Listeriosis is an illness caused by the germ<span style="font-family:Verdana;"> Listeria monocytogenes<span style="font-family:Verdana;">. Generally, humans are infected with listeriosi...Listeriosis is an illness caused by the germ<span style="font-family:Verdana;"> Listeria monocytogenes<span style="font-family:Verdana;">. Generally, humans are infected with listeriosis after eating contaminated food. Listeriosis mostly affects people with weakened immune systems, pregnant women and newborns. In this paper, a model describing the dynamics o<span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">f Listeriosis is developed and analysed using ordinary differential equations. The model was analysed both quantitatively and qualitatively for its local and global stability, basic reproductive number and parameter contributions to the basic reproductive number to understand the impact of each parameter on the disease spread. The Listeriosis model has been extended to include time dependent control variables such as treatment of both humans and animals, vaccination and education of humans. Pontryagin<span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">’<span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">s Maximum Principle was introduced to obtain the best optimal control strategies required for curbing Listeriosis infections. Numerical simulation was performed and the results displayed graphically and discussed. Cost effectiveness analysis was conducted using the intervention averted ratio (IAR) concepts and it was revealed that the most effective intervention strategy is the treatment of infect<span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">ed<span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"> humans and animals.展开更多
<strong>Background:</strong> Listeriosis affects immunosuppressed individual’s especially pregnant women. Maternal infections are usually mild for the woman, but may have devastating effects on the unborn...<strong>Background:</strong> Listeriosis affects immunosuppressed individual’s especially pregnant women. Maternal infections are usually mild for the woman, but may have devastating effects on the unborn child, including miscarriage, stillbirth, preterm labour and serious neonatal illness. WHO recommends that pregnant women should be educated to avoid foods with high risk of contamination. This study seeks to evaluate knowledge and practices predisposing to listeria infections during pregnancy. <strong>Methodology:</strong> This is a cross-sectional study on pregnant women, attending the antenatal clinic of Lagos University Teaching Hospital (LUTH) using semi structured questionnaires. The knowledge score of participants was collated and analyzed using SPSS version 26. <strong>Results:</strong> The mean age of the participants was 32.07 ± 5.6 years. Forty-three (30.7%) women have had previous miscarriages with the mean no of miscarriage of 1.54. Out of 147 participants, only 20 (13.6%) admitted having heard of listeriosis while 127 (86.4%) had never heard of listeriosis and none of the participants has ever been tested for listeriosis. The Overall knowledge score was poor, and this was irrespective of age and duration of pregnancy. However, women with graduate and/or postgraduate degrees were more likely to have heard of Listeriosis (X2 = 10.88, P = 0.028). <strong>Conclusion:</strong> Our study shows a low level of knowledge about this food-borne illness, which can lead to severe illnesses in pregnant women and their unborn child. It is necessarily to educate pregnant women on the risk of listeriosis. This would be a key factor in creating and implementing accurate measures of prevention and control.展开更多
Objective:To identify priority targets for listeriosis surveillance in China by leveraging insights from the European Union(EU)surveillance system.Methods:We conducted a systematic,macro-pattern comparison between int...Objective:To identify priority targets for listeriosis surveillance in China by leveraging insights from the European Union(EU)surveillance system.Methods:We conducted a systematic,macro-pattern comparison between integrated surveillance data from China and the EU.Given the substantial heterogeneity inherent in the Chinese data,a direct quantitative comparison between the two regions was unfeasible.Therefore,the analysis focused on identifying and contrasting broad epidemiological patterns.Particular attention was paid to patterns discernible from the EU data that could inform risk assessment in China,such as the identification of high-risk groups.To quantify one such key pattern within the EU dataset,the Cochran-Armitage trend test was used to assess the long-term trend in the proportion of cases aged 65 and above.A linear regression model was then applied to estimate the annual percentage change in this proportion over time.Results:Most available data on listeriosis in China come from published literature.Consequently,the country lacks accurate disease burden statistics.Over the period 2007-2023,the EU reported a cumulative total of 33,497 listeriosis cases.Both the demographic and serotype distributions showed clear predominance:individuals aged 65 and above constituted the largest proportion across all reported outcomes,while serotypes 4b,4d and 4e together accounted for nearly 50%of cases.The EU has achieved full geographic and population coverage.In contrast,China's surveillance remains fragmented and non-representative,with significant geographic and demographic gaps.Conclusion:The experience of the EU provides critical clues for identifying high-risk populations and key serotypes.We should thoroughly analyze surveillance data from sentinel hospitals to systematically identify local high-risk groups(such as individuals aged 65 and above)and high-risk food categories.Building on this,we can progressively advance the development of a traceability system,expand surveillance coverage,and strengthen the refinement of relevant regulations and management frameworks.展开更多
This study aimed to investigate the prevalence and molecular characteristics ofListeria monocytogenes in cooked products in Zigong City, China. The overall occurrence of the L. monocytogenes in the ready-to-eat （RTE...This study aimed to investigate the prevalence and molecular characteristics ofListeria monocytogenes in cooked products in Zigong City, China. The overall occurrence of the L. monocytogenes in the ready-to-eat （RTE） shops and mutton restaurants surveyed was 16.2% （141/873）. An occurrence of 13.5% was observed in RTE pork, 6.5% in RTE vegetables, and more than 24.0% in either cooked mutton or cooked haggis. Serotype 1/2b （45.4%）, 1/2a （33.3%）, and 1/2c （14.2%） were the predominant types. By comparing the clonal complexes （CCs） based on multilocus sequence typing （MLST） of the L. monocytogenes from cooked foods in Zigong City and 33 listeriosis cases from different districts of China, CC87, CC9, CC8, and CC3 were showed to be prevalent in cooked products and CC87 and CC3 were the first two frequent types in the 33 clinic-source strains. All CC87 stains harbored the newly reported Listeria pathogenicity island 4 （LIPI-4） gene fragment ptsA, and all CC3 strains possessed the Listeria pathogenicity island 3 （LIPI-3） gene fragment llsX. These may increase the occurrence of the strains belonging to CC87 and CC3 in listeriosis cases in China and also underline the risk of infection owing to the consumption of the cooked products from Zigong. ST619 （serotype 1/2b） harbored both llsX and ptsA, indicating a potential hypervirulent sequence type in Zigong.展开更多
<strong>Background:</strong> Listeriosis is a common zoonotic disease caused by a foodborne pathogen, <em>Listeria monocytogenes</em>. Poultry meat and products have been established as vehicle...<strong>Background:</strong> Listeriosis is a common zoonotic disease caused by a foodborne pathogen, <em>Listeria monocytogenes</em>. Poultry meat and products have been established as vehicles of transmission of pathogenic <em>Listeria</em> strains to humans. This study evaluates the occurrence of <em>Listeria species</em> in faeces of poultry chicken in Lagos. <strong>Methods:</strong> One hundred and fourteen pooled fresh faecal samples from cage-reared broiler chickens were collected from 12 farms in three rural areas in Lagos State from May to August 2019. All samples were analysed for <em>Listeria</em> species detection according to ISO11290-1 standard and confirmed using PCR assay. Susceptibility testing was performed using the Kirby-Bauer disc diffusion technique. <strong>Results:</strong> Twenty-eight (24.6%) <em>Listeria </em>species were detected from 114 faecal samples. The isolated <em>Listeria</em> species were<em> L. monocytogenes</em> 8 (7.0%), <em>L. ivanovii</em> 9 (7.9%),<em> L. grayi </em>7 (6.1%) and<em> L. innocua</em> 4 (3.5%). There was no significant difference in the frequency of occurrence of <em>Listeria</em> species across the different locations (X<sup>2</sup> = 4.98, p = 0.08). The listeria species were susceptible to Augmentin (96.4%), vancomycin (85.7%) and co-trimoxazole (82.1%), but resistant to ceftazidime (100%), tetracycline (75.0%) and ciprofloxacin (71.4%). <strong>Conclusion:</strong> This study reveals high occurrence of multi-drug resistant <em>Listeria</em> species in faecal samples of poultry chickens in Lagos state which may be an important vector in the contamination of the environment and transmission of antibiotic resistant <em>Listeria</em> species to consumers.展开更多
Listeria monocytogenes(L.monocytogenes),a foodborne pathogen,can cause human listeriosis.Listeriosis is a potentially fatal gastrointestinal illness,which is closely related to the spread of food to humans.We review t...Listeria monocytogenes(L.monocytogenes),a foodborne pathogen,can cause human listeriosis.Listeriosis is a potentially fatal gastrointestinal illness,which is closely related to the spread of food to humans.We review the literature published during 2010-2019 to better understand the prevalence of L.monocytogenes in food products,incidence of human listeriosis,and their characteristics in Chinese mainland.We found the main sequence types(STs)strains from foods are similar globally,and the prevalence of L.monocytogenes from raw meat was the highest among all food products.The most common STs in food products and clinical cases were ST9(serogroup I.2)strains and ST87(serogroup Il.2)strains,respectively.The ST87 strains being the most common STs of clinical cases might be related to the existence of Listeria pathogenicity island 4 gene and Chinese eating habits for ready-to-eat foods,among which the prevalence of ST87 strain was the highest in ready-to-eat foods.Therefore,more research should be conducted to explore the reasons for the L.monocytogenes isolates differences in food and clinical sources.展开更多
文摘Listeriosis is an illness caused by the germ<span style="font-family:Verdana;"> Listeria monocytogenes<span style="font-family:Verdana;">. Generally, humans are infected with listeriosis after eating contaminated food. Listeriosis mostly affects people with weakened immune systems, pregnant women and newborns. In this paper, a model describing the dynamics o<span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">f Listeriosis is developed and analysed using ordinary differential equations. The model was analysed both quantitatively and qualitatively for its local and global stability, basic reproductive number and parameter contributions to the basic reproductive number to understand the impact of each parameter on the disease spread. The Listeriosis model has been extended to include time dependent control variables such as treatment of both humans and animals, vaccination and education of humans. Pontryagin<span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">’<span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">s Maximum Principle was introduced to obtain the best optimal control strategies required for curbing Listeriosis infections. Numerical simulation was performed and the results displayed graphically and discussed. Cost effectiveness analysis was conducted using the intervention averted ratio (IAR) concepts and it was revealed that the most effective intervention strategy is the treatment of infect<span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">ed<span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"> humans and animals.
文摘<strong>Background:</strong> Listeriosis affects immunosuppressed individual’s especially pregnant women. Maternal infections are usually mild for the woman, but may have devastating effects on the unborn child, including miscarriage, stillbirth, preterm labour and serious neonatal illness. WHO recommends that pregnant women should be educated to avoid foods with high risk of contamination. This study seeks to evaluate knowledge and practices predisposing to listeria infections during pregnancy. <strong>Methodology:</strong> This is a cross-sectional study on pregnant women, attending the antenatal clinic of Lagos University Teaching Hospital (LUTH) using semi structured questionnaires. The knowledge score of participants was collated and analyzed using SPSS version 26. <strong>Results:</strong> The mean age of the participants was 32.07 ± 5.6 years. Forty-three (30.7%) women have had previous miscarriages with the mean no of miscarriage of 1.54. Out of 147 participants, only 20 (13.6%) admitted having heard of listeriosis while 127 (86.4%) had never heard of listeriosis and none of the participants has ever been tested for listeriosis. The Overall knowledge score was poor, and this was irrespective of age and duration of pregnancy. However, women with graduate and/or postgraduate degrees were more likely to have heard of Listeriosis (X2 = 10.88, P = 0.028). <strong>Conclusion:</strong> Our study shows a low level of knowledge about this food-borne illness, which can lead to severe illnesses in pregnant women and their unborn child. It is necessarily to educate pregnant women on the risk of listeriosis. This would be a key factor in creating and implementing accurate measures of prevention and control.
基金supported by the Fujian Provincial Health Commission Science and Technology Project(No.2023GGA041)the Fujian Research and Training Grants for Young and Middle-aged Leaders in Healthcare(No.[2023]2839).
文摘Objective:To identify priority targets for listeriosis surveillance in China by leveraging insights from the European Union(EU)surveillance system.Methods:We conducted a systematic,macro-pattern comparison between integrated surveillance data from China and the EU.Given the substantial heterogeneity inherent in the Chinese data,a direct quantitative comparison between the two regions was unfeasible.Therefore,the analysis focused on identifying and contrasting broad epidemiological patterns.Particular attention was paid to patterns discernible from the EU data that could inform risk assessment in China,such as the identification of high-risk groups.To quantify one such key pattern within the EU dataset,the Cochran-Armitage trend test was used to assess the long-term trend in the proportion of cases aged 65 and above.A linear regression model was then applied to estimate the annual percentage change in this proportion over time.Results:Most available data on listeriosis in China come from published literature.Consequently,the country lacks accurate disease burden statistics.Over the period 2007-2023,the EU reported a cumulative total of 33,497 listeriosis cases.Both the demographic and serotype distributions showed clear predominance:individuals aged 65 and above constituted the largest proportion across all reported outcomes,while serotypes 4b,4d and 4e together accounted for nearly 50%of cases.The EU has achieved full geographic and population coverage.In contrast,China's surveillance remains fragmented and non-representative,with significant geographic and demographic gaps.Conclusion:The experience of the EU provides critical clues for identifying high-risk populations and key serotypes.We should thoroughly analyze surveillance data from sentinel hospitals to systematically identify local high-risk groups(such as individuals aged 65 and above)and high-risk food categories.Building on this,we can progressively advance the development of a traceability system,expand surveillance coverage,and strengthen the refinement of relevant regulations and management frameworks.
文摘This study aimed to investigate the prevalence and molecular characteristics ofListeria monocytogenes in cooked products in Zigong City, China. The overall occurrence of the L. monocytogenes in the ready-to-eat （RTE） shops and mutton restaurants surveyed was 16.2% （141/873）. An occurrence of 13.5% was observed in RTE pork, 6.5% in RTE vegetables, and more than 24.0% in either cooked mutton or cooked haggis. Serotype 1/2b （45.4%）, 1/2a （33.3%）, and 1/2c （14.2%） were the predominant types. By comparing the clonal complexes （CCs） based on multilocus sequence typing （MLST） of the L. monocytogenes from cooked foods in Zigong City and 33 listeriosis cases from different districts of China, CC87, CC9, CC8, and CC3 were showed to be prevalent in cooked products and CC87 and CC3 were the first two frequent types in the 33 clinic-source strains. All CC87 stains harbored the newly reported Listeria pathogenicity island 4 （LIPI-4） gene fragment ptsA, and all CC3 strains possessed the Listeria pathogenicity island 3 （LIPI-3） gene fragment llsX. These may increase the occurrence of the strains belonging to CC87 and CC3 in listeriosis cases in China and also underline the risk of infection owing to the consumption of the cooked products from Zigong. ST619 （serotype 1/2b） harbored both llsX and ptsA, indicating a potential hypervirulent sequence type in Zigong.
文摘<strong>Background:</strong> Listeriosis is a common zoonotic disease caused by a foodborne pathogen, <em>Listeria monocytogenes</em>. Poultry meat and products have been established as vehicles of transmission of pathogenic <em>Listeria</em> strains to humans. This study evaluates the occurrence of <em>Listeria species</em> in faeces of poultry chicken in Lagos. <strong>Methods:</strong> One hundred and fourteen pooled fresh faecal samples from cage-reared broiler chickens were collected from 12 farms in three rural areas in Lagos State from May to August 2019. All samples were analysed for <em>Listeria</em> species detection according to ISO11290-1 standard and confirmed using PCR assay. Susceptibility testing was performed using the Kirby-Bauer disc diffusion technique. <strong>Results:</strong> Twenty-eight (24.6%) <em>Listeria </em>species were detected from 114 faecal samples. The isolated <em>Listeria</em> species were<em> L. monocytogenes</em> 8 (7.0%), <em>L. ivanovii</em> 9 (7.9%),<em> L. grayi </em>7 (6.1%) and<em> L. innocua</em> 4 (3.5%). There was no significant difference in the frequency of occurrence of <em>Listeria</em> species across the different locations (X<sup>2</sup> = 4.98, p = 0.08). The listeria species were susceptible to Augmentin (96.4%), vancomycin (85.7%) and co-trimoxazole (82.1%), but resistant to ceftazidime (100%), tetracycline (75.0%) and ciprofloxacin (71.4%). <strong>Conclusion:</strong> This study reveals high occurrence of multi-drug resistant <em>Listeria</em> species in faecal samples of poultry chickens in Lagos state which may be an important vector in the contamination of the environment and transmission of antibiotic resistant <em>Listeria</em> species to consumers.
基金supported by the National Key Research and Development Program of China (No.2019YFE0103800).
文摘Listeria monocytogenes(L.monocytogenes),a foodborne pathogen,can cause human listeriosis.Listeriosis is a potentially fatal gastrointestinal illness,which is closely related to the spread of food to humans.We review the literature published during 2010-2019 to better understand the prevalence of L.monocytogenes in food products,incidence of human listeriosis,and their characteristics in Chinese mainland.We found the main sequence types(STs)strains from foods are similar globally,and the prevalence of L.monocytogenes from raw meat was the highest among all food products.The most common STs in food products and clinical cases were ST9(serogroup I.2)strains and ST87(serogroup Il.2)strains,respectively.The ST87 strains being the most common STs of clinical cases might be related to the existence of Listeria pathogenicity island 4 gene and Chinese eating habits for ready-to-eat foods,among which the prevalence of ST87 strain was the highest in ready-to-eat foods.Therefore,more research should be conducted to explore the reasons for the L.monocytogenes isolates differences in food and clinical sources.
