Objective:To screen the blood group system genes of Duffy,Lutheran,Kidd,Diego,Dombrock blood group systems of Li ethnic group in Hainan Province and provide laboratory data for the rare blood group database in this ar...Objective:To screen the blood group system genes of Duffy,Lutheran,Kidd,Diego,Dombrock blood group systems of Li ethnic group in Hainan Province and provide laboratory data for the rare blood group database in this area.Methods:The alleles of Duffy,Lutheran,Kidd,Diego,Dombrock blood group systems of 300 voluntary participants of Li ethnic group in Hainan were detected by sequence-specific primer polymerase chain reaction,and the polymorphism was analyzed.Results:The allele frequencies of Duffy,Lutheran,Kidd,Diego,Dombrock blood group systems of Li ethnic groups in Hainan Province are 0.9583 for Fy^(a),0.0417 for Fy^(b),0.8350 for Au^(a),0.1650 for Au^(b),0.4500 for Jk^(a),0.5500 for Jk^(b),0.0667 for Di^(a),0.9333 for Di^(b),0.1017 for Doa and 0.8983 for Dob,respectively.The antigen incompatibility rates of Fy^(a)/Fy^(b),Au^(a)/Au^(b),Jk^(a)/Jk^(b),Di^(a)/Di^(b),Doa/Dob of Duffy,Lutheran,Kidd,Diego,Dombrock blood group systems were 7.67%,23.76%,37.25%,11.67%and 16.60%,respectively.Conclusion:The gene frequencies of Duffy,Lutheran,Kidd,Diego,Dombrock blood group systems of Li ethnic group in Hainan Province are polymorphic,and the antigen incompatibility rates of alleles are higher,which is quite different from that of other nationalities in China and with unique ethnic distribution characteristics.It is of great significance to establish the rare blood group database in this region.展开更多
Cadastral maps are an important component of land administration in most countries. In virtually all developed countries, the needs of computerized land and geographic information systems (LIS/GIS) has given urgent im...Cadastral maps are an important component of land administration in most countries. In virtually all developed countries, the needs of computerized land and geographic information systems (LIS/GIS) has given urgent impetus to computerizing cadastral maps and creating digital cadastral data bases (DCDB). This process is creating many institutional, legal, technical and administrative problems. This desire to establish DCDBs is being given increased impetus due to a new range of enabling technologies such as satellite position fixing (GPS), improved spatial data collection techniques such as digital theodolites and “soft copy” photogrammetry, as well as a vast range of new information and communications technological tools, thus contributing to the advancement and keeping up with the great countries. This paper presents the problem of cadastral maps. The hitherto existing cadastre, consisting of paper maps and land registers, is now becoming insufficient. Its shortcomings force developments leading to its improvement. One of the ways is the creation of a Land Information System. A digital cadastral map is the main component of this system. The structure and information content of the map is presented, its differences from analogue maps are shown, and the process of map creation is described. A digital cadastral map can be the basis for additional thematic layers, successively converting it into a complex system for management of administrative units.展开更多
Agrobacterium tumefaciens-mediated transformation has been widely adopted for plant genetic engineering and the study of gene function(Krenek et al.,2015).This method is prevalent in the genetic transformation of herb...Agrobacterium tumefaciens-mediated transformation has been widely adopted for plant genetic engineering and the study of gene function(Krenek et al.,2015).This method is prevalent in the genetic transformation of herbaceous plants,with notable applications in species such as Arabidopsis(Yin et al.,2024),soybean(Zhang et al.,2024),rice(Zhang et al.,2020),and Chinese cabbage(Li et al.,2021).However,its application in fruit trees is limited.This is primarily due to their long growth cycles and lack of rapid,efficient,and stable transgenic systems,which severely hinders foundational research involving plant genetic transformation(Mei et al.,2024).Furthermore,for subtropical fruit trees,the presence of recalcitrant seeds adds an extra layer of difficulty to genetic transformation(Umarani et al.,2015),as most methods rely on seed germination as a basis for transformation.展开更多
当前，现代检验医学已步入了一个以自动化、信息化、网络化为主要特征的新时期。检验水平和检测手段日新月异，临床检验工作日趋自动化、规范化、信息化，因而建立一套功能强大的实验室信息系统（laboratory information system，LIS）...当前，现代检验医学已步入了一个以自动化、信息化、网络化为主要特征的新时期。检验水平和检测手段日新月异，临床检验工作日趋自动化、规范化、信息化，因而建立一套功能强大的实验室信息系统（laboratory information system，LIS）是现代临床实验室必不可少的。如今的LIS已不仅仅局限于标本管理、数据采集、报告打印等简单的功能，信息技术的发展、医疗体制改革的要求以及各种严格的行业规范，将LIS推向了前所未有的高度。展开更多
基金Hainan Provincial Natural Science Foundation of China(No.820QN410)Hainan Province Clinical Medical Center(No.QWYH202175)。
文摘Objective:To screen the blood group system genes of Duffy,Lutheran,Kidd,Diego,Dombrock blood group systems of Li ethnic group in Hainan Province and provide laboratory data for the rare blood group database in this area.Methods:The alleles of Duffy,Lutheran,Kidd,Diego,Dombrock blood group systems of 300 voluntary participants of Li ethnic group in Hainan were detected by sequence-specific primer polymerase chain reaction,and the polymorphism was analyzed.Results:The allele frequencies of Duffy,Lutheran,Kidd,Diego,Dombrock blood group systems of Li ethnic groups in Hainan Province are 0.9583 for Fy^(a),0.0417 for Fy^(b),0.8350 for Au^(a),0.1650 for Au^(b),0.4500 for Jk^(a),0.5500 for Jk^(b),0.0667 for Di^(a),0.9333 for Di^(b),0.1017 for Doa and 0.8983 for Dob,respectively.The antigen incompatibility rates of Fy^(a)/Fy^(b),Au^(a)/Au^(b),Jk^(a)/Jk^(b),Di^(a)/Di^(b),Doa/Dob of Duffy,Lutheran,Kidd,Diego,Dombrock blood group systems were 7.67%,23.76%,37.25%,11.67%and 16.60%,respectively.Conclusion:The gene frequencies of Duffy,Lutheran,Kidd,Diego,Dombrock blood group systems of Li ethnic group in Hainan Province are polymorphic,and the antigen incompatibility rates of alleles are higher,which is quite different from that of other nationalities in China and with unique ethnic distribution characteristics.It is of great significance to establish the rare blood group database in this region.
文摘Cadastral maps are an important component of land administration in most countries. In virtually all developed countries, the needs of computerized land and geographic information systems (LIS/GIS) has given urgent impetus to computerizing cadastral maps and creating digital cadastral data bases (DCDB). This process is creating many institutional, legal, technical and administrative problems. This desire to establish DCDBs is being given increased impetus due to a new range of enabling technologies such as satellite position fixing (GPS), improved spatial data collection techniques such as digital theodolites and “soft copy” photogrammetry, as well as a vast range of new information and communications technological tools, thus contributing to the advancement and keeping up with the great countries. This paper presents the problem of cadastral maps. The hitherto existing cadastre, consisting of paper maps and land registers, is now becoming insufficient. Its shortcomings force developments leading to its improvement. One of the ways is the creation of a Land Information System. A digital cadastral map is the main component of this system. The structure and information content of the map is presented, its differences from analogue maps are shown, and the process of map creation is described. A digital cadastral map can be the basis for additional thematic layers, successively converting it into a complex system for management of administrative units.
基金funded by the Key-Area Research and Development Program of Guangdong Province(Grant No.2022B0202070002)the Guangxi Science and Technology Major Program(Grant No.GuikeAA23023007-2)+1 种基金the Guangdong Province Modern Agricultural Industry Technology System Innovation Team Construction Project(2024CXTD19)Guangdong Basic and Applied Basic Research Foundation(Grant No.2023A1515010303)。
文摘Agrobacterium tumefaciens-mediated transformation has been widely adopted for plant genetic engineering and the study of gene function(Krenek et al.,2015).This method is prevalent in the genetic transformation of herbaceous plants,with notable applications in species such as Arabidopsis(Yin et al.,2024),soybean(Zhang et al.,2024),rice(Zhang et al.,2020),and Chinese cabbage(Li et al.,2021).However,its application in fruit trees is limited.This is primarily due to their long growth cycles and lack of rapid,efficient,and stable transgenic systems,which severely hinders foundational research involving plant genetic transformation(Mei et al.,2024).Furthermore,for subtropical fruit trees,the presence of recalcitrant seeds adds an extra layer of difficulty to genetic transformation(Umarani et al.,2015),as most methods rely on seed germination as a basis for transformation.
文摘当前，现代检验医学已步入了一个以自动化、信息化、网络化为主要特征的新时期。检验水平和检测手段日新月异，临床检验工作日趋自动化、规范化、信息化，因而建立一套功能强大的实验室信息系统（laboratory information system，LIS）是现代临床实验室必不可少的。如今的LIS已不仅仅局限于标本管理、数据采集、报告打印等简单的功能，信息技术的发展、医疗体制改革的要求以及各种严格的行业规范，将LIS推向了前所未有的高度。
