BACKGROUND Although an association between gut microbiota and cholestatic liver disease(CLD)has been reported,the precise functional roles of these microbes in CLD pathogenesis remain largely unknown.AIM To explore th...BACKGROUND Although an association between gut microbiota and cholestatic liver disease(CLD)has been reported,the precise functional roles of these microbes in CLD pathogenesis remain largely unknown.AIM To explore the function of gut microbes in CLD pathogenesis and the effects of gut microbiota on intestinal barrier and bile acid(BA)metabolism in CLD.METHODS Male C57BL/6J mice were fed a 0.05%3,5-diethoxycarbonyl-1,4-dihydrocollidine diet for 2 weeks to induce CLD.The sterile liver tissues of mice were then meticulously harvested,and bacteria in homogenates were identified through culture methods.Furthermore,16S ribosomal DNA sequencing was employed to analyze sterile liver samples collected from eight patients with primary biliary cholangitis(PBC)and three control individuals with hepatic cysts.The functional roles of the identified bacteria in CLD pathogenesis were assessed through microbiota transfer experiments,involving the evaluation of changes in intestinal permeability and BA dynamics.RESULTS Ligilactobacillus murinus(L.murinus)and Lactococcus garvieae(L.garvieae)were isolated from the bacterial culture of livers from CLD mice.L.murinus was prevalently detected in PBC patients and controls,whereas L.garvieae was detected only in patients with PBC but not in controls.Mice inoculated with L.garvieae exhibited increased susceptibility to experimental CLD,with both in vitro and in vivo indicating that L.garvieae disrupted the intestinal barrier function by down-regulating the expression of occludin and zonula occludens-1.Moreover,L.garvieae administration significantly upregulated the expression of the apical sodium-dependent BA transporter in the terminal ileum and increased serum BA levels.CONCLUSION L.garvieae contributes to excessive BA-induced hepatobiliary injury and liver fibrosis by increasing intestinal permeability and enhancing BA reabsorption.展开更多
Lactococcus garvieae is a known fish pathogen associated with numerous aquacultural outbreaks.In humans,L.garvieae primarily causes infective endocarditis,but infections involving other organs have also been reported....Lactococcus garvieae is a known fish pathogen associated with numerous aquacultural outbreaks.In humans,L.garvieae primarily causes infective endocarditis,but infections involving other organs have also been reported.We report the first case of ruptured infectious intracranial aneurysm associated with L.garvieae bacteraemia without concomitant infective endocarditis.The diagnosis of a left distal posterior cerebral artery mycotic aneurysm was based on a computed tomography angiogram,catheter angiogram and histopathological examination of the resected aneurysm.Here,we review the literature on human L.garvieae infections and describe the clinical characteristics,risk factors,management and outcomes of the cases identified to date.展开更多
Probiotics are live microbial food supplements that have been shown to have beneficial effects on animal health.Endogenous probiotic bacteria have long been used for their proposed health promoting properties and have...Probiotics are live microbial food supplements that have been shown to have beneficial effects on animal health.Endogenous probiotic bacteria have long been used for their proposed health promoting properties and have become a hot research topic in growth improvement in aquaculture.The endogenous probiotic bacteria from intestines of Macrobrachium rosenbergii(giant river prawn)was explored for their probiotic potential,from which 367 bacterial strains were isolated from the intestine of M.rosenbergii.After 16 S rDNA sequence analysis,234 isolates were identified as Lactococcus garvieae,which accounted for 63.76%of the total number of culturable intestinal bacteria,suggesting that this bacterium was the main component of the microbiota.Furthermore,to reveal the probiotic properties of L.garvieae,this isolated bacterial strain was characterized morphologically,physiologically,and biochemically.Its enzyme production capacity,bacteriostatic activity,and resistance to acid,high temperature,and pH,were assessed.In vitro experiments showed that the L.garvieae(No.C 6 a 2)had a fast growth rate and entered the logarithmic phase rapidly.Besides,it had characteristics of acid-production and resistance,enzyme-producing capacity,and strong antibacterial activity against pathogenic Staphylococc us aureus,Aeromonas hydrophila,and Aeromonas veronii.However,it lacked the ability to tolerate high temperature.Our results provide novel data to deepen our understanding of the intestinal bacteria structure of M.rosenbergii and valuable information for probiotic screening and the application for M.rosenbergii.展开更多
基金Supported by Tianjin Health Research Project,No.TJWJ2024QN005Beijing iGandan Public Welfare Foundation Artificial Liver Special Fund,No.iGandanF-1082024-RGG122.
文摘BACKGROUND Although an association between gut microbiota and cholestatic liver disease(CLD)has been reported,the precise functional roles of these microbes in CLD pathogenesis remain largely unknown.AIM To explore the function of gut microbes in CLD pathogenesis and the effects of gut microbiota on intestinal barrier and bile acid(BA)metabolism in CLD.METHODS Male C57BL/6J mice were fed a 0.05%3,5-diethoxycarbonyl-1,4-dihydrocollidine diet for 2 weeks to induce CLD.The sterile liver tissues of mice were then meticulously harvested,and bacteria in homogenates were identified through culture methods.Furthermore,16S ribosomal DNA sequencing was employed to analyze sterile liver samples collected from eight patients with primary biliary cholangitis(PBC)and three control individuals with hepatic cysts.The functional roles of the identified bacteria in CLD pathogenesis were assessed through microbiota transfer experiments,involving the evaluation of changes in intestinal permeability and BA dynamics.RESULTS Ligilactobacillus murinus(L.murinus)and Lactococcus garvieae(L.garvieae)were isolated from the bacterial culture of livers from CLD mice.L.murinus was prevalently detected in PBC patients and controls,whereas L.garvieae was detected only in patients with PBC but not in controls.Mice inoculated with L.garvieae exhibited increased susceptibility to experimental CLD,with both in vitro and in vivo indicating that L.garvieae disrupted the intestinal barrier function by down-regulating the expression of occludin and zonula occludens-1.Moreover,L.garvieae administration significantly upregulated the expression of the apical sodium-dependent BA transporter in the terminal ileum and increased serum BA levels.CONCLUSION L.garvieae contributes to excessive BA-induced hepatobiliary injury and liver fibrosis by increasing intestinal permeability and enhancing BA reabsorption.
文摘Lactococcus garvieae is a known fish pathogen associated with numerous aquacultural outbreaks.In humans,L.garvieae primarily causes infective endocarditis,but infections involving other organs have also been reported.We report the first case of ruptured infectious intracranial aneurysm associated with L.garvieae bacteraemia without concomitant infective endocarditis.The diagnosis of a left distal posterior cerebral artery mycotic aneurysm was based on a computed tomography angiogram,catheter angiogram and histopathological examination of the resected aneurysm.Here,we review the literature on human L.garvieae infections and describe the clinical characteristics,risk factors,management and outcomes of the cases identified to date.
基金Supported by the National Natural Science Foundation of China(No.32273121)the Natural Science Foundation of Zhejiang Province(No.LGN22C190019)+1 种基金the Huzhou Natural Science Foundation(No.2021YZ08)the earmarked fund for the China Agriculture Research System of MOF and MARA(No.CARS-48)。
文摘Probiotics are live microbial food supplements that have been shown to have beneficial effects on animal health.Endogenous probiotic bacteria have long been used for their proposed health promoting properties and have become a hot research topic in growth improvement in aquaculture.The endogenous probiotic bacteria from intestines of Macrobrachium rosenbergii(giant river prawn)was explored for their probiotic potential,from which 367 bacterial strains were isolated from the intestine of M.rosenbergii.After 16 S rDNA sequence analysis,234 isolates were identified as Lactococcus garvieae,which accounted for 63.76%of the total number of culturable intestinal bacteria,suggesting that this bacterium was the main component of the microbiota.Furthermore,to reveal the probiotic properties of L.garvieae,this isolated bacterial strain was characterized morphologically,physiologically,and biochemically.Its enzyme production capacity,bacteriostatic activity,and resistance to acid,high temperature,and pH,were assessed.In vitro experiments showed that the L.garvieae(No.C 6 a 2)had a fast growth rate and entered the logarithmic phase rapidly.Besides,it had characteristics of acid-production and resistance,enzyme-producing capacity,and strong antibacterial activity against pathogenic Staphylococc us aureus,Aeromonas hydrophila,and Aeromonas veronii.However,it lacked the ability to tolerate high temperature.Our results provide novel data to deepen our understanding of the intestinal bacteria structure of M.rosenbergii and valuable information for probiotic screening and the application for M.rosenbergii.
