The hydrogen-producing consortium conveying the lactate-fermentation pathway was enriched and used as a coinoculum with the non-enriched hydrogen-producing consortium for biohydrogen production in the presence of lact...The hydrogen-producing consortium conveying the lactate-fermentation pathway was enriched and used as a coinoculum with the non-enriched hydrogen-producing consortium for biohydrogen production in the presence of lactic acid bacteria(LAB).The co-inoculum treatment achieved superior hydrogen production performance compared to that of the non-enriched consortium treatment.The effects of enriched consortium concentration,initial pH,and glucose concentration were evaluated,and hydrogen production potential(HP)of 1,605±161 mL-H_(2)/L and a maximum hydrogen production rate(HPR)of 87.17±15.85 mL-H_(2)/L.h were achieved under optimal conditions.Biohydrogen production from food waste using the co-inoculum was 1,137 mL-H_(2)/L from non-autoclaved food waste,corresponding to 56.85 mL-H_(2)/g-VS_(added).Metabolite product and microbial community analyses during food waste fermentation indicated positive cross-feeding activity of hydrogen producers,LAB,and acetogenic bacteria.This study provides valuable information on the use of an efficient,enriched hydrogen-producing consortium to improve biohydrogen production from LAB-contaminated feedstock.展开更多
基金funded by the Fundamental Fund of Khon Kaen University from the National Science,Research and Innovation Fund(NSRF)the Research Group for Development of Microbial Hydrogen Production Process from Biomass,Khon Kaen University,Thailand.
文摘The hydrogen-producing consortium conveying the lactate-fermentation pathway was enriched and used as a coinoculum with the non-enriched hydrogen-producing consortium for biohydrogen production in the presence of lactic acid bacteria(LAB).The co-inoculum treatment achieved superior hydrogen production performance compared to that of the non-enriched consortium treatment.The effects of enriched consortium concentration,initial pH,and glucose concentration were evaluated,and hydrogen production potential(HP)of 1,605±161 mL-H_(2)/L and a maximum hydrogen production rate(HPR)of 87.17±15.85 mL-H_(2)/L.h were achieved under optimal conditions.Biohydrogen production from food waste using the co-inoculum was 1,137 mL-H_(2)/L from non-autoclaved food waste,corresponding to 56.85 mL-H_(2)/g-VS_(added).Metabolite product and microbial community analyses during food waste fermentation indicated positive cross-feeding activity of hydrogen producers,LAB,and acetogenic bacteria.This study provides valuable information on the use of an efficient,enriched hydrogen-producing consortium to improve biohydrogen production from LAB-contaminated feedstock.
