Ligularia jaluensis is an important medicinal and ornamental plant in China.However,the viruses capable of infecting Ligularia jaluensis remains unknown.Here,we identified a novel carlavirus,tentatively named ligulari...Ligularia jaluensis is an important medicinal and ornamental plant in China.However,the viruses capable of infecting Ligularia jaluensis remains unknown.Here,we identified a novel carlavirus,tentatively named ligularia jaluensis carlavirus(LJCV),as well as a known iris severe mosaic virus(ISMV),in L.jaluensis plants displaying chlorosis and yellow ring spot symptoms,using RNA-seq analysis.The LJCV genome consists of an 8497 nt positive-sense,single-stranded RNA[excluding the poly(A)tail],and contains six open reading frames(ORFs).Phylogenetic analyses based on the fulllength genome and RNA-dependent RNA polymerase(RdRp)amino acid(aa)sequences revealed that LJCV clusters within an evolutionary branch alongside known viruses in the Carlavirus genus.The RdRp protein encoded by ORF1 of LJCV shared 45.38%–67.41%identity with the corresponding proteins of eight closely related carlaviruses.ORFs 2–4 constitute the triple gene block(TGB),with TGBp1 and TGBp3 localized in the endoplasmic reticulum(ER),while TGBp2 is localized at plasmodesmata(PD)and facilitates viral intercellular movement,as demonstrated by its ability to complement the potato virus X with movement-deficient mutant(PVX-Δp25-GFP).Additionally,ORF6 encodes a cysteine-rich protein(CRP)that is localized in the chloroplast and functions as a viral pathogenicity factor,inducing severe viral symptoms in the heterologous PVX expression system.Furthermore,we successfully constructed an infectious cDNA clone of LJCV,and found that it can infect Nicotiana benthamiana plants through mechanical inoculation or agrobacterium-mediated infiltration of the LJCV infectious clone.These findings enhance our understanding of the characteristics and host range of carlaviruses,as well as the viruses capable of infecting L.jaluensis.展开更多
From the alcoholic extract of the whole plant of Ligularia virgaurea spp. oligocephala,three novel eremophilane sesquiterpene lactones, 6α, 10α-dihydroxy-1-oxoeremophila- 7(11),8(9)-dien-8, 12-olide, 6β, 10α-dihyd...From the alcoholic extract of the whole plant of Ligularia virgaurea spp. oligocephala,three novel eremophilane sesquiterpene lactones, 6α, 10α-dihydroxy-1-oxoeremophila- 7(11),8(9)-dien-8, 12-olide, 6β, 10α-dihydroxy-1-oxoeremophila-7 (11), 8 (9)-dien-8, 12-olide and10α-diydroxy-1-oxoeremophila-7(11), 8 (9)-dien-8, 12 olide were isolated. Their structure s wereelucidated by various, spectroscopic methods including intensive 2D NMR techniques (COSY,HMQC, HMBC and ~1H-~1H NOESY) and HR-MS.展开更多
A new furanoeremophilane and a new eudesmane were isolated from the roots of Ligularia veitchiana. Their structures were elucidated as 1b, 10b-epoxy-6b-isobutyryloxy-9-oxo-furanoeremophilane and 8a-hydroxy-4 (15), 11...A new furanoeremophilane and a new eudesmane were isolated from the roots of Ligularia veitchiana. Their structures were elucidated as 1b, 10b-epoxy-6b-isobutyryloxy-9-oxo-furanoeremophilane and 8a-hydroxy-4 (15), 11-eudesmadiene by spectroscopic methods.展开更多
Chemical investigation of L. fischeri afforded two new eremophilenolides. which were identified as 6 beta-methoxy-8 beta-hpdroxy-eremophil-7(11)-en-12,8 alpha-olide 1; 6-oxo-8 beta-hydroxy-eremophil-7(11)-en- 12.8 alp...Chemical investigation of L. fischeri afforded two new eremophilenolides. which were identified as 6 beta-methoxy-8 beta-hpdroxy-eremophil-7(11)-en-12,8 alpha-olide 1; 6-oxo-8 beta-hydroxy-eremophil-7(11)-en- 12.8 alpha-olide 2.展开更多
From Ligularia atroviolacea, four new eremophilendiolides, 81$-hydroxy-eremophil-3,7 （11）-dien-12,8α（14,6α）-diolide （1）, 8β-methoxy-eremophil-3,7（11）-dien-12,8α（14,6α）-diolide （2）, 8α-hydroxy-eremoph...From Ligularia atroviolacea, four new eremophilendiolides, 81$-hydroxy-eremophil-3,7 （11）-dien-12,8α（14,6α）-diolide （1）, 8β-methoxy-eremophil-3,7（11）-dien-12,8α（14,6α）-diolide （2）, 8α-hydroxy-eremophil-3,7（11）-dien-12,8lβ（14,6α）-diolide （3） and eremophil-3,7（11）,8-trien-12,8 （14,6α）-diolide （4）, as well as a known diolide （5） were isolated. Their structures were elucidated on the basis of 1D and 2D NMR as well as ESI-MS spectral data.展开更多
Chemical investigation of L. tongolensis afforded two new eremophilenolides, which were identified as 3β-（2＇-methylbutanoyloxy）-8βH-eremophil-7（11）-en-12, 8α（14, 6α）-diolide （1）and 8βH-eremophil-3, 7（11...Chemical investigation of L. tongolensis afforded two new eremophilenolides, which were identified as 3β-（2＇-methylbutanoyloxy）-8βH-eremophil-7（11）-en-12, 8α（14, 6α）-diolide （1）and 8βH-eremophil-3, 7（11）-dien-12, 8α （14, 6α）-diolide （2）. Their structures were established by spectroscopic methods including 2D NMR experiments.展开更多
From the roots of Ligularia macrophylla, a bisesquiterpene, ligumacrophyllal was isolated and its structure was elucidated on the basis of spectroscopic methods.
A new stigmasterol 3, 7, 22-trihydoxystigmast-5-ene (1) and a new eremophilen- olide 8-methoxy-6-angeloyloxyeremophil-7(11)-en-8, 12-olide-14-oic acid (2) were isolated from Ligularia dolichobotrys Diels. Their struc...A new stigmasterol 3, 7, 22-trihydoxystigmast-5-ene (1) and a new eremophilen- olide 8-methoxy-6-angeloyloxyeremophil-7(11)-en-8, 12-olide-14-oic acid (2) were isolated from Ligularia dolichobotrys Diels. Their structures were deduced on the basis of spectral data.展开更多
Two new pyrrolizidines named lankongensisine A (1), B (2) were isolated from the roots of Ligularia lankongensis collected in Lijiang, Yunnan, and their structures were established by spectroscopic analysis.
A new 8-O-4′-type neolignan named narynenol （1） was isolated from the roots of Ligularia narynensis. Its structure and the relative configurations were confirmed by spectroscopic data interpretation. 2007 Zhong J...A new 8-O-4′-type neolignan named narynenol （1） was isolated from the roots of Ligularia narynensis. Its structure and the relative configurations were confirmed by spectroscopic data interpretation. 2007 Zhong Jian Jia. Published by Elsevier B.V. on behalf of Chinese Chemical Society, All rights reserved.展开更多
A new bisabolane sesquiterpene, 5 beta-acetoxy-4 beta, 8-diangeloyloxy-2 beta,3 beta, :10,11-diepoxy-bisabola-7 (14)-ene, was isolated from the roots of Ligularia songarica and its structure was elucidated on the basi...A new bisabolane sesquiterpene, 5 beta-acetoxy-4 beta, 8-diangeloyloxy-2 beta,3 beta, :10,11-diepoxy-bisabola-7 (14)-ene, was isolated from the roots of Ligularia songarica and its structure was elucidated on the basis of spectroscopic methods, especially 2D-NMR techniques.展开更多
A new isopropenyl benzofuran-type tetramer was isolated from the roots of ligularia stenocephala and its structure was established by spectroscopic methods.
基金financially supported by the Hainan Province Science and Technology Special Fund(No.ZDYF2022XDNY240)the National Key Research and Development Program of China(2022YFD1401200).
文摘Ligularia jaluensis is an important medicinal and ornamental plant in China.However,the viruses capable of infecting Ligularia jaluensis remains unknown.Here,we identified a novel carlavirus,tentatively named ligularia jaluensis carlavirus(LJCV),as well as a known iris severe mosaic virus(ISMV),in L.jaluensis plants displaying chlorosis and yellow ring spot symptoms,using RNA-seq analysis.The LJCV genome consists of an 8497 nt positive-sense,single-stranded RNA[excluding the poly(A)tail],and contains six open reading frames(ORFs).Phylogenetic analyses based on the fulllength genome and RNA-dependent RNA polymerase(RdRp)amino acid(aa)sequences revealed that LJCV clusters within an evolutionary branch alongside known viruses in the Carlavirus genus.The RdRp protein encoded by ORF1 of LJCV shared 45.38%–67.41%identity with the corresponding proteins of eight closely related carlaviruses.ORFs 2–4 constitute the triple gene block(TGB),with TGBp1 and TGBp3 localized in the endoplasmic reticulum(ER),while TGBp2 is localized at plasmodesmata(PD)and facilitates viral intercellular movement,as demonstrated by its ability to complement the potato virus X with movement-deficient mutant(PVX-Δp25-GFP).Additionally,ORF6 encodes a cysteine-rich protein(CRP)that is localized in the chloroplast and functions as a viral pathogenicity factor,inducing severe viral symptoms in the heterologous PVX expression system.Furthermore,we successfully constructed an infectious cDNA clone of LJCV,and found that it can infect Nicotiana benthamiana plants through mechanical inoculation or agrobacterium-mediated infiltration of the LJCV infectious clone.These findings enhance our understanding of the characteristics and host range of carlaviruses,as well as the viruses capable of infecting L.jaluensis.
文摘From the alcoholic extract of the whole plant of Ligularia virgaurea spp. oligocephala,three novel eremophilane sesquiterpene lactones, 6α, 10α-dihydroxy-1-oxoeremophila- 7(11),8(9)-dien-8, 12-olide, 6β, 10α-dihydroxy-1-oxoeremophila-7 (11), 8 (9)-dien-8, 12-olide and10α-diydroxy-1-oxoeremophila-7(11), 8 (9)-dien-8, 12 olide were isolated. Their structure s wereelucidated by various, spectroscopic methods including intensive 2D NMR techniques (COSY,HMQC, HMBC and ~1H-~1H NOESY) and HR-MS.
基金supported by the National Natural Science Foundation of China(No.29972017)
文摘A new furanoeremophilane and a new eudesmane were isolated from the roots of Ligularia veitchiana. Their structures were elucidated as 1b, 10b-epoxy-6b-isobutyryloxy-9-oxo-furanoeremophilane and 8a-hydroxy-4 (15), 11-eudesmadiene by spectroscopic methods.
文摘Chemical investigation of L. fischeri afforded two new eremophilenolides. which were identified as 6 beta-methoxy-8 beta-hpdroxy-eremophil-7(11)-en-12,8 alpha-olide 1; 6-oxo-8 beta-hydroxy-eremophil-7(11)-en- 12.8 alpha-olide 2.
文摘From Ligularia atroviolacea, four new eremophilendiolides, 81$-hydroxy-eremophil-3,7 （11）-dien-12,8α（14,6α）-diolide （1）, 8β-methoxy-eremophil-3,7（11）-dien-12,8α（14,6α）-diolide （2）, 8α-hydroxy-eremophil-3,7（11）-dien-12,8lβ（14,6α）-diolide （3） and eremophil-3,7（11）,8-trien-12,8 （14,6α）-diolide （4）, as well as a known diolide （5） were isolated. Their structures were elucidated on the basis of 1D and 2D NMR as well as ESI-MS spectral data.
基金This work was supported by the NNSFC(No.20372029 and No,20021001)by the Key Project of Chinese Ministry of Education(No.104178).
文摘Chemical investigation of L. tongolensis afforded two new eremophilenolides, which were identified as 3β-（2＇-methylbutanoyloxy）-8βH-eremophil-7（11）-en-12, 8α（14, 6α）-diolide （1）and 8βH-eremophil-3, 7（11）-dien-12, 8α （14, 6α）-diolide （2）. Their structures were established by spectroscopic methods including 2D NMR experiments.
基金This work was supported by the NNSFC (No. 29972017).
文摘From the roots of Ligularia macrophylla, a bisesquiterpene, ligumacrophyllal was isolated and its structure was elucidated on the basis of spectroscopic methods.
文摘A new stigmasterol 3, 7, 22-trihydoxystigmast-5-ene (1) and a new eremophilen- olide 8-methoxy-6-angeloyloxyeremophil-7(11)-en-8, 12-olide-14-oic acid (2) were isolated from Ligularia dolichobotrys Diels. Their structures were deduced on the basis of spectral data.
文摘Two new pyrrolizidines named lankongensisine A (1), B (2) were isolated from the roots of Ligularia lankongensis collected in Lijiang, Yunnan, and their structures were established by spectroscopic analysis.
文摘A new 8-O-4′-type neolignan named narynenol （1） was isolated from the roots of Ligularia narynensis. Its structure and the relative configurations were confirmed by spectroscopic data interpretation. 2007 Zhong Jian Jia. Published by Elsevier B.V. on behalf of Chinese Chemical Society, All rights reserved.
文摘A new bisabolane sesquiterpene, 5 beta-acetoxy-4 beta, 8-diangeloyloxy-2 beta,3 beta, :10,11-diepoxy-bisabola-7 (14)-ene, was isolated from the roots of Ligularia songarica and its structure was elucidated on the basis of spectroscopic methods, especially 2D-NMR techniques.
基金supoaed by the National Natural Science Foundation of China(No.29972017)
文摘A new isopropenyl benzofuran-type tetramer was isolated from the roots of ligularia stenocephala and its structure was established by spectroscopic methods.
