A recent study by Li et al.[1]was published in Nature and proposed the crucial roles of lactylation in regulating innate immunity.Alanyl-tRNA synthetase 1(AARS1)and AARS2,the intracellular L-lactate sensors,were demon...A recent study by Li et al.[1]was published in Nature and proposed the crucial roles of lactylation in regulating innate immunity.Alanyl-tRNA synthetase 1(AARS1)and AARS2,the intracellular L-lactate sensors,were demonstrated to directly conjugate L-lactate to a lysine residue of substrate proteins.AARS2interacts with cGAS and mediates its lactylation,dampening cGAS-mediated innate antiviral response.展开更多
Lactate,as a metabolite,plays a significant role in a number of fields,including medical diagnostics,exercise physiology and food science.Traditional methods for lactate measurement often involve expensive and cumbers...Lactate,as a metabolite,plays a significant role in a number of fields,including medical diagnostics,exercise physiology and food science.Traditional methods for lactate measurement often involve expensive and cumbersome instrumentation.This study developed a portable and low-cost lactate measurement system,including independently detectable hardware circuits and user-friendly embedded software,computer,and smartphone applications.The experiment verified that the relative error of the detection current in the device circuit was less than 1%.The electrochemical performance was measured by comparing the[Fe(CN)_(6)]^(3−)/[Fe(CN)_(6)]^(4−)solution with the desktop electrochemical workstation CHI660E,and a nearly consistent chronoamperometry(CA)curve was obtained.Two modified lactate sensors were used for CA testing of lactate.Within the concentration range of 0.1 mmol·L^(−1)to 20 mmol·L^(−1),there was a good linear relationship between lactate concentration and steady-state current,with a correlation coefficient(R2)greater than 0.99 and good repeatability,demonstrating the reliability of the developed device.The lactate measurement system developed in this study not only provides excellent detection performance and reliability,but also achieves portability and low cost,providing a new solution for lactate measurement.展开更多
基金supported by the National Natural Science Foundation of China(32270975,32070907,U24A20371,and T2321005)the National Key R&D Program of China(2023YFA1800200 and 2022YFA1105200)+3 种基金the National Science Fund for Distinguished Young Scholars(32125016)Suzhou Medical College basic frontier innovation cross project(YXY2303027)Suzhou Innovation and Entrepreneurship Leading Talent Program(ZXL2022505)the Priority Acadamic Program Development of Jiangsu Higher Education Institutions(PAPD)。
文摘A recent study by Li et al.[1]was published in Nature and proposed the crucial roles of lactylation in regulating innate immunity.Alanyl-tRNA synthetase 1(AARS1)and AARS2,the intracellular L-lactate sensors,were demonstrated to directly conjugate L-lactate to a lysine residue of substrate proteins.AARS2interacts with cGAS and mediates its lactylation,dampening cGAS-mediated innate antiviral response.
基金supported by National Natural Science Foundation of China(No.62006092)Natural Science Research Project of Anhui Educational Committee(No.2023AH030081)+1 种基金2023 New Era Education Provincial Quality Engineering Project(Graduate Education)(No.2023cxcysj103)2024 New Era Education Provincial Quality Engineering Project(Graduate Education)。
文摘Lactate,as a metabolite,plays a significant role in a number of fields,including medical diagnostics,exercise physiology and food science.Traditional methods for lactate measurement often involve expensive and cumbersome instrumentation.This study developed a portable and low-cost lactate measurement system,including independently detectable hardware circuits and user-friendly embedded software,computer,and smartphone applications.The experiment verified that the relative error of the detection current in the device circuit was less than 1%.The electrochemical performance was measured by comparing the[Fe(CN)_(6)]^(3−)/[Fe(CN)_(6)]^(4−)solution with the desktop electrochemical workstation CHI660E,and a nearly consistent chronoamperometry(CA)curve was obtained.Two modified lactate sensors were used for CA testing of lactate.Within the concentration range of 0.1 mmol·L^(−1)to 20 mmol·L^(−1),there was a good linear relationship between lactate concentration and steady-state current,with a correlation coefficient(R2)greater than 0.99 and good repeatability,demonstrating the reliability of the developed device.The lactate measurement system developed in this study not only provides excellent detection performance and reliability,but also achieves portability and low cost,providing a new solution for lactate measurement.
