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DNA与蛋白质结合的荧光测定 被引量:2
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作者 王志珍 《生物化学杂志》 CSCD 1994年第4期427-432,共6页
构建了插入λ阻抑蛋白(Rep)的操纵基因(OR)和BglⅡ识别位点的PBR322重组质粒。阻抑蛋白与该质粒的相互作用可用BglⅡ对它的水解作用引起的EB荧光变化来研究。在E.coli中表达的Rep表现了与该重组质粒结... 构建了插入λ阻抑蛋白(Rep)的操纵基因(OR)和BglⅡ识别位点的PBR322重组质粒。阻抑蛋白与该质粒的相互作用可用BglⅡ对它的水解作用引起的EB荧光变化来研究。在E.coli中表达的Rep表现了与该重组质粒结合的活力。核苷酸序列具精确二重对称性的OR(ORcons)对Rep的亲和力比天然的OR1小。 展开更多
关键词 DNA 蛋白 荧光 测定
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A Partial Evaluator for A Parallel Lambda Language
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作者 旷海蓉 孙永强 林凯 《Journal of Computer Science & Technology》 SCIE EI CSCD 1997年第5期441-457,共17页
This paper describes theoretical and practical aspects of a partial evaluator that treats a parallel lambda language. The parallel language presented is a combination of lambda calculus and message passing communicati... This paper describes theoretical and practical aspects of a partial evaluator that treats a parallel lambda language. The parallel language presented is a combination of lambda calculus and message passing communication mechanism. This parallel language can be used to write a programming language's denotational semantics which extracts the parallelism in the program. From this denotational definition of the programming language, the partial evaluator can generate parallel compiler of the language by self application.The key technique of partial evaluation is binding time analysis that determines in advance which parts of the source program can be evaluated during partial evaluation, and which parts cannot. A binding time analysis is described based upon type inference. A new type chcode is introduced into the type system, which denotes the type of those expressions containing residual channel operations. A well-formedness criterion is given which ensures that partial evaluation not only doesn't commit type errors but also doesn't change the sequence of channel operations. Before binding time analysis, channel analysis is used to analyze the communication relationship between send and receive processes. 展开更多
关键词 Partial evaluation binding time analysis parallel lambda language
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