Waterborne pathogens pose a lifelong threat, necessitating advanced disinfection systemswith state-of-the-art materials. Laser-Induced Graphene (LIG), a 3-dimensional form ofgraphene, is a widely known electrode mater...Waterborne pathogens pose a lifelong threat, necessitating advanced disinfection systemswith state-of-the-art materials. Laser-Induced Graphene (LIG), a 3-dimensional form ofgraphene, is a widely known electrode material for its electrically-induced antimicrobialproperties. However, LIG surfaces exhibit antimicrobial properties exclusively in the presenceof electricity. In this work, copper-doped LIG (Cu-LIG) composite electrodes and filterswere developed with enhanced antimicrobial properties in single-step laser scribing. Thework emphasizes the optimization of copper doping with LIG for both electrical and nonelectrical-based disinfection. The copper doping was optimized to a minimal concentration(∼1%) just to enhance the electrochemical properties of LIG. Furthermore, the excess additionof copper was helpful towards non-electricity-based treatment without significantleaching. The prepared surfaces were tested in both electrodes and filter configuration andshowed excellent antibacterial and antiviral activity against mixed bacterial culture and amodel enteric virus, MS2 bacteriophage. On the application of 2.5 V with Cu-LIG electrodes,6-log removal of bacteria and virus was achieved. Furthermore, the membrane-based electroconductivefilters were tested in a flow-through configuration and demonstrated 6-logremoval at 2.5 V with a flux of ∼ 500 (L·m^(2))/h with both bacteria and viruses at minimumenergy expense. Additionally, reactive oxygen species scavenging and hydrogen peroxidegeneration experiments have confirmed the role of electrical effects and indirect oxidationon the inactivation mechanism. The prepared Cu-LIG composite surfaces showed potentialfor environmental remediation applications.展开更多
目的：运用生物信息学相关软件,预测欧洲女贞花粉主要过敏原Lig v 1的理化性质和结构,为Lig v 1重组表达系统的选择和过敏原性改造提供参考。方法：查询文献获得Lig v 1的氨基酸序列,运用生物信息学软件分析其理化性质（Prot Param）、...目的：运用生物信息学相关软件,预测欧洲女贞花粉主要过敏原Lig v 1的理化性质和结构,为Lig v 1重组表达系统的选择和过敏原性改造提供参考。方法：查询文献获得Lig v 1的氨基酸序列,运用生物信息学软件分析其理化性质（Prot Param）、信号肽（Signal P 4.1 Server）、跨膜区（TMHMM Server v.2.0）、二级结构（GOR4）、MHCⅡ类抗原表位（Net MHCⅡ2.2 Server）、B细胞抗原表位（ProteanTM5.01）以及系统发生树（MEGA 6）。结果：Lig v 1在大肠杆菌中稳定性较好,不存在信号肽与跨膜区;二级结构中无规则卷曲占大多数;Lig v 1潜在MHCⅡ类抗原表位为30~44区域;B细胞抗原表位既具有连续的氨基酸序列,也具有不连续的氨基酸序列;Lig v 1与欧洲白蜡以及木犀榄的同源蛋白进化距离最近。结论：大肠杆菌是适合重组Lig v 1的表达系统,Lig v 1抗原表位分析为低过敏原性改造提供参考。展开更多
Objective： To investigate the role of simultaneous blockade of CD40/CD40L and B7/CD28 pathways in the immune tolerance via co-expression of sCD40LIg and CTLA4Ig mediated by replication-defective adenovirus. Methods：...Objective： To investigate the role of simultaneous blockade of CD40/CD40L and B7/CD28 pathways in the immune tolerance via co-expression of sCD40LIg and CTLA4Ig mediated by replication-defective adenovirus. Methods： Ad-sCD40LIg- IRES2-CTLA4Ig, replication-defective adenovirus co-expressing sCD40LIg and CTLA4Ig, was constructed and identified. The co-expression of sCD40LIg and CTLA4Ig was evaluated with confocal laser scanning microscope and Western blotting. Skin transplantations of C57BL/6 to BALB/c mice were performed. PBS, Ad-Shuttle-CMV and Ad-sCD40LIg-IRES2-CTLA4Ig were administered. Skin graft survival was monitored and the mRNA expression of both genes was evaluated in the skin allografts. Results： Ad-sCD40LIg-IRESE-CTLA4Ig was constructed successfully and identified. The co-expression of sCD40LIg and CTLA4Ig was identified with confocal laser scanning microscopy and Western blotting. Compared to the skin graft mean survival time （MST） of non-treated group （（5.75±0.71） d） or Ad-Shuttle-CMV-treated group （（5.50±0.53） d）, the skin graft MST was dramatically prolonged in the Ad-sCD40LIg-IRESE-CTLA4Ig-treated group （（ 16.38± 1.19） d, P〈0.001）. The mRNA expression of both genes was detected. Conclusion： Ad-sCD40LIg-IRES2-CTLA4Ig, a replication-defective adenovirus carrying genes encoding sCD40LIg and CTLA4Ig, was constructed. Simultaneous blockade of CD40/CD40L and B7/CD28 costimulatory pathway mediated by replication-defective adenovirus significantly prolonged skin allografi survival in mice.展开更多
Coordination compounds of (±)-2-amino-3-(4-hydroxyphenyl) propionic acid and their mixed ligand complexes with 1,10-phenantroline were synthesized, characterized using electronic and infrared spectral analyses an...Coordination compounds of (±)-2-amino-3-(4-hydroxyphenyl) propionic acid and their mixed ligand complexes with 1,10-phenantroline were synthesized, characterized using electronic and infrared spectral analyses and magnetic susceptibility. The compounds were evaluated for antimicrobial and antioxidant activities. Four different assays were applied for evaluating antioxidant capacity of the compounds. The results obtained indicated a diametric square planar geometry for both cobalt (±)-2-amino-3-(4-hydroxyphenyl) propionic acid complex and its mixed ligand complex. It was suggested that for the binary cobalt(II) complex, the phenolic substituent coordinated with neighbouring central metal ions. However, for the ternary cobalt(II) complex it was suggested it was deprotonated. Octahedral geometry was proposed for both copper complexes. Square planar geometry was indicated for the nickel?(±)-2-amino-3-(4-hydroxyphenyl) propionic acid complex and a mixture of square planar and octahedral geometry for the nickel mixed ligand complex. The cobalt mixed ligand complex elicited the highest activity for all the antioxidant assays. In most cases the binary complexes exhibited better antimicrobial activities relative to their ternary counterparts.展开更多
基金supported by the DST(DST-WTIDST/TMD/EWO/WTI/DM/2021/283,DST-SYST,and DSTinspire faculty award)and Science and Engineering Research Board(SERB+1 种基金No.CRG/2019/005280)the Indian Institute of Technology Bombay(IITB)for their funding support for this work.ND and AMN acknowledge the Ministry of Education,India,for their doctoral fellowship support.We thank Prof.Suparna Mukherjee at IIT Bombay for kindly providing us with the host E.coli C3000(No.ATCC 15597).
文摘Waterborne pathogens pose a lifelong threat, necessitating advanced disinfection systemswith state-of-the-art materials. Laser-Induced Graphene (LIG), a 3-dimensional form ofgraphene, is a widely known electrode material for its electrically-induced antimicrobialproperties. However, LIG surfaces exhibit antimicrobial properties exclusively in the presenceof electricity. In this work, copper-doped LIG (Cu-LIG) composite electrodes and filterswere developed with enhanced antimicrobial properties in single-step laser scribing. Thework emphasizes the optimization of copper doping with LIG for both electrical and nonelectrical-based disinfection. The copper doping was optimized to a minimal concentration(∼1%) just to enhance the electrochemical properties of LIG. Furthermore, the excess additionof copper was helpful towards non-electricity-based treatment without significantleaching. The prepared surfaces were tested in both electrodes and filter configuration andshowed excellent antibacterial and antiviral activity against mixed bacterial culture and amodel enteric virus, MS2 bacteriophage. On the application of 2.5 V with Cu-LIG electrodes,6-log removal of bacteria and virus was achieved. Furthermore, the membrane-based electroconductivefilters were tested in a flow-through configuration and demonstrated 6-logremoval at 2.5 V with a flux of ∼ 500 (L·m^(2))/h with both bacteria and viruses at minimumenergy expense. Additionally, reactive oxygen species scavenging and hydrogen peroxidegeneration experiments have confirmed the role of electrical effects and indirect oxidationon the inactivation mechanism. The prepared Cu-LIG composite surfaces showed potentialfor environmental remediation applications.
文摘目的：运用生物信息学相关软件,预测欧洲女贞花粉主要过敏原Lig v 1的理化性质和结构,为Lig v 1重组表达系统的选择和过敏原性改造提供参考。方法：查询文献获得Lig v 1的氨基酸序列,运用生物信息学软件分析其理化性质（Prot Param）、信号肽（Signal P 4.1 Server）、跨膜区（TMHMM Server v.2.0）、二级结构（GOR4）、MHCⅡ类抗原表位（Net MHCⅡ2.2 Server）、B细胞抗原表位（ProteanTM5.01）以及系统发生树（MEGA 6）。结果：Lig v 1在大肠杆菌中稳定性较好,不存在信号肽与跨膜区;二级结构中无规则卷曲占大多数;Lig v 1潜在MHCⅡ类抗原表位为30~44区域;B细胞抗原表位既具有连续的氨基酸序列,也具有不连续的氨基酸序列;Lig v 1与欧洲白蜡以及木犀榄的同源蛋白进化距离最近。结论：大肠杆菌是适合重组Lig v 1的表达系统,Lig v 1抗原表位分析为低过敏原性改造提供参考。
基金Project (No. 30371416) supported by the National Natural ScienceFoundation of China
文摘Objective： To investigate the role of simultaneous blockade of CD40/CD40L and B7/CD28 pathways in the immune tolerance via co-expression of sCD40LIg and CTLA4Ig mediated by replication-defective adenovirus. Methods： Ad-sCD40LIg- IRES2-CTLA4Ig, replication-defective adenovirus co-expressing sCD40LIg and CTLA4Ig, was constructed and identified. The co-expression of sCD40LIg and CTLA4Ig was evaluated with confocal laser scanning microscope and Western blotting. Skin transplantations of C57BL/6 to BALB/c mice were performed. PBS, Ad-Shuttle-CMV and Ad-sCD40LIg-IRES2-CTLA4Ig were administered. Skin graft survival was monitored and the mRNA expression of both genes was evaluated in the skin allografts. Results： Ad-sCD40LIg-IRESE-CTLA4Ig was constructed successfully and identified. The co-expression of sCD40LIg and CTLA4Ig was identified with confocal laser scanning microscopy and Western blotting. Compared to the skin graft mean survival time （MST） of non-treated group （（5.75±0.71） d） or Ad-Shuttle-CMV-treated group （（5.50±0.53） d）, the skin graft MST was dramatically prolonged in the Ad-sCD40LIg-IRESE-CTLA4Ig-treated group （（ 16.38± 1.19） d, P〈0.001）. The mRNA expression of both genes was detected. Conclusion： Ad-sCD40LIg-IRES2-CTLA4Ig, a replication-defective adenovirus carrying genes encoding sCD40LIg and CTLA4Ig, was constructed. Simultaneous blockade of CD40/CD40L and B7/CD28 costimulatory pathway mediated by replication-defective adenovirus significantly prolonged skin allografi survival in mice.
文摘Coordination compounds of (±)-2-amino-3-(4-hydroxyphenyl) propionic acid and their mixed ligand complexes with 1,10-phenantroline were synthesized, characterized using electronic and infrared spectral analyses and magnetic susceptibility. The compounds were evaluated for antimicrobial and antioxidant activities. Four different assays were applied for evaluating antioxidant capacity of the compounds. The results obtained indicated a diametric square planar geometry for both cobalt (±)-2-amino-3-(4-hydroxyphenyl) propionic acid complex and its mixed ligand complex. It was suggested that for the binary cobalt(II) complex, the phenolic substituent coordinated with neighbouring central metal ions. However, for the ternary cobalt(II) complex it was suggested it was deprotonated. Octahedral geometry was proposed for both copper complexes. Square planar geometry was indicated for the nickel?(±)-2-amino-3-(4-hydroxyphenyl) propionic acid complex and a mixture of square planar and octahedral geometry for the nickel mixed ligand complex. The cobalt mixed ligand complex elicited the highest activity for all the antioxidant assays. In most cases the binary complexes exhibited better antimicrobial activities relative to their ternary counterparts.
