This study reports the development of a novel and cost-effective cellulolytic enzyme cocktail,named Remzyme,using Rasamsonia emersonii.By supplementing the heterologously expressed carbohydrate-active enzymes(CAZymes)...This study reports the development of a novel and cost-effective cellulolytic enzyme cocktail,named Remzyme,using Rasamsonia emersonii.By supplementing the heterologously expressed carbohydrate-active enzymes(CAZymes)such as lytic polysaccharide monooxygenase(Rem_LPMO1,Rem_GH7CBHI),and xylanase(Malci_GH10xyl),the cocktail was optimized using a Simplex lattice mixture design.This innovative blend achieved a saccharification efficiency of 98.59%when applied to unwashed,acid/steam-pretreated rice straw slurry sourced from an industrial-scale 2G ethanol plant.The process was conducted under industrially relevant conditions with 15%substrate loading and protein loading of 8 mg/g dry substrate.Remarkably,the Remzyme cocktails was comparable to the leading commercial enzyme mix,CellicCTec3,at equivalent protein loadings,underscoring its potential as a cost-effective alternative in enzymatic saccharification.The study demonstrates the synergistic efficacy of accessory enzymes and core cellulases,offering significant advancements in enzyme technology for biorefinery applications.展开更多
文摘This study reports the development of a novel and cost-effective cellulolytic enzyme cocktail,named Remzyme,using Rasamsonia emersonii.By supplementing the heterologously expressed carbohydrate-active enzymes(CAZymes)such as lytic polysaccharide monooxygenase(Rem_LPMO1,Rem_GH7CBHI),and xylanase(Malci_GH10xyl),the cocktail was optimized using a Simplex lattice mixture design.This innovative blend achieved a saccharification efficiency of 98.59%when applied to unwashed,acid/steam-pretreated rice straw slurry sourced from an industrial-scale 2G ethanol plant.The process was conducted under industrially relevant conditions with 15%substrate loading and protein loading of 8 mg/g dry substrate.Remarkably,the Remzyme cocktails was comparable to the leading commercial enzyme mix,CellicCTec3,at equivalent protein loadings,underscoring its potential as a cost-effective alternative in enzymatic saccharification.The study demonstrates the synergistic efficacy of accessory enzymes and core cellulases,offering significant advancements in enzyme technology for biorefinery applications.
