In the present study, scapaundulin C(1), a new labdane diterpenoid, and four related known compounds scapaundulin A(2), 5?, 8?, 9?-trihydroxy-13E-labden-12-one(3), 5?, 8?-dihydroxy-13E-labden-12-one(4), and(13S)-15-hy...In the present study, scapaundulin C(1), a new labdane diterpenoid, and four related known compounds scapaundulin A(2), 5?, 8?, 9?-trihydroxy-13E-labden-12-one(3), 5?, 8?-dihydroxy-13E-labden-12-one(4), and(13S)-15-hydroxylabd-8(17)-en-19-oic acid(5), were isolated from the Chinese liverwort Scapania undulate(L.) Dum., using column chromatography. The structures of these compounds were determined on the basis of 1D- and 2D-NMR analyses. The acetylcholinesterase(Ach E) inhibitory activity was evaluated using a bioautographic TLC assay and the cytotoxic activity was evaluated by the MTT method. All the compounds were reported for the first time to exhibit moderate Ach E inhibitory activity with minimal inhibitory quantities ranging from 250 to 500 ng. All the compounds were tested for their cytotoxicity against five human tumor cell lines, A549, K562, A2780, Hela, and HT29, and compounds 3 and 4 exhibited moderate inhibitory effects on the growth of A2780 cells.展开更多
Sixteen liverwort specimens collected from the Lower Cretaceous Yixian Formation of Huangbanjigou Village, Liaoning Province, China are studied in this work. The plants are thalloid and preserved in brown arenaceous m...Sixteen liverwort specimens collected from the Lower Cretaceous Yixian Formation of Huangbanjigou Village, Liaoning Province, China are studied in this work. The plants are thalloid and preserved in brown arenaceous mudstone as impressions. Based on examinations, the liverworts are assigned to two new genera and five new species： Riccardiothallus palmata sp. nov., Pallaviciniites stricta sp. nov., Pellites latithallus gen. et sp. nov., Conocephalumites hexagonites gen. et sp. nov. and Metzgerites multifidus sp. nov., belonging to five families and five genera. The fossil research indicates that the divergence of families, Aneuraceae and Metzgeriaceae, Pallaviciniaceae and Hymenophytaceae,Pelliaceae and Fossombroniaceae, was in the Lower Cretaceous（125 Ma）. The research provides significant additions to the fossil liverwort records in Western Liaoning and offers fossil evidence for studying the classification and evolution of extant liverworts.展开更多
Paper includes information on population status of Conocephalum conicum collected from diverse habitats in Bhader-wah (J & K state). Taxon exhibits tremendous diversity in morphoanatomical details of various gamet...Paper includes information on population status of Conocephalum conicum collected from diverse habitats in Bhader-wah (J & K state). Taxon exhibits tremendous diversity in morphoanatomical details of various gametophytic charac-ters. Isozyme analysis was performed to find out whether variability in the taxon is only environmental or has genetic basis as well.展开更多
Two distinctive rearranged 19-nor-7,8-seco-labdane diterpenoids(1 and 2)with a novel tetracyclo[5.2.1.0^(2,5.)0^(4,10)]decane skeleton,a derivative of the open tetrahydrofuran ring(7),three dimeric compounds(8-10),and...Two distinctive rearranged 19-nor-7,8-seco-labdane diterpenoids(1 and 2)with a novel tetracyclo[5.2.1.0^(2,5.)0^(4,10)]decane skeleton,a derivative of the open tetrahydrofuran ring(7),three dimeric compounds(8-10),and four revised homologs(3-6)were obtained from Chinese liverwort Pallavicinia ambigua.Their structures were identified via combined analysis of their spectroscopic data,single-crystal X-ray diffraction patterns,and ECD calculations.The light-driven conversion of compound 5 to compounds 1-4 demonstrated that photochemically induced postmodification involved in biosynthesis is an important way to diversify natural structures.A preliminary cytotoxicity assay revealed that compound 5 showed significant inhibition in the human prostate cancer(PC-3)cell line via an apoptotic pathway.展开更多
New bioactive 13-epi-neoverrucosane diterpenoid,5β-acetoxy-13-epi-neoverrucosanic acid(1)along with three known secondary metabolites,13-epi-neoverrucosan-5β-ol(2),chelodane(3)and(E)-β-farnesene(4)were isolated fro...New bioactive 13-epi-neoverrucosane diterpenoid,5β-acetoxy-13-epi-neoverrucosanic acid(1)along with three known secondary metabolites,13-epi-neoverrucosan-5β-ol(2),chelodane(3)and(E)-β-farnesene(4)were isolated from the MeOH extract of east Malaysia’s liverwort Pleurozia subinfata.The chemical structure of new compound was elucidated by the analyses of its spectroscopic data(FTIR,NMR and HR-ESI-MS).These epi-neoverrucosane-type compounds seem to be notable chemosystematic markers for P.subinfata in Borneo.Compound 3 was widespread in marine sponges however this is the frst record for 3 to be found in liverwort.These metabolites were tested for their antifungal potentials against selected fungi from the marine environment.Compound 1 exhibited efective antifungal activity against Lagenidium thermophilum.展开更多
The spore morphology of 9 species of 3 genera of Chinese liverworts was studied by scanning electron microscopic observation. They are Riccia huebeneriana Lindenb., R. hantamensis Perold., R. cavernosa Hoffm., R. warn...The spore morphology of 9 species of 3 genera of Chinese liverworts was studied by scanning electron microscopic observation. They are Riccia huebeneriana Lindenb., R. hantamensis Perold., R. cavernosa Hoffm., R. warnstorfii Limpr. ex Warnst., R. frostii Aust., R. chinensis Herz., Asterella yoshinagana (Horik.) Horik., A. sanguinia L. et L., and Preissia quadrata (Scop.) Nees. There were evident morphological differences in spores among the above three genera. Difference of species morphology in the same genus was also observed. Therefore in the above genera and species the spore-wall ornamentation can be regarded as a criterion for taxonomic specificity. Meanwhile, slight difference in morphological characters of the same species collected from different sites was observed and reckoned to be an ecological adaptation.展开更多
Epiphyllous liverworts are found in Yangjia Brook in Houhe National Nature Reserve( 110° 33′10″ E , 30°5′32″ N) , Hubei province recently,which distribute in the evergreen broadleaved forests at 1 000～1...Epiphyllous liverworts are found in Yangjia Brook in Houhe National Nature Reserve( 110° 33′10″ E , 30°5′32″ N) , Hubei province recently,which distribute in the evergreen broadleaved forests at 1 000～1 050 m above sea level. It is the first report of the most northern boundary line of the distribution of the epiphyllous liverworts in China.展开更多
目的对硬指叶苔L ep id oz ia v itrea的醇提物和水提物的化学成分进行研究,从中寻找生物活性成分。方法采用柱色谱方法进行化合物分离,通过波谱方法确定化合物结构。结果从其乙醇提取物中分离得到植物醇(Ⅰ)、7-羟基去氢白菖烯(Ⅱ)、...目的对硬指叶苔L ep id oz ia v itrea的醇提物和水提物的化学成分进行研究,从中寻找生物活性成分。方法采用柱色谱方法进行化合物分离,通过波谱方法确定化合物结构。结果从其乙醇提取物中分离得到植物醇(Ⅰ)、7-羟基去氢白菖烯(Ⅱ)、瑞香内酯(Ⅲ)、胡萝卜苷(Ⅳ);从其水提取物中分离得到两个多元醇类化合物,分别是D-g lycero-d-ga lacto-heptito l(Ⅴ)、D-erythro-L-ga lacto-octito l(Ⅵ)。结论6个化合物均为首次从该植物中分离得到。展开更多
基金financially supported by National Natural Science Foundation of China(No.30925038)
文摘In the present study, scapaundulin C(1), a new labdane diterpenoid, and four related known compounds scapaundulin A(2), 5?, 8?, 9?-trihydroxy-13E-labden-12-one(3), 5?, 8?-dihydroxy-13E-labden-12-one(4), and(13S)-15-hydroxylabd-8(17)-en-19-oic acid(5), were isolated from the Chinese liverwort Scapania undulate(L.) Dum., using column chromatography. The structures of these compounds were determined on the basis of 1D- and 2D-NMR analyses. The acetylcholinesterase(Ach E) inhibitory activity was evaluated using a bioautographic TLC assay and the cytotoxic activity was evaluated by the MTT method. All the compounds were reported for the first time to exhibit moderate Ach E inhibitory activity with minimal inhibitory quantities ranging from 250 to 500 ng. All the compounds were tested for their cytotoxicity against five human tumor cell lines, A549, K562, A2780, Hela, and HT29, and compounds 3 and 4 exhibited moderate inhibitory effects on the growth of A2780 cells.
基金funded by Ministry of Science and Technology(2015FY310100)Natural Science Foundation of China(41402023,41472030)China Geological Survey(DD20160120-04,DD20160345)
文摘Sixteen liverwort specimens collected from the Lower Cretaceous Yixian Formation of Huangbanjigou Village, Liaoning Province, China are studied in this work. The plants are thalloid and preserved in brown arenaceous mudstone as impressions. Based on examinations, the liverworts are assigned to two new genera and five new species： Riccardiothallus palmata sp. nov., Pallaviciniites stricta sp. nov., Pellites latithallus gen. et sp. nov., Conocephalumites hexagonites gen. et sp. nov. and Metzgerites multifidus sp. nov., belonging to five families and five genera. The fossil research indicates that the divergence of families, Aneuraceae and Metzgeriaceae, Pallaviciniaceae and Hymenophytaceae,Pelliaceae and Fossombroniaceae, was in the Lower Cretaceous（125 Ma）. The research provides significant additions to the fossil liverwort records in Western Liaoning and offers fossil evidence for studying the classification and evolution of extant liverworts.
文摘Paper includes information on population status of Conocephalum conicum collected from diverse habitats in Bhader-wah (J & K state). Taxon exhibits tremendous diversity in morphoanatomical details of various gametophytic charac-ters. Isozyme analysis was performed to find out whether variability in the taxon is only environmental or has genetic basis as well.
基金national financial support from the National Key R&D Program of China(No.2019YFA0905700)the National Natural Science Foundation of China(Nos.82173703 and 81874293)the Major Basic Research Program of Shandong Provincial Natural Science Foundation(No.ZR2019ZD26)。
文摘Two distinctive rearranged 19-nor-7,8-seco-labdane diterpenoids(1 and 2)with a novel tetracyclo[5.2.1.0^(2,5.)0^(4,10)]decane skeleton,a derivative of the open tetrahydrofuran ring(7),three dimeric compounds(8-10),and four revised homologs(3-6)were obtained from Chinese liverwort Pallavicinia ambigua.Their structures were identified via combined analysis of their spectroscopic data,single-crystal X-ray diffraction patterns,and ECD calculations.The light-driven conversion of compound 5 to compounds 1-4 demonstrated that photochemically induced postmodification involved in biosynthesis is an important way to diversify natural structures.A preliminary cytotoxicity assay revealed that compound 5 showed significant inhibition in the human prostate cancer(PC-3)cell line via an apoptotic pathway.
基金supported by SaBC Grant[No.GL0070]and UMS Grant[SBK0258-SG-2016].
文摘New bioactive 13-epi-neoverrucosane diterpenoid,5β-acetoxy-13-epi-neoverrucosanic acid(1)along with three known secondary metabolites,13-epi-neoverrucosan-5β-ol(2),chelodane(3)and(E)-β-farnesene(4)were isolated from the MeOH extract of east Malaysia’s liverwort Pleurozia subinfata.The chemical structure of new compound was elucidated by the analyses of its spectroscopic data(FTIR,NMR and HR-ESI-MS).These epi-neoverrucosane-type compounds seem to be notable chemosystematic markers for P.subinfata in Borneo.Compound 3 was widespread in marine sponges however this is the frst record for 3 to be found in liverwort.These metabolites were tested for their antifungal potentials against selected fungi from the marine environment.Compound 1 exhibited efective antifungal activity against Lagenidium thermophilum.
文摘The spore morphology of 9 species of 3 genera of Chinese liverworts was studied by scanning electron microscopic observation. They are Riccia huebeneriana Lindenb., R. hantamensis Perold., R. cavernosa Hoffm., R. warnstorfii Limpr. ex Warnst., R. frostii Aust., R. chinensis Herz., Asterella yoshinagana (Horik.) Horik., A. sanguinia L. et L., and Preissia quadrata (Scop.) Nees. There were evident morphological differences in spores among the above three genera. Difference of species morphology in the same genus was also observed. Therefore in the above genera and species the spore-wall ornamentation can be regarded as a criterion for taxonomic specificity. Meanwhile, slight difference in morphological characters of the same species collected from different sites was observed and reckoned to be an ecological adaptation.
文摘Epiphyllous liverworts are found in Yangjia Brook in Houhe National Nature Reserve( 110° 33′10″ E , 30°5′32″ N) , Hubei province recently,which distribute in the evergreen broadleaved forests at 1 000～1 050 m above sea level. It is the first report of the most northern boundary line of the distribution of the epiphyllous liverworts in China.
文摘目的对硬指叶苔L ep id oz ia v itrea的醇提物和水提物的化学成分进行研究,从中寻找生物活性成分。方法采用柱色谱方法进行化合物分离,通过波谱方法确定化合物结构。结果从其乙醇提取物中分离得到植物醇(Ⅰ)、7-羟基去氢白菖烯(Ⅱ)、瑞香内酯(Ⅲ)、胡萝卜苷(Ⅳ);从其水提取物中分离得到两个多元醇类化合物,分别是D-g lycero-d-ga lacto-heptito l(Ⅴ)、D-erythro-L-ga lacto-octito l(Ⅵ)。结论6个化合物均为首次从该植物中分离得到。
