Pasteurella multocida is a leading cause of respiratory disorders in pigs.This study was designed to understand the genotypical and antimicrobial resistant characteristics of P.multocida from pigs in China.To achieve ...Pasteurella multocida is a leading cause of respiratory disorders in pigs.This study was designed to understand the genotypical and antimicrobial resistant characteristics of P.multocida from pigs in China.To achieve this,we briefly investigated 158 P.multocida isolates from pigs with respiratory disorders in China between 2019 and 2020.Genotyping through multiplex PCR assays assigned these 158 isolates into capsular genotypes A(60.13%,95/158),D(35.44%,56/158),F(4.43%,7/158),and/or lipopolysaccharide(LPS)genotypes L3(28.48%,45/158)and L6(66.46%,105/158).In addition,eight isolates(5.06%,8/158)were found to be nontypable using the LPS genotyping method.When combining the capsular genotypes and the LPS genotypes,D:L6(34.81%,55/158)and A:L6(31.65%,50/158)were the predominant genotypes,followed by A:L3(24.05%,38/158).PCR detection of virulence factor-encoding genes showed that over 80%of the isolates were positive for exbB,tonB,exbD,ompH,ptfA,fimA,sodA,sodC,fur,ompA,oma87,plpB,hsf-2,nanH and hgbB,suggesting the presence of these genes were broad characteristics of P.multocida.We also found approximately 63.92%(101/158),51.27%(81/158),8.86%(14/158),7.59%(12/158),3.16%(5/158),0.63%(1/158),and 0.63%(1/158)of the isolates grew well in media with the presence of colistin(4μg/mL),tetracycline(16μg/mL),tigecycline(1μg/mL),ampicillin(32μg/mL),chloramphenicol(32μg/mL),cefepime(16μg/mL),and ciprofloxacin(1μg/mL),respectively.This study contributes to the understanding of genotypes and antimicrobial resistance profile of P.multocida currently circulation in pigs of China.展开更多
Background:Burkholderia pseudomallei is a gram-negative bacterium widely found in Southeast Asia and north-ern Australia.This bacterium,which lacks an available vaccine,is the causative agent of melioidosis and has pr...Background:Burkholderia pseudomallei is a gram-negative bacterium widely found in Southeast Asia and north-ern Australia.This bacterium,which lacks an available vaccine,is the causative agent of melioidosis and has properties that potentially enable its exploitation as a bioweapon.Methods:Polymerase chain reaction assays targeting each of the lipopolysaccharide(LPS)genetic types were used to investigate genotype frequencies in B.pseudomallei populations.Silver staining,gas chromatography-mass spectrometry(GC-MS),and immunofluorescence were used to characterize LPS.Results:In our study,a total of 169 clinical B.pseudomallei isolates were collected from Hainan Province,China between 2004 and 2016.The results showed that LPS genotype A was the predominant type,comprising 91.1%of the samples,compared with only 8.9%of LPS genotype B.The majority of patients were male and were diagnosed with sepsis or pneumonia.Silver staining and GC-MS demonstrated that LPS genotypes A and B exhibited distinct phenotypes and molecular structures.Immunofluorescence tests showed there was no cross-reaction between LPS genotypes A and B.Conclusions:This is the first report on the molecular epidemiology of B.pseudomallei based on O-antigen in China.Tracking the regional distribution of different LPS genotypes offers significant insights relevant to the development and administration of LPS-based vaccines.展开更多
基金This work was supported in part by the China Postdoctoral Foundation(grant 2020 T130232)the Key Laboratory of Livestock Disease Prevention of Guangdong Province and the Scientific Observation and Experiment Station of Veterinary Drugs and Diagnostic Techniques of Guangdong Province,Ministry of Agriculture and Rural Affairs,China(grant YDWS1901)Hubei Provincial Key R&D program.The funder had no role in the study design,data collection,data analysis,data interpretation,or writing of the manuscript.
文摘Pasteurella multocida is a leading cause of respiratory disorders in pigs.This study was designed to understand the genotypical and antimicrobial resistant characteristics of P.multocida from pigs in China.To achieve this,we briefly investigated 158 P.multocida isolates from pigs with respiratory disorders in China between 2019 and 2020.Genotyping through multiplex PCR assays assigned these 158 isolates into capsular genotypes A(60.13%,95/158),D(35.44%,56/158),F(4.43%,7/158),and/or lipopolysaccharide(LPS)genotypes L3(28.48%,45/158)and L6(66.46%,105/158).In addition,eight isolates(5.06%,8/158)were found to be nontypable using the LPS genotyping method.When combining the capsular genotypes and the LPS genotypes,D:L6(34.81%,55/158)and A:L6(31.65%,50/158)were the predominant genotypes,followed by A:L3(24.05%,38/158).PCR detection of virulence factor-encoding genes showed that over 80%of the isolates were positive for exbB,tonB,exbD,ompH,ptfA,fimA,sodA,sodC,fur,ompA,oma87,plpB,hsf-2,nanH and hgbB,suggesting the presence of these genes were broad characteristics of P.multocida.We also found approximately 63.92%(101/158),51.27%(81/158),8.86%(14/158),7.59%(12/158),3.16%(5/158),0.63%(1/158),and 0.63%(1/158)of the isolates grew well in media with the presence of colistin(4μg/mL),tetracycline(16μg/mL),tigecycline(1μg/mL),ampicillin(32μg/mL),chloramphenicol(32μg/mL),cefepime(16μg/mL),and ciprofloxacin(1μg/mL),respectively.This study contributes to the understanding of genotypes and antimicrobial resistance profile of P.multocida currently circulation in pigs of China.
基金supported by the National Natural Science Foundation of China(No.81901681 and No.82272350)the Science and Technology Innovation Ability Enhancement Special Project of Army Medical University(No.2019XQN04).
文摘Background:Burkholderia pseudomallei is a gram-negative bacterium widely found in Southeast Asia and north-ern Australia.This bacterium,which lacks an available vaccine,is the causative agent of melioidosis and has properties that potentially enable its exploitation as a bioweapon.Methods:Polymerase chain reaction assays targeting each of the lipopolysaccharide(LPS)genetic types were used to investigate genotype frequencies in B.pseudomallei populations.Silver staining,gas chromatography-mass spectrometry(GC-MS),and immunofluorescence were used to characterize LPS.Results:In our study,a total of 169 clinical B.pseudomallei isolates were collected from Hainan Province,China between 2004 and 2016.The results showed that LPS genotype A was the predominant type,comprising 91.1%of the samples,compared with only 8.9%of LPS genotype B.The majority of patients were male and were diagnosed with sepsis or pneumonia.Silver staining and GC-MS demonstrated that LPS genotypes A and B exhibited distinct phenotypes and molecular structures.Immunofluorescence tests showed there was no cross-reaction between LPS genotypes A and B.Conclusions:This is the first report on the molecular epidemiology of B.pseudomallei based on O-antigen in China.Tracking the regional distribution of different LPS genotypes offers significant insights relevant to the development and administration of LPS-based vaccines.
