Food-derived antioxidant peptides have been shown to have beneficial effects in scavenging excess free radicals.In this study,a novel multifunctional LPxTG-motif protein LPxT-GYLEQ was synthesized,and its molecular me...Food-derived antioxidant peptides have been shown to have beneficial effects in scavenging excess free radicals.In this study,a novel multifunctional LPxTG-motif protein LPxT-GYLEQ was synthesized,and its molecular mechanism of alleviating cognitive impairment in a D-galactose(D-gal)-induced aging mice model was also investigated.The results confirmed the antioxidant effects of the LPxT-GYLEQ protein,which could scavenge excessive reactive oxygen species(ROS)in aging mice by regulating the c-Jun N-terminal kinase(JNK)/Nuclear factor erythroid 2-related factor 2(Nrf2)/p38/Nuclear factor-k-gene binding(NF-κB)signal pathway,reduced the accumulation ofβ-amyloid protein(Aβ),restored the cognitive ability of mice,improved learning and memory behavior,effectively reduced the expression of inflammatory-related factors,including tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6),and increased the expression of anti-inflammatory factor interleukin-10(IL-10).These may be related to the fact that the protein regulates the abundance of beneficial bacteria such as intestinal Akkermansia Muciniphila(Akk).All results suggest that the synthetic LPxT-GYLEQ protein may improve cognitive impairment and be a promising candidate for an anti-aging agent.展开更多
文摘Food-derived antioxidant peptides have been shown to have beneficial effects in scavenging excess free radicals.In this study,a novel multifunctional LPxTG-motif protein LPxT-GYLEQ was synthesized,and its molecular mechanism of alleviating cognitive impairment in a D-galactose(D-gal)-induced aging mice model was also investigated.The results confirmed the antioxidant effects of the LPxT-GYLEQ protein,which could scavenge excessive reactive oxygen species(ROS)in aging mice by regulating the c-Jun N-terminal kinase(JNK)/Nuclear factor erythroid 2-related factor 2(Nrf2)/p38/Nuclear factor-k-gene binding(NF-κB)signal pathway,reduced the accumulation ofβ-amyloid protein(Aβ),restored the cognitive ability of mice,improved learning and memory behavior,effectively reduced the expression of inflammatory-related factors,including tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6),and increased the expression of anti-inflammatory factor interleukin-10(IL-10).These may be related to the fact that the protein regulates the abundance of beneficial bacteria such as intestinal Akkermansia Muciniphila(Akk).All results suggest that the synthetic LPxT-GYLEQ protein may improve cognitive impairment and be a promising candidate for an anti-aging agent.
