HPLC fingerprinting and quantification of gentiopicroside（GPS） and loganic acid（LA） in Gentianae Macrophyllae Radix（GMR） crude drugs were developed in this study.The samples were separated on Zorbax SB-C_（18） ...HPLC fingerprinting and quantification of gentiopicroside（GPS） and loganic acid（LA） in Gentianae Macrophyllae Radix（GMR） crude drugs were developed in this study.The samples were separated on Zorbax SB-C_（18） column（250 mm×4.6 mm, 5μm） with a linear gradient of acetonitrile and 0.04%phosphoric acid.The HPLC flow rate was 1.0 mL/min and a UV absorption was measured at 230 nm.An orthogonal L9（3^4） test was applied for the optimization of sample extraction conditions,and an aliquot of GMR sample（g） was extracted with 15-fold of 50%ethanol（mL） for 30 min by sonication.Quantitative analysis showed that the content of GPS（14.05 mg/g-74.61 mg/g） in all samples was obviously higher than that of LA（1.13 mg/g-40.46 mg/g）. Based on the content ratio of GPS over LA（1.8-11.4）,samples originated from Gentiana macrophylla（with content ratio of GPS over LA≤4.3） could be distinguished from those from G.dahurica and G.dahurica var.gracilipes（with content ratio of GPS over LA≥4.8）.The principle components analysis of the HPLC fingerprints showed that samples originated from G.macrophylla and G.dahurica（including G.dahurica var.gracilipes） could be divided into two groups.This established HPLC-DAD method could be efficiently used for the species identification and quality control of GMR crude drugs.展开更多
mmJ这个骷髅头，不知不觉已经走过了10个年头。
07年下半年，正是本间正章发动mastermind JAPAN 10周年企画的大日子，「THANKYOU」FW系列更说明是贯穿整个10周年企画的别注系列。顾名思义，mmJ除了mainline的10周年外，本间更联同一...mmJ这个骷髅头，不知不觉已经走过了10个年头。
07年下半年，正是本间正章发动mastermind JAPAN 10周年企画的大日子，「THANKYOU」FW系列更说明是贯穿整个10周年企画的别注系列。顾名思义，mmJ除了mainline的10周年外，本间更联同一众好友品牌推出10周年限定别注，中村世纪的visvim是就是其合作的重头之一。展开更多
基金Research Program of Science Technology of Traditional Chinese Medicine(TCM) Sponsored by the State Administration of TCM of China(Grant No.04-05ZL01)
文摘HPLC fingerprinting and quantification of gentiopicroside（GPS） and loganic acid（LA） in Gentianae Macrophyllae Radix（GMR） crude drugs were developed in this study.The samples were separated on Zorbax SB-C_（18） column（250 mm×4.6 mm, 5μm） with a linear gradient of acetonitrile and 0.04%phosphoric acid.The HPLC flow rate was 1.0 mL/min and a UV absorption was measured at 230 nm.An orthogonal L9（3^4） test was applied for the optimization of sample extraction conditions,and an aliquot of GMR sample（g） was extracted with 15-fold of 50%ethanol（mL） for 30 min by sonication.Quantitative analysis showed that the content of GPS（14.05 mg/g-74.61 mg/g） in all samples was obviously higher than that of LA（1.13 mg/g-40.46 mg/g）. Based on the content ratio of GPS over LA（1.8-11.4）,samples originated from Gentiana macrophylla（with content ratio of GPS over LA≤4.3） could be distinguished from those from G.dahurica and G.dahurica var.gracilipes（with content ratio of GPS over LA≥4.8）.The principle components analysis of the HPLC fingerprints showed that samples originated from G.macrophylla and G.dahurica（including G.dahurica var.gracilipes） could be divided into two groups.This established HPLC-DAD method could be efficiently used for the species identification and quality control of GMR crude drugs.
文摘mmJ这个骷髅头，不知不觉已经走过了10个年头。
07年下半年，正是本间正章发动mastermind JAPAN 10周年企画的大日子，「THANKYOU」FW系列更说明是贯穿整个10周年企画的别注系列。顾名思义，mmJ除了mainline的10周年外，本间更联同一众好友品牌推出10周年限定别注，中村世纪的visvim是就是其合作的重头之一。
