Softening of fleshy fruits during ripening and postharvest is a programmed physiological process that substantially impacts fruit quality and shelf life.However,the molecular mechanism underlying peach softening remai...Softening of fleshy fruits during ripening and postharvest is a programmed physiological process that substantially impacts fruit quality and shelf life.However,the molecular mechanism underlying peach softening remains largely unknown.Lateral organ boundary(LOB)domain(LBD)proteins are pivotal regulators of plant growth and development.To date,certain LOB/LBD transcription factors are seemingly implicated in fruit softening.In this study,we identified 42 LOB/LBD genes in the peach genome.Expression analysis showed a significant upregulation of PpLOB1 transcripts toward peach fruit ripening.PpLOB1 was classified into Class II subgroup,and showed high sequence similarity to several softening-related LOB/LBD transcription factors.Transient transformation assays showed that PpLOB1 positively modulates peach softening.Further experiments demonstrated that PpLOB1 directly targets and activates the promoters of pectate lyase 1(PpPL1)and PpPL15,thereby contributing to the regulation of fruit softening.Additionally,PpNAP4 up-regulates PpLOB1 expression by binding to its promoter.Meanwhile,our findings revealed that PpNAP4 and PpNAP6 cooperatively modulate the expression of Pp LOB1.Taken together,our findings revealed a novel regulatory module involving PpNAP4 and PpLOB1 that modulates peach fruit softening.展开更多
概述了LOBS-HC(labeled optical burst switch with home circuits)技术及其网络的结构,及其能提供带宽保证并提高波长带宽利用率的特性。基于Visual C++设计并实现了LOBS-HC网络仿真平台,包括网络业务流的产生和汇聚、突发包的组装、HC...概述了LOBS-HC(labeled optical burst switch with home circuits)技术及其网络的结构,及其能提供带宽保证并提高波长带宽利用率的特性。基于Visual C++设计并实现了LOBS-HC网络仿真平台,包括网络业务流的产生和汇聚、突发包的组装、HC(home circuit)逻辑链路的建立和撤销过程的模拟。通过吞吐率、网络延时、波长利用率等性能参数对LOBS-HC网络进行评测。仿真结果表明,LOBS-HC能在提供与OCS相同带宽保证的同时,获得更好的网络带宽利用率。展开更多
ASYMMETRIC LEAVES2/LATERAL ORGAN BOUNDARIES（AS2/LOB） genes are a family of plant specific transcription factors, which play an important role in the regulation of plant lateral organ development and metabolism. Howe...ASYMMETRIC LEAVES2/LATERAL ORGAN BOUNDARIES（AS2/LOB） genes are a family of plant specific transcription factors, which play an important role in the regulation of plant lateral organ development and metabolism. However, a genome-wide analysis of the AS2/LOB gene family is still not available for barley. In the present study, 24 AS2-like（ASL）/LOB domain（LBD） genes were identified based on the barley（Hordeum vulgare L.） genome sequence. A phylogenetic tree of ASL/LBD proteins from barley, Arabidopsis, maize, and rice was constructed. The ASL/LBD genes were classified into two classes, class I and class II, which were divided into five and two subgroups, respectively. Genes homologous in barley and Arabidopsis were analyzed. In addition, the structure and chromosomal locations of the genes were analyzed. Expression profiles indicated that barley HvA SL/LBD genes exhibit a variety of expression patterns, suggesting that they are involved in various aspects of physiological and developmental processes. This genome-wide analysis of the barley AS2/LOB gene family contributes to our understanding of the functions of the AS2/LOB gene family.展开更多
基金supported by grants from the China Postdoctoral Science Foundation(2023M742867)the High-level Innovation and Entrepreneurship Talents Program of QinChuangYuan,Shaanxi Province,China(QCYRCXM-2022-234)the National Key R&D Program of China(2019YFD1000203)。
文摘Softening of fleshy fruits during ripening and postharvest is a programmed physiological process that substantially impacts fruit quality and shelf life.However,the molecular mechanism underlying peach softening remains largely unknown.Lateral organ boundary(LOB)domain(LBD)proteins are pivotal regulators of plant growth and development.To date,certain LOB/LBD transcription factors are seemingly implicated in fruit softening.In this study,we identified 42 LOB/LBD genes in the peach genome.Expression analysis showed a significant upregulation of PpLOB1 transcripts toward peach fruit ripening.PpLOB1 was classified into Class II subgroup,and showed high sequence similarity to several softening-related LOB/LBD transcription factors.Transient transformation assays showed that PpLOB1 positively modulates peach softening.Further experiments demonstrated that PpLOB1 directly targets and activates the promoters of pectate lyase 1(PpPL1)and PpPL15,thereby contributing to the regulation of fruit softening.Additionally,PpNAP4 up-regulates PpLOB1 expression by binding to its promoter.Meanwhile,our findings revealed that PpNAP4 and PpNAP6 cooperatively modulate the expression of Pp LOB1.Taken together,our findings revealed a novel regulatory module involving PpNAP4 and PpLOB1 that modulates peach fruit softening.
文摘光突发交换技术和 MPL S的迅速发展产生了一种更加灵活、紧凑的 IP/ DWDM集成方案 L OBS。它综合了光路交换和光分组交换的优点及最先进的 MPL S的强大控制能力 ,是下一代因特网的理想光交换方案 ,并且为光分组交换的实现奠定了基础。文中在全面介绍 L OBS交换网络结构和控制协议的基础上 ,分析了 L OBS流量工程和 Qo S机制 ,最后初步讨论了 L
文摘概述了LOBS-HC(labeled optical burst switch with home circuits)技术及其网络的结构,及其能提供带宽保证并提高波长带宽利用率的特性。基于Visual C++设计并实现了LOBS-HC网络仿真平台,包括网络业务流的产生和汇聚、突发包的组装、HC(home circuit)逻辑链路的建立和撤销过程的模拟。通过吞吐率、网络延时、波长利用率等性能参数对LOBS-HC网络进行评测。仿真结果表明,LOBS-HC能在提供与OCS相同带宽保证的同时,获得更好的网络带宽利用率。
基金supported by the National Natural Science Foundation of China(Nos.31401370 and 31571648)the National Barley and Highland Barley Industrial Technology Specially Constructive Foundation of China(No.CARS-05)+2 种基金the Jiangsu Agriculture Science and Technology Innovation Fund(No.CX(12)5084)Jiangsu Training Programs of Innovation and Entrepreneurship for Undergraduates(No.201611117090X)the Priority Academic Program Development of Jiangsu Higher Education Institutions,China
文摘ASYMMETRIC LEAVES2/LATERAL ORGAN BOUNDARIES（AS2/LOB） genes are a family of plant specific transcription factors, which play an important role in the regulation of plant lateral organ development and metabolism. However, a genome-wide analysis of the AS2/LOB gene family is still not available for barley. In the present study, 24 AS2-like（ASL）/LOB domain（LBD） genes were identified based on the barley（Hordeum vulgare L.） genome sequence. A phylogenetic tree of ASL/LBD proteins from barley, Arabidopsis, maize, and rice was constructed. The ASL/LBD genes were classified into two classes, class I and class II, which were divided into five and two subgroups, respectively. Genes homologous in barley and Arabidopsis were analyzed. In addition, the structure and chromosomal locations of the genes were analyzed. Expression profiles indicated that barley HvA SL/LBD genes exhibit a variety of expression patterns, suggesting that they are involved in various aspects of physiological and developmental processes. This genome-wide analysis of the barley AS2/LOB gene family contributes to our understanding of the functions of the AS2/LOB gene family.
