Objective To study the mechanism of lactose intolerance （LI） by cloning the mouse lactase cDNA and recombining a vector. Methods Total murine RNA was isolated from the small intestine of a 4-week-old BALB/c mouse ...Objective To study the mechanism of lactose intolerance （LI） by cloning the mouse lactase cDNA and recombining a vector. Methods Total murine RNA was isolated from the small intestine of a 4-week-old BALB/c mouse （δ）. Crene-specific primers were designed and synthesized according to the cDNA sequences of lactase-phlorizin hydrolase （LPH） in human, rat, and rabbit. A coding sequence （CDS） fragment was obtained using RT-PCR, and inserted into a clone vector pNEB-193, then the cDNA was sequenced and analyzed using bioinformatics. Results The cDNA from the BALB/c mouse with 912 bp encoding 303 amino acid residues. Analysis of the deduced amino acid sequence using bioinforrnatics revealed that this cDNA shared extensive sequence homology with human LPH containing a conserved glycosyl hydrolase family 1 motif important for regulating lactase intolerance. Conclusion BALB/c mouse LPH cDNA （GenBank accession No： AY751548） provides a necessary foundation for study of the biological function and regulatory mechanism of the lactose intolerance in mice.展开更多
There are various applied electro-optical devices, which utilize light emitting didoe(LED) chip array for applications to displays and opto-electronic sensors. In those devices, it is the one of the critical technical...There are various applied electro-optical devices, which utilize light emitting didoe(LED) chip array for applications to displays and opto-electronic sensors. In those devices, it is the one of the critical technical issues to minimize uncertain fluctuations including optical power and optical density. Due to variation in operating environment of a device, those are not corrected precisely by controlling parameters based on simple relation between parameters and resultant abovementioned outputs.Therefore, there is essential need to correct outputs in real-time based on correction function generated from the consideration on various operation condition. In this article, we introduce an output correction method through reporting real-time image noise reduction in the application to electro-photography with LED print head. In the technology of LED print head, as differences in optical characteristics between each LED cause vertical image noise, it should be corrected in order to obtain images that are comparable or better in quality compared to those produced by the conventional laser scanning method. Even though it seems that the method used to obtain uniform light power from each LED can solve this problem, it does not work well for high-resolution printing. Therefore, a scan method involving correction by a printed and scanned pattern is introduced through this work. The scan method is composed of correction patterns to minimize printing noise by its shape, the correction algorithm to calculate the optimized value and the printing algorithm to control gray levels in real-time precisely. We believe that the developed correction method upgrades the printing quality of the LPH printer better than commercial printers. The developed correction method can also be applied to various application areas that use an array-type light source such as display systems and lighting systems.展开更多
基金This work was supported by National Natural Science Foundation China (No. 30271126).
文摘Objective To study the mechanism of lactose intolerance （LI） by cloning the mouse lactase cDNA and recombining a vector. Methods Total murine RNA was isolated from the small intestine of a 4-week-old BALB/c mouse （δ）. Crene-specific primers were designed and synthesized according to the cDNA sequences of lactase-phlorizin hydrolase （LPH） in human, rat, and rabbit. A coding sequence （CDS） fragment was obtained using RT-PCR, and inserted into a clone vector pNEB-193, then the cDNA was sequenced and analyzed using bioinformatics. Results The cDNA from the BALB/c mouse with 912 bp encoding 303 amino acid residues. Analysis of the deduced amino acid sequence using bioinforrnatics revealed that this cDNA shared extensive sequence homology with human LPH containing a conserved glycosyl hydrolase family 1 motif important for regulating lactase intolerance. Conclusion BALB/c mouse LPH cDNA （GenBank accession No： AY751548） provides a necessary foundation for study of the biological function and regulatory mechanism of the lactose intolerance in mice.
基金supported by the National Research Foundation of Korea Grant funded by the Korean Government(Grant No.2015R1C1A1A01053888)the Yeungnam University Research Grant(Grant No.216A580022)
文摘There are various applied electro-optical devices, which utilize light emitting didoe(LED) chip array for applications to displays and opto-electronic sensors. In those devices, it is the one of the critical technical issues to minimize uncertain fluctuations including optical power and optical density. Due to variation in operating environment of a device, those are not corrected precisely by controlling parameters based on simple relation between parameters and resultant abovementioned outputs.Therefore, there is essential need to correct outputs in real-time based on correction function generated from the consideration on various operation condition. In this article, we introduce an output correction method through reporting real-time image noise reduction in the application to electro-photography with LED print head. In the technology of LED print head, as differences in optical characteristics between each LED cause vertical image noise, it should be corrected in order to obtain images that are comparable or better in quality compared to those produced by the conventional laser scanning method. Even though it seems that the method used to obtain uniform light power from each LED can solve this problem, it does not work well for high-resolution printing. Therefore, a scan method involving correction by a printed and scanned pattern is introduced through this work. The scan method is composed of correction patterns to minimize printing noise by its shape, the correction algorithm to calculate the optimized value and the printing algorithm to control gray levels in real-time precisely. We believe that the developed correction method upgrades the printing quality of the LPH printer better than commercial printers. The developed correction method can also be applied to various application areas that use an array-type light source such as display systems and lighting systems.
