多
关键词 sea snake Hydrophiinae Laticaudinae blue-banded sea snake laticauda laticaudata
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半环扁尾海蛇神经毒素的融合表达及活性检测 被引量:3
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作者 吴方晖 董玉轩 +4 位作者 应天翼 张华东 张金平 王惠芳 李艳军 《生物技术通讯》 CAS 2006年第5期713-715,共3页
目的:将海蛇神经毒素基因克隆到融合表达载体pET32a中,诱导表达海蛇神经毒素,鉴定融合表达蛋白的生物功能,为大量合成海蛇神经毒素奠定基础。方法:利用融合表达载体将海蛇神经毒素与硫氧还蛋白融合表达,使其在胞内可溶;采用亲和层析与G5... 目的:将海蛇神经毒素基因克隆到融合表达载体pET32a中,诱导表达海蛇神经毒素,鉴定融合表达蛋白的生物功能,为大量合成海蛇神经毒素奠定基础。方法:利用融合表达载体将海蛇神经毒素与硫氧还蛋白融合表达,使其在胞内可溶;采用亲和层析与G50凝胶过滤纯化融合蛋白;利用豚鼠直肠纵肌电刺激检验融合蛋白的神经信号阻断功能。结果:得到电泳纯的融合蛋白,该蛋白对豚鼠直肠纵肌电刺激有明显的阻断作用。结论:融合表达能促进海蛇神经毒素的可溶性表达,表达产物能阻断神经信号的传递。 展开更多
关键词 半环扁尾海蛇 海蛇神经毒素 融合表达 亲和层析 凝胶过滤 直肠纵肌
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