A simple in vitro system was employed for ectomycorrhizal synthesis of Abies firma Sieb.et Zucc.with Laccaria bicolor(Maire)Orton.The aim of this present study was to study whether the symbiosis of A.firma broad occur...A simple in vitro system was employed for ectomycorrhizal synthesis of Abies firma Sieb.et Zucc.with Laccaria bicolor(Maire)Orton.The aim of this present study was to study whether the symbiosis of A.firma broad occurred with L.bicolor and whether the symbiosis of A.firma only occurred at the whole plant level.The results of the study show that the typical ectomycorrhizal structures,i.e.thick mantle and intracortical Hartig net,were observed in the lateral roots of A.firma after incubation of 10 weeks.In case of callus,three weeks following incubation,fungal hyphae were visible within the intercellular spaces of the callus cells and Hartig net-like structures were observed in transverse section.This was the first report of aseptic ectomycorrhization of A.firma seedlings,and ectomycorrhizal colonization on A.firma callus by L.bicolor.These results suggested that the callus system might be a useful tissue system for mycorrhiza synthesis in the present study.This model system may facilitate detailed studies on ectomycorrhizal development of Abies species.展开更多
Pinus massoniana was grown in a nurse bed consisting of a strong acidic yellow soil and inoculated with 3 strains of Laccaria bicolor, namely Lb S238A, Lb S238N and Lb 270, respectively. The growth of seedlings, parti...Pinus massoniana was grown in a nurse bed consisting of a strong acidic yellow soil and inoculated with 3 strains of Laccaria bicolor, namely Lb S238A, Lb S238N and Lb 270, respectively. The growth of seedlings, particularly stems and leaves, was promoted significantly by the ectomycorrhizal(ECM) fungi in the sequence: Lb270 > Lb S238N > LbS238A. In contrast, the inhibition of aluminum translocation was observed from roots to upper parts compared to the absorption and utilization of N, P, Ca and Mg that were encouraged by ECM. Seedlings infested with Lb S238A absorbed least amount of nutrients and aluminum, while the largest amount of P, K and Mg was taken in by those with Lb 270 and the largest amount of N and Ca by those with Lb S238N。It is necessary to point out that Ca absorption and accumulation in the upper parts of pine seedling were greatly promoted by ECM inoculation. The latter in those of mycorrhizal seedlings was increased by 473%～577% over than nonmycorrhizal(NM) ones, which could benefit to the aluminum resistance of the tree seedlings. The inoculation of Laccaria bicolor could, therefore, be considered as an important and effective measurement to promote growth and nutrition and to alleviate aluminum toxicity to Pinus massoniana in south China.展开更多
基金supported by a grant from the Bio-oriented Technology Research Advancement Institution(BRAIN)in Japan.We want to thank Dr.W.M.GILL(Tasmanian Institute of Agricultural Research,Australia)for his useful discussion.The first author was also supported by a grant from Northeast Forestry University(010-602024)when this manuscript was prepared.
文摘A simple in vitro system was employed for ectomycorrhizal synthesis of Abies firma Sieb.et Zucc.with Laccaria bicolor(Maire)Orton.The aim of this present study was to study whether the symbiosis of A.firma broad occurred with L.bicolor and whether the symbiosis of A.firma only occurred at the whole plant level.The results of the study show that the typical ectomycorrhizal structures,i.e.thick mantle and intracortical Hartig net,were observed in the lateral roots of A.firma after incubation of 10 weeks.In case of callus,three weeks following incubation,fungal hyphae were visible within the intercellular spaces of the callus cells and Hartig net-like structures were observed in transverse section.This was the first report of aseptic ectomycorrhization of A.firma seedlings,and ectomycorrhizal colonization on A.firma callus by L.bicolor.These results suggested that the callus system might be a useful tissue system for mycorrhiza synthesis in the present study.This model system may facilitate detailed studies on ectomycorrhizal development of Abies species.
文摘Pinus massoniana was grown in a nurse bed consisting of a strong acidic yellow soil and inoculated with 3 strains of Laccaria bicolor, namely Lb S238A, Lb S238N and Lb 270, respectively. The growth of seedlings, particularly stems and leaves, was promoted significantly by the ectomycorrhizal(ECM) fungi in the sequence: Lb270 > Lb S238N > LbS238A. In contrast, the inhibition of aluminum translocation was observed from roots to upper parts compared to the absorption and utilization of N, P, Ca and Mg that were encouraged by ECM. Seedlings infested with Lb S238A absorbed least amount of nutrients and aluminum, while the largest amount of P, K and Mg was taken in by those with Lb 270 and the largest amount of N and Ca by those with Lb S238N。It is necessary to point out that Ca absorption and accumulation in the upper parts of pine seedling were greatly promoted by ECM inoculation. The latter in those of mycorrhizal seedlings was increased by 473%～577% over than nonmycorrhizal(NM) ones, which could benefit to the aluminum resistance of the tree seedlings. The inoculation of Laccaria bicolor could, therefore, be considered as an important and effective measurement to promote growth and nutrition and to alleviate aluminum toxicity to Pinus massoniana in south China.
