为研究脂肪酶成熟因子1(LMF1)基因在从江香猪不同组织的表达情况及在真核细胞中的亚细胞定位情况,采用RT-PCR,Real-Time PCR,PSORT II Prediction软件结合荧光共定位的方法,克隆了从江香猪LMF1基因cDNA,构建了pEGFP-C1-LMF1真核表达载体...为研究脂肪酶成熟因子1(LMF1)基因在从江香猪不同组织的表达情况及在真核细胞中的亚细胞定位情况,采用RT-PCR,Real-Time PCR,PSORT II Prediction软件结合荧光共定位的方法,克隆了从江香猪LMF1基因cDNA,构建了pEGFP-C1-LMF1真核表达载体,分析了LMF1基因在不同组织的表达差异和亚细胞定位情况.结果表明,从江香猪LMF1基因与GenBank上提交的其他猪种的同源性达到了100%;LMF1基因在脂肪中表达量最高,心中最低,且脂肪和小肠与其他7个组织相比,表达量均存在统计学意义(p<0.01);荧光共定位结果表明,LMF1基因主要集中在细胞质中表达.相关结果对后续研究LMF1蛋白与其互作蛋白在脂质代谢的作用机理奠定的基础.展开更多
为加快收敛速度,获得稳定的性能,提出一种基于核方法的LMF(kernel least mean fourth,KLMF)多用户检测器。将DS-CDMA接收机收到的信号通过高斯核函数映射到高维特征空间(核空间),进行线性检测,并以实例在同步高斯信道环境下进行仿真试...为加快收敛速度,获得稳定的性能,提出一种基于核方法的LMF(kernel least mean fourth,KLMF)多用户检测器。将DS-CDMA接收机收到的信号通过高斯核函数映射到高维特征空间(核空间),进行线性检测,并以实例在同步高斯信道环境下进行仿真试验比较。仿真结果表明:该方法能避免高维特征空间的复杂运算,通过选择合适的核参数,KLMF检测比LMF检测具有更好的收敛性能。展开更多
Linear Modulated Frequency (LMF) Costas Stepped Frequency Radar (SFR) signal is introduced. Its ambiguity function is derived and analyzed in detail and its feasibility is validated in theory. The scheme of the propos...Linear Modulated Frequency (LMF) Costas Stepped Frequency Radar (SFR) signal is introduced. Its ambiguity function is derived and analyzed in detail and its feasibility is validated in theory. The scheme of the proposed signal processing is also presented. The results of theoretic analysis and simulation show that, by using the proposed signal and increasing the bandwidth of the total stepped frequency, the ambiguity sidelobe is well suppressed and the range-velocity coupling in the stepped frequency radar is also greatly weakened.展开更多
文摘为研究脂肪酶成熟因子1(LMF1)基因在从江香猪不同组织的表达情况及在真核细胞中的亚细胞定位情况,采用RT-PCR,Real-Time PCR,PSORT II Prediction软件结合荧光共定位的方法,克隆了从江香猪LMF1基因cDNA,构建了pEGFP-C1-LMF1真核表达载体,分析了LMF1基因在不同组织的表达差异和亚细胞定位情况.结果表明,从江香猪LMF1基因与GenBank上提交的其他猪种的同源性达到了100%;LMF1基因在脂肪中表达量最高,心中最低,且脂肪和小肠与其他7个组织相比,表达量均存在统计学意义(p<0.01);荧光共定位结果表明,LMF1基因主要集中在细胞质中表达.相关结果对后续研究LMF1蛋白与其互作蛋白在脂质代谢的作用机理奠定的基础.
文摘为加快收敛速度,获得稳定的性能,提出一种基于核方法的LMF(kernel least mean fourth,KLMF)多用户检测器。将DS-CDMA接收机收到的信号通过高斯核函数映射到高维特征空间(核空间),进行线性检测,并以实例在同步高斯信道环境下进行仿真试验比较。仿真结果表明:该方法能避免高维特征空间的复杂运算,通过选择合适的核参数,KLMF检测比LMF检测具有更好的收敛性能。
文摘Linear Modulated Frequency (LMF) Costas Stepped Frequency Radar (SFR) signal is introduced. Its ambiguity function is derived and analyzed in detail and its feasibility is validated in theory. The scheme of the proposed signal processing is also presented. The results of theoretic analysis and simulation show that, by using the proposed signal and increasing the bandwidth of the total stepped frequency, the ambiguity sidelobe is well suppressed and the range-velocity coupling in the stepped frequency radar is also greatly weakened.
