In response to the current situation of underutilization of resources for cowhide collagen,we screened a potential probiotic B.velezensis LZ676 from fermented cured pig’s trotters by using biological characteristics ...In response to the current situation of underutilization of resources for cowhide collagen,we screened a potential probiotic B.velezensis LZ676 from fermented cured pig’s trotters by using biological characteristics and whole genomics.Furthermore,the differences of functional active substances in the cowhide fermentation degradation liquid(CFDL)by this strain were investigated by means of biological activity index and untargeted metabo-nomics.The results showed that more than 68%of the peptides in CFDL were less than 3000 Da,and CFDL had remarkable in vitro antioxidant and antibacterial abilities.The untargeted metabolomics showed that the abundance of antioxidant,anti-inflammatory and anticancer related metabolites in CFDL increased,and the enrichment analysis of KEGG pathway revealed that linoleic acid metabolism was the main way to produce differential metabolites.This study provides a theoretical basis and promising strategy for the high-value pro-duction of processing by-products of livestock skin fermented by probiotic Bacillus.展开更多
基金supported by the Zhongyuan Sci-Tech Innovation Leading Talents Support Program(254000510015)General Program of National Natural Science Foundation of China(32472417)the Major science and technology projects in Henan province(231100110400).
文摘In response to the current situation of underutilization of resources for cowhide collagen,we screened a potential probiotic B.velezensis LZ676 from fermented cured pig’s trotters by using biological characteristics and whole genomics.Furthermore,the differences of functional active substances in the cowhide fermentation degradation liquid(CFDL)by this strain were investigated by means of biological activity index and untargeted metabo-nomics.The results showed that more than 68%of the peptides in CFDL were less than 3000 Da,and CFDL had remarkable in vitro antioxidant and antibacterial abilities.The untargeted metabolomics showed that the abundance of antioxidant,anti-inflammatory and anticancer related metabolites in CFDL increased,and the enrichment analysis of KEGG pathway revealed that linoleic acid metabolism was the main way to produce differential metabolites.This study provides a theoretical basis and promising strategy for the high-value pro-duction of processing by-products of livestock skin fermented by probiotic Bacillus.
