Agrobacterium tumefaciens-mediated transformation has been widely adopted for plant genetic engineering and the study of gene function(Krenek et al.,2015).This method is prevalent in the genetic transformation of herb...Agrobacterium tumefaciens-mediated transformation has been widely adopted for plant genetic engineering and the study of gene function(Krenek et al.,2015).This method is prevalent in the genetic transformation of herbaceous plants,with notable applications in species such as Arabidopsis(Yin et al.,2024),soybean(Zhang et al.,2024),rice(Zhang et al.,2020),and Chinese cabbage(Li et al.,2021).However,its application in fruit trees is limited.This is primarily due to their long growth cycles and lack of rapid,efficient,and stable transgenic systems,which severely hinders foundational research involving plant genetic transformation(Mei et al.,2024).Furthermore,for subtropical fruit trees,the presence of recalcitrant seeds adds an extra layer of difficulty to genetic transformation(Umarani et al.,2015),as most methods rely on seed germination as a basis for transformation.展开更多
Proteus mirabilis is abundant in soil and water,and although it is part of the normal human intestinal flora(along with Klebsiella species and Escherichia coli),it is known to cause serious infections in humans,with a...Proteus mirabilis is abundant in soil and water,and although it is part of the normal human intestinal flora(along with Klebsiella species and Escherichia coli),it is known to cause serious infections in humans,with a fatality rate of 20%-50%[1].Proteus mirabilis is intrinsically resistant to tetracycline,tigecycline,and colistin.The widespread and irregular use of antimicrobial agents,P.mirabilis antimicrobial resistance has mirabilis.They acquire antimicrobial resistance(AMR)by capturing plasmids,transposons,or other mobile elements harboring antimicrobial resistance genes.This mechanism allows the rapid selection and transmission of numerous AMR genes in clinical,veterinary,food production,transportation,and environmental settings.展开更多
当前，现代检验医学已步入了一个以自动化、信息化、网络化为主要特征的新时期。检验水平和检测手段日新月异，临床检验工作日趋自动化、规范化、信息化，因而建立一套功能强大的实验室信息系统（laboratory information system，LIS）...当前，现代检验医学已步入了一个以自动化、信息化、网络化为主要特征的新时期。检验水平和检测手段日新月异，临床检验工作日趋自动化、规范化、信息化，因而建立一套功能强大的实验室信息系统（laboratory information system，LIS）是现代临床实验室必不可少的。如今的LIS已不仅仅局限于标本管理、数据采集、报告打印等简单的功能，信息技术的发展、医疗体制改革的要求以及各种严格的行业规范，将LIS推向了前所未有的高度。展开更多
基金funded by the Key-Area Research and Development Program of Guangdong Province(Grant No.2022B0202070002)the Guangxi Science and Technology Major Program(Grant No.GuikeAA23023007-2)+1 种基金the Guangdong Province Modern Agricultural Industry Technology System Innovation Team Construction Project(2024CXTD19)Guangdong Basic and Applied Basic Research Foundation(Grant No.2023A1515010303)。
文摘Agrobacterium tumefaciens-mediated transformation has been widely adopted for plant genetic engineering and the study of gene function(Krenek et al.,2015).This method is prevalent in the genetic transformation of herbaceous plants,with notable applications in species such as Arabidopsis(Yin et al.,2024),soybean(Zhang et al.,2024),rice(Zhang et al.,2020),and Chinese cabbage(Li et al.,2021).However,its application in fruit trees is limited.This is primarily due to their long growth cycles and lack of rapid,efficient,and stable transgenic systems,which severely hinders foundational research involving plant genetic transformation(Mei et al.,2024).Furthermore,for subtropical fruit trees,the presence of recalcitrant seeds adds an extra layer of difficulty to genetic transformation(Umarani et al.,2015),as most methods rely on seed germination as a basis for transformation.
基金the National Key Research and Development Program of China[grant number 2021YFC2302002]for financial support.
文摘Proteus mirabilis is abundant in soil and water,and although it is part of the normal human intestinal flora(along with Klebsiella species and Escherichia coli),it is known to cause serious infections in humans,with a fatality rate of 20%-50%[1].Proteus mirabilis is intrinsically resistant to tetracycline,tigecycline,and colistin.The widespread and irregular use of antimicrobial agents,P.mirabilis antimicrobial resistance has mirabilis.They acquire antimicrobial resistance(AMR)by capturing plasmids,transposons,or other mobile elements harboring antimicrobial resistance genes.This mechanism allows the rapid selection and transmission of numerous AMR genes in clinical,veterinary,food production,transportation,and environmental settings.
文摘当前，现代检验医学已步入了一个以自动化、信息化、网络化为主要特征的新时期。检验水平和检测手段日新月异，临床检验工作日趋自动化、规范化、信息化，因而建立一套功能强大的实验室信息系统（laboratory information system，LIS）是现代临床实验室必不可少的。如今的LIS已不仅仅局限于标本管理、数据采集、报告打印等简单的功能，信息技术的发展、医疗体制改革的要求以及各种严格的行业规范，将LIS推向了前所未有的高度。
