Magnesium(Mg)alloys have excellent biocompatibility and biodegradability,making them promising for clinical applications.However,their rapid degradation compared to bone healing limits their effectiveness.In this stud...Magnesium(Mg)alloys have excellent biocompatibility and biodegradability,making them promising for clinical applications.However,their rapid degradation compared to bone healing limits their effectiveness.In this study,low-intensity pulsed ultrasound(LIPUS),widely used clinically to promote bone healing,was combined with Mg alloy scaffolds to evaluate scaffold degradation under dynamic conditions,in vitro using Hanks’balanced salt solution+BSA solution and in vivo in the femoral condyles of male SD rats.Results showed that LIPUS accelerated the initial degradation of the scaffold in both in vivo and in vitro experiments.In vitro,LIPUS increased BSA adsorption on scaffold surfaces,with adsorption increasing alongside LIPUS intensity.Limited BSA replenishment led to a thin organic-inorganic film that provided weak resistance to corrosive ions,accelerating degradation.Cavitation induced by LIPUS caused microbubble collapse,detaching Ca-P salts from scaffold surfaces.In vivo,LIPUS enhanced cell membrane permeability and activity,promoting the secretion of substances that formed a thicker organic-inorganic composite layer.Continuous material replenishment in the in vivo environment ensured the protective effect of this layer against corrosive ions,while embedded Ca-P salts were less likely to detach.In addition,LIPUS promotes bone modification.These findings highlight the potential of combining LIPUS with Mg alloys to regulate scaffold degradation,offering innovative strategies for clinical bone repair.展开更多
Objective Low-intensity pulsed ultrasound （LIPUS） has been reported to enhance proliferation and to alter protein production in various kinds of cells. In the present study, we measured the neurites length after LIP...Objective Low-intensity pulsed ultrasound （LIPUS） has been reported to enhance proliferation and to alter protein production in various kinds of cells. In the present study, we measured the neurites length after LIPUS treatment to define the effectiveness of LIPUS stimulation on neurons, and then we examined the acticity of GSK-3β to study the intracellular mechanism of neurite＇s outgrowth. Methods LIPUS was applied to cultured primary rat cortical neurons for 5 minutes every day with spatial- and temporal average intensities （SATA） of 10 mW/cm^2, a pulse width of 200 microseconds, a repetition rate of 1.5 KHz, and an operation frequency of 1 MHz. Neurons were photographed on the third day after LIPUS treatment and harvested at third, seventh, and tenth days for immnoblot and semi-quantitative RT-PCR analysis. Results Morphology change showed that neurite extension was enhanced by LIPUS. There was also a remarkable decrease of proteins, including p-Akt, p-GSK-3β, and p-CRMP-2, observed on the seventh and tenth days, and of GSK-3β mRNA expression, observed on the seventh day, in neurons treated with LIPUS. Conclusion LIPUS can enhance elongation of neurites and it is possible through the decreased expression of GSK-3β.展开更多
Excellent firm bonding between the biomaterials and bone tissue (osseointegration and osteo-conductivity) has been desired for the stability in vivo of dental implants and artificial joints. Much has been learned abou...Excellent firm bonding between the biomaterials and bone tissue (osseointegration and osteo-conductivity) has been desired for the stability in vivo of dental implants and artificial joints. Much has been learned about this concept, which has led to significant improvements in the design and surface modification of implants in the field of implant dentistry, orthopedic surgery. We have already reported that low-intensity pulsed ultrasound (LIPUS) irradiation can accelerate the bone bonding ability of the bio-conductive materials such as bioactive titanium and hydroxyapatite implant. However, it is still unclear whether the LIPUS could have same effect to different types of the bioactive-materials. Therefore, in this study, the differences of bone-like hydroxyapatite formation on some kind of hydroxyapatite surface in simulated body fluid (SBF) under the LIPUS irradiation were investigated. Two kinds of hydroxyapatite samples immersed in SBF was exposed to ultrasound waves, the bone-like apatite on the surface was analyzed by Scanning electron microscopy and X-ray diffraction. As a result, the enhancement of hydroxyapatite formation on the surface by LIPUS was confirmed, the initial epitaxial nucleation and crystal growth of apatite depended on crystal structure of the surface of matrix materials.展开更多
As a biopic focusing on the people’s writer Liu Qing,the movie Liu Qing shows the creative process of Liu Qing’s rooting in the countryside and sharing the fate of peasants through the fusion of realist aesthetics a...As a biopic focusing on the people’s writer Liu Qing,the movie Liu Qing shows the creative process of Liu Qing’s rooting in the countryside and sharing the fate of peasants through the fusion of realist aesthetics and poetic expression.Contemporary writer’s biography movie is almost blank in the domestic movie history due to its special subject matter.The creation of Liu Qing required balancing the tension between historical truth,artistic aesthetics,and policy scrutiny.By deconstructing the multiple identities of the writer,reconstructing the logic of vernacular narrative,and exploring the innovation of melodramatic genre,the director has created the image of a people’s writer rooted in the earth.Combining the perspective of director Tian Bo,this article discusses how to construct a writer’s image on the screen with both depth of thought and humanistic temperature,and reveals the revelation of Liu Qing’s spirit to contemporary literary creation.展开更多
As a doctor,being away from patients is like being a fish out of water.As long as I have breath,I will do something for my patients.-Life motto of Dr.Zijiang Liu As a pioneer and founder of interventional radiology in...As a doctor,being away from patients is like being a fish out of water.As long as I have breath,I will do something for my patients.-Life motto of Dr.Zijiang Liu As a pioneer and founder of interventional radiology in China,Professor Zijiang Liu(1925-2003)paved new pathways for disciplinary construction and clinical practice in this field.Professor Liu brought hope to countless patients with his exceptional wisdom and outstanding dedication.Born in Shaoxing,Zhejiang Province,on 4 October 1925,Professor Liu was inspired to pursue a career in medicine after suffering from tuberculosis in his youth.展开更多
On 18 December 2024,Liu Hongcai,Vice-President of CAFIU met with Seiji Yamamoto,Director General of the Broad National Alliance for Independence,Peace and Democracy of Japan.The two sides exchanged views on China-Japa...On 18 December 2024,Liu Hongcai,Vice-President of CAFIU met with Seiji Yamamoto,Director General of the Broad National Alliance for Independence,Peace and Democracy of Japan.The two sides exchanged views on China-Japan relations,people-to-people exchanges and cooperation between the two organisations.展开更多
On 11th August,Masaharu Yamamoto,Director of the Affairs Bureau of the National Federation of Independent Peace and Democracy of Japan(National Federation),paid a courtesy call to CAFIU Vice-President Liu Hongcai,exch...On 11th August,Masaharu Yamamoto,Director of the Affairs Bureau of the National Federation of Independent Peace and Democracy of Japan(National Federation),paid a courtesy call to CAFIU Vice-President Liu Hongcai,exchanging views on people-to-people contacts between China and Japan and future cooperation between the two sides.展开更多
On 3Oth July,Yukihisa Fujita,Chairman of Moral Re-Armament Japan(MRA Japan),paid a courtesy call to Liu Hongcai,Vice-President of the Chinese Association for International Understanding(CAFIU).Both sides exchanged vie...On 3Oth July,Yukihisa Fujita,Chairman of Moral Re-Armament Japan(MRA Japan),paid a courtesy call to Liu Hongcai,Vice-President of the Chinese Association for International Understanding(CAFIU).Both sides exchanged views on peopleto-people exchanges between China and Japan and relations between the two organisations.展开更多
利用三阶混沌系统构造了一种新的微弱信号检测系统——类Liu系统,对类Liu系统进行了深度的理论分析.类Liu系统中,当输入待测信号幅值大于某临界值时,系统可达到平衡点S0,S0中系统变量x平衡于摄动力信号,系统变量y,z收敛于零态,且S0对应...利用三阶混沌系统构造了一种新的微弱信号检测系统——类Liu系统,对类Liu系统进行了深度的理论分析.类Liu系统中,当输入待测信号幅值大于某临界值时,系统可达到平衡点S0,S0中系统变量x平衡于摄动力信号,系统变量y,z收敛于零态,且S0对应的Lyapunov指数小于零.通过Matlab仿真、Multisim电路仿真以及实际电路证明了类Liu系统的周期态收敛性及广域检测性,解决了传统Duffing系统进行微弱信号检测时周期态不收敛、只能进行窄域检测等问题,同时谱级信噪比范围仍可达-46.57 d B.类Liu系统采用了全新的设计理念,具有较高的实用价值,对未来海洋物联网中的水声通信有一定参考价值.展开更多
基金supported by Project of Zhongyuan Critical Metals Laboratory(GJJSGFYQ202406)National Natural Science Foundation of China(51701184,51671175)Young Backbone Teachers Foundation of Zhengzhou University.
文摘Magnesium(Mg)alloys have excellent biocompatibility and biodegradability,making them promising for clinical applications.However,their rapid degradation compared to bone healing limits their effectiveness.In this study,low-intensity pulsed ultrasound(LIPUS),widely used clinically to promote bone healing,was combined with Mg alloy scaffolds to evaluate scaffold degradation under dynamic conditions,in vitro using Hanks’balanced salt solution+BSA solution and in vivo in the femoral condyles of male SD rats.Results showed that LIPUS accelerated the initial degradation of the scaffold in both in vivo and in vitro experiments.In vitro,LIPUS increased BSA adsorption on scaffold surfaces,with adsorption increasing alongside LIPUS intensity.Limited BSA replenishment led to a thin organic-inorganic film that provided weak resistance to corrosive ions,accelerating degradation.Cavitation induced by LIPUS caused microbubble collapse,detaching Ca-P salts from scaffold surfaces.In vivo,LIPUS enhanced cell membrane permeability and activity,promoting the secretion of substances that formed a thicker organic-inorganic composite layer.Continuous material replenishment in the in vivo environment ensured the protective effect of this layer against corrosive ions,while embedded Ca-P salts were less likely to detach.In addition,LIPUS promotes bone modification.These findings highlight the potential of combining LIPUS with Mg alloys to regulate scaffold degradation,offering innovative strategies for clinical bone repair.
基金supported by Natural Science Foundation of Beijing,ChinaGrant number:5072020.
文摘Objective Low-intensity pulsed ultrasound （LIPUS） has been reported to enhance proliferation and to alter protein production in various kinds of cells. In the present study, we measured the neurites length after LIPUS treatment to define the effectiveness of LIPUS stimulation on neurons, and then we examined the acticity of GSK-3β to study the intracellular mechanism of neurite＇s outgrowth. Methods LIPUS was applied to cultured primary rat cortical neurons for 5 minutes every day with spatial- and temporal average intensities （SATA） of 10 mW/cm^2, a pulse width of 200 microseconds, a repetition rate of 1.5 KHz, and an operation frequency of 1 MHz. Neurons were photographed on the third day after LIPUS treatment and harvested at third, seventh, and tenth days for immnoblot and semi-quantitative RT-PCR analysis. Results Morphology change showed that neurite extension was enhanced by LIPUS. There was also a remarkable decrease of proteins, including p-Akt, p-GSK-3β, and p-CRMP-2, observed on the seventh and tenth days, and of GSK-3β mRNA expression, observed on the seventh day, in neurons treated with LIPUS. Conclusion LIPUS can enhance elongation of neurites and it is possible through the decreased expression of GSK-3β.
文摘Excellent firm bonding between the biomaterials and bone tissue (osseointegration and osteo-conductivity) has been desired for the stability in vivo of dental implants and artificial joints. Much has been learned about this concept, which has led to significant improvements in the design and surface modification of implants in the field of implant dentistry, orthopedic surgery. We have already reported that low-intensity pulsed ultrasound (LIPUS) irradiation can accelerate the bone bonding ability of the bio-conductive materials such as bioactive titanium and hydroxyapatite implant. However, it is still unclear whether the LIPUS could have same effect to different types of the bioactive-materials. Therefore, in this study, the differences of bone-like hydroxyapatite formation on some kind of hydroxyapatite surface in simulated body fluid (SBF) under the LIPUS irradiation were investigated. Two kinds of hydroxyapatite samples immersed in SBF was exposed to ultrasound waves, the bone-like apatite on the surface was analyzed by Scanning electron microscopy and X-ray diffraction. As a result, the enhancement of hydroxyapatite formation on the surface by LIPUS was confirmed, the initial epitaxial nucleation and crystal growth of apatite depended on crystal structure of the surface of matrix materials.
文摘As a biopic focusing on the people’s writer Liu Qing,the movie Liu Qing shows the creative process of Liu Qing’s rooting in the countryside and sharing the fate of peasants through the fusion of realist aesthetics and poetic expression.Contemporary writer’s biography movie is almost blank in the domestic movie history due to its special subject matter.The creation of Liu Qing required balancing the tension between historical truth,artistic aesthetics,and policy scrutiny.By deconstructing the multiple identities of the writer,reconstructing the logic of vernacular narrative,and exploring the innovation of melodramatic genre,the director has created the image of a people’s writer rooted in the earth.Combining the perspective of director Tian Bo,this article discusses how to construct a writer’s image on the screen with both depth of thought and humanistic temperature,and reveals the revelation of Liu Qing’s spirit to contemporary literary creation.
文摘As a doctor,being away from patients is like being a fish out of water.As long as I have breath,I will do something for my patients.-Life motto of Dr.Zijiang Liu As a pioneer and founder of interventional radiology in China,Professor Zijiang Liu(1925-2003)paved new pathways for disciplinary construction and clinical practice in this field.Professor Liu brought hope to countless patients with his exceptional wisdom and outstanding dedication.Born in Shaoxing,Zhejiang Province,on 4 October 1925,Professor Liu was inspired to pursue a career in medicine after suffering from tuberculosis in his youth.
文摘On 18 December 2024,Liu Hongcai,Vice-President of CAFIU met with Seiji Yamamoto,Director General of the Broad National Alliance for Independence,Peace and Democracy of Japan.The two sides exchanged views on China-Japan relations,people-to-people exchanges and cooperation between the two organisations.
文摘On 11th August,Masaharu Yamamoto,Director of the Affairs Bureau of the National Federation of Independent Peace and Democracy of Japan(National Federation),paid a courtesy call to CAFIU Vice-President Liu Hongcai,exchanging views on people-to-people contacts between China and Japan and future cooperation between the two sides.
文摘On 3Oth July,Yukihisa Fujita,Chairman of Moral Re-Armament Japan(MRA Japan),paid a courtesy call to Liu Hongcai,Vice-President of the Chinese Association for International Understanding(CAFIU).Both sides exchanged views on peopleto-people exchanges between China and Japan and relations between the two organisations.
文摘利用三阶混沌系统构造了一种新的微弱信号检测系统——类Liu系统,对类Liu系统进行了深度的理论分析.类Liu系统中,当输入待测信号幅值大于某临界值时,系统可达到平衡点S0,S0中系统变量x平衡于摄动力信号,系统变量y,z收敛于零态,且S0对应的Lyapunov指数小于零.通过Matlab仿真、Multisim电路仿真以及实际电路证明了类Liu系统的周期态收敛性及广域检测性,解决了传统Duffing系统进行微弱信号检测时周期态不收敛、只能进行窄域检测等问题,同时谱级信噪比范围仍可达-46.57 d B.类Liu系统采用了全新的设计理念,具有较高的实用价值,对未来海洋物联网中的水声通信有一定参考价值.
