Objective:To investigate the effect of Catalpa ovata fruit extract(COFE)on muscle growth and exercise performance in C2C12 myoblasts and mice.Me t h o d s:Cel l viabi l i ty was determined through a 3-(4,5-dimethylthi...Objective:To investigate the effect of Catalpa ovata fruit extract(COFE)on muscle growth and exercise performance in C2C12 myoblasts and mice.Me t h o d s:Cel l viabi l i ty was determined through a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.Myogenic differentiation was observed using Giemsa staining.COFE was administered to mice orally at 50 and 200 mg/kg for 10 weeks.Muscular strength was evaluated using the whole-limb grip strength assay.The expression levels of myogenesis-and energy metabolism-related proteins in vitro and in vivo were determined using Western blotting.Results:COFE significantly improved myoblast-to-myotube differentiation in C2C12 myoblasts.It also increased the expression of myogenesis determination protein 1 and myogenin compared with the control group.Moreover,the expression levels of glucose transporter type 4(Glut4)and peroxisome proliferator-activated receptor-gamma coactivator 1-alpha(PGC-1α)were significantly elevated in the presence of COFE in C2C12 myoblasts.COFE also markedly increased phosphorylation of AMP-activated protein kinase,which regulates Glut4 and PGC-1αexpression levels in C2C12 myoblasts.Mice treated with COFE showed improved grip strength.Myogenesis-and energy metabolism-related protein levels in muscle tissue were significantly increased in COFE-administered mice.Conclusions:COFE treatment improves exercise performance by controlling myogenesis and energy metabolism in skeletal muscle.COFE has the potential to be used as an effective natural agent for enhancing muscular strength.展开更多
Locust plagues result in significant agricultural and ecological damage,and the current dependence on broadspectrum chemical pesticides raises serious concerns regarding environmental sustainability and public health....Locust plagues result in significant agricultural and ecological damage,and the current dependence on broadspectrum chemical pesticides raises serious concerns regarding environmental sustainability and public health.In this study,we elucidated the biosynthetic pathway of the locust aggregation pheromone 4-vinylanisole(4VA).Through analysis of the crystal structure of the 4VPMT2-4VA-SAM complex,it was determined that 4-nitrophenol acts as a substrate analogue,effectively inhibiting 4VPMT enzyme activity and thereby preventing the formation of 4VA.This study revealed key enzymatic targets and lead inhibitors for intervention,establishing a molecular foundation for pheromone-based,environmentally sustainable locust control strategies and offering a viable alternative to reduce reliance on conventional chemical pesticides.展开更多
Catalpa bungei,a fast-growing timber tree,is threatened by the lepidopteran pest Omphisa plagialis.Previous studies in our laboratory successfully generated transgenic C.bungei lines overexpressing Cry genes(Cry1Ab,Cr...Catalpa bungei,a fast-growing timber tree,is threatened by the lepidopteran pest Omphisa plagialis.Previous studies in our laboratory successfully generated transgenic C.bungei lines overexpressing Cry genes(Cry1Ab,Cry2A,and Cry9-2)that exhibited resistance to O.plagialis,but their potential impact on soil bacterial communities remains unclear.In this study,we analyzed nine transgenic C.bungei lines(three independent lines for each Cry gene)to characterize their rhizosphere bacterial communities using high-throughput sequencing of the 16S ribosomal DNA(rDNA)V4-V5 regions.A total of 628 amplicon sequence variants(ASVs)were shared among all transgenic and wild-type(WT)lines,forming a stable core microbiome dominated by Proteobacteria,Bacteroidota,Acidobacteriota,and Actinobacteriota.Alpha diversity showed no significant differences,while beta diversity revealed minor but distinct compositional shifts.Cry1Ab lines exhibited higher abundances of fast-growing taxa,particularly Proteobacteria and Bacteroidota;Cry2A lines displayed intermediate profiles,whereas Cry9-2 lines were nearly indistinguishable from WT communities.Linear discriminant analysis of the effect size revealed significant enrichment of taxa such as Burkholderiaceae and Ralstonia in the Cry1Ab rhizosphere,in contrast to the higher abundance of Chloroflexi in the WT.Functional predictions indicated consistent metabolic pathways across all treatments,suggesting strong ecological redundancy.This study demonstrates minimal impact on rhizosphere microbial communities in transgenic C.bungei plants.The Cry9-2 construct exhibited superior environmental stability,whereas the Cry1Ab construct caused only slight but ecologically acceptable shifts.These findings support the ecological safety of Bt-transgenic C.bungei and identify Cry9-2 as a particularly favorable candidate for forestry applications.This comparative evaluation of three Cry genes in a tree species provides a framework for future gene-specific biosafety assessments in woody plants.展开更多
空泡份额是核反应堆两相流中关注的重点参数之一,是检验压水堆系统级程序计算能力的重要物理量。为加速我国压水堆软件自主化进程,中国广核集团有限公司开发了压水堆热工水力系统分析软件LOCUST。为支持LOCUST在冷却剂丧失事故(Loss of ...空泡份额是核反应堆两相流中关注的重点参数之一,是检验压水堆系统级程序计算能力的重要物理量。为加速我国压水堆软件自主化进程,中国广核集团有限公司开发了压水堆热工水力系统分析软件LOCUST。为支持LOCUST在冷却剂丧失事故(Loss of Coolant Accident,LOCA)等事故分析中的应用,本文基于瑞典通用电机公司开展的FRIGG空泡份额实验,利用LOCUST对不同功率分布、入口过冷度以及质量流速的实验工况进行计算验证。评估结果表明,所有工况空泡份额相对误差的平均值为10.60%,单个工况最大相对误差的平均值不超过16.62%,部分误差可能是由流型转换判定、高估液相相变换热等原因引起。本文总体验证了LOCUST在不同功率分布、入口过冷度以及质量流速工况下对空泡份额的计算能力,为后续软件模型改进提供了参考。展开更多
Tropidacris spp. represent the largest known group among acridoids. Their presence ranges from southeastern Mexico and has so far been confirmed only in tropical habitats. This publication seeks to highlight the curre...Tropidacris spp. represent the largest known group among acridoids. Their presence ranges from southeastern Mexico and has so far been confirmed only in tropical habitats. This publication seeks to highlight the current and potential challenges associated with their presence. For decades, the use of chemical insecticides has been the primary method for controlling locust populations, though these substances pose significant risks to human health and the environment. Recent research efforts are directed toward developing control methods that are less detrimental to both ecological and human health, such as biopesticides derived from pathogenic fungi, plant extracts, and strategically prescribed burns. Satellite surveillance enables the monitoring of the origination and progression of outbreaks to inform control strategy selection.展开更多
The ability of cyborg locusts to achieve directional movement in complex outdoor environments is critical for search and rescue missions.Currently,there is a lack of research on motion control for cyborg locusts in ou...The ability of cyborg locusts to achieve directional movement in complex outdoor environments is critical for search and rescue missions.Currently,there is a lack of research on motion control for cyborg locusts in outdoor settings.In this study,we developed cyborg locusts capable of performing directional locomotion in intricate outdoor environments,including jumping over obstacles,climbing slopes,traversing narrow pipelines,and accurately reaching predetermined targets along specified routes.We designed a miniature electrical backpack(10 mm×10 mm,0.75 g)capable of receiving stimulus parameters(frequency,duty ratio,and stimulation time)via Bluetooth commands from mobile phones.Electrical stimulation of locust sensory organs,such as the antennae and cercus,induced turning and jumping behaviors.Experi-mental testing of locust movement control was conducted under outdoor conditions with a short electrical stimulation interval.Results showed a positive correlation between locust turning angles and electrical stimulation parameters within a specified range,with an average jumping height exceeding 10 cm.Additionally,the success rate of locust turning and jumping behaviors correlated positively with the interval time between electrical stimulations.Adjusting these intervals during forward crawling phases increased the likelihood of the locusts jumping again.In conclusion,this study success-fully achieved directional locomotion control of cyborg locusts outdoors,providing insights and references for advancing search and rescue capabilities.展开更多
The mechanism of plant response to water availability is a crucial research area in ecosystem adaptation to dry environments.Global change in precipitation patterns exacerbates drought risks in many regions,necessitat...The mechanism of plant response to water availability is a crucial research area in ecosystem adaptation to dry environments.Global change in precipitation patterns exacerbates drought risks in many regions,necessitating investigations of tree responses to drought.However,compared with seasonal drought events,how water use characteristics respond to long-term rainfall reduction remains poorly characterized.Here,we assessed the effects of multi-year rainfall exclusion treatments(in the 4th and 5th years)on sap flow,canopy conductance(G_(C)),and the response pattern to varying transpiration driving force(vapor pressure deficit(VPD))and soil moisture conditions in a black locust(Robinia pseudoacacia)plantation in the China's Loess Plateau.The experimental platform includes paired plots of a 30%throughfall exclusion treatment and a control within the plantation.The reduction of soil water content(SWC),either as temporal variations or as a result of rainfall exclusion treatment,generally lowered sap flow,G_(C),and the sensitivity to VPD.The difference in each index between plots was substantially greater than that among temporal variations in SWC within a plot.The trees in the control plot showed strong responses of G_(C)to temporal SWC changes,exhibiting an isohydric behavior.However,those subjected to long-term rainfall exclusion treatment had lower variations in G_(C)and the sensitivity across different SWC,exhibiting an anisohydric behavior under limited water conditions.Additionally,rainfall exclusion treatment decreased basal area(BA)increment but increased water use efficiency(WUE).The transition to anisohydric behavior suggests an adventurous pattern of water use strategy that has risks of hydraulic damage and shoot mortality.These findings provide insights into the different adaptation strategies within a species to diverse intensity and duration of drought conditions,which is crucial for sustainable forest management under climate change conditions.展开更多
[Objective] This study aimed to investigate the effects of drought stress on osmotic regulation substances of Catalpa bungei clones. [Method] Potting experimental was carried out with plastic film sealing method, to d...[Objective] This study aimed to investigate the effects of drought stress on osmotic regulation substances of Catalpa bungei clones. [Method] Potting experimental was carried out with plastic film sealing method, to differentiate the degrees of drought stress based on changes in soil water content and leaf water potential and investigate variations in contents of osmotic regulation substances（free proline,soluble sugar and soluble protein） in leaves of five Catalpa bungei clones under different degrees of drought stresses. [Result] According to changes in soil water content and leaf water potential, the whole process of drought stress was divided into normal level（CK, with soil water content of 97.49% and leaf water potential of-0.54MPa）, light drought（LD with soil water content of 59.96% and leaf water potential of-1.28 MPa）, mediate drought（MD with soil water content of 34.19% and leaf water potential of-2.32 MPa） and severe drought（SD with soil water content of 14.52%and leaf water potential of-2.99 MPa）. The soil water content and leaf water potential of five Catalpa bungei clones reached the highest correlation in exponential fitting, with an average R2of 0.989 3（P0.001）. The free proline content in leaves of five Catalpa bungei clones increased rapidly with the increasing degree of drought stress（P 0.001）. To be specific, free proline contents of Catalpa bungei clones015-1 and 7080 were 34.39 and 33.41 times of the normal level under severe drought conditions, which reached an extremely significant level（P0.001）; the free proline content of Catalpa bungei clone 1-3 rapidly increased to（855.46±227.52） μg/g Fw under light drought conditions. The soluble protein content in leaves showed different variation trends. To be specific, the soluble protein content of Catalpa bungei clone 7080 was the lowest at various drought stages and reached（1.644 ±0.137）mg/g Fw under normal conditions; the soluble protein content of Catalpa bungei clone 1-3 was relatively high under normal conditions and was reduced rapidly under light drought conditions, showing different response patterns. [Conclusion] Osmotic regulation substances in leaves of five Catalpa bungei clones all have certain response to drought stress. Free proline is the most important osmotic regulation substance, followed by soluble protein, while soluble sugar makes no significant contribution. According to changes in content of osmotic regulation substances, Catalpa bungei clone 7080 has relatively high capacity and good drought resistance, while Catalpa bungei clone 1-3 has the rapidest response to drought stress.展开更多
To know the annual water consumption of forest, it is necessary to acquire the annual transpiration value of stands. This paper is based on the data measured in the typical weather of the growth season, from 1998 to 2...To know the annual water consumption of forest, it is necessary to acquire the annual transpiration value of stands. This paper is based on the data measured in the typical weather of the growth season, from 1998 to 2000, with the LI 1600 Steady Porometer and the general weather information. The daily variation of transpiration in black locust forest ( Robinia pesudoacacia L.) is modeled by Penman Monteith equation. As a result of the model, a continuous daily transpiration in the growth season was calculated. The net radiation, intercepted by black locust forest canopy, was acquired from a semi empirical equation of measuring net radiation R n with the extinction coefficient k and leaf area index LAI . The canopy integral stomatic resistance is a mimesis with an empirical equation of measuring data. Compared with measuring data, the relative error of the modeled ones is less than 12% averagely. At last, the total transpiration of black locust forest during the period of 1998 and 2000 in the growth season of May to October, as an average transpiration of the different density stands, were 192 46, 187 07 and 195 59?mm respectively.展开更多
[Objective] This study aimed to provide scientific basis for the early selec-tion and breeding of excellent Catalpa bungei clones. [Method] The Li-6400 portable photosynthesis system was used to analyze the photosynth...[Objective] This study aimed to provide scientific basis for the early selec-tion and breeding of excellent Catalpa bungei clones. [Method] The Li-6400 portable photosynthesis system was used to analyze the photosynthetic characteristics of five Catalpa bungei clones （1-3, 1-4, 004-1, 015-1 and 7080）. [Result] Al five Catalpa bungei clones had similar change trends of light response curves, but their maxi-mum net photosynthetic rates （Pmax） were in the order of 1-3＆gt;7080＆gt;015-1＆gt;004-1＆gt;1-4, showing that clone 1-3 and clone 7080 had strong light environmental adapt-ability with the light saturation points （LSP） of 1 147.97 μmol/m2＆#183;s and 1 196.31μmol/m2＆#183;s, respectively, while clone 1-4 had the narrowest range of adaptation to light. There were differences in the total photosynthetic amount per area among the five clones that clone 1-4 was the best of 433 974.24 μmol/m2, fol owed by clone 1-3 of 401 290.20 μmol/m2. The transpiration rates （Tr） and stomatal conductances （Cond） of the five clones presented the same change trend. And the diurnal varia-tion curves of net photosynthetic rate （Pn） of clone 1-3, 1-4 and 015-1 showed two typical peaks with obvious midday depression, while clone 004-1 and 7080 didn’t. [Concluson] Considering a variety of photosynthetic characteristics of indica-tors, photosynthetic capacity of clone 1-3 was the best among the five Catalpa bungei clones.展开更多
基金supported by Project to Make Multi-Ministerial National Biological Research Resources More Advanced Program,Korea Environment Industry&Technology Institute,and funded by Korea Ministry of Environment(grant number RS-2023-00230403).
文摘Objective:To investigate the effect of Catalpa ovata fruit extract(COFE)on muscle growth and exercise performance in C2C12 myoblasts and mice.Me t h o d s:Cel l viabi l i ty was determined through a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.Myogenic differentiation was observed using Giemsa staining.COFE was administered to mice orally at 50 and 200 mg/kg for 10 weeks.Muscular strength was evaluated using the whole-limb grip strength assay.The expression levels of myogenesis-and energy metabolism-related proteins in vitro and in vivo were determined using Western blotting.Results:COFE significantly improved myoblast-to-myotube differentiation in C2C12 myoblasts.It also increased the expression of myogenesis determination protein 1 and myogenin compared with the control group.Moreover,the expression levels of glucose transporter type 4(Glut4)and peroxisome proliferator-activated receptor-gamma coactivator 1-alpha(PGC-1α)were significantly elevated in the presence of COFE in C2C12 myoblasts.COFE also markedly increased phosphorylation of AMP-activated protein kinase,which regulates Glut4 and PGC-1αexpression levels in C2C12 myoblasts.Mice treated with COFE showed improved grip strength.Myogenesis-and energy metabolism-related protein levels in muscle tissue were significantly increased in COFE-administered mice.Conclusions:COFE treatment improves exercise performance by controlling myogenesis and energy metabolism in skeletal muscle.COFE has the potential to be used as an effective natural agent for enhancing muscular strength.
基金support of the National Natural Science Foundation of China(32472594).
文摘Locust plagues result in significant agricultural and ecological damage,and the current dependence on broadspectrum chemical pesticides raises serious concerns regarding environmental sustainability and public health.In this study,we elucidated the biosynthetic pathway of the locust aggregation pheromone 4-vinylanisole(4VA).Through analysis of the crystal structure of the 4VPMT2-4VA-SAM complex,it was determined that 4-nitrophenol acts as a substrate analogue,effectively inhibiting 4VPMT enzyme activity and thereby preventing the formation of 4VA.This study revealed key enzymatic targets and lead inhibitors for intervention,establishing a molecular foundation for pheromone-based,environmentally sustainable locust control strategies and offering a viable alternative to reduce reliance on conventional chemical pesticides.
基金funded by the Chinese Academy of Forestry-Special funds for basic scientific research service expenses of the central level public welfare research institutes(Grant No.CAFYBB2020QD001)the National Natural Science Foundation of China(Grant Nos.32101550,32271917)+1 种基金Jiangsu Agricultural Science and Technology Innovation Fund(Grant No.CX(24)3052)National Forestry and Grassland Administration’s Center for Science and Technology Development Projects(Grant No.KJZXSA202202).
文摘Catalpa bungei,a fast-growing timber tree,is threatened by the lepidopteran pest Omphisa plagialis.Previous studies in our laboratory successfully generated transgenic C.bungei lines overexpressing Cry genes(Cry1Ab,Cry2A,and Cry9-2)that exhibited resistance to O.plagialis,but their potential impact on soil bacterial communities remains unclear.In this study,we analyzed nine transgenic C.bungei lines(three independent lines for each Cry gene)to characterize their rhizosphere bacterial communities using high-throughput sequencing of the 16S ribosomal DNA(rDNA)V4-V5 regions.A total of 628 amplicon sequence variants(ASVs)were shared among all transgenic and wild-type(WT)lines,forming a stable core microbiome dominated by Proteobacteria,Bacteroidota,Acidobacteriota,and Actinobacteriota.Alpha diversity showed no significant differences,while beta diversity revealed minor but distinct compositional shifts.Cry1Ab lines exhibited higher abundances of fast-growing taxa,particularly Proteobacteria and Bacteroidota;Cry2A lines displayed intermediate profiles,whereas Cry9-2 lines were nearly indistinguishable from WT communities.Linear discriminant analysis of the effect size revealed significant enrichment of taxa such as Burkholderiaceae and Ralstonia in the Cry1Ab rhizosphere,in contrast to the higher abundance of Chloroflexi in the WT.Functional predictions indicated consistent metabolic pathways across all treatments,suggesting strong ecological redundancy.This study demonstrates minimal impact on rhizosphere microbial communities in transgenic C.bungei plants.The Cry9-2 construct exhibited superior environmental stability,whereas the Cry1Ab construct caused only slight but ecologically acceptable shifts.These findings support the ecological safety of Bt-transgenic C.bungei and identify Cry9-2 as a particularly favorable candidate for forestry applications.This comparative evaluation of three Cry genes in a tree species provides a framework for future gene-specific biosafety assessments in woody plants.
文摘空泡份额是核反应堆两相流中关注的重点参数之一,是检验压水堆系统级程序计算能力的重要物理量。为加速我国压水堆软件自主化进程,中国广核集团有限公司开发了压水堆热工水力系统分析软件LOCUST。为支持LOCUST在冷却剂丧失事故(Loss of Coolant Accident,LOCA)等事故分析中的应用,本文基于瑞典通用电机公司开展的FRIGG空泡份额实验,利用LOCUST对不同功率分布、入口过冷度以及质量流速的实验工况进行计算验证。评估结果表明,所有工况空泡份额相对误差的平均值为10.60%,单个工况最大相对误差的平均值不超过16.62%,部分误差可能是由流型转换判定、高估液相相变换热等原因引起。本文总体验证了LOCUST在不同功率分布、入口过冷度以及质量流速工况下对空泡份额的计算能力,为后续软件模型改进提供了参考。
文摘Tropidacris spp. represent the largest known group among acridoids. Their presence ranges from southeastern Mexico and has so far been confirmed only in tropical habitats. This publication seeks to highlight the current and potential challenges associated with their presence. For decades, the use of chemical insecticides has been the primary method for controlling locust populations, though these substances pose significant risks to human health and the environment. Recent research efforts are directed toward developing control methods that are less detrimental to both ecological and human health, such as biopesticides derived from pathogenic fungi, plant extracts, and strategically prescribed burns. Satellite surveillance enables the monitoring of the origination and progression of outbreaks to inform control strategy selection.
基金supported by Postgraduate Research&Practice Innovation Program of Jiangsu Province under Grant KYCX22_0290.
文摘The ability of cyborg locusts to achieve directional movement in complex outdoor environments is critical for search and rescue missions.Currently,there is a lack of research on motion control for cyborg locusts in outdoor settings.In this study,we developed cyborg locusts capable of performing directional locomotion in intricate outdoor environments,including jumping over obstacles,climbing slopes,traversing narrow pipelines,and accurately reaching predetermined targets along specified routes.We designed a miniature electrical backpack(10 mm×10 mm,0.75 g)capable of receiving stimulus parameters(frequency,duty ratio,and stimulation time)via Bluetooth commands from mobile phones.Electrical stimulation of locust sensory organs,such as the antennae and cercus,induced turning and jumping behaviors.Experi-mental testing of locust movement control was conducted under outdoor conditions with a short electrical stimulation interval.Results showed a positive correlation between locust turning angles and electrical stimulation parameters within a specified range,with an average jumping height exceeding 10 cm.Additionally,the success rate of locust turning and jumping behaviors correlated positively with the interval time between electrical stimulations.Adjusting these intervals during forward crawling phases increased the likelihood of the locusts jumping again.In conclusion,this study success-fully achieved directional locomotion control of cyborg locusts outdoors,providing insights and references for advancing search and rescue capabilities.
基金supported by the National Key R&D Program of China(No.2017YFC0504601)an open fund of the State Key Laboratory of Soil and Water Conservation and Desertification Control(No.F2010121002-202424).
文摘The mechanism of plant response to water availability is a crucial research area in ecosystem adaptation to dry environments.Global change in precipitation patterns exacerbates drought risks in many regions,necessitating investigations of tree responses to drought.However,compared with seasonal drought events,how water use characteristics respond to long-term rainfall reduction remains poorly characterized.Here,we assessed the effects of multi-year rainfall exclusion treatments(in the 4th and 5th years)on sap flow,canopy conductance(G_(C)),and the response pattern to varying transpiration driving force(vapor pressure deficit(VPD))and soil moisture conditions in a black locust(Robinia pseudoacacia)plantation in the China's Loess Plateau.The experimental platform includes paired plots of a 30%throughfall exclusion treatment and a control within the plantation.The reduction of soil water content(SWC),either as temporal variations or as a result of rainfall exclusion treatment,generally lowered sap flow,G_(C),and the sensitivity to VPD.The difference in each index between plots was substantially greater than that among temporal variations in SWC within a plot.The trees in the control plot showed strong responses of G_(C)to temporal SWC changes,exhibiting an isohydric behavior.However,those subjected to long-term rainfall exclusion treatment had lower variations in G_(C)and the sensitivity across different SWC,exhibiting an anisohydric behavior under limited water conditions.Additionally,rainfall exclusion treatment decreased basal area(BA)increment but increased water use efficiency(WUE).The transition to anisohydric behavior suggests an adventurous pattern of water use strategy that has risks of hydraulic damage and shoot mortality.These findings provide insights into the different adaptation strategies within a species to diverse intensity and duration of drought conditions,which is crucial for sustainable forest management under climate change conditions.
基金Supported by National Scientific and Technological Project in Rural Areas in the "Twelfth Five Year" Plan(2012BAD21B03,2012BAD21B0304)~~
文摘[Objective] This study aimed to investigate the effects of drought stress on osmotic regulation substances of Catalpa bungei clones. [Method] Potting experimental was carried out with plastic film sealing method, to differentiate the degrees of drought stress based on changes in soil water content and leaf water potential and investigate variations in contents of osmotic regulation substances（free proline,soluble sugar and soluble protein） in leaves of five Catalpa bungei clones under different degrees of drought stresses. [Result] According to changes in soil water content and leaf water potential, the whole process of drought stress was divided into normal level（CK, with soil water content of 97.49% and leaf water potential of-0.54MPa）, light drought（LD with soil water content of 59.96% and leaf water potential of-1.28 MPa）, mediate drought（MD with soil water content of 34.19% and leaf water potential of-2.32 MPa） and severe drought（SD with soil water content of 14.52%and leaf water potential of-2.99 MPa）. The soil water content and leaf water potential of five Catalpa bungei clones reached the highest correlation in exponential fitting, with an average R2of 0.989 3（P0.001）. The free proline content in leaves of five Catalpa bungei clones increased rapidly with the increasing degree of drought stress（P 0.001）. To be specific, free proline contents of Catalpa bungei clones015-1 and 7080 were 34.39 and 33.41 times of the normal level under severe drought conditions, which reached an extremely significant level（P0.001）; the free proline content of Catalpa bungei clone 1-3 rapidly increased to（855.46±227.52） μg/g Fw under light drought conditions. The soluble protein content in leaves showed different variation trends. To be specific, the soluble protein content of Catalpa bungei clone 7080 was the lowest at various drought stages and reached（1.644 ±0.137）mg/g Fw under normal conditions; the soluble protein content of Catalpa bungei clone 1-3 was relatively high under normal conditions and was reduced rapidly under light drought conditions, showing different response patterns. [Conclusion] Osmotic regulation substances in leaves of five Catalpa bungei clones all have certain response to drought stress. Free proline is the most important osmotic regulation substance, followed by soluble protein, while soluble sugar makes no significant contribution. According to changes in content of osmotic regulation substances, Catalpa bungei clone 7080 has relatively high capacity and good drought resistance, while Catalpa bungei clone 1-3 has the rapidest response to drought stress.
基金SupportedbytheNationalNaturalScienceFoundationofChina(39970 6 2 2 )andtheTeachingandResearchAwardProgramforYongCadremanTeachersinHigherEducationInstitutionsofMOE P .R .China
文摘To know the annual water consumption of forest, it is necessary to acquire the annual transpiration value of stands. This paper is based on the data measured in the typical weather of the growth season, from 1998 to 2000, with the LI 1600 Steady Porometer and the general weather information. The daily variation of transpiration in black locust forest ( Robinia pesudoacacia L.) is modeled by Penman Monteith equation. As a result of the model, a continuous daily transpiration in the growth season was calculated. The net radiation, intercepted by black locust forest canopy, was acquired from a semi empirical equation of measuring net radiation R n with the extinction coefficient k and leaf area index LAI . The canopy integral stomatic resistance is a mimesis with an empirical equation of measuring data. Compared with measuring data, the relative error of the modeled ones is less than 12% averagely. At last, the total transpiration of black locust forest during the period of 1998 and 2000 in the growth season of May to October, as an average transpiration of the different density stands, were 192 46, 187 07 and 195 59?mm respectively.
基金Supported by the National Key Technology R&D Program(2012BAD21B03)Specialized Research Fund for The Doctoral Program of Higher Education(20124404120008)~~
文摘[Objective] This study aimed to provide scientific basis for the early selec-tion and breeding of excellent Catalpa bungei clones. [Method] The Li-6400 portable photosynthesis system was used to analyze the photosynthetic characteristics of five Catalpa bungei clones （1-3, 1-4, 004-1, 015-1 and 7080）. [Result] Al five Catalpa bungei clones had similar change trends of light response curves, but their maxi-mum net photosynthetic rates （Pmax） were in the order of 1-3＆gt;7080＆gt;015-1＆gt;004-1＆gt;1-4, showing that clone 1-3 and clone 7080 had strong light environmental adapt-ability with the light saturation points （LSP） of 1 147.97 μmol/m2＆#183;s and 1 196.31μmol/m2＆#183;s, respectively, while clone 1-4 had the narrowest range of adaptation to light. There were differences in the total photosynthetic amount per area among the five clones that clone 1-4 was the best of 433 974.24 μmol/m2, fol owed by clone 1-3 of 401 290.20 μmol/m2. The transpiration rates （Tr） and stomatal conductances （Cond） of the five clones presented the same change trend. And the diurnal varia-tion curves of net photosynthetic rate （Pn） of clone 1-3, 1-4 and 015-1 showed two typical peaks with obvious midday depression, while clone 004-1 and 7080 didn’t. [Concluson] Considering a variety of photosynthetic characteristics of indica-tors, photosynthetic capacity of clone 1-3 was the best among the five Catalpa bungei clones.
