长链酰基辅酶A合成酶(long-chain acyl-coenzyme A synthetase,LACS)在脂肪酸代谢、角质层角质和蜡的合成中发挥着重要作用。为研究小麦中LACS基因的特征,以中国春为材料,进行全基因组鉴定和分析。结果共鉴定出148个小麦LACS基因(TaLAC...长链酰基辅酶A合成酶(long-chain acyl-coenzyme A synthetase,LACS)在脂肪酸代谢、角质层角质和蜡的合成中发挥着重要作用。为研究小麦中LACS基因的特征,以中国春为材料,进行全基因组鉴定和分析。结果共鉴定出148个小麦LACS基因(TaLACS),不均匀分布于21条染色体上,且有成簇分布的现象,编码蛋白大部分为中性、亲水的水溶性蛋白,性质稳定。系统进化分析结果显示,148个TaLACS基因聚为6组,有55对旁系同源基因。表达谱分析表明,TaLACS基因在不同组织和逆境胁迫下存在明显的差异表达,说明TaLACS基因具有组织特异性,在抗低温、干旱和赤霉病过程中TaLACS基因发挥重要作用。该家族中含有响应低温、干旱和脱落酸元件的基因分别占34.9%、46.0%和85.8%,揭示TaLACS基因参与生物胁迫和非生物胁迫过程。展开更多
Long-chain acyl-Coenzyme A （CoA） synthetases （LACSs） catalyze the formation of long-chain acyl-CoA, and play important roles in fatty acid metabolism including phospholipids, triacylglycerol （TAG） biosynthesis a...Long-chain acyl-Coenzyme A （CoA） synthetases （LACSs） catalyze the formation of long-chain acyl-CoA, and play important roles in fatty acid metabolism including phospholipids, triacylglycerol （TAG） biosynthesis and fatty acid 13-oxidation. Here, we report the characterization of a LACS gene from Brassica napus. It is highly homologous to Arabidopsis LACS4 and thus designated as BnLACS4. The cloned gene BnLACS4 could complement a LACS-deficient yeast strain YB525. It is mainly expressed in flowers and developing seeds where lipid biosynthesis is at high rate in Brassiea napus. When transiently expressed in tobacco leaves, BnLACS4 is localized in endoplasmic reticulum （ER）, the common site for eukaryotic pathway of lipid biosynthesis. Expression of BnLACS4 in the yeast strain pep4 increased its lipid content. Taken together, our results suggest that BnLACS4 may be involved in lipid biosynthesis in B. napus.展开更多
Long-chain acyl-CoA synthetase(LACS),belonging to the AMP-bound superfamily,catalyzes the activation of long-chain fatty acids to their coenzyme A esters.Candidate Gene Association analysis showed that LACS8 was not o...Long-chain acyl-CoA synthetase(LACS),belonging to the AMP-bound superfamily,catalyzes the activation of long-chain fatty acids to their coenzyme A esters.Candidate Gene Association analysis showed that LACS8 was not only related to the content of fatty acid,but also,surprisingly,to the content of glucosinolates.Here,we cloned BnaLACS8A03 from rapeseed,and demonstrated its association with the glucosinolates content.We found that BnaLACS8A03 was widely expressed in all tissues and organs,and significantly highly expressed in leaf and flower,which are the sites of glucosinolate synthesis.Bioinformatics predicted that it was located in peroxisome,where theβ-oxidation of fatty acids mainly occurred,indicating that BnaLACS8A03 was involved in the metabolism of fatty acids.Phenotypic studies of RNA interference(RNAi)lines revealed that down-regulation of BnaLACS8A03 in rapeseed significantly reduced the glucosinolates content.In addition,the expression of glucosinolates biosynthesis-related genes and transport-related genes is altered in BnaLACS8A03-RNAi plants,suggesting that a possible crosstalk regulation mechanism exists in the regulation network of glucosinolate mechanism.Our results demonstrate that glucosinolates content can be manipulated by altering the expression of long chain acyl-Coenzyme A synthase gene BnaLACS8A03,which provide an available genetic resource for low glucosinolate breeding practice in rapeseed.展开更多
文摘长链酰基辅酶A合成酶(long-chain acyl-coenzyme A synthetase,LACS)在脂肪酸代谢、角质层角质和蜡的合成中发挥着重要作用。为研究小麦中LACS基因的特征,以中国春为材料,进行全基因组鉴定和分析。结果共鉴定出148个小麦LACS基因(TaLACS),不均匀分布于21条染色体上,且有成簇分布的现象,编码蛋白大部分为中性、亲水的水溶性蛋白,性质稳定。系统进化分析结果显示,148个TaLACS基因聚为6组,有55对旁系同源基因。表达谱分析表明,TaLACS基因在不同组织和逆境胁迫下存在明显的差异表达,说明TaLACS基因具有组织特异性,在抗低温、干旱和赤霉病过程中TaLACS基因发挥重要作用。该家族中含有响应低温、干旱和脱落酸元件的基因分别占34.9%、46.0%和85.8%,揭示TaLACS基因参与生物胁迫和非生物胁迫过程。
基金supported by the Natural Science Foundation of China(31271760)the Opening Funds of the Key Laboratory of Biology and Genetic Improvement of Oilcrops,Ministry of Agriculture,China
文摘Long-chain acyl-Coenzyme A （CoA） synthetases （LACSs） catalyze the formation of long-chain acyl-CoA, and play important roles in fatty acid metabolism including phospholipids, triacylglycerol （TAG） biosynthesis and fatty acid 13-oxidation. Here, we report the characterization of a LACS gene from Brassica napus. It is highly homologous to Arabidopsis LACS4 and thus designated as BnLACS4. The cloned gene BnLACS4 could complement a LACS-deficient yeast strain YB525. It is mainly expressed in flowers and developing seeds where lipid biosynthesis is at high rate in Brassiea napus. When transiently expressed in tobacco leaves, BnLACS4 is localized in endoplasmic reticulum （ER）, the common site for eukaryotic pathway of lipid biosynthesis. Expression of BnLACS4 in the yeast strain pep4 increased its lipid content. Taken together, our results suggest that BnLACS4 may be involved in lipid biosynthesis in B. napus.
基金supported by the National Key R&D Program of China(2016YFD0101900 and 2016YFD0100305)the National Natural Science Foundation of China(Grant No.32001582)the General Project of Natural Science Research in Colleges and Universities of Jiangsu Province(Grant No.20KJB210002)。
文摘Long-chain acyl-CoA synthetase(LACS),belonging to the AMP-bound superfamily,catalyzes the activation of long-chain fatty acids to their coenzyme A esters.Candidate Gene Association analysis showed that LACS8 was not only related to the content of fatty acid,but also,surprisingly,to the content of glucosinolates.Here,we cloned BnaLACS8A03 from rapeseed,and demonstrated its association with the glucosinolates content.We found that BnaLACS8A03 was widely expressed in all tissues and organs,and significantly highly expressed in leaf and flower,which are the sites of glucosinolate synthesis.Bioinformatics predicted that it was located in peroxisome,where theβ-oxidation of fatty acids mainly occurred,indicating that BnaLACS8A03 was involved in the metabolism of fatty acids.Phenotypic studies of RNA interference(RNAi)lines revealed that down-regulation of BnaLACS8A03 in rapeseed significantly reduced the glucosinolates content.In addition,the expression of glucosinolates biosynthesis-related genes and transport-related genes is altered in BnaLACS8A03-RNAi plants,suggesting that a possible crosstalk regulation mechanism exists in the regulation network of glucosinolate mechanism.Our results demonstrate that glucosinolates content can be manipulated by altering the expression of long chain acyl-Coenzyme A synthase gene BnaLACS8A03,which provide an available genetic resource for low glucosinolate breeding practice in rapeseed.
文摘为探讨缢蛏(Sinonovacula constricta)耐受高硫化物环境的分子机理,利用RNA-seq技术开展395μmol/L硫化物胁迫0 h、6 h和96 h缢蛏鳃转录组动态变化的研究,构建9个转录组文库,获得27.09 Gb有效数据,组装得到65747条Unigenes,平均长度为971.46 bp,N50达到1434 bp,注释得到22921条Unigenes。按照FPKM值获得基因表达丰度后,采用DEGseq软件进行差异表达分析,分别在6 h vs.0 h、96 h vs.0 h和96 h vs.6 h组中获得61138、60912和62824条DEGs。按照DEGs筛选阈值,分别在6 h vs.0 h、96 h vs.0 h和96 h vs.6 h组中获得17、10和12个高表达DEGs。KEGG分析显示差异基因(DEGs)显著富集到硫代谢通路,发现高表达的硫醌氧化还原酶基因(SQR),推测线粒体硫化物氧化是其解毒的关键。GO、KEGG分析发现DEGs分别富集到呼吸电子传递链(ETC)及氧化磷酸化通路,细胞色素b的表达显著下调,可能引发线粒体氧化呼吸链受阻,从而产生过量ROS,以激活机体的应激反应。DEGs还富集到转移含磷基团的转移酶活性、核小体组装等GO类别,推测ROS造成DNA氧化损伤后,机体通过DNA复制、组装新染色体以维持遗传物质稳定。采用qRT-PCR进行差异基因组蛋白(H2B)、漆酶(LAC)的响应研究,结果呈时间依赖性的表达模式,推测H2B通过修复受损DNA并增强免疫能力、LAC通过清除ROS以提高机体硫化物抗性。研究结果系统解析了缢蛏耐硫的分子调控机理,为缢蛏耐硫新品种的遗传育种研究奠定了理论基础。
