Background: Leaves of Mangifera indica L or Lannea microcarpa Engl. & K. Krause are used in traditional medicine in Burkina Faso to treat bacterial, parasitic or metabolic diseases. Objective: The aim of this stud...Background: Leaves of Mangifera indica L or Lannea microcarpa Engl. & K. Krause are used in traditional medicine in Burkina Faso to treat bacterial, parasitic or metabolic diseases. Objective: The aim of this study is to evaluate the acute general toxicity, antioxidant potential and antibacterial activitiy of leave’s aqueous extracts from Lannea microcarpa Engl. and K. Krause and Mangifera indica L. The use of these plants in traditional medicine motivated our choice to lead scientific studies. Methods: The aqueous decoction of the leaves is the form of use recommended by traditional healers. We used the same type of extracts for studies. Acute toxicity was studied in NMRI strain mice, with the dose of 2000 mg/kg body weight, for each plant species. The antioxidant activity is evaluated by the method of reduction of radical DPPH. The phytochemical compounds were detected with specific reagent: Alcaloids with Dragendorff’s reagent, Flavonoids with ammonia (NH<sub>4</sub>OH), Polyphenols and tannins are revelated by ferric chloride (FeCl<sub>3</sub>). Saponosides were revelated by their foaming power property. Bacterial inhibiting activity is tested by measuring the diameters’ inhibition of extracts on reference strains. Results: The aqueous extracts were not toxic at the maximum dose of 2000 mg/kg body weight, for each plant species. The extracts showed an antioxidant activity with an IC50, 20 μg/ml for Lannea microcarpa and 18 μg/ml for Mangifera indica. The extracts showed no bacterial activity on three strains of bacteria tested: Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. The phytochemicals we have identified are for L. microcarpa: tannins and phenolics compounds, triterpenes, saponosides. We identified in M. indica’s extracts: tannins and phenolics compounds, triterpenes, saponosides and flavonoids.展开更多
Background and Objectives: The species to the Lannea genus are trees, shrubs with compound leaves. Thus, individuals called atypical Lannea with single leaves and of socio-economic interests have been identified in th...Background and Objectives: The species to the Lannea genus are trees, shrubs with compound leaves. Thus, individuals called atypical Lannea with single leaves and of socio-economic interests have been identified in the central plateau region (Burkina Faso). This study aimed to contribute to the identification of atypical species. Material and Methods: The Polymerase Chain Reaction (PCR) method using specific primers was performed and consisted of extracting DNA from young leaves of Lannea individuals, amplifying and then sequencing portions of discriminating DNA (matK, rbcL and rps16). Results: It was shown that individuals belong to the Lannea genus, but are subdivided into three subgroups: a first subgroup containing Lannea microcarpa and two subgroups with no previously identified Lannea species. Conclusion: These atypical individuals are ecotypes or mutants of Lannea microcarpa.展开更多
Lannea kerstingii is known for its multiple therapeutic and biological activities. Despite of many traditional uses of this plant, scientific research on the content of its chemical compounds is still limited. This st...Lannea kerstingii is known for its multiple therapeutic and biological activities. Despite of many traditional uses of this plant, scientific research on the content of its chemical compounds is still limited. This study aims to isolate the chemical compounds contained in the n-butanol fraction of Lannea kerstingii leaves. The chemical investigation of the leaves of Lannea kerstingii led to isolation of three undescribed C-13 norisoprenoids, lankerstinol A-C (1-3), together with six (4-9) known flavonoid glycosides. The structures of these compounds were established by spectroscopic analyses.展开更多
Lannea velutina is a traditional herbal remedy used to treat various diseases, including hypertension (HBP). Herbal medicines are affordable, and some have minimal side effects;they are rich in bioactive components th...Lannea velutina is a traditional herbal remedy used to treat various diseases, including hypertension (HBP). Herbal medicines are affordable, and some have minimal side effects;they are rich in bioactive components that encourage prevention and treatment. This work uses appropriate experimental paradigms to investigate the phytochemical composition, antioxidant activity, and acute oral toxicity of L. velutina trunk bark extracted in water, methanol, ethyl acetate, dichloromethane, and hexane. According to a high-performance thin-layer chromatography profile, this shrub’s bark contains sterols, saponosides, flavonoids, and tannins. Compared to ethyl acetate and aqueous extracts, the methanol extract had the highest total phenolic (607.06 ± 0.6 mg GAE/g DW), flavonoid (20.97 ± 0.23 mg QE/g DW), and condensed tannins (194.50 ± 0.75 CE/g DW) content. The methanol extract displayed the highest antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capability assay (IC<sub>50</sub> = 8.59 g/mL;AAI = 4.66) compared to the other extracts. It exhibited antiradical action comparable to that of Trolox (IC<sub>50</sub> = 15.16 g/mL), ascorbic acid (IC<sub>50</sub> = 11.94 g/mL), and catechin (IC<sub>50</sub> = 11.64 g/mL). The connection between flavonoid concentration and hydrophilic antioxidant activity was robust (r = 0.997). Mice were used to evaluate the acute oral toxicity of ethyl acetate, methanol, and aqueous extracts using guidelines 425 of the Organization for Economic Cooperation and Development. All tested extracts have an estimated LD<sub>50</sub> greater than 2000 mg/kg body weight. The truncal bark of L. velutina could be an alternative source for HTA management.展开更多
Lannea microcrapa Engl. & K. Krause (Anacardiaceae) is a fruit and medicinal plant widely used in Burkina Faso. This plant is traditionally used in the treatment of hypertension. The aim of the present study was t...Lannea microcrapa Engl. & K. Krause (Anacardiaceae) is a fruit and medicinal plant widely used in Burkina Faso. This plant is traditionally used in the treatment of hypertension. The aim of the present study was to evaluate the vasorelaxant effects of the hydroethanolic extract from Lannea microcarpa trunk barks (HE_ELM) on the aorta isolated from NMRI mice. Phytochemical screening by HPTLC, assay of phenolic and flavonoid compounds, assessment of antioxidant activity (DPPH, ABTS, FRAP, and LPO), and myography of HE_ELM (1 - 2000 μg/mL) on mice thoracic aortas in the presence and absence of endothelium were carried out. Endothelium-dependent and endothelium-independant vasodilation were assessed by cumulative addition of Ach (1 nM - 10 μM) on aortic rings precontracted with the thromboxane analogue A2 agonist, 9,11-dideoxy9α,11α-methanoepoxy PGF2α (U46619). L-NAME was used to verify the involvement of NO production in the relaxation mechanism of the extract. Acute oral toxicity of HE_ELM was also evaluated. A phytochemical study revealed the presence of tannins, flavonoids, sterols and triterpenes, saponosides, and high levels of total phenolics and flavonoids. These compounds are thought to be responsible for the extract’s antioxidant and vasorelaxant properties. HE_ELM demonstrated significant antioxidant potential and induced aortic relaxation. Indeed, pharmacological parameters gave EC<sub>50</sub> values ranging from 596.45 ± 95.82 μg/mL to 749.48 ± 133.40 μg/mL and Emax values from 85.51% ± 9.59% to 96.81% ± 8.60% for the three conditions of vasodilation of the extract (p > 0.05). A complete antagonism of the contractile effect of U46619 was noted with 1 mg/mL HE_ELM. These results suggest that HE_ELM induces aortic relaxation through a concentration-dependent, endothelium-independent mechanism, possibly involving intracellular calcium mobilization of vascular cells. Acute oral toxicity tests of HE_ELM (2000 mg/kg) showed no mortality or adverse effects, suggesting the extract’s safety and potential as a therapeutic agent for hypertension. This discovery scientifically validates the use of the plant in alternative medicine to treat hypertension.展开更多
Aim: The harmful effects of pesticides have been largely documented in recent times. But effective therapeutic solutions to pesticide related male infertility are yet to be established. This study investigated the cur...Aim: The harmful effects of pesticides have been largely documented in recent times. But effective therapeutic solutions to pesticide related male infertility are yet to be established. This study investigated the curative effects of Lannea acida on imidacloprid (IMI)-induced hypofertility in male Wistar rats. Methods: Rats of 150 – 200 g were administered IMI (22.5 mg/kg) for two weeks and partitioned into control (distilled water, vitamin E, clomiphene citrate) or test (aqueous (340 mg/kg), methanol (170 mg/kg) extract) groups for eight weeks treatment. Animals were sacrificed at the end of the treatment and samples were collected for sperm, antioxidant and hormonal analysis. Fertility tests were performed from treatment day 47 for fertility indices estimation. Results were expressed as mean ± SEM and one way ANOVA was applied using STATISTICA Software. Results: Exposition to IMI resulted in a significant decrease in sperm count, motility, viability and normality, testosterone and LH, coupled to an increase in oxidative stress markers. Moreover, IMI impaired male fertility evidenced by a significant drop in fertility index and litter size. Similar to clomiphene citrate and vitamin E, plant extracts significantly improved the sperm parameters, sexual hormones and decreased the oxidative stress markers. More importantly, the fertility index and litter size were restored, especially with the aqueous extract. Conclusion: Present results indicate that L. acida possesses curative potentials against IMI-induced hypofertility through its androgenic and antioxidant properties. However, the effects the extract on spermatozoa DNA structure and the fertility of offsprings from exposed parents are yet to be studied to conclude on total recovery from IMI toxicity.展开更多
Objective To study the chemical constituents from the barks of Lannea coromande/ica. Methods The chemical constituents were isolated and purified by column chromatography on silica gel column. NMR spectra were used fo...Objective To study the chemical constituents from the barks of Lannea coromande/ica. Methods The chemical constituents were isolated and purified by column chromatography on silica gel column. NMR spectra were used for structural identification. Results Thirteen compounds were isolated and identified as quercetin （1）, （2S,3S,4R, lOLg-2-[（2＂R）-2＇-hydroxytetracosanoyl amino]-10-octadecene-l,3,4- triol （2）, aralia cerebroside （3）, 5,5＇-dibuthoxy-2,2＇-bifuran （4）, ^-sitosteryl- 3^-glucopyranoside-6＇-O-palmitate （5）, B-sitosterol palmitate （6）, myricadiol （7）, protocatechuic acid （8）, p-hydroxybenzoic acidethyl ester （9）, isovanillin （10）, trans- cinnamicacid （I 1）, palmiticacid （12）, and stearicacid （13）. Conclusion Compounds 2-13 are isolated from this plant for the first time.展开更多
In light of the use of Lannea barteri in the management of diverse illnesses and treatment of wounds in traditional medicine, the current study was conducted to assess the wound-healing efficacy of crude aqueous extra...In light of the use of Lannea barteri in the management of diverse illnesses and treatment of wounds in traditional medicine, the current study was conducted to assess the wound-healing efficacy of crude aqueous extract of the stem bark of Lannea barteri and its shea butter formulated ointment using an excision wound model in the rabbit. The herbal ointment (5%, w/w) exhibited a significant wound healing activity, showing 99.9% ± 0.3% wound contraction at the end of the experiment (24th day). There was no significant difference (p > 0.05) between the 5% (w/w) herbal ointment compared with Silver Sulphadiazine cream (positive control), which showed a 97.8% ± 1.0% rate of excision wound contraction on day 24. Compared with the negative control group (administered with only shea butter), the wound healing activity of the ointment was significant (p Lannea barteri for the treatment of wounds and demonstrates that shea butter usage as a base in formulating an herbal ointment might aid in topical application for wound repair and regeneration, as well as the potential for enhanced wound healing.展开更多
文摘Background: Leaves of Mangifera indica L or Lannea microcarpa Engl. & K. Krause are used in traditional medicine in Burkina Faso to treat bacterial, parasitic or metabolic diseases. Objective: The aim of this study is to evaluate the acute general toxicity, antioxidant potential and antibacterial activitiy of leave’s aqueous extracts from Lannea microcarpa Engl. and K. Krause and Mangifera indica L. The use of these plants in traditional medicine motivated our choice to lead scientific studies. Methods: The aqueous decoction of the leaves is the form of use recommended by traditional healers. We used the same type of extracts for studies. Acute toxicity was studied in NMRI strain mice, with the dose of 2000 mg/kg body weight, for each plant species. The antioxidant activity is evaluated by the method of reduction of radical DPPH. The phytochemical compounds were detected with specific reagent: Alcaloids with Dragendorff’s reagent, Flavonoids with ammonia (NH<sub>4</sub>OH), Polyphenols and tannins are revelated by ferric chloride (FeCl<sub>3</sub>). Saponosides were revelated by their foaming power property. Bacterial inhibiting activity is tested by measuring the diameters’ inhibition of extracts on reference strains. Results: The aqueous extracts were not toxic at the maximum dose of 2000 mg/kg body weight, for each plant species. The extracts showed an antioxidant activity with an IC50, 20 μg/ml for Lannea microcarpa and 18 μg/ml for Mangifera indica. The extracts showed no bacterial activity on three strains of bacteria tested: Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. The phytochemicals we have identified are for L. microcarpa: tannins and phenolics compounds, triterpenes, saponosides. We identified in M. indica’s extracts: tannins and phenolics compounds, triterpenes, saponosides and flavonoids.
文摘Background and Objectives: The species to the Lannea genus are trees, shrubs with compound leaves. Thus, individuals called atypical Lannea with single leaves and of socio-economic interests have been identified in the central plateau region (Burkina Faso). This study aimed to contribute to the identification of atypical species. Material and Methods: The Polymerase Chain Reaction (PCR) method using specific primers was performed and consisted of extracting DNA from young leaves of Lannea individuals, amplifying and then sequencing portions of discriminating DNA (matK, rbcL and rps16). Results: It was shown that individuals belong to the Lannea genus, but are subdivided into three subgroups: a first subgroup containing Lannea microcarpa and two subgroups with no previously identified Lannea species. Conclusion: These atypical individuals are ecotypes or mutants of Lannea microcarpa.
文摘Lannea kerstingii is known for its multiple therapeutic and biological activities. Despite of many traditional uses of this plant, scientific research on the content of its chemical compounds is still limited. This study aims to isolate the chemical compounds contained in the n-butanol fraction of Lannea kerstingii leaves. The chemical investigation of the leaves of Lannea kerstingii led to isolation of three undescribed C-13 norisoprenoids, lankerstinol A-C (1-3), together with six (4-9) known flavonoid glycosides. The structures of these compounds were established by spectroscopic analyses.
文摘Lannea velutina is a traditional herbal remedy used to treat various diseases, including hypertension (HBP). Herbal medicines are affordable, and some have minimal side effects;they are rich in bioactive components that encourage prevention and treatment. This work uses appropriate experimental paradigms to investigate the phytochemical composition, antioxidant activity, and acute oral toxicity of L. velutina trunk bark extracted in water, methanol, ethyl acetate, dichloromethane, and hexane. According to a high-performance thin-layer chromatography profile, this shrub’s bark contains sterols, saponosides, flavonoids, and tannins. Compared to ethyl acetate and aqueous extracts, the methanol extract had the highest total phenolic (607.06 ± 0.6 mg GAE/g DW), flavonoid (20.97 ± 0.23 mg QE/g DW), and condensed tannins (194.50 ± 0.75 CE/g DW) content. The methanol extract displayed the highest antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capability assay (IC<sub>50</sub> = 8.59 g/mL;AAI = 4.66) compared to the other extracts. It exhibited antiradical action comparable to that of Trolox (IC<sub>50</sub> = 15.16 g/mL), ascorbic acid (IC<sub>50</sub> = 11.94 g/mL), and catechin (IC<sub>50</sub> = 11.64 g/mL). The connection between flavonoid concentration and hydrophilic antioxidant activity was robust (r = 0.997). Mice were used to evaluate the acute oral toxicity of ethyl acetate, methanol, and aqueous extracts using guidelines 425 of the Organization for Economic Cooperation and Development. All tested extracts have an estimated LD<sub>50</sub> greater than 2000 mg/kg body weight. The truncal bark of L. velutina could be an alternative source for HTA management.
文摘Lannea microcrapa Engl. & K. Krause (Anacardiaceae) is a fruit and medicinal plant widely used in Burkina Faso. This plant is traditionally used in the treatment of hypertension. The aim of the present study was to evaluate the vasorelaxant effects of the hydroethanolic extract from Lannea microcarpa trunk barks (HE_ELM) on the aorta isolated from NMRI mice. Phytochemical screening by HPTLC, assay of phenolic and flavonoid compounds, assessment of antioxidant activity (DPPH, ABTS, FRAP, and LPO), and myography of HE_ELM (1 - 2000 μg/mL) on mice thoracic aortas in the presence and absence of endothelium were carried out. Endothelium-dependent and endothelium-independant vasodilation were assessed by cumulative addition of Ach (1 nM - 10 μM) on aortic rings precontracted with the thromboxane analogue A2 agonist, 9,11-dideoxy9α,11α-methanoepoxy PGF2α (U46619). L-NAME was used to verify the involvement of NO production in the relaxation mechanism of the extract. Acute oral toxicity of HE_ELM was also evaluated. A phytochemical study revealed the presence of tannins, flavonoids, sterols and triterpenes, saponosides, and high levels of total phenolics and flavonoids. These compounds are thought to be responsible for the extract’s antioxidant and vasorelaxant properties. HE_ELM demonstrated significant antioxidant potential and induced aortic relaxation. Indeed, pharmacological parameters gave EC<sub>50</sub> values ranging from 596.45 ± 95.82 μg/mL to 749.48 ± 133.40 μg/mL and Emax values from 85.51% ± 9.59% to 96.81% ± 8.60% for the three conditions of vasodilation of the extract (p > 0.05). A complete antagonism of the contractile effect of U46619 was noted with 1 mg/mL HE_ELM. These results suggest that HE_ELM induces aortic relaxation through a concentration-dependent, endothelium-independent mechanism, possibly involving intracellular calcium mobilization of vascular cells. Acute oral toxicity tests of HE_ELM (2000 mg/kg) showed no mortality or adverse effects, suggesting the extract’s safety and potential as a therapeutic agent for hypertension. This discovery scientifically validates the use of the plant in alternative medicine to treat hypertension.
文摘Aim: The harmful effects of pesticides have been largely documented in recent times. But effective therapeutic solutions to pesticide related male infertility are yet to be established. This study investigated the curative effects of Lannea acida on imidacloprid (IMI)-induced hypofertility in male Wistar rats. Methods: Rats of 150 – 200 g were administered IMI (22.5 mg/kg) for two weeks and partitioned into control (distilled water, vitamin E, clomiphene citrate) or test (aqueous (340 mg/kg), methanol (170 mg/kg) extract) groups for eight weeks treatment. Animals were sacrificed at the end of the treatment and samples were collected for sperm, antioxidant and hormonal analysis. Fertility tests were performed from treatment day 47 for fertility indices estimation. Results were expressed as mean ± SEM and one way ANOVA was applied using STATISTICA Software. Results: Exposition to IMI resulted in a significant decrease in sperm count, motility, viability and normality, testosterone and LH, coupled to an increase in oxidative stress markers. Moreover, IMI impaired male fertility evidenced by a significant drop in fertility index and litter size. Similar to clomiphene citrate and vitamin E, plant extracts significantly improved the sperm parameters, sexual hormones and decreased the oxidative stress markers. More importantly, the fertility index and litter size were restored, especially with the aqueous extract. Conclusion: Present results indicate that L. acida possesses curative potentials against IMI-induced hypofertility through its androgenic and antioxidant properties. However, the effects the extract on spermatozoa DNA structure and the fertility of offsprings from exposed parents are yet to be studied to conclude on total recovery from IMI toxicity.
基金National Natural Science Foundation(21162009)Special Major Science and Technology R&D in Hainan(ZDZX20100007)
文摘Objective To study the chemical constituents from the barks of Lannea coromande/ica. Methods The chemical constituents were isolated and purified by column chromatography on silica gel column. NMR spectra were used for structural identification. Results Thirteen compounds were isolated and identified as quercetin （1）, （2S,3S,4R, lOLg-2-[（2＂R）-2＇-hydroxytetracosanoyl amino]-10-octadecene-l,3,4- triol （2）, aralia cerebroside （3）, 5,5＇-dibuthoxy-2,2＇-bifuran （4）, ^-sitosteryl- 3^-glucopyranoside-6＇-O-palmitate （5）, B-sitosterol palmitate （6）, myricadiol （7）, protocatechuic acid （8）, p-hydroxybenzoic acidethyl ester （9）, isovanillin （10）, trans- cinnamicacid （I 1）, palmiticacid （12）, and stearicacid （13）. Conclusion Compounds 2-13 are isolated from this plant for the first time.
文摘In light of the use of Lannea barteri in the management of diverse illnesses and treatment of wounds in traditional medicine, the current study was conducted to assess the wound-healing efficacy of crude aqueous extract of the stem bark of Lannea barteri and its shea butter formulated ointment using an excision wound model in the rabbit. The herbal ointment (5%, w/w) exhibited a significant wound healing activity, showing 99.9% ± 0.3% wound contraction at the end of the experiment (24th day). There was no significant difference (p > 0.05) between the 5% (w/w) herbal ointment compared with Silver Sulphadiazine cream (positive control), which showed a 97.8% ± 1.0% rate of excision wound contraction on day 24. Compared with the negative control group (administered with only shea butter), the wound healing activity of the ointment was significant (p Lannea barteri for the treatment of wounds and demonstrates that shea butter usage as a base in formulating an herbal ointment might aid in topical application for wound repair and regeneration, as well as the potential for enhanced wound healing.
